Click here to switch to the map view.

The map label for this gene is glpK1

Identifier: 15898413

GI number: 15898413

Start: 1446518

End: 1448023

Strand: Direct

Name: glpK1

Synonym: SSO1600

Alternate gene names: 15898413

Gene position: 1446518-1448023 (Clockwise)

Preceding gene: 15898412

Following gene: 15898414

Centisome position: 48.34

GC content: 39.04

Gene sequence:

>1506_bases
ATGAATACTATGTCACATAAGTTTGTTCTAGCCCTAGATGAGGGTACTACAAGTGCTAGGGCTATACTCTTCGATAGTGA
CTTAAACATAGTAAACATAGGGCAATATGAATTCCCGCAGTACTATCCTCAACCTGGCTATGTGGAACATGACCCTGAGG
AAATATGGGAAGCTCAAATGTTAGCCGTAAAAAAGGCTATAAGTAAAATAGATGCTAAACAAATAGTAGCAATAGGGATA
ACAAACCAAAGGGAGACAACGGTATTATGGGATGCTAAAAGCGGTAAACCGGTTTACAATGCAATTGTATGGCAGGATAG
AAGAACTTCTCCAATAACGGACTGGCTAAAAGCAAATTACTTTAAAATGATAAAGGACAAAACTGGGTTAGTTCCAGACC
CTTACTTTAGTGCATCTAAGATAAAGTGGATACTTGATAATGTGCCCAATGTTAGGGAGAAAGCAGAAAGAGGAGAAATT
AAGTTCGGGACCCTAGATACTTATTTAATCTGGAGGCTTACTAACGGAAAAGCTCATGTAACTGATTACTCTAACGCTTC
TAGAACAATGCTTTTTAATATAAATAAATTAGAATGTGATAGAGAAATCTTAGAACTTCTAAAAATACCCGAATCTATAT
TGCCAGAGGTTAAACCATCAAGTGAAATTTACGGTTACAGTGAGGCGTTAGGAAACTTAATACCTATATCTGGGGACGCA
GGAGACCAACAAGCAGCTTTATTCGGTCAAGTAGCGTTTAACGTAGGTGAGATAAAAGCTACTTATGGTACCGGTAGTTT
CATTTTAATGAATATAGGCAATAACCCAATACGATCAGAAAACCTCTTAACTACAATAGCTTGGGGTCTTGAGAAGAATA
AGGCAACGTATGCATTAGAGGGGAGTATATTCATAACTGGTGCAGCTGTACAATGGTTTAGGGACGGCTTACGGGCAATA
GACGTTTCAGATGAGATTGAACCTTTAGCCTCAAATGTGGAAGATAATGGCGGTGTCTATTTCGTTCCAGCTTTTGTTGG
ATTGGGAGCTCCTTACTGGGATCCATATGCTAGAGGTCTCATAATAGGCATAACTAGAGGAACTACTAAAGCTCATATAG
CTAGGGCTATTTTAGAATCAATGGCCTATCAAACTAGAGATGTAATTGAGGTAATGCAAAAGGAATCCGGCATCAGTATA
AATTCGCTTAAGGTTGACGGAGGTGCAGCTAAGGATAACCTTTTAATGCAATTCCAAGCTGATATATTAGGTATTAAGGT
AATCAGACCTAAAGTTATGGAAACTACTTCTATGGGAGTTGCAATGCTAGCAGGTTTAGGTGTAGGCTTATGGAACTCTT
TAGAGGAATTAAGGAGCATATGGAAGGTTGATAAGGAATTCATTCCAAGTATGAGTGAGGAGAAAAGGAGGGCATTGTAC
TCTGGGTGGAAGGAGGCTGTAAAGAGAGCTATGGGATGGGCTAAAGTTGTAGGAGGTCAAGTTTAA

Upstream 100 bases:

>100_bases
CGTTATCCTAAATTTTATTTATGGAGAATGCATTGTATCATCTTTATAAACTTAACTTCACAATTAAATATTAAAATTTA
AATATATGTATACATAAATA

Downstream 100 bases:

>100_bases
ATGTCTGTGGTTTTGAAGGACTCTTTATGGAGATATTTCGCTGAATTTGTAGGAACTTTCATACTAATACTATTCGGAGA
TGGTGCTATAGTAGCCTCAA

Product: transporter/facilitator

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase 1; Glycerokinase 1; GK 1

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MNTMSHKFVLALDEGTTSARAILFDSDLNIVNIGQYEFPQYYPQPGYVEHDPEEIWEAQMLAVKKAISKIDAKQIVAIGI
TNQRETTVLWDAKSGKPVYNAIVWQDRRTSPITDWLKANYFKMIKDKTGLVPDPYFSASKIKWILDNVPNVREKAERGEI
KFGTLDTYLIWRLTNGKAHVTDYSNASRTMLFNINKLECDREILELLKIPESILPEVKPSSEIYGYSEALGNLIPISGDA
GDQQAALFGQVAFNVGEIKATYGTGSFILMNIGNNPIRSENLLTTIAWGLEKNKATYALEGSIFITGAAVQWFRDGLRAI
DVSDEIEPLASNVEDNGGVYFVPAFVGLGAPYWDPYARGLIIGITRGTTKAHIARAILESMAYQTRDVIEVMQKESGISI
NSLKVDGGAAKDNLLMQFQADILGIKVIRPKVMETTSMGVAMLAGLGVGLWNSLEELRSIWKVDKEFIPSMSEEKRRALY
SGWKEAVKRAMGWAKVVGGQV

Sequences:

>Translated_501_residues
MNTMSHKFVLALDEGTTSARAILFDSDLNIVNIGQYEFPQYYPQPGYVEHDPEEIWEAQMLAVKKAISKIDAKQIVAIGI
TNQRETTVLWDAKSGKPVYNAIVWQDRRTSPITDWLKANYFKMIKDKTGLVPDPYFSASKIKWILDNVPNVREKAERGEI
KFGTLDTYLIWRLTNGKAHVTDYSNASRTMLFNINKLECDREILELLKIPESILPEVKPSSEIYGYSEALGNLIPISGDA
GDQQAALFGQVAFNVGEIKATYGTGSFILMNIGNNPIRSENLLTTIAWGLEKNKATYALEGSIFITGAAVQWFRDGLRAI
DVSDEIEPLASNVEDNGGVYFVPAFVGLGAPYWDPYARGLIIGITRGTTKAHIARAILESMAYQTRDVIEVMQKESGISI
NSLKVDGGAAKDNLLMQFQADILGIKVIRPKVMETTSMGVAMLAGLGVGLWNSLEELRSIWKVDKEFIPSMSEEKRRALY
SGWKEAVKRAMGWAKVVGGQV
>Mature_501_residues
MNTMSHKFVLALDEGTTSARAILFDSDLNIVNIGQYEFPQYYPQPGYVEHDPEEIWEAQMLAVKKAISKIDAKQIVAIGI
TNQRETTVLWDAKSGKPVYNAIVWQDRRTSPITDWLKANYFKMIKDKTGLVPDPYFSASKIKWILDNVPNVREKAERGEI
KFGTLDTYLIWRLTNGKAHVTDYSNASRTMLFNINKLECDREILELLKIPESILPEVKPSSEIYGYSEALGNLIPISGDA
GDQQAALFGQVAFNVGEIKATYGTGSFILMNIGNNPIRSENLLTTIAWGLEKNKATYALEGSIFITGAAVQWFRDGLRAI
DVSDEIEPLASNVEDNGGVYFVPAFVGLGAPYWDPYARGLIIGITRGTTKAHIARAILESMAYQTRDVIEVMQKESGISI
NSLKVDGGAAKDNLLMQFQADILGIKVIRPKVMETTSMGVAMLAGLGVGLWNSLEELRSIWKVDKEFIPSMSEEKRRALY
SGWKEAVKRAMGWAKVVGGQV

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=506, Percent_Identity=46.6403162055336, Blast_Score=424, Evalue=1e-118,
Organism=Homo sapiens, GI4504007, Length=506, Percent_Identity=46.6403162055336, Blast_Score=424, Evalue=1e-118,
Organism=Homo sapiens, GI42794763, Length=511, Percent_Identity=46.1839530332681, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI41393575, Length=504, Percent_Identity=45.2380952380952, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI88196792, Length=508, Percent_Identity=33.8582677165354, Blast_Score=300, Evalue=2e-81,
Organism=Escherichia coli, GI1790361, Length=494, Percent_Identity=55.8704453441296, Blast_Score=573, Evalue=1e-165,
Organism=Escherichia coli, GI1790005, Length=494, Percent_Identity=25.7085020242915, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1789987, Length=487, Percent_Identity=23.6139630390144, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789138, Length=492, Percent_Identity=24.1869918699187, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787789, Length=491, Percent_Identity=24.847250509165, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17535599, Length=504, Percent_Identity=42.2619047619048, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71986140, Length=522, Percent_Identity=27.7777777777778, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI71986147, Length=273, Percent_Identity=33.6996336996337, Blast_Score=136, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6321755, Length=555, Percent_Identity=34.7747747747748, Blast_Score=314, Evalue=2e-86,
Organism=Drosophila melanogaster, GI17864214, Length=509, Percent_Identity=43.614931237721, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24655555, Length=507, Percent_Identity=41.2228796844181, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24655560, Length=507, Percent_Identity=41.2228796844181, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24655570, Length=507, Percent_Identity=41.2228796844181, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24655574, Length=507, Percent_Identity=41.2228796844181, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24655565, Length=507, Percent_Identity=41.2228796844181, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24654573, Length=434, Percent_Identity=45.1612903225806, Blast_Score=358, Evalue=3e-99,
Organism=Drosophila melanogaster, GI19922058, Length=525, Percent_Identity=32.9523809523809, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI24652871, Length=492, Percent_Identity=34.1463414634146, Blast_Score=269, Evalue=4e-72,
Organism=Drosophila melanogaster, GI24656367, Length=519, Percent_Identity=28.9017341040462, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI21357867, Length=531, Percent_Identity=27.683615819209, Blast_Score=212, Evalue=6e-55,
Organism=Drosophila melanogaster, GI24656372, Length=364, Percent_Identity=29.6703296703297, Blast_Score=158, Evalue=8e-39,
Organism=Drosophila melanogaster, GI45552909, Length=305, Percent_Identity=28.1967213114754, Blast_Score=115, Evalue=6e-26,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK1_SULSO (Q97XW1)

Other databases:

- EMBL:   AE006641
- PIR:   A99319
- RefSeq:   NP_343018.1
- ProteinModelPortal:   Q97XW1
- SMR:   Q97XW1
- GeneID:   1454587
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO1600
- NMPDR:   fig|273057.1.peg.1441
- HOGENOM:   HBG511469
- OMA:   IQRSADV
- PhylomeDB:   Q97XW1
- ProtClustDB:   CLSK268256
- BioCyc:   SSOL273057:SSO1600-MONOMER
- BRENDA:   2.7.1.30
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55682; Mature: 55682

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 16-16 BINDING 20-20 BINDING 84-84 BINDING 135-135 BINDING 242-242 BINDING 264-264 BINDING 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTMSHKFVLALDEGTTSARAILFDSDLNIVNIGQYEFPQYYPQPGYVEHDPEEIWEAQM
CCCCCCEEEEEECCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
LAVKKAISKIDAKQIVAIGITNQRETTVLWDAKSGKPVYNAIVWQDRRTSPITDWLKANY
HHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHH
FKMIKDKTGLVPDPYFSASKIKWILDNVPNVREKAERGEIKFGTLDTYLIWRLTNGKAHV
HHHHHCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHCCCEEEEEECEEEEEEEECCEEEE
TDYSNASRTMLFNINKLECDREILELLKIPESILPEVKPSSEIYGYSEALGNLIPISGDA
EECCCCCCEEEEECCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHCCEEEECCCC
GDQQAALFGQVAFNVGEIKATYGTGSFILMNIGNNPIRSENLLTTIAWGLEKNKATYALE
CCCHHHHHHHHHEEECEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEEEE
GSIFITGAAVQWFRDGLRAIDVSDEIEPLASNVEDNGGVYFVPAFVGLGAPYWDPYARGL
CCEEEEHHHHHHHHCCCEEECCCHHHHHHHCCCCCCCCEEEEEHHHHCCCCCCCCCCCCE
IIGITRGTTKAHIARAILESMAYQTRDVIEVMQKESGISINSLKVDGGAAKDNLLMQFQA
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEC
DILGIKVIRPKVMETTSMGVAMLAGLGVGLWNSLEELRSIWKVDKEFIPSMSEEKRRALY
CCEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SGWKEAVKRAMGWAKVVGGQV
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNTMSHKFVLALDEGTTSARAILFDSDLNIVNIGQYEFPQYYPQPGYVEHDPEEIWEAQM
CCCCCCEEEEEECCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
LAVKKAISKIDAKQIVAIGITNQRETTVLWDAKSGKPVYNAIVWQDRRTSPITDWLKANY
HHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHH
FKMIKDKTGLVPDPYFSASKIKWILDNVPNVREKAERGEIKFGTLDTYLIWRLTNGKAHV
HHHHHCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHCCCEEEEEECEEEEEEEECCEEEE
TDYSNASRTMLFNINKLECDREILELLKIPESILPEVKPSSEIYGYSEALGNLIPISGDA
EECCCCCCEEEEECCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHCCEEEECCCC
GDQQAALFGQVAFNVGEIKATYGTGSFILMNIGNNPIRSENLLTTIAWGLEKNKATYALE
CCCHHHHHHHHHEEECEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEEEE
GSIFITGAAVQWFRDGLRAIDVSDEIEPLASNVEDNGGVYFVPAFVGLGAPYWDPYARGL
CCEEEEHHHHHHHHCCCEEECCCHHHHHHHCCCCCCCCEEEEEHHHHCCCCCCCCCCCCE
IIGITRGTTKAHIARAILESMAYQTRDVIEVMQKESGISINSLKVDGGAAKDNLLMQFQA
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEC
DILGIKVIRPKVMETTSMGVAMLAGLGVGLWNSLEELRSIWKVDKEFIPSMSEEKRRALY
CCEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SGWKEAVKRAMGWAKVVGGQV
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726