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The map label for this gene is phaA [H]

Identifier: 15893058

GI number: 15893058

Start: 1049216

End: 1049536

Strand: Reverse

Name: phaA [H]

Synonym: RC1135

Alternate gene names: 15893058

Gene position: 1049536-1049216 (Counterclockwise)

Preceding gene: 15893059

Following gene: 15893057

Centisome position: 82.72

GC content: 43.3

Gene sequence:

>321_bases
ATGACAAAACTGGTTTATATAACTTATGCGAAAAGGACTGCTTTCGGCTCGTTTATGGGTAGCCTTAGTACAACGGCTGC
ACCTATGCTTGCTGCTCATTTAATAAAGGATATACTGCAAAACAGCAAAATCGATCCAGCTTTAGTAGGTGAGGTAATCC
TTGGACAGGTAATAACCGGGGGTAGTGGGCAGAATCCGGCGAGGCAAACTCTGATTTATGCGGGAATACCGAAAGAAGTG
CCGGGCTATACGATTAATAAAGTATGCGGCTCAGGTCTTAAAAGCGTAAGTACTTGCAGCAAATTCAATTATGATGGGTG
A

Upstream 100 bases:

>100_bases
AAAATTATTAAAAAACTCTAATGCTTATAGATGTAGCAGGCGGACATATTAGTTATTTAATAAATGATAAACTAGATAAA
TTGTTTAAGGAGTACACATT

Downstream 100 bases:

>100_bases
TAATGCAATAGTTATAGCAGGTGGGCAGGAGAATATGTCGCTCTGTACGTTAGCTATATCAGAGCAGGAGCTAAATTCGG
CGATATTAAAATGGTTGATC

Product: putative acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 106; Mature: 105

Protein sequence:

>106_residues
MTKLVYITYAKRTAFGSFMGSLSTTAAPMLAAHLIKDILQNSKIDPALVGEVILGQVITGGSGQNPARQTLIYAGIPKEV
PGYTINKVCGSGLKSVSTCSKFNYDG

Sequences:

>Translated_106_residues
MTKLVYITYAKRTAFGSFMGSLSTTAAPMLAAHLIKDILQNSKIDPALVGEVILGQVITGGSGQNPARQTLIYAGIPKEV
PGYTINKVCGSGLKSVSTCSKFNYDG
>Mature_105_residues
TKLVYITYAKRTAFGSFMGSLSTTAAPMLAAHLIKDILQNSKIDPALVGEVILGQVITGGSGQNPARQTLIYAGIPKEVP
GYTINKVCGSGLKSVSTCSKFNYDG

Specific function: Involved in the production of polyhydroxyalkonic acids (PHAs), composed primarily of 3-hydroxybutyric acid (3HB) and 3- hydroxyvaleric acid (3HV) [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=92, Percent_Identity=44.5652173913043, Blast_Score=84, Evalue=2e-17,
Organism=Homo sapiens, GI4557237, Length=99, Percent_Identity=42.4242424242424, Blast_Score=77, Evalue=4e-15,
Organism=Homo sapiens, GI167614485, Length=93, Percent_Identity=39.7849462365591, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788554, Length=90, Percent_Identity=55.5555555555556, Blast_Score=98, Evalue=1e-22,
Organism=Escherichia coli, GI87082165, Length=99, Percent_Identity=44.4444444444444, Blast_Score=84, Evalue=2e-18,
Organism=Escherichia coli, GI1787663, Length=98, Percent_Identity=35.7142857142857, Blast_Score=60, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25147385, Length=95, Percent_Identity=45.2631578947368, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535917, Length=97, Percent_Identity=40.2061855670103, Blast_Score=63, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6325229, Length=98, Percent_Identity=38.7755102040816, Blast_Score=66, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24655093, Length=93, Percent_Identity=48.3870967741936, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24640423, Length=92, Percent_Identity=43.4782608695652, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 11157; Mature: 11026

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLVYITYAKRTAFGSFMGSLSTTAAPMLAAHLIKDILQNSKIDPALVGEVILGQVITG
CCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
GSGQNPARQTLIYAGIPKEVPGYTINKVCGSGLKSVSTCSKFNYDG
CCCCCHHHHEEEECCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TKLVYITYAKRTAFGSFMGSLSTTAAPMLAAHLIKDILQNSKIDPALVGEVILGQVITG
CEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
GSGQNPARQTLIYAGIPKEVPGYTINKVCGSGLKSVSTCSKFNYDG
CCCCCHHHHEEEECCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782; 10427049 [H]