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The map label for this gene is tfb

Identifier: 15897377

GI number: 15897377

Start: 385794

End: 386723

Strand: Reverse

Name: tfb

Synonym: SSO0446

Alternate gene names: 15897377

Gene position: 386723-385794 (Counterclockwise)

Preceding gene: 15897380

Following gene: 15897375

Centisome position: 12.92

GC content: 39.35

Gene sequence:

>930_bases
GTGTTGTATTTGTCTGAAGAAAATAAATCCGTATCCACTCCTTGCCCTCCTGATAAGATTATCTTTGATGCAGAGAGGGG
GGAGTACATTTGCTCTGAAACTGGAGAAGTTTTAGAAGATAAAATTATAGATCAAGGGCCAGAGTGGAGGGCCTTCACGC
CAGAGGAGAAAGAAAAGAGAAGCAGAGTTGGAGGGCCTTTAAACAATACTATTCACGATAGGGGTTTATCCACTCTTATA
GACTGGAAAGATAAGGATGCTATGGGAAGAACTTTAGACCCTAAGAGAAGACTTGAGGCATTGAGATGGAGAAAGTGGCA
AATTAGAGCGAGAATTCAATCTTCCATAGATAGAAACTTAGCACAAGCTATGAATGAACTAGAAAGAATTGGGAATTTAC
TAAACTTACCAAAATCAGTTAAAGATGAGGCTGCATTAATCTATAGAAAAGCAGTAGAGAAAGGATTAGTGAGGGGAAGG
AGTATAGAAAGCGTCGTAGCAGCTGCAATATACGCTGCATGTAGAAGAATGAAGTTGGCCAGAACTTTGGATGAGATAGC
CCAATATACTAAGGCTAATAGAAAGGAAGTAGCGAGATGCTATAGACTATTACTTAGAGAACTAGATGTTAGTGTACCAG
TAAGTGATCCCAAGGATTACGTAACTAGAATAGCTAACTTGTTAGGTCTAAGCGGAGCTGTTATGAAAACGGCGGCTGAA
ATTATAGATAAGGCGAAAGGTTCTGGGTTAACTGCTGGTAAAGATCCAGCTGGTTTAGCTGCGGCTGCAATTTATATAGC
ATCATTACTACATGATGAAAGAAGAACGCAAAAAGAGATAGCTCAAGTTGCAGGTGTCACGGAAGTTACTGTGAGAAACA
GATATAAAGAGTTAACACAAGAGCTAAAAATATCAATACCTACTCAATAA

Upstream 100 bases:

>100_bases
TCATTTTTAGATAAATAATGGGGAACATGGGAGAAAGATTTATAAGTAAGGAAACAGAAAAGTTTAAAAGGTTTACTATT
CGTGGCTATATATAATTGGG

Downstream 100 bases:

>100_bases
CTAGAATATTGGATTCTGCATATCCCATTTTTATTAGGATCTCCCTAACTTTTTCTTTATGATCCCCTTGAATAAGTATC
TTTCCATCTTTTACTGTACC

Product: transcription initiation factor IIB

Products: NA

Alternate protein names: TFIIB 1

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MLYLSEENKSVSTPCPPDKIIFDAERGEYICSETGEVLEDKIIDQGPEWRAFTPEEKEKRSRVGGPLNNTIHDRGLSTLI
DWKDKDAMGRTLDPKRRLEALRWRKWQIRARIQSSIDRNLAQAMNELERIGNLLNLPKSVKDEAALIYRKAVEKGLVRGR
SIESVVAAAIYAACRRMKLARTLDEIAQYTKANRKEVARCYRLLLRELDVSVPVSDPKDYVTRIANLLGLSGAVMKTAAE
IIDKAKGSGLTAGKDPAGLAAAAIYIASLLHDERRTQKEIAQVAGVTEVTVRNRYKELTQELKISIPTQ

Sequences:

>Translated_309_residues
MLYLSEENKSVSTPCPPDKIIFDAERGEYICSETGEVLEDKIIDQGPEWRAFTPEEKEKRSRVGGPLNNTIHDRGLSTLI
DWKDKDAMGRTLDPKRRLEALRWRKWQIRARIQSSIDRNLAQAMNELERIGNLLNLPKSVKDEAALIYRKAVEKGLVRGR
SIESVVAAAIYAACRRMKLARTLDEIAQYTKANRKEVARCYRLLLRELDVSVPVSDPKDYVTRIANLLGLSGAVMKTAAE
IIDKAKGSGLTAGKDPAGLAAAAIYIASLLHDERRTQKEIAQVAGVTEVTVRNRYKELTQELKISIPTQ
>Mature_309_residues
MLYLSEENKSVSTPCPPDKIIFDAERGEYICSETGEVLEDKIIDQGPEWRAFTPEEKEKRSRVGGPLNNTIHDRGLSTLI
DWKDKDAMGRTLDPKRRLEALRWRKWQIRARIQSSIDRNLAQAMNELERIGNLLNLPKSVKDEAALIYRKAVEKGLVRGR
SIESVVAAAIYAACRRMKLARTLDEIAQYTKANRKEVARCYRLLLRELDVSVPVSDPKDYVTRIANLLGLSGAVMKTAAE
IIDKAKGSGLTAGKDPAGLAAAAIYIASLLHDERRTQKEIAQVAGVTEVTVRNRYKELTQELKISIPTQ

Specific function: Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre- initiation complex (DNA-TBP-TFIIB)

COG id: COG1405

COG function: function code K; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TFIIB family

Homologues:

Organism=Homo sapiens, GI4504193, Length=288, Percent_Identity=35.0694444444444, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI22035556, Length=287, Percent_Identity=28.9198606271777, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17564892, Length=292, Percent_Identity=31.8493150684932, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17531397, Length=289, Percent_Identity=24.5674740484429, Blast_Score=91, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6325343, Length=316, Percent_Identity=28.1645569620253, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6321685, Length=193, Percent_Identity=26.9430051813472, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17136758, Length=292, Percent_Identity=35.958904109589, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI281361961, Length=289, Percent_Identity=25.2595155709343, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24647658, Length=289, Percent_Identity=25.2595155709343, Blast_Score=88, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TF2B1_SULSO (P58111)

Other databases:

- EMBL:   AE006641
- PIR:   E90189
- RefSeq:   NP_341982.1
- ProteinModelPortal:   P58111
- GeneID:   1455582
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0446
- NMPDR:   fig|273057.1.peg.405
- HOGENOM:   HBG646536
- OMA:   HDYERAE
- PhylomeDB:   P58111
- ProtClustDB:   PRK00423
- BioCyc:   SSOL273057:SSO0446-MONOMER
- HAMAP:   MF_00383
- InterPro:   IPR006670
- InterPro:   IPR011028
- InterPro:   IPR013763
- InterPro:   IPR000812
- InterPro:   IPR013150
- InterPro:   IPR013137
- Gene3D:   G3DSA:1.10.472.10
- PANTHER:   PTHR11618
- PRINTS:   PR00685
- SMART:   SM00385

Pfam domain/function: PF00382 TFIIB; PF08271 TFIIB_Zn_Ribbon; SSF47954 Cyclin_like

EC number: NA

Molecular weight: Translated: 34671; Mature: 34671

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS00782 TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYLSEENKSVSTPCPPDKIIFDAERGEYICSETGEVLEDKIIDQGPEWRAFTPEEKEKR
CCEECCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
SRVGGPLNNTIHDRGLSTLIDWKDKDAMGRTLDPKRRLEALRWRKWQIRARIQSSIDRNL
HHCCCCCCCHHHHHCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQAMNELERIGNLLNLPKSVKDEAALIYRKAVEKGLVRGRSIESVVAAAIYAACRRMKLA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
RTLDEIAQYTKANRKEVARCYRLLLRELDVSVPVSDPKDYVTRIANLLGLSGAVMKTAAE
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
IIDKAKGSGLTAGKDPAGLAAAAIYIASLLHDERRTQKEIAQVAGVTEVTVRNRYKELTQ
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ELKISIPTQ
HHHHCCCCC
>Mature Secondary Structure
MLYLSEENKSVSTPCPPDKIIFDAERGEYICSETGEVLEDKIIDQGPEWRAFTPEEKEKR
CCEECCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
SRVGGPLNNTIHDRGLSTLIDWKDKDAMGRTLDPKRRLEALRWRKWQIRARIQSSIDRNL
HHCCCCCCCHHHHHCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQAMNELERIGNLLNLPKSVKDEAALIYRKAVEKGLVRGRSIESVVAAAIYAACRRMKLA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
RTLDEIAQYTKANRKEVARCYRLLLRELDVSVPVSDPKDYVTRIANLLGLSGAVMKTAAE
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
IIDKAKGSGLTAGKDPAGLAAAAIYIASLLHDERRTQKEIAQVAGVTEVTVRNRYKELTQ
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ELKISIPTQ
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726