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The map label for this gene is addA [C]

Identifier: 15892601

GI number: 15892601

Start: 656548

End: 657243

Strand: Reverse

Name: addA [C]

Synonym: RC0678

Alternate gene names: 15892601

Gene position: 657243-656548 (Counterclockwise)

Preceding gene: 15892602

Following gene: 15892598

Centisome position: 51.8

GC content: 34.2

Gene sequence:

>696_bases
ATGGATATAAAATATAATTTAGCTGCCGCTTACCGAATAATGGCGTATTTATCGCTGGATGATCATACTTATACTCATCT
ATCCGCAAGACCTAAAAATGCCGTTTTTTACTATATTTATCCTTTTGGACTAAGATTTGAAGAAGTAACTACAGAGAATC
TTTTAAAAGTTAGTTTAGACGGACAAATTTTAGAAGGAGAAGAATATCAATATAATAAAACCGGTTACTTTATTCATGGT
AGTATTTATAAAACACGCCCTGATATTTCTGCTATTTTTCATTATCATACTCCTGCAGCTATTGCCGTTTCTGCTTTAAA
ATGCGGGCTTTTACCGATCAGTCAATGGGCATTACATTTTTACGACAGAATATCATATCATAATTATAATTCTCTAGTAC
TAGATGCCGACAAACAGAGTAGTAGACTTGTTACTGACCTTAAACAGAATTATGTGATGTTACTTCGTAATCACGGTGCT
ATTACCTGCGGAAAAACTATTCATGAAGCTATGTTTTATACTTACCATTTAGAACAAGCTTGTAAAACGCAATGCCTACT
AAATTCTACAAAAGAACAAGAGCTTATAATCCCTTCCGTAGAAATATGTAAGCAGACGGTAAAAGACTTATTATCGTTTG
AAGAAGATTTAGGCAAACGTGACTGGGCAGCTTGGCTACGACTTGTGAAGATGTGA

Upstream 100 bases:

>100_bases
CATCAATTGCTATGCAATTTCCGTAGCTCAGATGGGAAAGCTGATTCACGCAACAACATCACGCGAGAATAACATAAAAC
CAATATAGATTTTAACAATT

Downstream 100 bases:

>100_bases
TCACAATACTACGACCCCAGCGGCTTTACTTGCTTGTTTTAAGGATTTATCAAGTGTTGCTATTGGTAACCCCTAGGCAT
CATAAAGTGTTAATCCATAT

Product: hypothetical protein

Products: Dihydroxyacetone phosphate; L-Lactaldehyde [C]

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MDIKYNLAAAYRIMAYLSLDDHTYTHLSARPKNAVFYYIYPFGLRFEEVTTENLLKVSLDGQILEGEEYQYNKTGYFIHG
SIYKTRPDISAIFHYHTPAAIAVSALKCGLLPISQWALHFYDRISYHNYNSLVLDADKQSSRLVTDLKQNYVMLLRNHGA
ITCGKTIHEAMFYTYHLEQACKTQCLLNSTKEQELIIPSVEICKQTVKDLLSFEEDLGKRDWAAWLRLVKM

Sequences:

>Translated_231_residues
MDIKYNLAAAYRIMAYLSLDDHTYTHLSARPKNAVFYYIYPFGLRFEEVTTENLLKVSLDGQILEGEEYQYNKTGYFIHG
SIYKTRPDISAIFHYHTPAAIAVSALKCGLLPISQWALHFYDRISYHNYNSLVLDADKQSSRLVTDLKQNYVMLLRNHGA
ITCGKTIHEAMFYTYHLEQACKTQCLLNSTKEQELIIPSVEICKQTVKDLLSFEEDLGKRDWAAWLRLVKM
>Mature_231_residues
MDIKYNLAAAYRIMAYLSLDDHTYTHLSARPKNAVFYYIYPFGLRFEEVTTENLLKVSLDGQILEGEEYQYNKTGYFIHG
SIYKTRPDISAIFHYHTPAAIAVSALKCGLLPISQWALHFYDRISYHNYNSLVLDADKQSSRLVTDLKQNYVMLLRNHGA
ITCGKTIHEAMFYTYHLEQACKTQCLLNSTKEQELIIPSVEICKQTVKDLLSFEEDLGKRDWAAWLRLVKM

Specific function: Fucose metabolism; third step. [C]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family [H]

Homologues:

Organism=Homo sapiens, GI29826321, Length=185, Percent_Identity=35.6756756756757, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI29826319, Length=185, Percent_Identity=35.6756756756757, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI29826325, Length=185, Percent_Identity=35.6756756756757, Blast_Score=131, Evalue=5e-31,
Organism=Homo sapiens, GI29826323, Length=185, Percent_Identity=35.6756756756757, Blast_Score=131, Evalue=6e-31,
Organism=Homo sapiens, GI9951927, Length=183, Percent_Identity=35.5191256830601, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI62912453, Length=183, Percent_Identity=35.5191256830601, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI9943848, Length=183, Percent_Identity=35.5191256830601, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI297307158, Length=190, Percent_Identity=32.1052631578947, Blast_Score=111, Evalue=6e-25,
Organism=Homo sapiens, GI9257192, Length=190, Percent_Identity=32.1052631578947, Blast_Score=111, Evalue=6e-25,
Organism=Homo sapiens, GI89276787, Length=192, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI297307160, Length=192, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI9257190, Length=192, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI115533218, Length=184, Percent_Identity=38.0434782608696, Blast_Score=137, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI115533216, Length=184, Percent_Identity=38.0434782608696, Blast_Score=136, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI71989560, Length=204, Percent_Identity=28.921568627451, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71989550, Length=204, Percent_Identity=28.921568627451, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71989555, Length=204, Percent_Identity=28.921568627451, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71989541, Length=204, Percent_Identity=28.921568627451, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71989544, Length=204, Percent_Identity=28.921568627451, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI281363755, Length=222, Percent_Identity=32.4324324324324, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI281363751, Length=222, Percent_Identity=32.4324324324324, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24655690, Length=222, Percent_Identity=32.4324324324324, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24655685, Length=222, Percent_Identity=32.4324324324324, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI281363763, Length=222, Percent_Identity=32.4324324324324, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281363757, Length=222, Percent_Identity=32.4324324324324, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17136738, Length=222, Percent_Identity=32.4324324324324, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281363761, Length=222, Percent_Identity=32.4324324324324, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281363759, Length=222, Percent_Identity=32.4324324324324, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281363753, Length=222, Percent_Identity=32.4324324324324, Blast_Score=108, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: 4.1.2.17 [C]

Molecular weight: Translated: 26783; Mature: 26783

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIKYNLAAAYRIMAYLSLDDHTYTHLSARPKNAVFYYIYPFGLRFEEVTTENLLKVSLD
CCEEEHHHHHEEEHEEEEECCCEEEEEECCCCCEEEEEEEECCCEEHHCCCCCEEEEEEC
GQILEGEEYQYNKTGYFIHGSIYKTRPDISAIFHYHTPAAIAVSALKCGLLPISQWALHF
CEEECCCCEEECCCEEEEEEEEEECCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHH
YDRISYHNYNSLVLDADKQSSRLVTDLKQNYVMLLRNHGAITCGKTIHEAMFYTYHLEQA
HHHHCCCCCCEEEEECCCCCHHHHHHHHHCEEEEEECCCCEEEHHHHHHHHHHHHHHHHH
CKTQCLLNSTKEQELIIPSVEICKQTVKDLLSFEEDLGKRDWAAWLRLVKM
HHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure
MDIKYNLAAAYRIMAYLSLDDHTYTHLSARPKNAVFYYIYPFGLRFEEVTTENLLKVSLD
CCEEEHHHHHEEEHEEEEECCCEEEEEECCCCCEEEEEEEECCCEEHHCCCCCEEEEEEC
GQILEGEEYQYNKTGYFIHGSIYKTRPDISAIFHYHTPAAIAVSALKCGLLPISQWALHF
CEEECCCCEEECCCEEEEEEEEEECCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHH
YDRISYHNYNSLVLDADKQSSRLVTDLKQNYVMLLRNHGAITCGKTIHEAMFYTYHLEQA
HHHHCCCCCCEEEEECCCCCHHHHHHHHHCEEEEEECCCCEEEHHHHHHHHHHHHHHHHH
CKTQCLLNSTKEQELIIPSVEICKQTVKDLLSFEEDLGKRDWAAWLRLVKM
HHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 19.3 [C]

Specific activity: 21.0

Km value (mM): 0.7 {L-fuculose} [C]

Substrates: L-Fuculose 1-phosphate [C]

Specific reaction: L-Fuculose 1-phosphate <==> Dihydroxyacetone phosphate + L-Lactaldehyde [C]

General reaction: Elimination of an aldehyde C-C bond; Cleavage [C]

Inhibitor: EDTA; Phosphoglycolohydroxamate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]