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The map label for this gene is envZ [H]

Identifier: 15892515

GI number: 15892515

Start: 578881

End: 580320

Strand: Reverse

Name: envZ [H]

Synonym: RC0592

Alternate gene names: 15892515

Gene position: 580320-578881 (Counterclockwise)

Preceding gene: 15892516

Following gene: 15892514

Centisome position: 45.74

GC content: 28.89

Gene sequence:

>1440_bases
ATGAGGGCTATGCACTTTATATCTAAATTATTACAGCATATTTCAAAGAGCTTCAGACAAGATGAATTTAAAAGCGAGCC
TACACAGCGTATAAAAATACGTGAGCACAGACGAATCTTGAAAAATTCGCTTGTATCAAGCTCTTTGAAATATGCTTTAC
CAAGAACATTATTTGTACGGTTTATGTTTATTATAATCATTCCGATTTTAATAGGACAAATCGTTGCTATATTTTTGTTT
TACGATCGTCATTGGTATAATGTTTCATATTATACTAGTACTATTATTATCAATGAAATAGAATCATTGATTAAAGAACA
TAGAAATAATTCAAGAAAGATAACCCATTTATCGGAAAATTATCTTAATTTATCTTATCAGTTTCAGCTTAATAAAAAGT
TATCTATAAAACAGCCTAAATTAGGTGAACCTTTAACTATATTCAAAAATATTTTAGCTACAAAAATCCATGAAAAAAAT
ATAGTTAAACTAAATAAGGAAAATAAAATTGAAGTATTTTTAGAATTAAAAGATGGAGTATTATATATTACTTTTCCTGC
AAAGTTACTACTTAATCCTACAGTATATATATTTGTTTTATGGATTATATCTTTAACTGTTATATTACTTTCTGTTTCTA
TTATTTTCTCCAAAAATCAAATTAAATCAATACTCACTCTTGCGGATAGCATGGATAAATTTGGTCGAGGTGTTTTAAAA
GGCAAAAATTTTAAACCTTCAGGAGCCTTAGAAATAAGAAAAGCAGGGCTTGCTTTTTTAAAAATGAAAGCTCGTATTGA
AAAACAAATTACTAAAAAAACCACTATGCTTGCTATGATTTCTCATGACCTGAGAACTCCTTTAACAAGAATGAAGCTAC
AACTTGAATTGATGGAAGAAAGCGAAGAAATCGAAGGGTTAAAACAAGATATATTAACTATGCAGCAAATGATAAATTCT
TATTTAGATTTTGCTAAAGGTGAAAGTACAGAAGAATTTGAAGACATTTTATTATTACCTTGGATGGAACGTTTTTTTAA
CAAATGGTCACATGTAAATATTGAATTTATAAAACCTACTAATTTAGATAAACTAGAAGTATGCATTAAGCCACATTCTT
TTGAAAGAGCCTTGTCTAATTTAATCGGTAACGCTATAAAACATGGAACTAAAATTAAGATATCCTCAAAAGATAATTCA
TCTACCGTAGCTATAATTATTGAGGATAACGGTCCCGGTATAGATGATACGGAAACACACCTTGTATTTAAACCGTTTTA
TAGATCGGATAAAGCAAGACAGCTTGATAATGCAAGTAACGTAGGGCTTGGGCTTGCTATTACTAAAGAAATAATAAACG
ATCATAAAGGCTCTATTTATTTAGAAGATAGCAAGGATTTAGGCGGGCTATCGGTAAGAATCGAAATACCTAAAATATGA

Upstream 100 bases:

>100_bases
GGAGGGCTTAGTATGCGTTCTATCGATGTACAAATTACAAGAATAAGAAGCAAAATAGAAGACAACCCAAAAGAGCCGAA
ATATCTAAAAACTGTGCGTA

Downstream 100 bases:

>100_bases
CTACCGATGTCATTCCCGTGTAGATGCCAAATCGTCATTACAAGGATAGGCAAAGCCGAGGGCGTGACAATCTCAGAAAT
TTTATATTGTTTCATGAGAT

Product: osmolarity sensor protein envZ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MRAMHFISKLLQHISKSFRQDEFKSEPTQRIKIREHRRILKNSLVSSSLKYALPRTLFVRFMFIIIIPILIGQIVAIFLF
YDRHWYNVSYYTSTIIINEIESLIKEHRNNSRKITHLSENYLNLSYQFQLNKKLSIKQPKLGEPLTIFKNILATKIHEKN
IVKLNKENKIEVFLELKDGVLYITFPAKLLLNPTVYIFVLWIISLTVILLSVSIIFSKNQIKSILTLADSMDKFGRGVLK
GKNFKPSGALEIRKAGLAFLKMKARIEKQITKKTTMLAMISHDLRTPLTRMKLQLELMEESEEIEGLKQDILTMQQMINS
YLDFAKGESTEEFEDILLLPWMERFFNKWSHVNIEFIKPTNLDKLEVCIKPHSFERALSNLIGNAIKHGTKIKISSKDNS
STVAIIIEDNGPGIDDTETHLVFKPFYRSDKARQLDNASNVGLGLAITKEIINDHKGSIYLEDSKDLGGLSVRIEIPKI

Sequences:

>Translated_479_residues
MRAMHFISKLLQHISKSFRQDEFKSEPTQRIKIREHRRILKNSLVSSSLKYALPRTLFVRFMFIIIIPILIGQIVAIFLF
YDRHWYNVSYYTSTIIINEIESLIKEHRNNSRKITHLSENYLNLSYQFQLNKKLSIKQPKLGEPLTIFKNILATKIHEKN
IVKLNKENKIEVFLELKDGVLYITFPAKLLLNPTVYIFVLWIISLTVILLSVSIIFSKNQIKSILTLADSMDKFGRGVLK
GKNFKPSGALEIRKAGLAFLKMKARIEKQITKKTTMLAMISHDLRTPLTRMKLQLELMEESEEIEGLKQDILTMQQMINS
YLDFAKGESTEEFEDILLLPWMERFFNKWSHVNIEFIKPTNLDKLEVCIKPHSFERALSNLIGNAIKHGTKIKISSKDNS
STVAIIIEDNGPGIDDTETHLVFKPFYRSDKARQLDNASNVGLGLAITKEIINDHKGSIYLEDSKDLGGLSVRIEIPKI
>Mature_479_residues
MRAMHFISKLLQHISKSFRQDEFKSEPTQRIKIREHRRILKNSLVSSSLKYALPRTLFVRFMFIIIIPILIGQIVAIFLF
YDRHWYNVSYYTSTIIINEIESLIKEHRNNSRKITHLSENYLNLSYQFQLNKKLSIKQPKLGEPLTIFKNILATKIHEKN
IVKLNKENKIEVFLELKDGVLYITFPAKLLLNPTVYIFVLWIISLTVILLSVSIIFSKNQIKSILTLADSMDKFGRGVLK
GKNFKPSGALEIRKAGLAFLKMKARIEKQITKKTTMLAMISHDLRTPLTRMKLQLELMEESEEIEGLKQDILTMQQMINS
YLDFAKGESTEEFEDILLLPWMERFFNKWSHVNIEFIKPTNLDKLEVCIKPHSFERALSNLIGNAIKHGTKIKISSKDNS
STVAIIIEDNGPGIDDTETHLVFKPFYRSDKARQLDNASNVGLGLAITKEIINDHKGSIYLEDSKDLGGLSVRIEIPKI

Specific function: Member of the two-component regulatory system envZ/ompR involved in the regulation of osmoregulation (genes ompF and ompC). EnvZ functions as a membrane-associated protein kinase that phosphorylates ompR in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1789808, Length=307, Percent_Identity=29.6416938110749, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1790346, Length=272, Percent_Identity=30.1470588235294, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1787894, Length=312, Percent_Identity=25.3205128205128, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI87082128, Length=297, Percent_Identity=26.2626262626263, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1788393, Length=271, Percent_Identity=24.7232472324723, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786783, Length=248, Percent_Identity=27.4193548387097, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786912, Length=228, Percent_Identity=28.0701754385965, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786600, Length=214, Percent_Identity=28.9719626168224, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790551, Length=256, Percent_Identity=23.4375, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790861, Length=294, Percent_Identity=23.8095238095238, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55323; Mature: 55323

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAMHFISKLLQHISKSFRQDEFKSEPTQRIKIREHRRILKNSLVSSSLKYALPRTLFVR
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FMFIIIIPILIGQIVAIFLFYDRHWYNVSYYTSTIIINEIESLIKEHRNNSRKITHLSEN
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
YLNLSYQFQLNKKLSIKQPKLGEPLTIFKNILATKIHEKNIVKLNKENKIEVFLELKDGV
CEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEECCE
LYITFPAKLLLNPTVYIFVLWIISLTVILLSVSIIFSKNQIKSILTLADSMDKFGRGVLK
EEEEECHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHC
GKNFKPSGALEIRKAGLAFLKMKARIEKQITKKTTMLAMISHDLRTPLTRMKLQLELMEE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
SEEIEGLKQDILTMQQMINSYLDFAKGESTEEFEDILLLPWMERFFNKWSHVNIEFIKPT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
NLDKLEVCIKPHSFERALSNLIGNAIKHGTKIKISSKDNSSTVAIIIEDNGPGIDDTETH
CCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCE
LVFKPFYRSDKARQLDNASNVGLGLAITKEIINDHKGSIYLEDSKDLGGLSVRIEIPKI
EEEECHHCCCHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCC
>Mature Secondary Structure
MRAMHFISKLLQHISKSFRQDEFKSEPTQRIKIREHRRILKNSLVSSSLKYALPRTLFVR
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FMFIIIIPILIGQIVAIFLFYDRHWYNVSYYTSTIIINEIESLIKEHRNNSRKITHLSEN
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
YLNLSYQFQLNKKLSIKQPKLGEPLTIFKNILATKIHEKNIVKLNKENKIEVFLELKDGV
CEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEECCE
LYITFPAKLLLNPTVYIFVLWIISLTVILLSVSIIFSKNQIKSILTLADSMDKFGRGVLK
EEEEECHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHC
GKNFKPSGALEIRKAGLAFLKMKARIEKQITKKTTMLAMISHDLRTPLTRMKLQLELMEE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
SEEIEGLKQDILTMQQMINSYLDFAKGESTEEFEDILLLPWMERFFNKWSHVNIEFIKPT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
NLDKLEVCIKPHSFERALSNLIGNAIKHGTKIKISSKDNSSTVAIIIEDNGPGIDDTETH
CCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCE
LVFKPFYRSDKARQLDNASNVGLGLAITKEIINDHKGSIYLEDSKDLGGLSVRIEIPKI
EEEECHHCCCHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6292200; 2997120; 9278503; 8132603; 2277041; 2824492; 1323560; 9817206; 10426948 [H]