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The map label for this gene is murF

Identifier: 15892834

GI number: 15892834

Start: 855594

End: 856943

Strand: Reverse

Name: murF

Synonym: RC0911

Alternate gene names: 15892834

Gene position: 856943-855594 (Counterclockwise)

Preceding gene: 15892835

Following gene: 15892833

Centisome position: 67.54

GC content: 31.26

Gene sequence:

>1350_bases
ATGATCTGGAACTCTAAAACTTTAAGTGCTGTTCTCGGTATAACAATCTCTAATTCTATAAGTTGTAATGAAGTGCAATT
TAATTCCAAAGATGTTAAAAAAGGTAATTTATTTATAGCACTTCAAGGTAATAGAGATGGTCACGATTATGTTCTAGATG
CAATAGATAAAGGAGCAGCAGCCGTAATTATTAGTAAGCGAGTAGAGATCAATGATAAAGATAAAATTATCTTAGTCGAT
GATTGTTTTGAAGCTCTACAAAAAATGGCTTTATATAAACGAGAGAATTCAAAAGCTAAATTTATAGCTATAACAGGCAG
CGTCGGTAAAACTTCTACAAAAGAAGCACTGAAAGTATTATTACAACATGATTTTCTAGTTTTTGCTAGCCGAGGTAACT
TCAATAACAAACTCGGTATGCTTATTAATCTTGCTTCAATGGCTGATGATACGGAATATGCTATTTTTGAGCTTGGCATG
AACCATAAAGGTGAAATAAGAGAGCTGGTTCAAATATTAAAGCCAAATATTGCCATGATTACTAATATTTCAGAGGCTCA
TTTAGAATTTTTTAACTCGCTTGAAGAGATAGCTGAAGCTAAATGTGAGATTTTTGCAAATTTTAGCAAAAATGATATTG
CCGTTATTAATGCCGATACTAATTGCTATAACAAAATATTATCGATATTAAAAAACCTTTCTATCACCGATATACATAGC
TTCGGTAGATCAGCTAAAACTTCAGCAGAATTAATTTTATATGAGAACCTAGGAGAGCAAGTCTACTTAAAGTATAAGAT
AAATAAGAAAGTCTTAGATGTAACGATACCGTTTATTCCTCGGCATTTTACCGAAAATTATACCGGTGTACTTTTAATTA
TCGATATATTAGGTAAAGATATAGAGATAGCTGCAAATCACTTAGCAAATATTTCTCCTACTAAAGGAAGAGGGGAAATT
ATCAATATACAAAATTGTCGTGTAATTTGTGATTACTATAACGCAAGTCCGCAGTCAATGAAAGCTGCATTAGAATATTT
AAAACAAGTACCTGCCGAGAATAAAACTGCTATAATAGGCGATATGCTAGAGCTAGGGGAAAACTCTAAACGATTACATG
AGAAACTAGTGCAGTACATATTAGATGCCGCTTGTTCTAAGGTCTATTTAGTAGGAGTAAATACTAAATATATTAATGAT
TTACTACCTGCCAAAATAGCTAAAAAATACTTTAAAAATGTTGATGAATTAATTACGCATATTACGGATTTATTTGAAGG
TAATGAGCTAATTCTAATAAAAGGGTCAAGAGGCGTAAAACTTGATAAGATTGTTGATTATTATAAATAA

Upstream 100 bases:

>100_bases
AAACATATAAAAAGCAAGCTTTTTATATGTTTACTGCCTTCCCGCCTACGCGGGAATGACATAAACACGCCATACAACAA
CACATAAATAACAAAAACAT

Downstream 100 bases:

>100_bases
ATACACTAGGATATTCTTAATATTGTGTGGTTTGGTTGCCCGTCATTGCGCGGAAATTACGTAGTAATTGACGAAGCAAT
CTCAGGATGTTTGACGAGAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl- 2,6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MIWNSKTLSAVLGITISNSISCNEVQFNSKDVKKGNLFIALQGNRDGHDYVLDAIDKGAAAVIISKRVEINDKDKIILVD
DCFEALQKMALYKRENSKAKFIAITGSVGKTSTKEALKVLLQHDFLVFASRGNFNNKLGMLINLASMADDTEYAIFELGM
NHKGEIRELVQILKPNIAMITNISEAHLEFFNSLEEIAEAKCEIFANFSKNDIAVINADTNCYNKILSILKNLSITDIHS
FGRSAKTSAELILYENLGEQVYLKYKINKKVLDVTIPFIPRHFTENYTGVLLIIDILGKDIEIAANHLANISPTKGRGEI
INIQNCRVICDYYNASPQSMKAALEYLKQVPAENKTAIIGDMLELGENSKRLHEKLVQYILDAACSKVYLVGVNTKYIND
LLPAKIAKKYFKNVDELITHITDLFEGNELILIKGSRGVKLDKIVDYYK

Sequences:

>Translated_449_residues
MIWNSKTLSAVLGITISNSISCNEVQFNSKDVKKGNLFIALQGNRDGHDYVLDAIDKGAAAVIISKRVEINDKDKIILVD
DCFEALQKMALYKRENSKAKFIAITGSVGKTSTKEALKVLLQHDFLVFASRGNFNNKLGMLINLASMADDTEYAIFELGM
NHKGEIRELVQILKPNIAMITNISEAHLEFFNSLEEIAEAKCEIFANFSKNDIAVINADTNCYNKILSILKNLSITDIHS
FGRSAKTSAELILYENLGEQVYLKYKINKKVLDVTIPFIPRHFTENYTGVLLIIDILGKDIEIAANHLANISPTKGRGEI
INIQNCRVICDYYNASPQSMKAALEYLKQVPAENKTAIIGDMLELGENSKRLHEKLVQYILDAACSKVYLVGVNTKYIND
LLPAKIAKKYFKNVDELITHITDLFEGNELILIKGSRGVKLDKIVDYYK
>Mature_449_residues
MIWNSKTLSAVLGITISNSISCNEVQFNSKDVKKGNLFIALQGNRDGHDYVLDAIDKGAAAVIISKRVEINDKDKIILVD
DCFEALQKMALYKRENSKAKFIAITGSVGKTSTKEALKVLLQHDFLVFASRGNFNNKLGMLINLASMADDTEYAIFELGM
NHKGEIRELVQILKPNIAMITNISEAHLEFFNSLEEIAEAKCEIFANFSKNDIAVINADTNCYNKILSILKNLSITDIHS
FGRSAKTSAELILYENLGEQVYLKYKINKKVLDVTIPFIPRHFTENYTGVLLIIDILGKDIEIAANHLANISPTKGRGEI
INIQNCRVICDYYNASPQSMKAALEYLKQVPAENKTAIIGDMLELGENSKRLHEKLVQYILDAACSKVYLVGVNTKYIND
LLPAKIAKKYFKNVDELITHITDLFEGNELILIKGSRGVKLDKIVDYYK

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786274, Length=430, Percent_Identity=30.2325581395349, Blast_Score=184, Evalue=7e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURF_RICCN (Q92H60)

Other databases:

- EMBL:   AE006914
- PIR:   G97813
- RefSeq:   NP_360548.1
- ProteinModelPortal:   Q92H60
- SMR:   Q92H60
- GeneID:   928846
- GenomeReviews:   AE006914_GR
- KEGG:   rco:RC0911
- NMPDR:   fig|272944.1.peg.911
- OMA:   NHKGEIR
- ProtClustDB:   CLSK871006
- BioCyc:   RCON272944:RC0911-MONOMER
- BRENDA:   6.3.2.10
- GO:   GO:0005737
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- PANTHER:   PTHR23135:SF3
- TIGRFAMs:   TIGR01143

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.10

Molecular weight: Translated: 50286; Mature: 50286

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIWNSKTLSAVLGITISNSISCNEVQFNSKDVKKGNLFIALQGNRDGHDYVLDAIDKGAA
CCCCCHHHHHEEEEEEECCCCCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCE
AVIISKRVEINDKDKIILVDDCFEALQKMALYKRENSKAKFIAITGSVGKTSTKEALKVL
EEEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHH
LQHDFLVFASRGNFNNKLGMLINLASMADDTEYAIFELGMNHKGEIRELVQILKPNIAMI
HHCCEEEEEECCCCCCCHHHEEEEHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEE
TNISEAHLEFFNSLEEIAEAKCEIFANFSKNDIAVINADTNCYNKILSILKNLSITDIHS
ECCHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHH
FGRSAKTSAELILYENLGEQVYLKYKINKKVLDVTIPFIPRHFTENYTGVLLIIDILGKD
CCCCCCCCEEEEEEECCCCEEEEEEEECCEEEEEEECCCCHHHCCCCCEEEEEEEHHCCC
IEIAANHLANISPTKGRGEIINIQNCRVICDYYNASPQSMKAALEYLKQVPAENKTAIIG
HHHHHHHHCCCCCCCCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEHHH
DMLELGENSKRLHEKLVQYILDAACSKVYLVGVNTKYINDLLPAKIAKKYFKNVDELITH
HHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHCHHHHHHH
ITDLFEGNELILIKGSRGVKLDKIVDYYK
HHHHHCCCEEEEEECCCCCCHHHHHHHCC
>Mature Secondary Structure
MIWNSKTLSAVLGITISNSISCNEVQFNSKDVKKGNLFIALQGNRDGHDYVLDAIDKGAA
CCCCCHHHHHEEEEEEECCCCCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCE
AVIISKRVEINDKDKIILVDDCFEALQKMALYKRENSKAKFIAITGSVGKTSTKEALKVL
EEEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHH
LQHDFLVFASRGNFNNKLGMLINLASMADDTEYAIFELGMNHKGEIRELVQILKPNIAMI
HHCCEEEEEECCCCCCCHHHEEEEHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEE
TNISEAHLEFFNSLEEIAEAKCEIFANFSKNDIAVINADTNCYNKILSILKNLSITDIHS
ECCHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHH
FGRSAKTSAELILYENLGEQVYLKYKINKKVLDVTIPFIPRHFTENYTGVLLIIDILGKD
CCCCCCCCEEEEEEECCCCEEEEEEEECCEEEEEEECCCCHHHCCCCCEEEEEEEHHCCC
IEIAANHLANISPTKGRGEIINIQNCRVICDYYNASPQSMKAALEYLKQVPAENKTAIIG
HHHHHHHHCCCCCCCCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEHHH
DMLELGENSKRLHEKLVQYILDAACSKVYLVGVNTKYINDLLPAKIAKKYFKNVDELITH
HHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHCHHHHHHH
ITDLFEGNELILIKGSRGVKLDKIVDYYK
HHHHHCCCEEEEEECCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893