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The map label for this gene is glpF [H]

Identifier: 15898414

GI number: 15898414

Start: 1448024

End: 1448893

Strand: Direct

Name: glpF [H]

Synonym: SSO1601

Alternate gene names: 15898414

Gene position: 1448024-1448893 (Clockwise)

Preceding gene: 15898413

Following gene: 15898418

Centisome position: 48.39

GC content: 40.23

Gene sequence:

>870_bases
ATGTCTGTGGTTTTGAAGGACTCTTTATGGAGATATTTCGCTGAATTTGTAGGAACTTTCATACTAATACTATTCGGAGA
TGGTGCTATAGTAGCCTCAACACTAAGTAGCCAACCCAGTTTCGGATTTATTATGGTAAGCTGGGGATTTGGAATAGTGT
TAGCGATTTATGCTGTTGGTCCAATTAGCGGAGCTCATATAAACCCTAATGTAACGTTAGCGTTTGCTGTAACTAAGCGT
ATTAAGTGGGTTGATGTGATTCCTTACATCGTTTTCCAGATTTTAGGGGCTGCTGCAGCTACAAGCGTATTATTGGTATG
GTGGGGAGATGTAATAACCAGAATAGATCCCCCACCTTTTCTATACGCTGATATAGGAGCGGCTTTCTTTACTCAATATC
CTGAGCCCGGTTTTTGGCCTCAATACTGGCCTAAAAGCTATTTACCTAACGTTAGTAGCGCAGGTATTTTATACTCTCAA
GTAAATCAAATATTTCCCTTATGGAAGGGAGCCTTTGCTGAGGCATTAATGACCTTTTTGTTACTGTTGATAGTAGTAGC
AGTTACGGATCCAGATTCACCCTTCTATAGCCAGTCATTAGCTCCGTGGGCAATAGGTATTGGATATGTAATGCCCTTCT
TACTTTTCGAAGCTCAGTTAACGGGAGGGATGATAAATGAGGCTAGAAGTTGGGGACCTATGCTTGCTTTATATCTATTC
GGTTATAGAACTGGTGCATTTAACTTTAGGGGAGAGTACTTCTATGTTTATGGTATTCCAGATTTTATAGGGGGCATACT
TGGAGCTCTGTTCTGGGACCATGTATTAAAGCCTTATCTTAGGTTCGTAAAAAACATTAACAAAAAGTAG

Upstream 100 bases:

>100_bases
AAGTATGAGTGAGGAGAAAAGGAGGGCATTGTACTCTGGGTGGAAGGAGGCTGTAAAGAGAGCTATGGGATGGGCTAAAG
TTGTAGGAGGTCAAGTTTAA

Downstream 100 bases:

>100_bases
TGATTTTTATCAGTATAATAATAAGACTTTTTTTAACTCAGTTCTATGCTTAGCAGTTTATGTTTTTCTCTTACGTTTAA
ACAATTTCTTTATCTCTCCA

Product: transposon ISC1229 Orf1

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSVVLKDSLWRYFAEFVGTFILILFGDGAIVASTLSSQPSFGFIMVSWGFGIVLAIYAVGPISGAHINPNVTLAFAVTKR
IKWVDVIPYIVFQILGAAAATSVLLVWWGDVITRIDPPPFLYADIGAAFFTQYPEPGFWPQYWPKSYLPNVSSAGILYSQ
VNQIFPLWKGAFAEALMTFLLLLIVVAVTDPDSPFYSQSLAPWAIGIGYVMPFLLFEAQLTGGMINEARSWGPMLALYLF
GYRTGAFNFRGEYFYVYGIPDFIGGILGALFWDHVLKPYLRFVKNINKK

Sequences:

>Translated_289_residues
MSVVLKDSLWRYFAEFVGTFILILFGDGAIVASTLSSQPSFGFIMVSWGFGIVLAIYAVGPISGAHINPNVTLAFAVTKR
IKWVDVIPYIVFQILGAAAATSVLLVWWGDVITRIDPPPFLYADIGAAFFTQYPEPGFWPQYWPKSYLPNVSSAGILYSQ
VNQIFPLWKGAFAEALMTFLLLLIVVAVTDPDSPFYSQSLAPWAIGIGYVMPFLLFEAQLTGGMINEARSWGPMLALYLF
GYRTGAFNFRGEYFYVYGIPDFIGGILGALFWDHVLKPYLRFVKNINKK
>Mature_288_residues
SVVLKDSLWRYFAEFVGTFILILFGDGAIVASTLSSQPSFGFIMVSWGFGIVLAIYAVGPISGAHINPNVTLAFAVTKRI
KWVDVIPYIVFQILGAAAATSVLLVWWGDVITRIDPPPFLYADIGAAFFTQYPEPGFWPQYWPKSYLPNVSSAGILYSQV
NQIFPLWKGAFAEALMTFLLLLIVVAVTDPDSPFYSQSLAPWAIGIGYVMPFLLFEAQLTGGMINEARSWGPMLALYLFG
YRTGAFNFRGEYFYVYGIPDFIGGILGALFWDHVLKPYLRFVKNINKK

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=286, Percent_Identity=28.6713286713287, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI4826645, Length=280, Percent_Identity=26.0714285714286, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI22538420, Length=262, Percent_Identity=24.4274809160305, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1790362, Length=279, Percent_Identity=29.3906810035842, Blast_Score=78, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71992966, Length=262, Percent_Identity=23.6641221374046, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=243, Percent_Identity=29.2181069958848, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 32216; Mature: 32085

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVLKDSLWRYFAEFVGTFILILFGDGAIVASTLSSQPSFGFIMVSWGFGIVLAIYAVG
CCEEEHHHHHHHHHHHHHHHEEHHCCCCHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHC
PISGAHINPNVTLAFAVTKRIKWVDVIPYIVFQILGAAAATSVLLVWWGDVITRIDPPPF
CCCCCEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LYADIGAAFFTQYPEPGFWPQYWPKSYLPNVSSAGILYSQVNQIFPLWKGAFAEALMTFL
EHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLIVVAVTDPDSPFYSQSLAPWAIGIGYVMPFLLFEAQLTGGMINEARSWGPMLALYLF
HHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
GYRTGAFNFRGEYFYVYGIPDFIGGILGALFWDHVLKPYLRFVKNINKK
HHHCCCEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SVVLKDSLWRYFAEFVGTFILILFGDGAIVASTLSSQPSFGFIMVSWGFGIVLAIYAVG
CEEEHHHHHHHHHHHHHHHEEHHCCCCHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHC
PISGAHINPNVTLAFAVTKRIKWVDVIPYIVFQILGAAAATSVLLVWWGDVITRIDPPPF
CCCCCEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LYADIGAAFFTQYPEPGFWPQYWPKSYLPNVSSAGILYSQVNQIFPLWKGAFAEALMTFL
EHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLIVVAVTDPDSPFYSQSLAPWAIGIGYVMPFLLFEAQLTGGMINEARSWGPMLALYLF
HHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
GYRTGAFNFRGEYFYVYGIPDFIGGILGALFWDHVLKPYLRFVKNINKK
HHHCCCEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]