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The map label for this gene is pdhD [H]

Identifier: 15893162

GI number: 15893162

Start: 1146449

End: 1147828

Strand: Reverse

Name: pdhD [H]

Synonym: RC1239

Alternate gene names: 15893162

Gene position: 1147828-1146449 (Counterclockwise)

Preceding gene: 15893163

Following gene: 15893161

Centisome position: 90.47

GC content: 36.3

Gene sequence:

>1380_bases
ATGGAACAATATGATGTAGCCGTTATAGGCGGTGGTCCAGGTGGATATGTTGCAGCAATTAGAGCAGCACAATTAAAGAA
AAAAGTTGTATTAATAGAAAAAGAGCATTTGGGTGGGGTATGTCTTAATTGGGGGTGTATACCGACTAAATCCCTGCTTA
AATCTGCTGAGGTTTTTGAATATATAAAACATGCTAAAGATTATGGAATTGATGCTAAAGGTGCTGAAATCAACATTAAG
AAAATAGTCGAACGCTCAAGAGAGATTTCAAATAAGCTTGCAGGCGGTGTTAAGTTACTACTGAAAAAAAACAAAGTGAC
CGTGATAGACGGAGTAGCAAGTCTTGCAGGAAATAAGGTAATAAATATAAATGATAAACCTATAGTAAAAGCAGGAAATA
TTATTATAGCTACCGGCGCAAGATCTAGGGTTTTAAAAGGCTTTAAGCCTGACGGTAAACAAATTTGGACTTCTAAAGAA
GCTATGATACCGCAGCATGTGCCGAAATCTATGATTATTGTCGGCTCAGGTGCAATCGGTATAGAATTCGCTTCGTTTTA
TAATAGTATTGGTGTGGATGTTACCGTAATTGAGGCTCATAATAGAATATTGCCTGCTGAAGATATGGAAATTAGCGGAA
TCGCTCGTAAGAACTTTGAGAAGAAAGGTATAAAAATTATTACAAATGCTAAGTTAATTAAGCAAACAAAATCAAAAGAT
AAGATAGAAGTTGAGTTAGAGTTAGCAGATAAAACACAAAAATTACAAGCTGAAATTTTACTTATGGCAGTAGGTATTAC
AGCTAATACGGAAAATTTAGGTCTTGAGAAAACAAAAATTAAAGTAGAAAACGGCTATATAACTACTAACGGGTTAATGC
AAACTGCGGCATCAGGAATTTATGCTATAGGCGATGTAGCAGGAGTGCCTTGTTTAGCTCATAAAGCAAGCCATGAAGGT
ATTATTGCAGCGGAAAGCATAGCGGGCTTAAAACCGCATACTATTAATAAACATAATATTCCTGGTTGCACTTATTCTTC
GCCGCAAATAGCAAGCGTCGGTTTGACTGAAGAAGCAGCAACAACCTTAGGTTATGAACTTAAAATAGGCAGATTTCCTT
TTATAGCTAACGGTAAGGCTTTAGTAAGCGGTGATAGCGATGGCTTAATTAAAACTATATTTGATGCTAAGACGGGTGAG
TTGCTTGGAGCTCATATGATAGGTTCGGAAGTTACGGAATTAATACAAGGATATGTGGTTTCAAAAAATTTAGAAGGAAC
GGAGTTGGATTTAATTAACACTATCTTCCCGCATCCGACTTTATCGGAAATGATGCATGAATCAGTATTCTCTGCTTATG
ATAGGGCAATACATATATAA

Upstream 100 bases:

>100_bases
TCAGGAGATAGTGTATGATTCCTGAGATTGCTGCGTCGATGCTGTGCATCTCCTCGCAATGACGTTAATGTGCATAACAA
ACTAACAAAATAAAAATAAA

Downstream 100 bases:

>100_bases
TACTTGATGAACTTCAAAAATTGGCTACGTCGTCTTATAAGAGCTGTGGTGCTCACGTATTAAGTATATGCTCCGCTCCT
TGCCTTATAGACTCCTTGCT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MEQYDVAVIGGGPGGYVAAIRAAQLKKKVVLIEKEHLGGVCLNWGCIPTKSLLKSAEVFEYIKHAKDYGIDAKGAEINIK
KIVERSREISNKLAGGVKLLLKKNKVTVIDGVASLAGNKVININDKPIVKAGNIIIATGARSRVLKGFKPDGKQIWTSKE
AMIPQHVPKSMIIVGSGAIGIEFASFYNSIGVDVTVIEAHNRILPAEDMEISGIARKNFEKKGIKIITNAKLIKQTKSKD
KIEVELELADKTQKLQAEILLMAVGITANTENLGLEKTKIKVENGYITTNGLMQTAASGIYAIGDVAGVPCLAHKASHEG
IIAAESIAGLKPHTINKHNIPGCTYSSPQIASVGLTEEAATTLGYELKIGRFPFIANGKALVSGDSDGLIKTIFDAKTGE
LLGAHMIGSEVTELIQGYVVSKNLEGTELDLINTIFPHPTLSEMMHESVFSAYDRAIHI

Sequences:

>Translated_459_residues
MEQYDVAVIGGGPGGYVAAIRAAQLKKKVVLIEKEHLGGVCLNWGCIPTKSLLKSAEVFEYIKHAKDYGIDAKGAEINIK
KIVERSREISNKLAGGVKLLLKKNKVTVIDGVASLAGNKVININDKPIVKAGNIIIATGARSRVLKGFKPDGKQIWTSKE
AMIPQHVPKSMIIVGSGAIGIEFASFYNSIGVDVTVIEAHNRILPAEDMEISGIARKNFEKKGIKIITNAKLIKQTKSKD
KIEVELELADKTQKLQAEILLMAVGITANTENLGLEKTKIKVENGYITTNGLMQTAASGIYAIGDVAGVPCLAHKASHEG
IIAAESIAGLKPHTINKHNIPGCTYSSPQIASVGLTEEAATTLGYELKIGRFPFIANGKALVSGDSDGLIKTIFDAKTGE
LLGAHMIGSEVTELIQGYVVSKNLEGTELDLINTIFPHPTLSEMMHESVFSAYDRAIHI
>Mature_459_residues
MEQYDVAVIGGGPGGYVAAIRAAQLKKKVVLIEKEHLGGVCLNWGCIPTKSLLKSAEVFEYIKHAKDYGIDAKGAEINIK
KIVERSREISNKLAGGVKLLLKKNKVTVIDGVASLAGNKVININDKPIVKAGNIIIATGARSRVLKGFKPDGKQIWTSKE
AMIPQHVPKSMIIVGSGAIGIEFASFYNSIGVDVTVIEAHNRILPAEDMEISGIARKNFEKKGIKIITNAKLIKQTKSKD
KIEVELELADKTQKLQAEILLMAVGITANTENLGLEKTKIKVENGYITTNGLMQTAASGIYAIGDVAGVPCLAHKASHEG
IIAAESIAGLKPHTINKHNIPGCTYSSPQIASVGLTEEAATTLGYELKIGRFPFIANGKALVSGDSDGLIKTIFDAKTGE
LLGAHMIGSEVTELIQGYVVSKNLEGTELDLINTIFPHPTLSEMMHESVFSAYDRAIHI

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=461, Percent_Identity=37.7440347071584, Blast_Score=285, Evalue=5e-77,
Organism=Homo sapiens, GI50301238, Length=454, Percent_Identity=28.8546255506608, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI33519430, Length=469, Percent_Identity=26.4392324093817, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI33519428, Length=469, Percent_Identity=26.4392324093817, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI33519426, Length=469, Percent_Identity=26.4392324093817, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI148277065, Length=469, Percent_Identity=26.4392324093817, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI148277071, Length=469, Percent_Identity=26.4392324093817, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI291045266, Length=472, Percent_Identity=25.635593220339, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI22035672, Length=472, Percent_Identity=25.2118644067797, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI291045268, Length=468, Percent_Identity=25.4273504273504, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786307, Length=450, Percent_Identity=34.6666666666667, Blast_Score=252, Evalue=3e-68,
Organism=Escherichia coli, GI87082354, Length=462, Percent_Identity=28.3549783549784, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1789915, Length=434, Percent_Identity=29.4930875576037, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI87081717, Length=464, Percent_Identity=29.0948275862069, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI32565766, Length=467, Percent_Identity=38.7580299785867, Blast_Score=298, Evalue=5e-81,
Organism=Caenorhabditis elegans, GI17557007, Length=485, Percent_Identity=25.360824742268, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI71983429, Length=436, Percent_Identity=26.1467889908257, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71983419, Length=433, Percent_Identity=25.8660508083141, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71982272, Length=483, Percent_Identity=24.6376811594203, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17559934, Length=220, Percent_Identity=30.4545454545455, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=475, Percent_Identity=37.0526315789474, Blast_Score=273, Evalue=4e-74,
Organism=Saccharomyces cerevisiae, GI6325240, Length=471, Percent_Identity=28.4501061571125, Blast_Score=162, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6325166, Length=461, Percent_Identity=25.8134490238612, Blast_Score=141, Evalue=3e-34,
Organism=Drosophila melanogaster, GI21358499, Length=470, Percent_Identity=36.5957446808511, Blast_Score=276, Evalue=3e-74,
Organism=Drosophila melanogaster, GI17737741, Length=482, Percent_Identity=23.4439834024896, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24640553, Length=471, Percent_Identity=24.8407643312102, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24640549, Length=471, Percent_Identity=24.8407643312102, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24640551, Length=470, Percent_Identity=24.8936170212766, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49195; Mature: 49195

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQYDVAVIGGGPGGYVAAIRAAQLKKKVVLIEKEHLGGVCLNWGCIPTKSLLKSAEVFE
CCCEEEEEEECCCCCHHHHHHHHHHCCEEEEEEECCCCCEEEECCCCCHHHHHHHHHHHH
YIKHAKDYGIDAKGAEINIKKIVERSREISNKLAGGVKLLLKKNKVTVIDGVASLAGNKV
HHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECHHHHCCCEE
ININDKPIVKAGNIIIATGARSRVLKGFKPDGKQIWTSKEAMIPQHVPKSMIIVGSGAIG
EECCCCCEEEECCEEEECCCHHHHHCCCCCCCCEEECCCHHCCCCCCCCEEEEEECCCCH
IEFASFYNSIGVDVTVIEAHNRILPAEDMEISGIARKNFEKKGIKIITNAKLIKQTKSKD
HHHHHHHHHCCCEEEEEECCCCCCCCCCCEECCHHHCCCCCCCEEEEECCHHHHHCCCCC
KIEVELELADKTQKLQAEILLMAVGITANTENLGLEKTKIKVENGYITTNGLMQTAASGI
EEEEEEEECCCHHHHHHEEEEEEEEEEECCCCCCCEEEEEEEECCEEEECHHHHHHHCCC
YAIGDVAGVPCLAHKASHEGIIAAESIAGLKPHTINKHNIPGCTYSSPQIASVGLTEEAA
EEEHHHCCCCHHHHCCCCCCEEEEHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHH
TTLGYELKIGRFPFIANGKALVSGDSDGLIKTIFDAKTGELLGAHMIGSEVTELIQGYVV
HHCCEEEEECCCCEEECCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCEEE
SKNLEGTELDLINTIFPHPTLSEMMHESVFSAYDRAIHI
ECCCCCCEEHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEQYDVAVIGGGPGGYVAAIRAAQLKKKVVLIEKEHLGGVCLNWGCIPTKSLLKSAEVFE
CCCEEEEEEECCCCCHHHHHHHHHHCCEEEEEEECCCCCEEEECCCCCHHHHHHHHHHHH
YIKHAKDYGIDAKGAEINIKKIVERSREISNKLAGGVKLLLKKNKVTVIDGVASLAGNKV
HHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECHHHHCCCEE
ININDKPIVKAGNIIIATGARSRVLKGFKPDGKQIWTSKEAMIPQHVPKSMIIVGSGAIG
EECCCCCEEEECCEEEECCCHHHHHCCCCCCCCEEECCCHHCCCCCCCCEEEEEECCCCH
IEFASFYNSIGVDVTVIEAHNRILPAEDMEISGIARKNFEKKGIKIITNAKLIKQTKSKD
HHHHHHHHHCCCEEEEEECCCCCCCCCCCEECCHHHCCCCCCCEEEEECCHHHHHCCCCC
KIEVELELADKTQKLQAEILLMAVGITANTENLGLEKTKIKVENGYITTNGLMQTAASGI
EEEEEEEECCCHHHHHHEEEEEEEEEEECCCCCCCEEEEEEEECCEEEECHHHHHHHCCC
YAIGDVAGVPCLAHKASHEGIIAAESIAGLKPHTINKHNIPGCTYSSPQIASVGLTEEAA
EEEHHHCCCCHHHHCCCCCCEEEEHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHH
TTLGYELKIGRFPFIANGKALVSGDSDGLIKTIFDAKTGELLGAHMIGSEVTELIQGYVV
HHCCEEEEECCCCEEECCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCEEE
SKNLEGTELDLINTIFPHPTLSEMMHESVFSAYDRAIHI
ECCCCCCEEHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]