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The map label for this gene is tgtA

Identifier: 15897218

GI number: 15897218

Start: 234061

End: 235572

Strand: Reverse

Name: tgtA

Synonym: SSO0274

Alternate gene names: 15897218

Gene position: 235572-234061 (Counterclockwise)

Preceding gene: 15897219

Following gene: 15897213

Centisome position: 7.87

GC content: 31.88

Gene sequence:

>1512_bases
ATGACTGTATTTGAAGTAAAATATGAGGACTTAGCAGGGAGAATAGGAACCTTAAGAACAAGAAGTGGTACCTTAGAAAC
TCCAGCATTCTTTCCAGTAATTAACGTATTAAAAAAAGATGAAATATCGATAGATGAGATAAGAAATATAGGATTTAAAA
ACTTTATCACAAATTCTTACATATTATACAAAAATAACTATATAAAGGATGATATCCATAAGGAGTTACGCTCTGAAGAA
ATGATCATAATGACAGATTCAGGGGCATATCAAATTCTAGAGTATGGAGAAATAGGAATAACCAATCTCCAGATCGTGAA
TTATCAGCTTAAAATAAAACCAGATATCGGAGTAATATTAGATTTACCTACCGGGAATATAAATGATTATGATAACGCTA
AAAAGACAGTATATGAGACATTAAAAAGAGCGGAAGAAGCTTCAGAAATCATAGTAAAAAATCAAGATAACAATATCATT
TGGGTATATCCAATACAGGGAGGAAGATATCTTGATCTAGTTAAGACTTCTGCTGAAGGTCTATCTAAATTTGAACATAT
ATACAATATGGCCGCTCTTGGTAGCCCAACAGTTCTCTTAGAGAAGTACATGTATGATACTGTAATTGACATGATTTATA
CTGCTAAATCTAACATAAAAAGAGGAATCCCGTTTCATCTATTTGGAGGAGGGTTACCTCATATCATTCCATTTGCAGTA
GCGTTAGGAGTTGACAGTTTTGACTCTGCTTCATATATAATATATGCCAGAGACAATAGATATATTACTAGGACACGCGT
ATACAAATTAGAGGATTTAGAATATTTTCCATGTTCTTGTCCAATATGCTCTAAATACACACCTAAGGATTTACTTGAAA
TGAATGAGAAAGAAAGAACAAAAGCATTGGCTATTCATAACCTTTATACTATTTTAGAAGAATTTAAAGCAACTAAACAG
GCGATTAAGGAAGGAAGATTATTTGAATATCTCCAAGAAAAAGCTTACTCTCATCCAGCAGTATATTCTGCATTCAAACG
ATTGATGAAATATAAGGATTATCTAGAGAAATTTGACCCTAGAATAAGGGGAGATCCAAAAGGTTTGTTTTTATTTGACG
GTAACTCTTTACATAGGCCAGAAATTATACGTCACTCGAGATTTCTAGAAAGATACATACAAAAGAAAGATAAAATATCC
ATATATTGCTATGATAAAGCAATAAGTGATACTGCTTATGATTTCAAGGAAAAAATAAGGGAAAAAATAGCTGATCGTAA
TGAGAGCGACGTATTTATAGCAGTACCGTTTTTTGGTTTAATACCGTTAGAGATCTCAGATTCTTATCCTCTATCTCAAT
TCGAGATACCAAATGAAATAGATGAAGATGTAATAGACGATATGAAAACTAAAATCATTTCGTTCTTAAGACGTAATAAT
TACCAAAAAGTAGAGTTAATTAACTGTGAAAAACTAGGCTTACATATAGACTCTATCAGCACTTCCTCTTGA

Upstream 100 bases:

>100_bases
AAGTATCACAGAATGGAGTAGAAGGAAGCGGACCTATGGCACAAAGAATTTACGACTTATATAATGATTATATAAATAAA
AAGAAAAAGGAATTAGGTCT

Downstream 100 bases:

>100_bases
TAACTCTTTCTGCAATAATTCCATCTGCCGTTGTAAATCTTCCTCTATTCTTTTTGCCTCTTCATATAATTCATCTACGC
TAACGTTTAAGCTAAGCATT

Product: 7-cyano-7-deazaguanine tRNA-ribosyltransferase

Products: tRNAqueuine; guanine [C]

Alternate protein names: Archaeal tRNA-guanine transglycosylase

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MTVFEVKYEDLAGRIGTLRTRSGTLETPAFFPVINVLKKDEISIDEIRNIGFKNFITNSYILYKNNYIKDDIHKELRSEE
MIIMTDSGAYQILEYGEIGITNLQIVNYQLKIKPDIGVILDLPTGNINDYDNAKKTVYETLKRAEEASEIIVKNQDNNII
WVYPIQGGRYLDLVKTSAEGLSKFEHIYNMAALGSPTVLLEKYMYDTVIDMIYTAKSNIKRGIPFHLFGGGLPHIIPFAV
ALGVDSFDSASYIIYARDNRYITRTRVYKLEDLEYFPCSCPICSKYTPKDLLEMNEKERTKALAIHNLYTILEEFKATKQ
AIKEGRLFEYLQEKAYSHPAVYSAFKRLMKYKDYLEKFDPRIRGDPKGLFLFDGNSLHRPEIIRHSRFLERYIQKKDKIS
IYCYDKAISDTAYDFKEKIREKIADRNESDVFIAVPFFGLIPLEISDSYPLSQFEIPNEIDEDVIDDMKTKIISFLRRNN
YQKVELINCEKLGLHIDSISTSS

Sequences:

>Translated_503_residues
MTVFEVKYEDLAGRIGTLRTRSGTLETPAFFPVINVLKKDEISIDEIRNIGFKNFITNSYILYKNNYIKDDIHKELRSEE
MIIMTDSGAYQILEYGEIGITNLQIVNYQLKIKPDIGVILDLPTGNINDYDNAKKTVYETLKRAEEASEIIVKNQDNNII
WVYPIQGGRYLDLVKTSAEGLSKFEHIYNMAALGSPTVLLEKYMYDTVIDMIYTAKSNIKRGIPFHLFGGGLPHIIPFAV
ALGVDSFDSASYIIYARDNRYITRTRVYKLEDLEYFPCSCPICSKYTPKDLLEMNEKERTKALAIHNLYTILEEFKATKQ
AIKEGRLFEYLQEKAYSHPAVYSAFKRLMKYKDYLEKFDPRIRGDPKGLFLFDGNSLHRPEIIRHSRFLERYIQKKDKIS
IYCYDKAISDTAYDFKEKIREKIADRNESDVFIAVPFFGLIPLEISDSYPLSQFEIPNEIDEDVIDDMKTKIISFLRRNN
YQKVELINCEKLGLHIDSISTSS
>Mature_502_residues
TVFEVKYEDLAGRIGTLRTRSGTLETPAFFPVINVLKKDEISIDEIRNIGFKNFITNSYILYKNNYIKDDIHKELRSEEM
IIMTDSGAYQILEYGEIGITNLQIVNYQLKIKPDIGVILDLPTGNINDYDNAKKTVYETLKRAEEASEIIVKNQDNNIIW
VYPIQGGRYLDLVKTSAEGLSKFEHIYNMAALGSPTVLLEKYMYDTVIDMIYTAKSNIKRGIPFHLFGGGLPHIIPFAVA
LGVDSFDSASYIIYARDNRYITRTRVYKLEDLEYFPCSCPICSKYTPKDLLEMNEKERTKALAIHNLYTILEEFKATKQA
IKEGRLFEYLQEKAYSHPAVYSAFKRLMKYKDYLEKFDPRIRGDPKGLFLFDGNSLHRPEIIRHSRFLERYIQKKDKISI
YCYDKAISDTAYDFKEKIREKIADRNESDVFIAVPFFGLIPLEISDSYPLSQFEIPNEIDEDVIDDMKTKIISFLRRNNY
QKVELINCEKLGLHIDSISTSS

Specific function: Exchanges the guanine residue with 7-cyano-7- deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D- loop) of archaeal tRNAs

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the archaeosine tRNA-ribosyltransferase family

Homologues:

Organism=Escherichia coli, GI1786607, Length=368, Percent_Identity=24.1847826086957, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ATGT_SULSO (Q980L7)

Other databases:

- EMBL:   AE006641
- PIR:   F90169
- RefSeq:   NP_341823.1
- ProteinModelPortal:   Q980L7
- SMR:   Q980L7
- GeneID:   1455423
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0274
- NMPDR:   fig|273057.1.peg.246
- HOGENOM:   HBG512800
- OMA:   ITNSYIT
- ProtClustDB:   PRK13533
- BioCyc:   SSOL273057:SSO0274-MONOMER
- HAMAP:   MF_01634
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: 2.4.2.29 [C]

Molecular weight: Translated: 58319; Mature: 58187

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS50890 PUA

Important sites: ACT_SITE 86-86

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVFEVKYEDLAGRIGTLRTRSGTLETPAFFPVINVLKKDEISIDEIRNIGFKNFITNSY
CCEEEEEHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCHHHHHCCCE
ILYKNNYIKDDIHKELRSEEMIIMTDSGAYQILEYGEIGITNLQIVNYQLKIKPDIGVIL
EEEECCCCCHHHHHHHCCCCEEEEECCCCEEEEECCCCCCEEEEEEEEEEEECCCCEEEE
DLPTGNINDYDNAKKTVYETLKRAEEASEIIVKNQDNNIIWVYPIQGGRYLDLVKTSAEG
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCEEEHHHHHHHH
LSKFEHIYNMAALGSPTVLLEKYMYDTVIDMIYTAKSNIKRGIPFHLFGGGLPHIIPFAV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHH
ALGVDSFDSASYIIYARDNRYITRTRVYKLEDLEYFPCSCPICSKYTPKDLLEMNEKERT
HHCCCCCCCCEEEEEECCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCHHHHH
KALAIHNLYTILEEFKATKQAIKEGRLFEYLQEKAYSHPAVYSAFKRLMKYKDYLEKFDP
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
RIRGDPKGLFLFDGNSLHRPEIIRHSRFLERYIQKKDKISIYCYDKAISDTAYDFKEKIR
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHH
EKIADRNESDVFIAVPFFGLIPLEISDSYPLSQFEIPNEIDEDVIDDMKTKIISFLRRNN
HHHHCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
YQKVELINCEKLGLHIDSISTSS
CCEEEEECHHHHCEEEECCCCCC
>Mature Secondary Structure 
TVFEVKYEDLAGRIGTLRTRSGTLETPAFFPVINVLKKDEISIDEIRNIGFKNFITNSY
CEEEEEHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCHHHHHCCCE
ILYKNNYIKDDIHKELRSEEMIIMTDSGAYQILEYGEIGITNLQIVNYQLKIKPDIGVIL
EEEECCCCCHHHHHHHCCCCEEEEECCCCEEEEECCCCCCEEEEEEEEEEEECCCCEEEE
DLPTGNINDYDNAKKTVYETLKRAEEASEIIVKNQDNNIIWVYPIQGGRYLDLVKTSAEG
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCEEEHHHHHHHH
LSKFEHIYNMAALGSPTVLLEKYMYDTVIDMIYTAKSNIKRGIPFHLFGGGLPHIIPFAV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHH
ALGVDSFDSASYIIYARDNRYITRTRVYKLEDLEYFPCSCPICSKYTPKDLLEMNEKERT
HHCCCCCCCCEEEEEECCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCHHHHH
KALAIHNLYTILEEFKATKQAIKEGRLFEYLQEKAYSHPAVYSAFKRLMKYKDYLEKFDP
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
RIRGDPKGLFLFDGNSLHRPEIIRHSRFLERYIQKKDKISIYCYDKAISDTAYDFKEKIR
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHH
EKIADRNESDVFIAVPFFGLIPLEISDSYPLSQFEIPNEIDEDVIDDMKTKIISFLRRNN
HHHHCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
YQKVELINCEKLGLHIDSISTSS
CCEEEEECHHHHCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn; Mg [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.00006 {guanine}} 0.000053 {guanine}} 0.000014 {7-(aminomethyl)-7-deazaguanine}} [C]

Substrates: tRNAguanine; queuine [C]

Specific reaction: tRNAguanine + queuine = tRNAqueuine + guanine [C]

General reaction: Pentosyl group transfer [C]

Inhibitor: Bisimidoester; Dimethyl suberimidate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726