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The map label for this gene is priB

Identifier: 15897479

GI number: 15897479

Start: 490710

End: 491633

Strand: Reverse

Name: priB

Synonym: SSO0557

Alternate gene names: 15897479

Gene position: 491633-490710 (Counterclockwise)

Preceding gene: 15897481

Following gene: 15897477

Centisome position: 16.43

GC content: 32.9

Gene sequence:

>924_bases
ATGGCATTAGACGTTAAAAAGTATCCTTTTATAAAGAGTCTTGATGATGAACTAAAGAAATATGGAGGAGGAATTACATT
AACAGATTTACTATTAAATAGCACTACCCTCATTGATCAAGCTAAAGATAGGATACAAAAGACTAAATCTGGTGACGAAC
TTCCACATTACGTCTCTTATAATGAACCCGTACTCGTGTTTTACACTACGCTACTTTCTCTAGCCATATTAAATGATGTG
AAGCTTATTAGAAGATACGCATATGCAGAGGCTAAACAATTCAGATCCCTACTTCACACCGAGAATGAGGAGAATTTACT
TGAAATCTCTAAGTTATTAGATCTGAAGATAAATAGATGTGATCCTATAAAATTCTATTTAGAGAAAAAGAGAAGGATAA
TACAAAAAGAATTTTGTGTTCATTTTATCGATTATCTCAAATATACAAAAGACCTTAAGGAAGATTGGAAACTAAGTGGG
CAGATCTTACATAAGGGATACGTATATCTGGACAAGAATCAACTAATTGGCCTAATAGCTGAAAGTATTAAGAGTAAGAT
AGTCGAAATGATAAGACCACTGAATCTTAAAGAAATACCAGAAAAATTGAAGAGCTTGATAGAAAGGAGAGGAATAATTC
CTCCATGCATAGAAAATATTTTAGCTAAGGAAAAATTAAACGAAGAAGAAATAAGGACTTTAATTACGTTTTACATTGAC
ATAGGTAAGGGACTAAGTGGTATAGTATCTATTATGAAAAAATATAACGTTTCAAACGTCGAGGACTTATATAGGAAATA
TAGGGGAGATAAGGGGACGAGGTATATCGTTTATTCTTGTGCTAAGATGAAGCAACTAGGTTTATGTGTGAGCAGTTGCA
ACGTTAAAAATCCCTTACAGCTCTACTTCCTAAGTAACGAATAG

Upstream 100 bases:

>100_bases
ATTTACATATGGCCAGGCGGCTGTAACTAGCACTTTCATTATATTTTGTGTTTAGAGCAAGTCTTTATTAAACATAACTA
AACATACTTTAAGTAAGGAC

Downstream 100 bases:

>100_bases
ACAGCGTAAAACCTCTGGATTACCGTGATCGCTGTGAGTAACATAAATAGGTAAAATAGATACAAGGAAACTTGTTTGCT
AACTATTATATAAAGTAATA

Product: DNA primase large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MALDVKKYPFIKSLDDELKKYGGGITLTDLLLNSTTLIDQAKDRIQKTKSGDELPHYVSYNEPVLVFYTTLLSLAILNDV
KLIRRYAYAEAKQFRSLLHTENEENLLEISKLLDLKINRCDPIKFYLEKKRRIIQKEFCVHFIDYLKYTKDLKEDWKLSG
QILHKGYVYLDKNQLIGLIAESIKSKIVEMIRPLNLKEIPEKLKSLIERRGIIPPCIENILAKEKLNEEEIRTLITFYID
IGKGLSGIVSIMKKYNVSNVEDLYRKYRGDKGTRYIVYSCAKMKQLGLCVSSCNVKNPLQLYFLSNE

Sequences:

>Translated_307_residues
MALDVKKYPFIKSLDDELKKYGGGITLTDLLLNSTTLIDQAKDRIQKTKSGDELPHYVSYNEPVLVFYTTLLSLAILNDV
KLIRRYAYAEAKQFRSLLHTENEENLLEISKLLDLKINRCDPIKFYLEKKRRIIQKEFCVHFIDYLKYTKDLKEDWKLSG
QILHKGYVYLDKNQLIGLIAESIKSKIVEMIRPLNLKEIPEKLKSLIERRGIIPPCIENILAKEKLNEEEIRTLITFYID
IGKGLSGIVSIMKKYNVSNVEDLYRKYRGDKGTRYIVYSCAKMKQLGLCVSSCNVKNPLQLYFLSNE
>Mature_306_residues
ALDVKKYPFIKSLDDELKKYGGGITLTDLLLNSTTLIDQAKDRIQKTKSGDELPHYVSYNEPVLVFYTTLLSLAILNDVK
LIRRYAYAEAKQFRSLLHTENEENLLEISKLLDLKINRCDPIKFYLEKKRRIIQKEFCVHFIDYLKYTKDLKEDWKLSGQ
ILHKGYVYLDKNQLIGLIAESIKSKIVEMIRPLNLKEIPEKLKSLIERRGIIPPCIENILAKEKLNEEEIRTLITFYIDI
GKGLSGIVSIMKKYNVSNVEDLYRKYRGDKGTRYIVYSCAKMKQLGLCVSSCNVKNPLQLYFLSNE

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG2219

COG function: function code L; Eukaryotic-type DNA primase, large subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic-type primase large subunit family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIL_SULSO (Q9UWW1)

Other databases:

- EMBL:   Y18930
- EMBL:   AE006641
- PIR:   C90202
- RefSeq:   NP_342084.1
- PDB:   1ZT2
- PDBsum:   1ZT2
- ProteinModelPortal:   Q9UWW1
- SMR:   Q9UWW1
- MINT:   MINT-1896335
- GeneID:   1454841
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0557
- NMPDR:   fig|273057.1.peg.507
- HOGENOM:   HBG704862
- OMA:   GRKTPPC
- ProtClustDB:   PRK09568
- BioCyc:   SSOL273057:SSO0557-MONOMER
- HAMAP:   MF_00701
- InterPro:   IPR007238

Pfam domain/function: PF04104 DNA_primase_lrg

EC number: NA

Molecular weight: Translated: 35734; Mature: 35603

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDVKKYPFIKSLDDELKKYGGGITLTDLLLNSTTLIDQAKDRIQKTKSGDELPHYVSY
CCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
NEPVLVFYTTLLSLAILNDVKLIRRYAYAEAKQFRSLLHTENEENLLEISKLLDLKINRC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
DPIKFYLEKKRRIIQKEFCVHFIDYLKYTKDLKEDWKLSGQILHKGYVYLDKNQLIGLIA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHH
ESIKSKIVEMIRPLNLKEIPEKLKSLIERRGIIPPCIENILAKEKLNEEEIRTLITFYID
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
IGKGLSGIVSIMKKYNVSNVEDLYRKYRGDKGTRYIVYSCAKMKQLGLCVSSCNVKNPLQ
HCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEE
LYFLSNE
EEEECCC
>Mature Secondary Structure 
ALDVKKYPFIKSLDDELKKYGGGITLTDLLLNSTTLIDQAKDRIQKTKSGDELPHYVSY
CCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
NEPVLVFYTTLLSLAILNDVKLIRRYAYAEAKQFRSLLHTENEENLLEISKLLDLKINRC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
DPIKFYLEKKRRIIQKEFCVHFIDYLKYTKDLKEDWKLSGQILHKGYVYLDKNQLIGLIA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHH
ESIKSKIVEMIRPLNLKEIPEKLKSLIERRGIIPPCIENILAKEKLNEEEIRTLITFYID
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
IGKGLSGIVSIMKKYNVSNVEDLYRKYRGDKGTRYIVYSCAKMKQLGLCVSSCNVKNPLQ
HCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEE
LYFLSNE
EEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10701121; 11427726