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The map label for this gene is trm1

Identifier: 15897824

GI number: 15897824

Start: 800987

End: 802123

Strand: Direct

Name: trm1

Synonym: SSO0943

Alternate gene names: 15897824

Gene position: 800987-802123 (Clockwise)

Preceding gene: 15897823

Following gene: 15897827

Centisome position: 26.77

GC content: 34.04

Gene sequence:

>1137_bases
ATGAAATTAAAGGAAGTAACGGAAGGCAAGGTTAGAATATTCGTACCAGATCCTACAGGATATATGGTAGAAGGAAAATT
TGATCCATCATGGGCTCCAGTATTCTATAATCCTAAAATGACTTTCAATAGAGATCTCAGCGTAATTGTGGTTAGCATAT
TAAAACCTAAGATAATAGTAGACGCGTTAAGCGCAACGGGCATAAGAGGAATTAGATACTACGTTGAATCGTGGAAAAGC
GAACAACTAATTCTAAATGATAAAAATCCAAATGCCACTTCTTTAATTCAAATAAATGCGAAAAATAATGGGATTGAAAA
TGCTAAAATTTATAATAAAGACGCGAACGCTTTACTCTACGAAGTAAAAAGTGACTACATCGATATAGATCCTTTTGGTT
CTCCTGCACCTTTTATTCTTTCATCCTTAAACGCCGCAACAAGAAATGGAATCGTAGCATTTACAGCAACTGACCTATCT
CCGCTTGAGGGATCCTCACCAACATCATGTAGACGAAAATATGACGCAATTAATCACAAACTTTCCTCATCCAAGGAACT
TGGATTAAGAGTACTTATTGGCAAGATAATTAGAGAAGCCGCGATATTAGAAAAGACAGTATATCCGCTATTCTCTTTTT
ATGCAGATTATTATTATAGACTATTTGTAAGAGTGGAGAATGGTGCTAGAAAGGCAGACGATAATATTAATAAGCATCTT
AAATATTTTGGAGAATGCCCCCGCTGTGGATTTCAGACATTCGTAGAGGAGAATTGTAAGACTAAGTGCCCCGTATGTGG
CGAGATTTTTAGTATTATTGGTCCTTTGTATATTGGTCCTCTTTATAGTATGGAGTTTTTAAAAAGAATAATGGATTTAT
ATAGTAATTTTAACTACTTGACCTCTTTTAATAGAATACAAAAGTTACTTAATGTAATAGAAAAGGAGGCAAGATTTAAA
AATGTATTCTATAATATCAGTAAGCTCGCATCCAAATTAAAAATTTCAGCTATTCCTCCTATTGAATCTATTTTAGAATG
TCTAGGTGATGCTTCCAGAACTCATTTTGCTCCTACTGGGATAAGGACCGATAAGGAATATGAAGAAATAACAAAGTGTA
TTAAGAGCTTAAGATAA

Upstream 100 bases:

>100_bases
TCTGAATTAGTAATAATCATAAGGACTTCAAAGATGTTAAAAGAATGTGAAGATCTAGGATATAAAACTTATGAAGAAAA
TGATATACATTCTTTAATAG

Downstream 100 bases:

>100_bases
AGCTTTTTCTATGATCCAGCCAGATAATTTACTGCAAGTTTGTGGACTTACATGTATTCCATTTCCACATTTGGTTAAGC
TTTTACAACTTAAGCAAGGA

Product: N(2),N(2)-dimethylguanosine tRNA methyltransferase

Products: NA

Alternate protein names: tRNA 2,2-dimethylguanosine-26 methyltransferase; tRNA(guanine-26,N(2)-N(2)) methyltransferase; tRNA(m(2,2)G26)dimethyltransferase

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKLKEVTEGKVRIFVPDPTGYMVEGKFDPSWAPVFYNPKMTFNRDLSVIVVSILKPKIIVDALSATGIRGIRYYVESWKS
EQLILNDKNPNATSLIQINAKNNGIENAKIYNKDANALLYEVKSDYIDIDPFGSPAPFILSSLNAATRNGIVAFTATDLS
PLEGSSPTSCRRKYDAINHKLSSSKELGLRVLIGKIIREAAILEKTVYPLFSFYADYYYRLFVRVENGARKADDNINKHL
KYFGECPRCGFQTFVEENCKTKCPVCGEIFSIIGPLYIGPLYSMEFLKRIMDLYSNFNYLTSFNRIQKLLNVIEKEARFK
NVFYNISKLASKLKISAIPPIESILECLGDASRTHFAPTGIRTDKEYEEITKCIKSLR

Sequences:

>Translated_378_residues
MKLKEVTEGKVRIFVPDPTGYMVEGKFDPSWAPVFYNPKMTFNRDLSVIVVSILKPKIIVDALSATGIRGIRYYVESWKS
EQLILNDKNPNATSLIQINAKNNGIENAKIYNKDANALLYEVKSDYIDIDPFGSPAPFILSSLNAATRNGIVAFTATDLS
PLEGSSPTSCRRKYDAINHKLSSSKELGLRVLIGKIIREAAILEKTVYPLFSFYADYYYRLFVRVENGARKADDNINKHL
KYFGECPRCGFQTFVEENCKTKCPVCGEIFSIIGPLYIGPLYSMEFLKRIMDLYSNFNYLTSFNRIQKLLNVIEKEARFK
NVFYNISKLASKLKISAIPPIESILECLGDASRTHFAPTGIRTDKEYEEITKCIKSLR
>Mature_378_residues
MKLKEVTEGKVRIFVPDPTGYMVEGKFDPSWAPVFYNPKMTFNRDLSVIVVSILKPKIIVDALSATGIRGIRYYVESWKS
EQLILNDKNPNATSLIQINAKNNGIENAKIYNKDANALLYEVKSDYIDIDPFGSPAPFILSSLNAATRNGIVAFTATDLS
PLEGSSPTSCRRKYDAINHKLSSSKELGLRVLIGKIIREAAILEKTVYPLFSFYADYYYRLFVRVENGARKADDNINKHL
KYFGECPRCGFQTFVEENCKTKCPVCGEIFSIIGPLYIGPLYSMEFLKRIMDLYSNFNYLTSFNRIQKLLNVIEKEARFK
NVFYNISKLASKLKISAIPPIESILECLGDASRTHFAPTGIRTDKEYEEITKCIKSLR

Specific function: Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups

COG id: COG1867

COG function: function code J; N2,N2-dimethylguanosine tRNA methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TRM1 family

Homologues:

Organism=Homo sapiens, GI8923219, Length=336, Percent_Identity=27.0833333333333, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI209862871, Length=336, Percent_Identity=27.0833333333333, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI216548445, Length=317, Percent_Identity=27.4447949526814, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI163792194, Length=324, Percent_Identity=27.7777777777778, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17564890, Length=400, Percent_Identity=27.75, Blast_Score=142, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6320325, Length=352, Percent_Identity=28.6931818181818, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI19921190, Length=443, Percent_Identity=24.3792325056433, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRM1_SULSO (Q97ZH0)

Other databases:

- EMBL:   AE006641
- PIR:   D90245
- RefSeq:   NP_342429.1
- ProteinModelPortal:   Q97ZH0
- SMR:   Q97ZH0
- GeneID:   1455186
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0943
- NMPDR:   fig|273057.1.peg.852
- HOGENOM:   HBG577872
- OMA:   DAPVFYN
- PhylomeDB:   Q97ZH0
- ProtClustDB:   PRK04338
- BioCyc:   SSOL273057:SSO0943-MONOMER
- BRENDA:   2.1.1.32
- HAMAP:   MF_00290
- InterPro:   IPR022923
- InterPro:   IPR002905
- PANTHER:   PTHR10631
- TIGRFAMs:   TIGR00308

Pfam domain/function: PF02005 TRM

EC number: =2.1.1.32

Molecular weight: Translated: 42874; Mature: 42874

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKEVTEGKVRIFVPDPTGYMVEGKFDPSWAPVFYNPKMTFNRDLSVIVVSILKPKIIV
CCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCEEECCCCCCCCCHHHHHHHHHCCHHHH
DALSATGIRGIRYYVESWKSEQLILNDKNPNATSLIQINAKNNGIENAKIYNKDANALLY
HHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCCCCCEEECCCCCCEEE
EVKSDYIDIDPFGSPAPFILSSLNAATRNGIVAFTATDLSPLEGSSPTSCRRKYDAINHK
EECCCEEECCCCCCCHHHHHHHHCHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
LSSSKELGLRVLIGKIIREAAILEKTVYPLFSFYADYYYRLFVRVENGARKADDNINKHL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHH
KYFGECPRCGFQTFVEENCKTKCPVCGEIFSIIGPLYIGPLYSMEFLKRIMDLYSNFNYL
HHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
TSFNRIQKLLNVIEKEARFKNVFYNISKLASKLKISAIPPIESILECLGDASRTHFAPTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCC
IRTDKEYEEITKCIKSLR
CCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLKEVTEGKVRIFVPDPTGYMVEGKFDPSWAPVFYNPKMTFNRDLSVIVVSILKPKIIV
CCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCEEECCCCCCCCCHHHHHHHHHCCHHHH
DALSATGIRGIRYYVESWKSEQLILNDKNPNATSLIQINAKNNGIENAKIYNKDANALLY
HHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCCCCCEEECCCCCCEEE
EVKSDYIDIDPFGSPAPFILSSLNAATRNGIVAFTATDLSPLEGSSPTSCRRKYDAINHK
EECCCEEECCCCCCCHHHHHHHHCHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
LSSSKELGLRVLIGKIIREAAILEKTVYPLFSFYADYYYRLFVRVENGARKADDNINKHL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHH
KYFGECPRCGFQTFVEENCKTKCPVCGEIFSIIGPLYIGPLYSMEFLKRIMDLYSNFNYL
HHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
TSFNRIQKLLNVIEKEARFKNVFYNISKLASKLKISAIPPIESILECLGDASRTHFAPTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCC
IRTDKEYEEITKCIKSLR
CCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726