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The map label for this gene is dapE [H]

Identifier: 15893270

GI number: 15893270

Start: 1249426

End: 1250574

Strand: Reverse

Name: dapE [H]

Synonym: RC1347

Alternate gene names: 15893270

Gene position: 1250574-1249426 (Counterclockwise)

Preceding gene: 15893271

Following gene: 15893269

Centisome position: 98.57

GC content: 32.72

Gene sequence:

>1149_bases
TTGATGTACATTAACTACTTAAAAGATTTGATTGGTTTTAAGTCTGTAACTCCTAAGAGCGATGGGGCTATTGAGTATAT
TAATGATTTGCTTAAACAGCACGGCTTTAAGACCGAAATAAAAATATTCGGTGATTCTAAAAGTGAGCAAGTCACTAATC
TTTATGCTGTTTTCGGTAGTAATGAGCCTAATATTTGCTTTGTTGGTCATGTAGATGTAGTGTTGGCAGGCAATCATGAG
CTTTGGCATAATGCAAGTCCATTTAAAGTTAGCCAGCAAGATGGTAAGATATATGGCAGAGGTGCGGTGGATATGAAAGG
GGCTATAGCGTGCTTTCTAGCTGCCAGTTTAGATTTTATAAAAAATAATACCGATTTTAAAGGTTCCATTAGTTTCTTAC
TTACTAGTGATGAAGAGGGAAAAGCAAAGCATGGTACTAAAGAAATGCTGCAATATATTTATGATCAAGGATATAAGATA
AATTTTGCTATTGTCGGTGAACCTACTTGTGAAAAAGAAATAGGCGATGCAATTAAGATTGGTAGAAGAGGAAGTGTTAA
CTTTAAATTAAATATAGAAGGCTTAAGCGGGCATGTAGCTTATCCTCATAAAGCAAATAACCCATTGCCTTGCTTAATAA
TAATATTGAATGAGTTAACAAATATAAAACTTGATGAAGGTACTGAATTTTTTCAACGTTCAAATCTTGAAGTAACGAAT
ATCGAGGTCAGTAATAATACTTCAAACGTAATTCCGGCAAGTACGGAAGCATCTTTCAATATACGTTTTAATAATTTACA
TAGTGCAGAAACTTTAGCAAAACAGGTAGAAGAAATTATTAAACAGCATTGTAAAGAATATAAAGTAGATTATAAGTTAG
AATATAGTAGTTCTGCTGAAAGTTTTATTCAGAACCCTAGCGATAAAATAAAAGAATTTGCTAAAGTAGTAGAGCATACG
CTTAAAATAAAGCCTGAATTCTCTACAAGCGGCGGTACGTCGGATGCAAGATTCGTCAAAAATTATTGTCCGTTAGTAGA
GTTTGGTTTATTATCCGAAACGGCACATAAAATAAACGAATACACAAAAATTAGCGATTTACAAAAATTATATGATGTGT
ATTATAACTTTTTGATGGAAATACTTTGA

Upstream 100 bases:

>100_bases
AGAGCTTGATAATAATCAAAGTAAGATTGACAACCTTAAAAACTATATAAAACAATTTGAAAACAAACTAAAAACCACAT
TAAATTCTCTTTGGCAATAA

Downstream 100 bases:

>100_bases
TGTCATTCCCGCAGAGGCAGGAATGACATAGAACCCATGCAACAAGGCCACCCTTTTACAGGAATGGCATTACCATACGC
GGATGTGATGGAACTGGTAG

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MMYINYLKDLIGFKSVTPKSDGAIEYINDLLKQHGFKTEIKIFGDSKSEQVTNLYAVFGSNEPNICFVGHVDVVLAGNHE
LWHNASPFKVSQQDGKIYGRGAVDMKGAIACFLAASLDFIKNNTDFKGSISFLLTSDEEGKAKHGTKEMLQYIYDQGYKI
NFAIVGEPTCEKEIGDAIKIGRRGSVNFKLNIEGLSGHVAYPHKANNPLPCLIIILNELTNIKLDEGTEFFQRSNLEVTN
IEVSNNTSNVIPASTEASFNIRFNNLHSAETLAKQVEEIIKQHCKEYKVDYKLEYSSSAESFIQNPSDKIKEFAKVVEHT
LKIKPEFSTSGGTSDARFVKNYCPLVEFGLLSETAHKINEYTKISDLQKLYDVYYNFLMEIL

Sequences:

>Translated_382_residues
MMYINYLKDLIGFKSVTPKSDGAIEYINDLLKQHGFKTEIKIFGDSKSEQVTNLYAVFGSNEPNICFVGHVDVVLAGNHE
LWHNASPFKVSQQDGKIYGRGAVDMKGAIACFLAASLDFIKNNTDFKGSISFLLTSDEEGKAKHGTKEMLQYIYDQGYKI
NFAIVGEPTCEKEIGDAIKIGRRGSVNFKLNIEGLSGHVAYPHKANNPLPCLIIILNELTNIKLDEGTEFFQRSNLEVTN
IEVSNNTSNVIPASTEASFNIRFNNLHSAETLAKQVEEIIKQHCKEYKVDYKLEYSSSAESFIQNPSDKIKEFAKVVEHT
LKIKPEFSTSGGTSDARFVKNYCPLVEFGLLSETAHKINEYTKISDLQKLYDVYYNFLMEIL
>Mature_382_residues
MMYINYLKDLIGFKSVTPKSDGAIEYINDLLKQHGFKTEIKIFGDSKSEQVTNLYAVFGSNEPNICFVGHVDVVLAGNHE
LWHNASPFKVSQQDGKIYGRGAVDMKGAIACFLAASLDFIKNNTDFKGSISFLLTSDEEGKAKHGTKEMLQYIYDQGYKI
NFAIVGEPTCEKEIGDAIKIGRRGSVNFKLNIEGLSGHVAYPHKANNPLPCLIIILNELTNIKLDEGTEFFQRSNLEVTN
IEVSNNTSNVIPASTEASFNIRFNNLHSAETLAKQVEEIIKQHCKEYKVDYKLEYSSSAESFIQNPSDKIKEFAKVVEHT
LKIKPEFSTSGGTSDARFVKNYCPLVEFGLLSETAHKINEYTKISDLQKLYDVYYNFLMEIL

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=380, Percent_Identity=35.2631578947368, Blast_Score=254, Evalue=6e-69,
Organism=Escherichia coli, GI1790395, Length=351, Percent_Identity=24.5014245014245, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789236, Length=279, Percent_Identity=24.3727598566308, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 42928; Mature: 42928

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMYINYLKDLIGFKSVTPKSDGAIEYINDLLKQHGFKTEIKIFGDSKSEQVTNLYAVFGS
CEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHEEEEEEECC
NEPNICFVGHVDVVLAGNHELWHNASPFKVSQQDGKIYGRGAVDMKGAIACFLAASLDFI
CCCCEEEEECEEEEEECCCHHCCCCCCCEEECCCCEEEECCCCCHHHHHHHHHHHHHHHH
KNNTDFKGSISFLLTSDEEGKAKHGTKEMLQYIYDQGYKINFAIVGEPTCEKEIGDAIKI
CCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHC
GRRGSVNFKLNIEGLSGHVAYPHKANNPLPCLIIILNELTNIKLDEGTEFFQRSNLEVTN
CCCCCEEEEEEEECCCCEEECCCCCCCCCCEEEEEEHHHCCCEECCCHHHHHHCCCEEEE
IEVSNNTSNVIPASTEASFNIRFNNLHSAETLAKQVEEIIKQHCKEYKVDYKLEYSSSAE
EEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHH
SFIQNPSDKIKEFAKVVEHTLKIKPEFSTSGGTSDARFVKNYCPLVEFGLLSETAHKINE
HHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHH
YTKISDLQKLYDVYYNFLMEIL
HHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MMYINYLKDLIGFKSVTPKSDGAIEYINDLLKQHGFKTEIKIFGDSKSEQVTNLYAVFGS
CEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHEEEEEEECC
NEPNICFVGHVDVVLAGNHELWHNASPFKVSQQDGKIYGRGAVDMKGAIACFLAASLDFI
CCCCEEEEECEEEEEECCCHHCCCCCCCEEECCCCEEEECCCCCHHHHHHHHHHHHHHHH
KNNTDFKGSISFLLTSDEEGKAKHGTKEMLQYIYDQGYKINFAIVGEPTCEKEIGDAIKI
CCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHC
GRRGSVNFKLNIEGLSGHVAYPHKANNPLPCLIIILNELTNIKLDEGTEFFQRSNLEVTN
CCCCCEEEEEEEECCCCEEECCCCCCCCCCEEEEEEHHHCCCEECCCHHHHHHCCCEEEE
IEVSNNTSNVIPASTEASFNIRFNNLHSAETLAKQVEEIIKQHCKEYKVDYKLEYSSSAE
EEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHH
SFIQNPSDKIKEFAKVVEHTLKIKPEFSTSGGTSDARFVKNYCPLVEFGLLSETAHKINE
HHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHH
YTKISDLQKLYDVYYNFLMEIL
HHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA