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The map label for this gene is bioR [H]

Identifier: 15897572

GI number: 15897572

Start: 572192

End: 572893

Strand: Reverse

Name: bioR [H]

Synonym: SSO0662

Alternate gene names: 15897572

Gene position: 572893-572192 (Counterclockwise)

Preceding gene: 15897573

Following gene: 15897569

Centisome position: 19.15

GC content: 34.33

Gene sequence:

>702_bases
ATGTGTGGAAGTATGTTGGTAATAAGACTCAATAAGGTTACATCTACACAAGATTTCGCAGAAGCAATTAGCAATATGTT
GTTTGAAGATTTTCTGGTAATCGCAGAGGAGCAAACTAAAGCTAGGGGAAGATTAGGGAGAAGTTGGTATTCTCCCAAAG
GTGGACTTTGGTTTACTTATGTAAAAAAGAACTTTAAAACAGAAGAGATTCAGTTATCTAGCCTAAAAGTTGCCGTTGCA
GTTCTAGATGTTTTAAGAAAGTTTGTAGATGCAAAGATAAGATGGCCTAATGATATAGTGGTTAATGATAAGAAAATTGC
AGGGATTTTGATTGAAGCTAAGACTTATGAAGAGGACATTAAGTTAGCGGATCTCTTTATAGGTGCTGGTATAGATATTC
TCATCAAAGAATTCCCTAGTGATCTAAACGCTACATCATTATATTTAGAGATAGGTAAGGAAGTAGAACTCAATATAGAT
GAGGTGATAGGCAAAATAGAGGAGCACTTAAAGTTAGATAATAGAAAAATTATTGACATAGTGAATCAGTATCTCTCAAT
AAAGGATAGAGAAGTAACGCTAAAAGGAGAAAATTGGGAAAAGAGATGTAAGGCACTATTCGTAGATTATATGGGAAGGC
TAGTGACAGAATGTGGAATATATGAGGTTGAAGAGATTTATAGGGTTGAAGTTTCACATTAA

Upstream 100 bases:

>100_bases
ATTGGAGTTATTCATAGTATTAGTGAGAAAGGAATAGGATTGGCAATAATGAATAGGACAAGAAAGGCATCCGGTTTCTC
TTCAATTTATAATATAGAAG

Downstream 100 bases:

>100_bases
TAATGAAAGACCTCTTACCTTTAATTTTCCACTGACTACTTTTCCATAGTTCTCAATATTTTTGGAAGTACTCACAATAT
TTTTGAGCAACTGCTTAAGA

Product: biotin--(acetyl-CoA carboxylase) synthetase (bioR)

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MCGSMLVIRLNKVTSTQDFAEAISNMLFEDFLVIAEEQTKARGRLGRSWYSPKGGLWFTYVKKNFKTEEIQLSSLKVAVA
VLDVLRKFVDAKIRWPNDIVVNDKKIAGILIEAKTYEEDIKLADLFIGAGIDILIKEFPSDLNATSLYLEIGKEVELNID
EVIGKIEEHLKLDNRKIIDIVNQYLSIKDREVTLKGENWEKRCKALFVDYMGRLVTECGIYEVEEIYRVEVSH

Sequences:

>Translated_233_residues
MCGSMLVIRLNKVTSTQDFAEAISNMLFEDFLVIAEEQTKARGRLGRSWYSPKGGLWFTYVKKNFKTEEIQLSSLKVAVA
VLDVLRKFVDAKIRWPNDIVVNDKKIAGILIEAKTYEEDIKLADLFIGAGIDILIKEFPSDLNATSLYLEIGKEVELNID
EVIGKIEEHLKLDNRKIIDIVNQYLSIKDREVTLKGENWEKRCKALFVDYMGRLVTECGIYEVEEIYRVEVSH
>Mature_233_residues
MCGSMLVIRLNKVTSTQDFAEAISNMLFEDFLVIAEEQTKARGRLGRSWYSPKGGLWFTYVKKNFKTEEIQLSSLKVAVA
VLDVLRKFVDAKIRWPNDIVVNDKKIAGILIEAKTYEEDIKLADLFIGAGIDILIKEFPSDLNATSLYLEIGKEVELNID
EVIGKIEEHLKLDNRKIIDIVNQYLSIKDREVTLKGENWEKRCKALFVDYMGRLVTECGIYEVEEIYRVEVSH

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=109, Percent_Identity=37.6146788990826, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 26757; Mature: 26757

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGSMLVIRLNKVTSTQDFAEAISNMLFEDFLVIAEEQTKARGRLGRSWYSPKGGLWFTY
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
VKKNFKTEEIQLSSLKVAVAVLDVLRKFVDAKIRWPNDIVVNDKKIAGILIEAKTYEEDI
ECCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEEEEEEEECCCHHHH
KLADLFIGAGIDILIKEFPSDLNATSLYLEIGKEVELNIDEVIGKIEEHLKLDNRKIIDI
HHHHHHHHCCHHHHHHHCCCCCCCEEEEEEECCCEECCHHHHHHHHHHHHHCCCCHHHHH
VNQYLSIKDREVTLKGENWEKRCKALFVDYMGRLVTECGIYEVEEIYRVEVSH
HHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEECC
>Mature Secondary Structure
MCGSMLVIRLNKVTSTQDFAEAISNMLFEDFLVIAEEQTKARGRLGRSWYSPKGGLWFTY
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
VKKNFKTEEIQLSSLKVAVAVLDVLRKFVDAKIRWPNDIVVNDKKIAGILIEAKTYEEDI
ECCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEEEEEEEECCCHHHH
KLADLFIGAGIDILIKEFPSDLNATSLYLEIGKEVELNIDEVIGKIEEHLKLDNRKIIDI
HHHHHHHHCCHHHHHHHCCCCCCCEEEEEEECCCEECCHHHHHHHHHHHHHCCCCHHHHH
VNQYLSIKDREVTLKGENWEKRCKALFVDYMGRLVTECGIYEVEEIYRVEVSH
HHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]