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The map label for this gene is garR [C]

Identifier: 15897852

GI number: 15897852

Start: 832542

End: 833411

Strand: Direct

Name: garR [C]

Synonym: SSO0974

Alternate gene names: 15897852

Gene position: 832542-833411 (Clockwise)

Preceding gene: 15897851

Following gene: 15897853

Centisome position: 27.82

GC content: 34.71

Gene sequence:

>870_bases
ATGAAGATAGGTCTTATAGGCCTAGGTATAATGGGATATAGAATAGCAGCCAATCTAGCTAAGGCTAATAAACTTAATTT
AGTGTATGATAGGACTCAGGAAAAAATTGAAAGTTTCGTTAAGGAATATAAGGTTAACGGTGTACAAAATACTAAAGATC
TTGTAGAATCATCAGATGTCATAATAACTATGTTGGCTGATGACTACGCAGTGAAGTCTGTGGTTGAACCATTAATACCA
TTGATGAAAGGCAAAATTTTAATTGATATGAGCACTATAAGTCCGACATTAAGTATTAGCTTAGCCAAGAGGATAGAAAG
CAATGGCGGTACAATGTTTGATGCTCCTGTAATAGGAACTTCAGTATTTGTTGAGCAAAAGAAATTGGTTGTATTAGTAG
GCGGACCTAAGGATAAGTTTGACATAGTTAATGATATAGCTAAGGAAACAGCATCGTTAGTAGTATATATGGGCCCTAAT
GGTATGGGACTTTATTCCAAGCTAGTAAATAACTTGCTTTTAGCCTCTTATGTCGCAGCTATAGCTGAAGCCTATAATTT
CGGAATAAGAGCTGGATTAGAGCCTCAACAAGTTGTTAATATATTAACTACATTAAGTAGTGCTAGGTCTCCAACTACTG
AATTAAAAGCACCTAAATTATTGAAAGAGGACTATTCAACTCAGTTTGCAACAAAACACATGAGGAAAGATTTAGAAATT
ATTATGAGGGAAGCTCAGAATTTAAAGATAATTACACCGGTTTCATCATTAGTATTAGAACTGTATAAAATGGCAGAAGC
ATTAGGATATTCTGAGGCCGATTTCATCTCAGTTGTAGAAGTCTTCAAGAAACTCTCACCTAAAACCTAA

Upstream 100 bases:

>100_bases
TAGTACTATCTGTTGATCTAATTGACGTTAAGGGGAGAATCAAAGATATAATTGAAAATTATCTAAAATCTTTTTAGTTT
GGAAGAATATATGCTAGTTT

Downstream 100 bases:

>100_bases
AAATCTCCTTTTTATACCTAAATTATGAACATCAATATTTCTGGTAAAAGAGTCCTCGTTACAGCATCCACAGAAGGCAT
TGGTAGGGGGATTGCAGAGG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MKIGLIGLGIMGYRIAANLAKANKLNLVYDRTQEKIESFVKEYKVNGVQNTKDLVESSDVIITMLADDYAVKSVVEPLIP
LMKGKILIDMSTISPTLSISLAKRIESNGGTMFDAPVIGTSVFVEQKKLVVLVGGPKDKFDIVNDIAKETASLVVYMGPN
GMGLYSKLVNNLLLASYVAAIAEAYNFGIRAGLEPQQVVNILTTLSSARSPTTELKAPKLLKEDYSTQFATKHMRKDLEI
IMREAQNLKIITPVSSLVLELYKMAEALGYSEADFISVVEVFKKLSPKT

Sequences:

>Translated_289_residues
MKIGLIGLGIMGYRIAANLAKANKLNLVYDRTQEKIESFVKEYKVNGVQNTKDLVESSDVIITMLADDYAVKSVVEPLIP
LMKGKILIDMSTISPTLSISLAKRIESNGGTMFDAPVIGTSVFVEQKKLVVLVGGPKDKFDIVNDIAKETASLVVYMGPN
GMGLYSKLVNNLLLASYVAAIAEAYNFGIRAGLEPQQVVNILTTLSSARSPTTELKAPKLLKEDYSTQFATKHMRKDLEI
IMREAQNLKIITPVSSLVLELYKMAEALGYSEADFISVVEVFKKLSPKT
>Mature_289_residues
MKIGLIGLGIMGYRIAANLAKANKLNLVYDRTQEKIESFVKEYKVNGVQNTKDLVESSDVIITMLADDYAVKSVVEPLIP
LMKGKILIDMSTISPTLSISLAKRIESNGGTMFDAPVIGTSVFVEQKKLVVLVGGPKDKFDIVNDIAKETASLVVYMGPN
GMGLYSKLVNNLLLASYVAAIAEAYNFGIRAGLEPQQVVNILTTLSSARSPTTELKAPKLLKEDYSTQFATKHMRKDLEI
IMREAQNLKIITPVSSLVLELYKMAEALGYSEADFISVVEVFKKLSPKT

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=293, Percent_Identity=34.4709897610922, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI40556376, Length=287, Percent_Identity=28.2229965156794, Blast_Score=111, Evalue=9e-25,
Organism=Escherichia coli, GI145693186, Length=293, Percent_Identity=32.4232081911263, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1786719, Length=287, Percent_Identity=28.5714285714286, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI1790315, Length=292, Percent_Identity=28.4246575342466, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1789092, Length=292, Percent_Identity=25.3424657534247, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17557316, Length=297, Percent_Identity=29.96632996633, Blast_Score=103, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24655230, Length=291, Percent_Identity=30.2405498281787, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI19922568, Length=291, Percent_Identity=30.2405498281787, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI28574115, Length=282, Percent_Identity=27.6595744680851, Blast_Score=114, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31659; Mature: 31659

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGLIGLGIMGYRIAANLAKANKLNLVYDRTQEKIESFVKEYKVNGVQNTKDLVESSDV
CEEEEEEHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCE
IITMLADDYAVKSVVEPLIPLMKGKILIDMSTISPTLSISLAKRIESNGGTMFDAPVIGT
EEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEECCHHCH
SVFVEQKKLVVLVGGPKDKFDIVNDIAKETASLVVYMGPNGMGLYSKLVNNLLLASYVAA
HHEEECCEEEEEECCCCCHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHH
IAEAYNFGIRAGLEPQQVVNILTTLSSARSPTTELKAPKLLKEDYSTQFATKHMRKDLEI
HHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHH
IMREAQNLKIITPVSSLVLELYKMAEALGYSEADFISVVEVFKKLSPKT
HHHHHCCCEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIGLIGLGIMGYRIAANLAKANKLNLVYDRTQEKIESFVKEYKVNGVQNTKDLVESSDV
CEEEEEEHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCE
IITMLADDYAVKSVVEPLIPLMKGKILIDMSTISPTLSISLAKRIESNGGTMFDAPVIGT
EEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEECCHHCH
SVFVEQKKLVVLVGGPKDKFDIVNDIAKETASLVVYMGPNGMGLYSKLVNNLLLASYVAA
HHEEECCEEEEEECCCCCHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHH
IAEAYNFGIRAGLEPQQVVNILTTLSSARSPTTELKAPKLLKEDYSTQFATKHMRKDLEI
HHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHH
IMREAQNLKIITPVSSLVLELYKMAEALGYSEADFISVVEVFKKLSPKT
HHHHHCCCEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA