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The map label for this gene is murE

Identifier: 15892835

GI number: 15892835

Start: 857151

End: 858590

Strand: Reverse

Name: murE

Synonym: RC0912

Alternate gene names: 15892835

Gene position: 858590-857151 (Counterclockwise)

Preceding gene: 15892836

Following gene: 15892834

Centisome position: 67.67

GC content: 31.67

Gene sequence:

>1440_bases
ATGTCCCATAACCTAAAGCAATTATTTCAACAACATAACGTAAAAGGTTTGTCGATTAATTCTAAAACCGTTAAGGATAA
AGATATCTTTTTTGCGATTAAAGGACAGAATACTGATGGAAATGATTTTATTAAGGATGCTTTAAGCAAAGGTGCAGTAT
TAGTAATTACGGACAATAAAAAAAATATTGTTATAGATAAAGTAATTTACGTAAAAGATGTGCAGGCAGCTTTATATGAA
GCCATAGAAATATTCTATCCTAAAAAACCAAAGGATCTAATAGCCGTAACCGGTACTAACGGTAAAAGTTCGGTAGTATC
CTATATAGCTCAAACATATTCGTTACTTGGGAAAAAAGCAGCATCTATAGGTACGATAGGGGTAGAAATATTTGGCTGCG
TTAATCTTATAAATGATGTACCGGAATTAACAACTTTAGACTATTTAAGCTTTAGAAAAATTGCTCATAATTTAGCTGAA
AATGGTATAGAATATTTAGTTTTTGAAGCTTCTAGTCATGGTCTTGATCAAGCAAGACTTAGGGAAATAAAAGTAAATAT
CGCATGTTTTACTAGCTTTAGTCAGGATCATCTAGATTACCACCATACAAAAGAAAATTATTTATTAGCCAAACTGAAAT
TATTTATAAATCATTTATTACCGAACGGTATCGCAATATTAAATTCTGATATAGAAGAAATAGAGTTTGTTAAAGATTAT
CTACATAATCATAATATTAAATTTATAACCGTTGGGACAAAAGGTGATTTAGAAATAACTAGACTTAACGGTTCTTTAAA
AGGACAAAATATTAATTTTACATTTAATAATAGAGAATATAATTTTAATACCCCGATAATTGGTAGCTTTCAGGCTAGTA
ATTTATTAATTGCTGTTCTTAGCATTCATTATATTGGTTTTGCTTTTGATGATGTTATAGATTCTTTAGTAGAAGTTAAA
GCAGTAAAAGGCAGAATGGAGAGAATAGATAACACTAATATCTTCGTTGATTATGCCCACACTCCGGACGCTCTTGAAAA
AGCTTTAACGGAACTGAAAAATATTAAATTACGTGATAGTAAGCTAAGCGTAGTTTTTGGCTGCGGCGGTAATCGTGATA
AGGCAAAGAGAAGTCTTATGGGGCAAATAGCCGCAAAACGTGCCGATACCATCATAATTACAGATGATAATCCACGTCAT
GAAGACCCAAAGCTTATTAGAGCTGAAATCATAAGCGGTATAGAGAAAGCAGATTATACAGAAATAGCAAATAGAGAAGA
GGCTATTAAATACGGTATAAATAATTTAAAACAAGATGATATCTTATTAGTTGCGGGCAAAGGTCATGAAAATTACCAAA
TTATAGGCGATAAGAAATTACCTTTTGATGATGCTGAGGTTGTGAGGAAGTGTGTAAAAGTTTGTCATCCCGTGGCTTGA

Upstream 100 bases:

>100_bases
GTGGCAATCTCATGAAATAATAACAAACTCTTGAGATTGCTTCGTCAATTGCTATGCAATTTCCTCGCAATGACGGTAAC
ACGTTTTTAACATAATAAAT

Downstream 100 bases:

>100_bases
CCCATAGTACCGGACAGTTTAAAAAAAACTTGATGTCATCCCCGCGGAAGCGGGGATCCAGAAAAATAACTTTAATATTA
ATACAGAAGTTACATATTTG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSHNLKQLFQQHNVKGLSINSKTVKDKDIFFAIKGQNTDGNDFIKDALSKGAVLVITDNKKNIVIDKVIYVKDVQAALYE
AIEIFYPKKPKDLIAVTGTNGKSSVVSYIAQTYSLLGKKAASIGTIGVEIFGCVNLINDVPELTTLDYLSFRKIAHNLAE
NGIEYLVFEASSHGLDQARLREIKVNIACFTSFSQDHLDYHHTKENYLLAKLKLFINHLLPNGIAILNSDIEEIEFVKDY
LHNHNIKFITVGTKGDLEITRLNGSLKGQNINFTFNNREYNFNTPIIGSFQASNLLIAVLSIHYIGFAFDDVIDSLVEVK
AVKGRMERIDNTNIFVDYAHTPDALEKALTELKNIKLRDSKLSVVFGCGGNRDKAKRSLMGQIAAKRADTIIITDDNPRH
EDPKLIRAEIISGIEKADYTEIANREEAIKYGINNLKQDDILLVAGKGHENYQIIGDKKLPFDDAEVVRKCVKVCHPVA

Sequences:

>Translated_479_residues
MSHNLKQLFQQHNVKGLSINSKTVKDKDIFFAIKGQNTDGNDFIKDALSKGAVLVITDNKKNIVIDKVIYVKDVQAALYE
AIEIFYPKKPKDLIAVTGTNGKSSVVSYIAQTYSLLGKKAASIGTIGVEIFGCVNLINDVPELTTLDYLSFRKIAHNLAE
NGIEYLVFEASSHGLDQARLREIKVNIACFTSFSQDHLDYHHTKENYLLAKLKLFINHLLPNGIAILNSDIEEIEFVKDY
LHNHNIKFITVGTKGDLEITRLNGSLKGQNINFTFNNREYNFNTPIIGSFQASNLLIAVLSIHYIGFAFDDVIDSLVEVK
AVKGRMERIDNTNIFVDYAHTPDALEKALTELKNIKLRDSKLSVVFGCGGNRDKAKRSLMGQIAAKRADTIIITDDNPRH
EDPKLIRAEIISGIEKADYTEIANREEAIKYGINNLKQDDILLVAGKGHENYQIIGDKKLPFDDAEVVRKCVKVCHPVA
>Mature_478_residues
SHNLKQLFQQHNVKGLSINSKTVKDKDIFFAIKGQNTDGNDFIKDALSKGAVLVITDNKKNIVIDKVIYVKDVQAALYEA
IEIFYPKKPKDLIAVTGTNGKSSVVSYIAQTYSLLGKKAASIGTIGVEIFGCVNLINDVPELTTLDYLSFRKIAHNLAEN
GIEYLVFEASSHGLDQARLREIKVNIACFTSFSQDHLDYHHTKENYLLAKLKLFINHLLPNGIAILNSDIEEIEFVKDYL
HNHNIKFITVGTKGDLEITRLNGSLKGQNINFTFNNREYNFNTPIIGSFQASNLLIAVLSIHYIGFAFDDVIDSLVEVKA
VKGRMERIDNTNIFVDYAHTPDALEKALTELKNIKLRDSKLSVVFGCGGNRDKAKRSLMGQIAAKRADTIIITDDNPRHE
DPKLIRAEIISGIEKADYTEIANREEAIKYGINNLKQDDILLVAGKGHENYQIIGDKKLPFDDAEVVRKCVKVCHPVA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=483, Percent_Identity=34.3685300207039, Blast_Score=233, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_RICCN (Q92H59)

Other databases:

- EMBL:   AE006914
- PIR:   H97813
- RefSeq:   NP_360549.1
- ProteinModelPortal:   Q92H59
- SMR:   Q92H59
- GeneID:   928847
- GenomeReviews:   AE006914_GR
- KEGG:   rco:RC0912
- NMPDR:   fig|272944.1.peg.912
- OMA:   WINATNI
- ProtClustDB:   PRK00139
- BioCyc:   RCON272944:RC0912-MONOMER
- BRENDA:   6.3.2.13
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 53566; Mature: 53435

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: NA

Important sites: BINDING 21-21 BINDING 171-171 BINDING 177-177 BINDING 179-179 BINDING 372-372 BINDING 446-446 BINDING 450-450

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHNLKQLFQQHNVKGLSINSKTVKDKDIFFAIKGQNTDGNDFIKDALSKGAVLVITDNK
CCCHHHHHHHHCCCCEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCEEEEEECCC
KNIVIDKVIYVKDVQAALYEAIEIFYPKKPKDLIAVTGTNGKSSVVSYIAQTYSLLGKKA
CCEEEEEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
ASIGTIGVEIFGCVNLINDVPELTTLDYLSFRKIAHNLAENGIEYLVFEASSHGLDQARL
HCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHH
REIKVNIACFTSFSQDHLDYHHTKENYLLAKLKLFINHLLPNGIAILNSDIEEIEFVKDY
HEEEEEEEEEECCCHHHCCHHCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHH
LHNHNIKFITVGTKGDLEITRLNGSLKGQNINFTFNNREYNFNTPIIGSFQASNLLIAVL
HHCCCEEEEEECCCCCEEEEEECCCCCCCEEEEEECCCEEECCCCEEECCCHHHHHHHHH
SIHYIGFAFDDVIDSLVEVKAVKGRMERIDNTNIFVDYAHTPDALEKALTELKNIKLRDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCEEECC
KLSVVFGCGGNRDKAKRSLMGQIAAKRADTIIITDDNPRHEDPKLIRAEIISGIEKADYT
EEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCHHCCHH
EIANREEAIKYGINNLKQDDILLVAGKGHENYQIIGDKKLPFDDAEVVRKCVKVCHPVA
HHHCHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SHNLKQLFQQHNVKGLSINSKTVKDKDIFFAIKGQNTDGNDFIKDALSKGAVLVITDNK
CCHHHHHHHHCCCCEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCEEEEEECCC
KNIVIDKVIYVKDVQAALYEAIEIFYPKKPKDLIAVTGTNGKSSVVSYIAQTYSLLGKKA
CCEEEEEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
ASIGTIGVEIFGCVNLINDVPELTTLDYLSFRKIAHNLAENGIEYLVFEASSHGLDQARL
HCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHH
REIKVNIACFTSFSQDHLDYHHTKENYLLAKLKLFINHLLPNGIAILNSDIEEIEFVKDY
HEEEEEEEEEECCCHHHCCHHCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHH
LHNHNIKFITVGTKGDLEITRLNGSLKGQNINFTFNNREYNFNTPIIGSFQASNLLIAVL
HHCCCEEEEEECCCCCEEEEEECCCCCCCEEEEEECCCEEECCCCEEECCCHHHHHHHHH
SIHYIGFAFDDVIDSLVEVKAVKGRMERIDNTNIFVDYAHTPDALEKALTELKNIKLRDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCEEECC
KLSVVFGCGGNRDKAKRSLMGQIAAKRADTIIITDDNPRHEDPKLIRAEIISGIEKADYT
EEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCHHCCHH
EIANREEAIKYGINNLKQDDILLVAGKGHENYQIIGDKKLPFDDAEVVRKCVKVCHPVA
HHHCHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893