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The map label for this gene is trpB

Identifier: 15897776

GI number: 15897776

Start: 756108

End: 757385

Strand: Direct

Name: trpB

Synonym: SSO0888

Alternate gene names: 15897776

Gene position: 756108-757385 (Clockwise)

Preceding gene: 15897775

Following gene: 15897777

Centisome position: 25.27

GC content: 37.72

Gene sequence:

>1278_bases
ATGGTAAAAGAAGACGAGATTTTGCCTAAATATTGGTACAATATAATCCCTGATCTACCTAAACCCTTGCCTCCACCAAG
GGATCCACAAGGTGCCTATTTCTCGAGAATCGATTTATTAAGAAGTATACTACCCAAGGAGGTATTAAGACAACAATTCA
CAATAGAAAGGTATATAAAGATCCCTGAGGAAGTAAGAGATAGATATTTATCGATAGGAAGACCAACTCCATTATTTAGG
GCTAAAAGGTTAGAAGAGTACTTAAAGACACCAGCAAGAATTTACTTTAAATATGAAGGTGCTACACCTACTGGATCTCA
TAAGATAAATACAGCAATTCCTCAAGCGTATTTTGCAAAAGAAGAGGGAATTGAACACGTAGTTACTGAAACTGGAGCTG
GTCAATGGGGAACTGCAGTCGCACTTGCAGCTAGTATGTATAATATGAAAAGTACTATATTCATGGTAAAGGTAAGTTAT
GAACAAAAACCGATGAGAAGGAGTATAATGCAATTATATGGGGCTAATGTTTACGCAAGCCCCACAAACTTAACTGAATA
CGGTAGGAAGATATTAGAGACAAACCCACAGCATCCAGGATCATTAGGTATAGCAATGAGCGAGGCAATAGAGTATGCTC
TTAAGAACGAATTTAGATATTTAGTAGGTAGCGTTTTAGATGTAGTACTTTTGCATCAGAGTGTTATTGGTCAAGAGACT
ATTACTCAATTGGATTTGTTAGGAGAAGACGCTGATATCCTAATTGGATGTGTAGGAGGTGGGAGCAATTTTGGCGGTTT
CACATACCCCTTTATCGGAAATAAGAAAGGCAAGCGTTATATTGCAGTAAGTTCTGCAGAAATTCCAAAGTTTAGTAAAG
GTGAATATAAATACGATTTTCCAGACTCTGCTGGATTATTACCTTTAGTGAAAATGATAACTTTAGGTAAAGATTACGTT
CCGCCACCAATATACGCAGGCGGGTTAAGATATCATGGTGTAGCACCAACATTAAGTTTGTTAACAAAGGAGGGTATTGT
GGAATGGAGAGAATACAATGAAAGGGAGATTTTCGAAGCTGCTAAGATATTTATCGAGAACCAAGGTATTGTACCAGCCC
CAGAATCAGCTCATGCAATAAGGGCAGTAGTTGATGAAGCTATAGAGGCAAGAAAGAATAATGAGCGAAAGGTCATCGTC
TTTAATCTAAGTGGACATGGATTGTTAGATCTGTCAAATTACGAATCCATGATGAAAAGGTTGAATGGAAATGGGTAA

Upstream 100 bases:

>100_bases
CTCATTTAGCGAATATAAGGGAGAATTTGAGGATTTTGAAACATACTAAAAATTTATAAGGTAAAAACATTTATAGTGTA
TCGGTGCTTTTGATGTACCA

Downstream 100 bases:

>100_bases
GATGCTTGTAGTCTATATGACGTTAGGTTATCCAAATGTTCAAAGTTTTAAGGATTTTATTATTGGGGCAGTTGAAAATG
GAGCTGATATATTAGAGCTT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MVKEDEILPKYWYNIIPDLPKPLPPPRDPQGAYFSRIDLLRSILPKEVLRQQFTIERYIKIPEEVRDRYLSIGRPTPLFR
AKRLEEYLKTPARIYFKYEGATPTGSHKINTAIPQAYFAKEEGIEHVVTETGAGQWGTAVALAASMYNMKSTIFMVKVSY
EQKPMRRSIMQLYGANVYASPTNLTEYGRKILETNPQHPGSLGIAMSEAIEYALKNEFRYLVGSVLDVVLLHQSVIGQET
ITQLDLLGEDADILIGCVGGGSNFGGFTYPFIGNKKGKRYIAVSSAEIPKFSKGEYKYDFPDSAGLLPLVKMITLGKDYV
PPPIYAGGLRYHGVAPTLSLLTKEGIVEWREYNEREIFEAAKIFIENQGIVPAPESAHAIRAVVDEAIEARKNNERKVIV
FNLSGHGLLDLSNYESMMKRLNGNG

Sequences:

>Translated_425_residues
MVKEDEILPKYWYNIIPDLPKPLPPPRDPQGAYFSRIDLLRSILPKEVLRQQFTIERYIKIPEEVRDRYLSIGRPTPLFR
AKRLEEYLKTPARIYFKYEGATPTGSHKINTAIPQAYFAKEEGIEHVVTETGAGQWGTAVALAASMYNMKSTIFMVKVSY
EQKPMRRSIMQLYGANVYASPTNLTEYGRKILETNPQHPGSLGIAMSEAIEYALKNEFRYLVGSVLDVVLLHQSVIGQET
ITQLDLLGEDADILIGCVGGGSNFGGFTYPFIGNKKGKRYIAVSSAEIPKFSKGEYKYDFPDSAGLLPLVKMITLGKDYV
PPPIYAGGLRYHGVAPTLSLLTKEGIVEWREYNEREIFEAAKIFIENQGIVPAPESAHAIRAVVDEAIEARKNNERKVIV
FNLSGHGLLDLSNYESMMKRLNGNG
>Mature_425_residues
MVKEDEILPKYWYNIIPDLPKPLPPPRDPQGAYFSRIDLLRSILPKEVLRQQFTIERYIKIPEEVRDRYLSIGRPTPLFR
AKRLEEYLKTPARIYFKYEGATPTGSHKINTAIPQAYFAKEEGIEHVVTETGAGQWGTAVALAASMYNMKSTIFMVKVSY
EQKPMRRSIMQLYGANVYASPTNLTEYGRKILETNPQHPGSLGIAMSEAIEYALKNEFRYLVGSVLDVVLLHQSVIGQET
ITQLDLLGEDADILIGCVGGGSNFGGFTYPFIGNKKGKRYIAVSSAEIPKFSKGEYKYDFPDSAGLLPLVKMITLGKDYV
PPPIYAGGLRYHGVAPTLSLLTKEGIVEWREYNEREIFEAAKIFIENQGIVPAPESAHAIRAVVDEAIEARKNNERKVIV
FNLSGHGLLDLSNYESMMKRLNGNG

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=370, Percent_Identity=28.6486486486486, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321412, Length=383, Percent_Identity=30.2872062663185, Blast_Score=141, Evalue=3e-34,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB1_SULSO (P50383)

Other databases:

- EMBL:   Z50014
- EMBL:   AE006641
- PIR:   D90239
- RefSeq:   NP_342381.1
- ProteinModelPortal:   P50383
- GeneID:   1455138
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0888
- NMPDR:   fig|273057.1.peg.804
- HOGENOM:   HBG303148
- OMA:   EAYINIG
- ProtClustDB:   PRK12391
- BioCyc:   SSOL273057:SSO0888-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026
- TIGRFAMs:   TIGR01415

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 47708; Mature: 47708

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKEDEILPKYWYNIIPDLPKPLPPPRDPQGAYFSRIDLLRSILPKEVLRQQFTIERYIK
CCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
IPEEVRDRYLSIGRPTPLFRAKRLEEYLKTPARIYFKYEGATPTGSHKINTAIPQAYFAK
CCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHCCHHHHHH
EEGIEHVVTETGAGQWGTAVALAASMYNMKSTIFMVKVSYEQKPMRRSIMQLYGANVYAS
HHCHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEC
PTNLTEYGRKILETNPQHPGSLGIAMSEAIEYALKNEFRYLVGSVLDVVLLHQSVIGQET
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITQLDLLGEDADILIGCVGGGSNFGGFTYPFIGNKKGKRYIAVSSAEIPKFSKGEYKYDF
HHHHHHCCCCCCEEEEEECCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCCCCEEECC
PDSAGLLPLVKMITLGKDYVPPPIYAGGLRYHGVAPTLSLLTKEGIVEWREYNEREIFEA
CCCCCHHHHHHHHHHCCCCCCCCCEECCEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHH
AKIFIENQGIVPAPESAHAIRAVVDEAIEARKNNERKVIVFNLSGHGLLDLSNYESMMKR
HHHHEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEHHHHHHHHHH
LNGNG
HCCCC
>Mature Secondary Structure
MVKEDEILPKYWYNIIPDLPKPLPPPRDPQGAYFSRIDLLRSILPKEVLRQQFTIERYIK
CCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
IPEEVRDRYLSIGRPTPLFRAKRLEEYLKTPARIYFKYEGATPTGSHKINTAIPQAYFAK
CCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHCCHHHHHH
EEGIEHVVTETGAGQWGTAVALAASMYNMKSTIFMVKVSYEQKPMRRSIMQLYGANVYAS
HHCHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEC
PTNLTEYGRKILETNPQHPGSLGIAMSEAIEYALKNEFRYLVGSVLDVVLLHQSVIGQET
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITQLDLLGEDADILIGCVGGGSNFGGFTYPFIGNKKGKRYIAVSSAEIPKFSKGEYKYDF
HHHHHHCCCCCCEEEEEECCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCCCCEEECC
PDSAGLLPLVKMITLGKDYVPPPIYAGGLRYHGVAPTLSLLTKEGIVEWREYNEREIFEA
CCCCCHHHHHHHHHHCCCCCCCCCEECCEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHH
AKIFIENQGIVPAPESAHAIRAVVDEAIEARKNNERKVIVFNLSGHGLLDLSNYESMMKR
HHHHEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEHHHHHHHHHH
LNGNG
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726