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The map label for this gene is pdhD-2 [H]

Identifier: 15898352

GI number: 15898352

Start: 1375603

End: 1376841

Strand: Direct

Name: pdhD-2 [H]

Synonym: SSO1524

Alternate gene names: 15898352

Gene position: 1375603-1376841 (Clockwise)

Preceding gene: 15898351

Following gene: 15898353

Centisome position: 45.97

GC content: 36.32

Gene sequence:

>1239_bases
TTGAAGAATAATATTATGAAAGTTGTAATAATTGGTGCAGGTCCAGCAGGAGTTTACTCAGCATTAACCTTATCCAAACA
CGCAAAAGTTACACTTATAGAAAGGGAAGAAAAACTAGGGGGTACTTGCGTACTTTACGGATGTATACCCACAAAATCCA
TACTAAGCCAGCTTATCATATCTAGGCAAGCATCCAATATGAGTTTAAATACGCTCAGAGAATACGCATTGACTTCAATA
AATACTATAAGTAAGAGTTTAGAACACTTGCTAAATAGTCATGGGATAGAGGTTATTCATGCTAATGGCTTTCTAAGATC
GTCTATGGTTCACGCTTCAAATACTTCACTTGCGGCTGATAAGGTTTTAGTTTCAACCGGAACCAGAAGAGAAAGATTAG
GAAAGGTAAAATTCACAGAGGATTTAGCCTATACTAATGAGGATTATAATAAGGTGGTTATAGTAGGAGGAGATGCAGGT
GGGATAGAATTAGGTTGGATGATGAAGAAACTGGGTAAGGAGGTACATCTTATAGACAAGAACGATTTACTTTTACAAAA
CATTGATAGGACCCTCTCTGAGATAGTTACAAATTTTCTAAGTCAGATAGGGATTAAATTATACTTAGGTAAAAAGGTCT
CTAAAATTGACGAAACGTCTGTTACTCTTGAAGATAATCAAAAGATCTCTGGCGATGCTGTATTCGTAACCTTCGGTCGT
AAACCAAATATTGAAGGATTCGAGGAAATTCCCCACGAGAAGTACATATACGTTGACGAATATTTGAGAACTCAAATTCC
TAACATCTATGCAGCAGGGGATATAATAGGTACGTTTACTGCACATGAAGCCATATATGCTGGTATTATAGCTGCGAAGA
ACATGTTAGGCGAAAAAAGGGAGTTCATCGTAGAAGGAATACCGAAGGTTATATACATTTACCCACAAATAGCCTATGTA
GGTACTACTAATGGTAATTGTGTTACTTTTAACACGTTAAATTTAACTAGAACAATAGTAGAAAGGGAAAGTGAAGGATT
CTTAAAGATATGCGAGAGAGATAACAGAGTAATTGGGGCGGTAGCGTTCATGCCCTATGCAGAAGACGTAATTTCTCTTA
TTTCGGTATTAATACGATACCAAATAAGTTTGAAGGATACGATAGATCTTGTTATGCCTCATCCTTCTTATCTGGAAGCA
ATTACTGAGGCTTTAAATATGTTACAAAGTAAAGGGTAA

Upstream 100 bases:

>100_bases
TAAAGAAACAGAAGAATGATTCAATCATAATATTGAATATACAAGCGAAAGTAGAGGAAGAAGAGAGCGAAGATATCGTA
TAGCATTTATATTCGAGAAA

Downstream 100 bases:

>100_bases
AGAAATGTTCATTGCTGAGTGGAACTCATAGTTTCATTATTAACCTATTTTTGTATTACGAGTATTAGGACGTAATCAAG
TATTCCATTGCCCTAGTCTT

Product: dihydrolipoamide dehydrogenase (pdhD-2)

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MKNNIMKVVIIGAGPAGVYSALTLSKHAKVTLIEREEKLGGTCVLYGCIPTKSILSQLIISRQASNMSLNTLREYALTSI
NTISKSLEHLLNSHGIEVIHANGFLRSSMVHASNTSLAADKVLVSTGTRRERLGKVKFTEDLAYTNEDYNKVVIVGGDAG
GIELGWMMKKLGKEVHLIDKNDLLLQNIDRTLSEIVTNFLSQIGIKLYLGKKVSKIDETSVTLEDNQKISGDAVFVTFGR
KPNIEGFEEIPHEKYIYVDEYLRTQIPNIYAAGDIIGTFTAHEAIYAGIIAAKNMLGEKREFIVEGIPKVIYIYPQIAYV
GTTNGNCVTFNTLNLTRTIVERESEGFLKICERDNRVIGAVAFMPYAEDVISLISVLIRYQISLKDTIDLVMPHPSYLEA
ITEALNMLQSKG

Sequences:

>Translated_412_residues
MKNNIMKVVIIGAGPAGVYSALTLSKHAKVTLIEREEKLGGTCVLYGCIPTKSILSQLIISRQASNMSLNTLREYALTSI
NTISKSLEHLLNSHGIEVIHANGFLRSSMVHASNTSLAADKVLVSTGTRRERLGKVKFTEDLAYTNEDYNKVVIVGGDAG
GIELGWMMKKLGKEVHLIDKNDLLLQNIDRTLSEIVTNFLSQIGIKLYLGKKVSKIDETSVTLEDNQKISGDAVFVTFGR
KPNIEGFEEIPHEKYIYVDEYLRTQIPNIYAAGDIIGTFTAHEAIYAGIIAAKNMLGEKREFIVEGIPKVIYIYPQIAYV
GTTNGNCVTFNTLNLTRTIVERESEGFLKICERDNRVIGAVAFMPYAEDVISLISVLIRYQISLKDTIDLVMPHPSYLEA
ITEALNMLQSKG
>Mature_412_residues
MKNNIMKVVIIGAGPAGVYSALTLSKHAKVTLIEREEKLGGTCVLYGCIPTKSILSQLIISRQASNMSLNTLREYALTSI
NTISKSLEHLLNSHGIEVIHANGFLRSSMVHASNTSLAADKVLVSTGTRRERLGKVKFTEDLAYTNEDYNKVVIVGGDAG
GIELGWMMKKLGKEVHLIDKNDLLLQNIDRTLSEIVTNFLSQIGIKLYLGKKVSKIDETSVTLEDNQKISGDAVFVTFGR
KPNIEGFEEIPHEKYIYVDEYLRTQIPNIYAAGDIIGTFTAHEAIYAGIIAAKNMLGEKREFIVEGIPKVIYIYPQIAYV
GTTNGNCVTFNTLNLTRTIVERESEGFLKICERDNRVIGAVAFMPYAEDVISLISVLIRYQISLKDTIDLVMPHPSYLEA
ITEALNMLQSKG

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=464, Percent_Identity=25.8620689655172, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI50301238, Length=435, Percent_Identity=22.5287356321839, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1786307, Length=455, Percent_Identity=24.1758241758242, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI87082354, Length=354, Percent_Identity=25.7062146892655, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI87081717, Length=454, Percent_Identity=23.3480176211454, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789915, Length=334, Percent_Identity=29.0419161676647, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI32565766, Length=453, Percent_Identity=23.841059602649, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321091, Length=465, Percent_Identity=23.6559139784946, Blast_Score=112, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6325166, Length=451, Percent_Identity=21.9512195121951, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6325240, Length=475, Percent_Identity=22.3157894736842, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=25.8205689277899, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17737741, Length=478, Percent_Identity=20.9205020920502, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR002218
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999 [H]

Pfam domain/function: PF01134 GIDA; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 45612; Mature: 45612

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNIMKVVIIGAGPAGVYSALTLSKHAKVTLIEREEKLGGTCVLYGCIPTKSILSQLII
CCCCEEEEEEEECCCHHHHHHHHHCCCCEEEEEECHHHCCCEEEEEECCCHHHHHHHHHH
SRQASNMSLNTLREYALTSINTISKSLEHLLNSHGIEVIHANGFLRSSMVHASNTSLAAD
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHEEECCCCCHHHH
KVLVSTGTRRERLGKVKFTEDLAYTNEDYNKVVIVGGDAGGIELGWMMKKLGKEVHLIDK
HEEECCCCCHHHCCCCEEEHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEEECC
NDLLLQNIDRTLSEIVTNFLSQIGIKLYLGKKVSKIDETSVTLEDNQKISGDAVFVTFGR
CCHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHCCCCCEEEEECCCEECCCEEEEEECC
KPNIEGFEEIPHEKYIYVDEYLRTQIPNIYAAGDIIGTFTAHEAIYAGIIAAKNMLGEKR
CCCCCCHHHCCCCCEEEHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCHHH
EFIVEGIPKVIYIYPQIAYVGTTNGNCVTFNTLNLTRTIVERESEGFLKICERDNRVIGA
HHHHHCCCEEEEEEEEEEEEECCCCCEEEEEECHHHHHHHHCCCCCHHHHHCCCCCEEEE
VAFMPYAEDVISLISVLIRYQISLKDTIDLVMPHPSYLEAITEALNMLQSKG
EEECCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNNIMKVVIIGAGPAGVYSALTLSKHAKVTLIEREEKLGGTCVLYGCIPTKSILSQLII
CCCCEEEEEEEECCCHHHHHHHHHCCCCEEEEEECHHHCCCEEEEEECCCHHHHHHHHHH
SRQASNMSLNTLREYALTSINTISKSLEHLLNSHGIEVIHANGFLRSSMVHASNTSLAAD
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHEEECCCCCHHHH
KVLVSTGTRRERLGKVKFTEDLAYTNEDYNKVVIVGGDAGGIELGWMMKKLGKEVHLIDK
HEEECCCCCHHHCCCCEEEHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEEECC
NDLLLQNIDRTLSEIVTNFLSQIGIKLYLGKKVSKIDETSVTLEDNQKISGDAVFVTFGR
CCHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHCCCCCEEEEECCCEECCCEEEEEECC
KPNIEGFEEIPHEKYIYVDEYLRTQIPNIYAAGDIIGTFTAHEAIYAGIIAAKNMLGEKR
CCCCCCHHHCCCCCEEEHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCHHH
EFIVEGIPKVIYIYPQIAYVGTTNGNCVTFNTLNLTRTIVERESEGFLKICERDNRVIGA
HHHHHCCCEEEEEEEEEEEEECCCCCEEEEEECHHHHHHHHCCCCCHHHHHCCCCCEEEE
VAFMPYAEDVISLISVLIRYQISLKDTIDLVMPHPSYLEAITEALNMLQSKG
EEECCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]