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The map label for this gene is metG [H]

Identifier: 15892969

GI number: 15892969

Start: 970434

End: 971960

Strand: Reverse

Name: metG [H]

Synonym: RC1046

Alternate gene names: 15892969

Gene position: 971960-970434 (Counterclockwise)

Preceding gene: 15892970

Following gene: 15892968

Centisome position: 76.61

GC content: 35.82

Gene sequence:

>1527_bases
ATGAACAATACTTACTACATTACTACCCCTATATATTACGTTAACGATATTCCCCATATCGGTCATGCTTATACTAGCGT
TGCAAGTGACGTAATCGCTCGTTTCATGCGACTTCGAGGTTTCGATGTAATGTTTTTAACAGGTACTGACGAACATGGGC
AGAAAGTAGAAAAAGCAGCTATTAATAAAAATATTGACCCACAAAAATTTACCGATCAAACATCCGAGAGTTTTCGTCAT
CTGATGACTGCTATGCATATTTCCAATGATGATTTTATCAGAACAACGGAACACAGACATAAAAAAGCTGTAGCTGTTTT
TTGGCAGAAATTACTAGATAACGGCACTATTTATGAAGGTTTTTATGAAGGGTGGTATGCAGTGCGAGATGAGGCTTTTT
TTGATGAGTCGGAACTTACGAGGGACGGTTTAGCACCGACGGGTGCTCCAGTTGAATGGGTTAAAGAGCCTAGCTATTTC
TTTAATCTTTCAAAATGGCAGGATAAATTGCTTGAGTTTTATGAAGCAAACCCTGATTTTATCAGACCTATATCAAGGCG
TAACGAAGTAATTAGCTTTGTTAAATCGGGTTTAAAAGATTTATCGGTATCGCGTACTACTTTTAACTGGGGAATAAAAG
TGCCAAATAATGAAAAACATGTAATTTATGTTTGGCTTGATGCACTGGCTAATTATATTTCAGCACTCGGTTATCTTGAC
GAACAAAGTAATTATAATAAGTTTTGGCCGGCTGATTTACATGTAGTCGGTAAAGATATCTTGCGTTTTCATGCCGTCTA
TTGGCCTGCTTTCTTAATGGCTGCAGAAGTTCCGCTGCCGAAAACAATTATGGCTCATGGCTGGTGGACGAATGAGGGGC
AGAAAATCTCTAAATCTCTTGGTAATACCATTGATCCGATTAAGTTAATTGATGAATTCGGTGTTGATCAAGTTAGGTAT
TTTCTGATGCGTGAAGTAACTTTTGGTGCGGACGGCAATTTTGCTCGTAGTAATTTAATTACCCGTATTAATAGCGAGTT
GTCAAACAAAATAGGTAATTTATTACAGCGTACTACATCTTTTGTTTATAAGAATAATGACGCCAAAGTGCCTTTGCTTA
CGCAAGGTGTTATAGATAAAATATATGAGCTACCGATTTTAAAAACCGCAAGTAAATTTGTCGAGCAGAATATTTTATTG
ATGGACAAAACAGAGATTAATAAAATTCTTGAAAATATTATTAATTTGGCCGAAGAGGCAAATATCTATATTGATAGCGA
AGCACCCTGGAATTTGAAAAAAACCGATCCAGATAAAATGTTAGAAGTGTTATACGCTTTACTAGAAGTTTTACGCTATA
TTGCGATAATGCTTCTGCCTTTTATACCGAGTTCAGCAAATAAAATGCTTGATCAGTTAGGGGTAAATAAAGAAGAACGT
CTATTTAAGCATTTAATACGTGATTATACTTTAACAGCGGGTAGCAATATTTTAGAACCTACCATAATATTTCCAAAATT
TGAATAG

Upstream 100 bases:

>100_bases
GCTTTAAAGAATTAAGTAATAGATTTCCTGAGAGAATAAAAACAATTAAAGCTTCAGATTTAAGTCCACTTGAAGTACAT
GAGTTAATAAAAAAGCATTT

Downstream 100 bases:

>100_bases
TGTGTCATACCGTGGCGAGACCGCAGTATGACACACCTACTAAGAGTATAATATGTTAATAGATTCACATTGTCATCTTA
ATTTACGTAGTAACTTGTCA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MNNTYYITTPIYYVNDIPHIGHAYTSVASDVIARFMRLRGFDVMFLTGTDEHGQKVEKAAINKNIDPQKFTDQTSESFRH
LMTAMHISNDDFIRTTEHRHKKAVAVFWQKLLDNGTIYEGFYEGWYAVRDEAFFDESELTRDGLAPTGAPVEWVKEPSYF
FNLSKWQDKLLEFYEANPDFIRPISRRNEVISFVKSGLKDLSVSRTTFNWGIKVPNNEKHVIYVWLDALANYISALGYLD
EQSNYNKFWPADLHVVGKDILRFHAVYWPAFLMAAEVPLPKTIMAHGWWTNEGQKISKSLGNTIDPIKLIDEFGVDQVRY
FLMREVTFGADGNFARSNLITRINSELSNKIGNLLQRTTSFVYKNNDAKVPLLTQGVIDKIYELPILKTASKFVEQNILL
MDKTEINKILENIINLAEEANIYIDSEAPWNLKKTDPDKMLEVLYALLEVLRYIAIMLLPFIPSSANKMLDQLGVNKEER
LFKHLIRDYTLTAGSNILEPTIIFPKFE

Sequences:

>Translated_508_residues
MNNTYYITTPIYYVNDIPHIGHAYTSVASDVIARFMRLRGFDVMFLTGTDEHGQKVEKAAINKNIDPQKFTDQTSESFRH
LMTAMHISNDDFIRTTEHRHKKAVAVFWQKLLDNGTIYEGFYEGWYAVRDEAFFDESELTRDGLAPTGAPVEWVKEPSYF
FNLSKWQDKLLEFYEANPDFIRPISRRNEVISFVKSGLKDLSVSRTTFNWGIKVPNNEKHVIYVWLDALANYISALGYLD
EQSNYNKFWPADLHVVGKDILRFHAVYWPAFLMAAEVPLPKTIMAHGWWTNEGQKISKSLGNTIDPIKLIDEFGVDQVRY
FLMREVTFGADGNFARSNLITRINSELSNKIGNLLQRTTSFVYKNNDAKVPLLTQGVIDKIYELPILKTASKFVEQNILL
MDKTEINKILENIINLAEEANIYIDSEAPWNLKKTDPDKMLEVLYALLEVLRYIAIMLLPFIPSSANKMLDQLGVNKEER
LFKHLIRDYTLTAGSNILEPTIIFPKFE
>Mature_508_residues
MNNTYYITTPIYYVNDIPHIGHAYTSVASDVIARFMRLRGFDVMFLTGTDEHGQKVEKAAINKNIDPQKFTDQTSESFRH
LMTAMHISNDDFIRTTEHRHKKAVAVFWQKLLDNGTIYEGFYEGWYAVRDEAFFDESELTRDGLAPTGAPVEWVKEPSYF
FNLSKWQDKLLEFYEANPDFIRPISRRNEVISFVKSGLKDLSVSRTTFNWGIKVPNNEKHVIYVWLDALANYISALGYLD
EQSNYNKFWPADLHVVGKDILRFHAVYWPAFLMAAEVPLPKTIMAHGWWTNEGQKISKSLGNTIDPIKLIDEFGVDQVRY
FLMREVTFGADGNFARSNLITRINSELSNKIGNLLQRTTSFVYKNNDAKVPLLTQGVIDKIYELPILKTASKFVEQNILL
MDKTEINKILENIINLAEEANIYIDSEAPWNLKKTDPDKMLEVLYALLEVLRYIAIMLLPFIPSSANKMLDQLGVNKEER
LFKHLIRDYTLTAGSNILEPTIIFPKFE

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=509, Percent_Identity=37.9174852652259, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI14043022, Length=561, Percent_Identity=25.8467023172906, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1788432, Length=499, Percent_Identity=24.6492985971944, Blast_Score=165, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI212645096, Length=497, Percent_Identity=38.0281690140845, Blast_Score=324, Evalue=7e-89,
Organism=Caenorhabditis elegans, GI71992781, Length=389, Percent_Identity=36.2467866323907, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17540794, Length=552, Percent_Identity=25.5434782608696, Blast_Score=139, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6321610, Length=543, Percent_Identity=33.1491712707182, Blast_Score=293, Evalue=4e-80,
Organism=Saccharomyces cerevisiae, GI6321704, Length=553, Percent_Identity=24.9547920433996, Blast_Score=127, Evalue=6e-30,
Organism=Drosophila melanogaster, GI21358483, Length=516, Percent_Identity=36.6279069767442, Blast_Score=354, Evalue=9e-98,
Organism=Drosophila melanogaster, GI24655311, Length=556, Percent_Identity=26.7985611510791, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 58649; Mature: 58649

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNTYYITTPIYYVNDIPHIGHAYTSVASDVIARFMRLRGFDVMFLTGTDEHGQKVEKAA
CCCCEEEEECEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHH
INKNIDPQKFTDQTSESFRHLMTAMHISNDDFIRTTEHRHKKAVAVFWQKLLDNGTIYEG
HCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHCCCCEEHH
FYEGWYAVRDEAFFDESELTRDGLAPTGAPVEWVKEPSYFFNLSKWQDKLLEFYEANPDF
HHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHCCCHHHEEHHHHHHHHHHHHCCCCHH
IRPISRRNEVISFVKSGLKDLSVSRTTFNWGIKVPNNEKHVIYVWLDALANYISALGYLD
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECEECCCCCCEEEEEEHHHHHHHHHHHHHHC
EQSNYNKFWPADLHVVGKDILRFHAVYWPAFLMAAEVPLPKTIMAHGWWTNEGQKISKSL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHHC
GNTIDPIKLIDEFGVDQVRYFLMREVTFGADGNFARSNLITRINSELSNKIGNLLQRTTS
CCCCCHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVYKNNDAKVPLLTQGVIDKIYELPILKTASKFVEQNILLMDKTEINKILENIINLAEEA
HHEECCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHC
NIYIDSEAPWNLKKTDPDKMLEVLYALLEVLRYIAIMLLPFIPSSANKMLDQLGVNKEER
CEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
LFKHLIRDYTLTAGSNILEPTIIFPKFE
HHHHHHHHHEECCCCCCCCCCEECCCCC
>Mature Secondary Structure
MNNTYYITTPIYYVNDIPHIGHAYTSVASDVIARFMRLRGFDVMFLTGTDEHGQKVEKAA
CCCCEEEEECEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHH
INKNIDPQKFTDQTSESFRHLMTAMHISNDDFIRTTEHRHKKAVAVFWQKLLDNGTIYEG
HCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHCCCCEEHH
FYEGWYAVRDEAFFDESELTRDGLAPTGAPVEWVKEPSYFFNLSKWQDKLLEFYEANPDF
HHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHCCCHHHEEHHHHHHHHHHHHCCCCHH
IRPISRRNEVISFVKSGLKDLSVSRTTFNWGIKVPNNEKHVIYVWLDALANYISALGYLD
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECEECCCCCCEEEEEEHHHHHHHHHHHHHHC
EQSNYNKFWPADLHVVGKDILRFHAVYWPAFLMAAEVPLPKTIMAHGWWTNEGQKISKSL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHHC
GNTIDPIKLIDEFGVDQVRYFLMREVTFGADGNFARSNLITRINSELSNKIGNLLQRTTS
CCCCCHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVYKNNDAKVPLLTQGVIDKIYELPILKTASKFVEQNILLMDKTEINKILENIINLAEEA
HHEECCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHC
NIYIDSEAPWNLKKTDPDKMLEVLYALLEVLRYIAIMLLPFIPSSANKMLDQLGVNKEER
CEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
LFKHLIRDYTLTAGSNILEPTIIFPKFE
HHHHHHHHHEECCCCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA