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The map label for this gene is tkt-2 [C]

Identifier: 15897244

GI number: 15897244

Start: 256072

End: 257013

Strand: Direct

Name: tkt-2 [C]

Synonym: SSO0299

Alternate gene names: 15897244

Gene position: 256072-257013 (Clockwise)

Preceding gene: 15897243

Following gene: 15897245

Centisome position: 8.56

GC content: 38.54

Gene sequence:

>942_bases
ATGATGCAAGGAAATATTTACTCAATGCGTGAAACCTTCGGAAGGCTATTAGCAGACCTAGGGGATAAGAACAAGGATCT
AGTCGTGATAACTGCAGATGTAGGAGACTCTACCAGAGCGCTATACTTTAGAGAGAAGTTTAAGGATAGATACTTTAATG
TAGGCATAGCAGAGCAAGATATGGTGAATTTTGCTGCTGGCTTGGCTGCTGTAGGAAAAAAGCCCGCTATAGTTAACTTT
GGAATGTTCTTAATGAGAGCGTGGGAGCAGATAAGAAATAGTATAGCTAGAATGAATCTAGACGTCAAGATGTTTGTAAC
ACACACCGGATACAGTGACCACGGTGATGGTTCGAGTCATCAAGTTCTCGAAGATATAGCGCTAATGCGTGTATTACCAA
ACATGAAAGTAGTAGTACCAGCAGATCCTAAGGATATTGAAAGAAGCTTACCAGTTATAATTAATGAGGAAAGGGGTCCA
TTGTATTATAGGATAGGTAGAGAATATTCACCACCAATCACTATAGGACAAGAATACGAATTCAAGATTGGTAAAGCTTA
TGTGATTAAGGATGGGAGTGACTTAGCCATAATAGGAGCAGGCGTTGTTTTGTGGGATGCACTAAAGGCGGCTGAAGAAT
TAGAGAAATTAGGAATTAGCGTAGCAGTTATAAATTTATTCTCAATAAAGCCTATTGACGAAAATACAATAGAATATTAT
GCTAGAAAGGCTGGTAAGATAATTACTATTGAGGAACATAGCATATATGGAGGTATTGGTTCTGCCGTTGCAGAGGTTAC
GGCTAGGCGTTATCCAGTACCCATAAGATTTGTAGGTGCTACGACTTTTGGAAGATCTGCTAGAAGCCAAAGGGATCTAC
TAGATTACTATAATATAAACTATAAAACAATTATAAGGGAGGCAATTGATTTATTGAAGTAG

Upstream 100 bases:

>100_bases
GCATATAAGGCAAGCAAGCCCGTAGTAATATTCGCTAAGACTGTAAGAGGAAAGGGGTTTCCTCCAATAGAAAATACCCA
TAAACAGAGGTCCAGTCCAG

Downstream 100 bases:

>100_bases
ATGACTGAAGAAATAACACGGCTGAGGGAAGAAATAGATAAGGTAGACGAGCAGTTAGTAAAGTTACTCTCATATAGATT
AGAATTATCTAGAAAAATAG

Product: transketolase, C-terminal section (tkt-2)

Products: NA

Alternate protein names: TK [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MMQGNIYSMRETFGRLLADLGDKNKDLVVITADVGDSTRALYFREKFKDRYFNVGIAEQDMVNFAAGLAAVGKKPAIVNF
GMFLMRAWEQIRNSIARMNLDVKMFVTHTGYSDHGDGSSHQVLEDIALMRVLPNMKVVVPADPKDIERSLPVIINEERGP
LYYRIGREYSPPITIGQEYEFKIGKAYVIKDGSDLAIIGAGVVLWDALKAAEELEKLGISVAVINLFSIKPIDENTIEYY
ARKAGKIITIEEHSIYGGIGSAVAEVTARRYPVPIRFVGATTFGRSARSQRDLLDYYNINYKTIIREAIDLLK

Sequences:

>Translated_313_residues
MMQGNIYSMRETFGRLLADLGDKNKDLVVITADVGDSTRALYFREKFKDRYFNVGIAEQDMVNFAAGLAAVGKKPAIVNF
GMFLMRAWEQIRNSIARMNLDVKMFVTHTGYSDHGDGSSHQVLEDIALMRVLPNMKVVVPADPKDIERSLPVIINEERGP
LYYRIGREYSPPITIGQEYEFKIGKAYVIKDGSDLAIIGAGVVLWDALKAAEELEKLGISVAVINLFSIKPIDENTIEYY
ARKAGKIITIEEHSIYGGIGSAVAEVTARRYPVPIRFVGATTFGRSARSQRDLLDYYNINYKTIIREAIDLLK
>Mature_313_residues
MMQGNIYSMRETFGRLLADLGDKNKDLVVITADVGDSTRALYFREKFKDRYFNVGIAEQDMVNFAAGLAAVGKKPAIVNF
GMFLMRAWEQIRNSIARMNLDVKMFVTHTGYSDHGDGSSHQVLEDIALMRVLPNMKVVVPADPKDIERSLPVIINEERGP
LYYRIGREYSPPITIGQEYEFKIGKAYVIKDGSDLAIIGAGVVLWDALKAAEELEKLGISVAVINLFSIKPIDENTIEYY
ARKAGKIITIEEHSIYGGIGSAVAEVTARRYPVPIRFVGATTFGRSARSQRDLLDYYNINYKTIIREAIDLLK

Specific function: Catalyzes The Acyloin Condensation Reaction Between C Atoms 2 And 3 Of Pyruvate And Glyceraldehyde 3-Phosphate To Yield 1-Deoxy-D-Xylulose-5-Phosphate (Dxp). [C]

COG id: COG3958

COG function: function code G; Transketolase, C-terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transketolase family [H]

Homologues:

Organism=Homo sapiens, GI225637461, Length=307, Percent_Identity=35.1791530944625, Blast_Score=161, Evalue=7e-40,
Organism=Homo sapiens, GI225637459, Length=307, Percent_Identity=35.1791530944625, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI225637463, Length=307, Percent_Identity=35.1791530944625, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI133778974, Length=307, Percent_Identity=34.2019543973941, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI205277463, Length=308, Percent_Identity=32.1428571428571, Blast_Score=148, Evalue=6e-36,
Organism=Homo sapiens, GI4507521, Length=308, Percent_Identity=32.1428571428571, Blast_Score=148, Evalue=6e-36,
Organism=Homo sapiens, GI4557353, Length=243, Percent_Identity=27.9835390946502, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI34101272, Length=243, Percent_Identity=27.9835390946502, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI291084858, Length=243, Percent_Identity=28.3950617283951, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786622, Length=292, Percent_Identity=32.1917808219178, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI48994911, Length=307, Percent_Identity=24.7557003257329, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788808, Length=310, Percent_Identity=25.1612903225806, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17539652, Length=308, Percent_Identity=34.4155844155844, Blast_Score=160, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17506935, Length=248, Percent_Identity=28.6290322580645, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17538422, Length=227, Percent_Identity=27.7533039647577, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319698, Length=281, Percent_Identity=27.0462633451957, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24645119, Length=319, Percent_Identity=32.2884012539185, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI45551847, Length=319, Percent_Identity=32.2884012539185, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI45550715, Length=319, Percent_Identity=32.2884012539185, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24666278, Length=314, Percent_Identity=29.9363057324841, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI160714832, Length=266, Percent_Identity=28.9473684210526, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI160714828, Length=264, Percent_Identity=28.030303030303, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21358145, Length=245, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24650940, Length=245, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR020826
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =2.2.1.1 [H]

Molecular weight: Translated: 35087; Mature: 35087

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQGNIYSMRETFGRLLADLGDKNKDLVVITADVGDSTRALYFREKFKDRYFNVGIAEQD
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHEECCCCHHH
MVNFAAGLAAVGKKPAIVNFGMFLMRAWEQIRNSIARMNLDVKMFVTHTGYSDHGDGSSH
HHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHH
QVLEDIALMRVLPNMKVVVPADPKDIERSLPVIINEERGPLYYRIGREYSPPITIGQEYE
HHHHHHHHHHHCCCCEEEECCCCHHHHCCCCEEEECCCCCEEEEECCCCCCCEEECCCEE
FKIGKAYVIKDGSDLAIIGAGVVLWDALKAAEELEKLGISVAVINLFSIKPIDENTIEYY
EEECEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHH
ARKAGKIITIEEHSIYGGIGSAVAEVTARRYPVPIRFVGATTFGRSARSQRDLLDYYNIN
HHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCC
YKTIIREAIDLLK
HHHHHHHHHHHCC
>Mature Secondary Structure
MMQGNIYSMRETFGRLLADLGDKNKDLVVITADVGDSTRALYFREKFKDRYFNVGIAEQD
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHEECCCCHHH
MVNFAAGLAAVGKKPAIVNFGMFLMRAWEQIRNSIARMNLDVKMFVTHTGYSDHGDGSSH
HHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHH
QVLEDIALMRVLPNMKVVVPADPKDIERSLPVIINEERGPLYYRIGREYSPPITIGQEYE
HHHHHHHHHHHCCCCEEEECCCCHHHHCCCCEEEECCCCCEEEEECCCCCCCEEECCCEE
FKIGKAYVIKDGSDLAIIGAGVVLWDALKAAEELEKLGISVAVINLFSIKPIDENTIEYY
EEECEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHH
ARKAGKIITIEEHSIYGGIGSAVAEVTARRYPVPIRFVGATTFGRSARSQRDLLDYYNIN
HHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCC
YKTIIREAIDLLK
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]