Click here to switch to the map view.

The map label for this gene is pyrG

Identifier: 15892446

GI number: 15892446

Start: 512570

End: 514183

Strand: Reverse

Name: pyrG

Synonym: RC0523

Alternate gene names: 15892446

Gene position: 514183-512570 (Counterclockwise)

Preceding gene: 15892447

Following gene: 15892445

Centisome position: 40.53

GC content: 32.09

Gene sequence:

>1614_bases
ATGGTACATTTCATTTTTGTTACCGGCGGCGTTGTTTCATCACTCGGTAAAGGTCTAACGGCAGCATCTCTTGCTATGCT
ATTGCAAGCGAAAGGTTTTAGAGTAAGTGTACGAAAACTAGACCCTTATCTTAACATTGACCCGGGAACTATGAATCCTC
ACGAACATGGTGAGGTATATGTCACCGATGACGGAGCAGAAACCGACCTAGATCTTGGACATTATGAACGTTTTACCGGA
GTTTCCGCATGCAAATTTGATAGCATCACAACAGGTGCGATATACTCAAAATTACTTAAAGATGAACGTTTAGGAAATTA
CGCCGGCGTTACTGTTCAAGTCATCCCGCATGTTACAAATATAATAAAAGATTTTATACTTTCAAACACCAAGGGTTTTG
ATTTTATTATTTGTGAAATAGGCGGTACAGTCGGTGATATTGAAGGTCTACCGTTTTTTGAAGCTATAAGGCAAATTGGT
AACAAACTAAAAAGCGAAAATTGTTTATTTATTCATTTAACATTATTACCTTATGTTAAAACCGCCCGTGAATTAAAAAT
AAAACCTACCCAACACTCAGTTAAAGCATTACGCGCTATAGGTATTTCACCTAATATATTAGTGTGTCGTGCAGAACGTA
ACATTTCAAAAGGCGAAATAGATAAAATATCTTTATTGTGTAATATAGAATCTGAGTATGTTGTTCCGGCAATAGATCAA
AAAAATATATATTTAGTACCGATTGCATATCATAATTCAGGTCTTGATAATAAAGTATTAAAATTTTTTAATATTAATAT
CATGCCATCTAAATTAGATAAATGGCATGATATAATTAATAGGCTAAAAGATTCTAATTCAAAAGTAAGAATCGCTATAA
TAGCTAAATATCATAAATTAAAAGATGCTTATAAATCAGTAATCGAGGCACTTGATCATGCAGGTATTTATTACAAATAC
AAAATTGATTTAGTATGGATAAATGCTGAAAATCTAACTGAAGAGAATATTAATAAAAAATTATTAGATATAGATGGGAT
TTTAGTACCAGGTGGATTTGGAGAACGGGCAACTAAAGGAACAATTATAGCAATAAAATATGCCCGTACTAATAACATAC
CTTTTTTTGGAATATGTTTCGGTATGCAACTTGCGACGATCGAAATAGCTCAGAATTTAATCGGTATTAAAGATGCAGTA
ACGGAAGAATTTAAAGTAGACGGTACAAAAATTATTGAGAAAATAAATAAAAACTGTGAAGACTCAAAAATAACAATTGA
AAATGTAAAAAAGACTATGAGGCTCGGCTCTTATCCTTGTAGTTTGGTCGCAAGTACCATTGCAGCTAATGCTTATAAAA
GCCTTGAAATAAATGAGAGACATCGTCATAGATATAAATTTAATAATGAATTTCAAAATATTTTTGAAAAACATGGAATA
GTATTTAGCGGTTTTTCAAAAGATGAAGAGATTGTAGAAATAATTGAACTACCGCTACTGCGTTGGTTCGTAGGAGTACA
ATTTCATCCGGAATTTAAATCTAAACCTTTTGAAGCTCATCCGTTATTCATTCAGTTTATTAAAGCAGCGATTGAGTATA
ACAAATGTAATTAA

Upstream 100 bases:

>100_bases
CATGTTTAGTTGAGAATGTTCCTATTTCCGTAGATACGGAAGAAGACTTAAAAAAAGCAGTAAAATTTTACGAAAATATT
AGTAAACTAGGTTTATAATA

Downstream 100 bases:

>100_bases
CAACAATATATGAGACTATTTTAAAAAATCTTGACATAGTTTTTATTATAGTCTAGGGTGCATACAAAATAATAGCCGGA
CAGTTTCTAAAAAGCGTTTG

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MVHFIFVTGGVVSSLGKGLTAASLAMLLQAKGFRVSVRKLDPYLNIDPGTMNPHEHGEVYVTDDGAETDLDLGHYERFTG
VSACKFDSITTGAIYSKLLKDERLGNYAGVTVQVIPHVTNIIKDFILSNTKGFDFIICEIGGTVGDIEGLPFFEAIRQIG
NKLKSENCLFIHLTLLPYVKTARELKIKPTQHSVKALRAIGISPNILVCRAERNISKGEIDKISLLCNIESEYVVPAIDQ
KNIYLVPIAYHNSGLDNKVLKFFNINIMPSKLDKWHDIINRLKDSNSKVRIAIIAKYHKLKDAYKSVIEALDHAGIYYKY
KIDLVWINAENLTEENINKKLLDIDGILVPGGFGERATKGTIIAIKYARTNNIPFFGICFGMQLATIEIAQNLIGIKDAV
TEEFKVDGTKIIEKINKNCEDSKITIENVKKTMRLGSYPCSLVASTIAANAYKSLEINERHRHRYKFNNEFQNIFEKHGI
VFSGFSKDEEIVEIIELPLLRWFVGVQFHPEFKSKPFEAHPLFIQFIKAAIEYNKCN

Sequences:

>Translated_537_residues
MVHFIFVTGGVVSSLGKGLTAASLAMLLQAKGFRVSVRKLDPYLNIDPGTMNPHEHGEVYVTDDGAETDLDLGHYERFTG
VSACKFDSITTGAIYSKLLKDERLGNYAGVTVQVIPHVTNIIKDFILSNTKGFDFIICEIGGTVGDIEGLPFFEAIRQIG
NKLKSENCLFIHLTLLPYVKTARELKIKPTQHSVKALRAIGISPNILVCRAERNISKGEIDKISLLCNIESEYVVPAIDQ
KNIYLVPIAYHNSGLDNKVLKFFNINIMPSKLDKWHDIINRLKDSNSKVRIAIIAKYHKLKDAYKSVIEALDHAGIYYKY
KIDLVWINAENLTEENINKKLLDIDGILVPGGFGERATKGTIIAIKYARTNNIPFFGICFGMQLATIEIAQNLIGIKDAV
TEEFKVDGTKIIEKINKNCEDSKITIENVKKTMRLGSYPCSLVASTIAANAYKSLEINERHRHRYKFNNEFQNIFEKHGI
VFSGFSKDEEIVEIIELPLLRWFVGVQFHPEFKSKPFEAHPLFIQFIKAAIEYNKCN
>Mature_537_residues
MVHFIFVTGGVVSSLGKGLTAASLAMLLQAKGFRVSVRKLDPYLNIDPGTMNPHEHGEVYVTDDGAETDLDLGHYERFTG
VSACKFDSITTGAIYSKLLKDERLGNYAGVTVQVIPHVTNIIKDFILSNTKGFDFIICEIGGTVGDIEGLPFFEAIRQIG
NKLKSENCLFIHLTLLPYVKTARELKIKPTQHSVKALRAIGISPNILVCRAERNISKGEIDKISLLCNIESEYVVPAIDQ
KNIYLVPIAYHNSGLDNKVLKFFNINIMPSKLDKWHDIINRLKDSNSKVRIAIIAKYHKLKDAYKSVIEALDHAGIYYKY
KIDLVWINAENLTEENINKKLLDIDGILVPGGFGERATKGTIIAIKYARTNNIPFFGICFGMQLATIEIAQNLIGIKDAV
TEEFKVDGTKIIEKINKNCEDSKITIENVKKTMRLGSYPCSLVASTIAANAYKSLEINERHRHRYKFNNEFQNIFEKHGI
VFSGFSKDEEIVEIIELPLLRWFVGVQFHPEFKSKPFEAHPLFIQFIKAAIEYNKCN

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI28559085, Length=558, Percent_Identity=39.9641577060932, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI28559083, Length=558, Percent_Identity=39.9641577060932, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI221316689, Length=558, Percent_Identity=39.9641577060932, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI148491070, Length=553, Percent_Identity=38.5171790235081, Blast_Score=399, Evalue=1e-111,
Organism=Escherichia coli, GI1789142, Length=539, Percent_Identity=46.9387755102041, Blast_Score=501, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI25148299, Length=604, Percent_Identity=33.2781456953642, Blast_Score=339, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6322563, Length=559, Percent_Identity=39.1771019677996, Blast_Score=406, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6319432, Length=563, Percent_Identity=38.7211367673179, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24664469, Length=558, Percent_Identity=40.1433691756272, Blast_Score=407, Evalue=1e-113,
Organism=Drosophila melanogaster, GI21357815, Length=504, Percent_Identity=38.6904761904762, Blast_Score=344, Evalue=1e-94,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_RICCN (Q92I97)

Other databases:

- EMBL:   AE006914
- PIR:   C97765
- RefSeq:   NP_360160.1
- ProteinModelPortal:   Q92I97
- SMR:   Q92I97
- GeneID:   928731
- GenomeReviews:   AE006914_GR
- KEGG:   rco:RC0523
- NMPDR:   fig|272944.1.peg.523
- OMA:   TEWLRGN
- ProtClustDB:   PRK05380
- BioCyc:   RCON272944:RC0523-MONOMER
- BRENDA:   6.3.4.2
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 60452; Mature: 60452

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I ; PS00214 FABP

Important sites: ACT_SITE 379-379 ACT_SITE 509-509 ACT_SITE 511-511

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVHFIFVTGGVVSSLGKGLTAASLAMLLQAKGFRVSVRKLDPYLNIDPGTMNPHEHGEVY
CEEEEEECCHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGVSACKFDSITTGAIYSKLLKDERLGNYAGVTVQVIPHVTN
EECCCCCCCCCCCHHHHHCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHHHH
IIKDFILSNTKGFDFIICEIGGTVGDIEGLPFFEAIRQIGNKLKSENCLFIHLTLLPYVK
HHHHHHHCCCCCCEEEEEEECCCEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEHHHHHH
TARELKIKPTQHSVKALRAIGISPNILVCRAERNISKGEIDKISLLCNIESEYVVPAIDQ
HHHHEEECCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCEEEEEECC
KNIYLVPIAYHNSGLDNKVLKFFNINIMPSKLDKWHDIINRLKDSNSKVRIAIIAKYHKL
CCEEEEEEEECCCCCCCCEEEEEEEEECHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHH
KDAYKSVIEALDHAGIYYKYKIDLVWINAENLTEENINKKLLDIDGILVPGGFGERATKG
HHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCHHCCCCEEEEECCEEECCCCCCCCCCC
TIIAIKYARTNNIPFFGICFGMQLATIEIAQNLIGIKDAVTEEFKVDGTKIIEKINKNCE
EEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
DSKITIENVKKTMRLGSYPCSLVASTIAANAYKSLEINERHRHRYKFNNEFQNIFEKHGI
CCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHCCE
VFSGFSKDEEIVEIIELPLLRWFVGVQFHPEFKSKPFEAHPLFIQFIKAAIEYNKCN
EEECCCCCHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVHFIFVTGGVVSSLGKGLTAASLAMLLQAKGFRVSVRKLDPYLNIDPGTMNPHEHGEVY
CEEEEEECCHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGVSACKFDSITTGAIYSKLLKDERLGNYAGVTVQVIPHVTN
EECCCCCCCCCCCHHHHHCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHHHH
IIKDFILSNTKGFDFIICEIGGTVGDIEGLPFFEAIRQIGNKLKSENCLFIHLTLLPYVK
HHHHHHHCCCCCCEEEEEEECCCEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEHHHHHH
TARELKIKPTQHSVKALRAIGISPNILVCRAERNISKGEIDKISLLCNIESEYVVPAIDQ
HHHHEEECCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCEEEEEECC
KNIYLVPIAYHNSGLDNKVLKFFNINIMPSKLDKWHDIINRLKDSNSKVRIAIIAKYHKL
CCEEEEEEEECCCCCCCCEEEEEEEEECHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHH
KDAYKSVIEALDHAGIYYKYKIDLVWINAENLTEENINKKLLDIDGILVPGGFGERATKG
HHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCHHCCCCEEEEECCEEECCCCCCCCCCC
TIIAIKYARTNNIPFFGICFGMQLATIEIAQNLIGIKDAVTEEFKVDGTKIIEKINKNCE
EEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
DSKITIENVKKTMRLGSYPCSLVASTIAANAYKSLEINERHRHRYKFNNEFQNIFEKHGI
CCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHCCE
VFSGFSKDEEIVEIIELPLLRWFVGVQFHPEFKSKPFEAHPLFIQFIKAAIEYNKCN
EEECCCCCHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893