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The map label for this gene is pleD [H]

Identifier: 15892244

GI number: 15892244

Start: 322657

End: 324009

Strand: Reverse

Name: pleD [H]

Synonym: RC0321

Alternate gene names: 15892244

Gene position: 324009-322657 (Counterclockwise)

Preceding gene: 15892251

Following gene: 15892243

Centisome position: 25.54

GC content: 31.34

Gene sequence:

>1353_bases
ATGACCACAATATTAGTAGTAGATGATATAGAACCCAATATTAAGTTACTAACAGCCAAACTTTTAAAAGAGTATTATAC
GGTTCTTACTGCAAATAGCGGTAAAGAAGCACTAGCAATTCTTAAAAAAGAAAAAATTGATATTATACTACTTGATGTTA
TGATGCCGGAAATGGATGGATGTGAGGTATGTAAAACGATAAAAACCGATCCGGAAACTACTCATATACCGGTTGTAATG
GTAACTGCACTTTCCGATATTGATGATCGCGTTAAAGGTCTTGAGGCAGGAGCTGATGAGTTTTTAACAAAGCCGATTAA
CGATACTGCTCTATTTGTAAGACTTAAATCGCTATCTAGAATGAAAAGCTTAATTGATGAGTTAAAGCTTCGTAATAGCA
CTAATGCATTATTAGGCGTAATAAATATTGAAATGCATGACACTTTTGCAGATAAAAAAATATTACTAATTAATGATGAT
GTGGTACAAGCTAAAAATATAAAACAAATGTTACTTAAGCTTACCCAAAACGTAAAGGTAATAAGTAATTCCGATGAATT
AGATATTATAAATGAATATACCCCCGATTTAGTAATTATCAGTAGTACGCTCGAAAATGACGATCCTTTGAGGATCAGTG
TTATTTTAAGGGGTAAAGCAGAAATAAGCGGCGTAGTAATAATTTTACAAATTGATGAAGACGGTATGCCTTTGGTTGTG
AAAGGTATTGAACTCGGTATTAATGATTATTTCGTTTATCCTATAGAGGAAAGCGAATTGCTTGCTAGAATTAGAACACA
ATTAAGACGTAAGCAATATCAAGATAATTTACGTAATGATCTTGAACAAAGTGTCAATTTAGCAGTTAAAGACGGTCTAA
CCGGCTTATTTAATCGTCGCTATTTCGATATACATCTCAAACAAATTATTGAGAAAACTAATAAAGAAAGTATTAAATTA
TATTTACTTATGTGCGATATCGACAATTTTAAACATGTAAACGATACTTACGGTCATCAAGCAGGCGATAAGGTTTTAAC
AATTGTATCCCGTATTTTGAAGAATACTCTCAGAGTAACAGACTTAATAGCAAGATTCGGCGGTGAAGAATTTACTATAC
TCTTAACGGATATAGATATTTCTAAAGCAATTGCAACTGCGGAGAGAATTAGAGTTAAAATAGAATATATGAATTTTCAC
ATTGAAGATCAAATTGAACCTTTAAAAACAACTATTTCAATCGGAGTTACGAAATATAAAAAAGAAGAATCAATAGAATC
TTTCATTGAACGTGCTGACAAAGCTATGTATGAAGCTAAAACAACTGGTAAAAATAAAGTAGTAAAATTATAA

Upstream 100 bases:

>100_bases
TAATTGAATTTGCTGAAATTTTCATATATCATACATTTAAGACTTAAAATTAAAGTATAAATATACAAATTATTTCCTTT
AAAAACAAGAATTTAATCTT

Downstream 100 bases:

>100_bases
TTGATTTTTTAAAGTAAAAAAATAATGAGCAATTCTTCCAAAAAACATCTTGATTTACCATATAGACCAGGGGTCGGCAT
GATGATATTAAATGCCAATA

Product: response regulator PleD

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MTTILVVDDIEPNIKLLTAKLLKEYYTVLTANSGKEALAILKKEKIDIILLDVMMPEMDGCEVCKTIKTDPETTHIPVVM
VTALSDIDDRVKGLEAGADEFLTKPINDTALFVRLKSLSRMKSLIDELKLRNSTNALLGVINIEMHDTFADKKILLINDD
VVQAKNIKQMLLKLTQNVKVISNSDELDIINEYTPDLVIISSTLENDDPLRISVILRGKAEISGVVIILQIDEDGMPLVV
KGIELGINDYFVYPIEESELLARIRTQLRRKQYQDNLRNDLEQSVNLAVKDGLTGLFNRRYFDIHLKQIIEKTNKESIKL
YLLMCDIDNFKHVNDTYGHQAGDKVLTIVSRILKNTLRVTDLIARFGGEEFTILLTDIDISKAIATAERIRVKIEYMNFH
IEDQIEPLKTTISIGVTKYKKEESIESFIERADKAMYEAKTTGKNKVVKL

Sequences:

>Translated_450_residues
MTTILVVDDIEPNIKLLTAKLLKEYYTVLTANSGKEALAILKKEKIDIILLDVMMPEMDGCEVCKTIKTDPETTHIPVVM
VTALSDIDDRVKGLEAGADEFLTKPINDTALFVRLKSLSRMKSLIDELKLRNSTNALLGVINIEMHDTFADKKILLINDD
VVQAKNIKQMLLKLTQNVKVISNSDELDIINEYTPDLVIISSTLENDDPLRISVILRGKAEISGVVIILQIDEDGMPLVV
KGIELGINDYFVYPIEESELLARIRTQLRRKQYQDNLRNDLEQSVNLAVKDGLTGLFNRRYFDIHLKQIIEKTNKESIKL
YLLMCDIDNFKHVNDTYGHQAGDKVLTIVSRILKNTLRVTDLIARFGGEEFTILLTDIDISKAIATAERIRVKIEYMNFH
IEDQIEPLKTTISIGVTKYKKEESIESFIERADKAMYEAKTTGKNKVVKL
>Mature_449_residues
TTILVVDDIEPNIKLLTAKLLKEYYTVLTANSGKEALAILKKEKIDIILLDVMMPEMDGCEVCKTIKTDPETTHIPVVMV
TALSDIDDRVKGLEAGADEFLTKPINDTALFVRLKSLSRMKSLIDELKLRNSTNALLGVINIEMHDTFADKKILLINDDV
VQAKNIKQMLLKLTQNVKVISNSDELDIINEYTPDLVIISSTLENDDPLRISVILRGKAEISGVVIILQIDEDGMPLVVK
GIELGINDYFVYPIEESELLARIRTQLRRKQYQDNLRNDLEQSVNLAVKDGLTGLFNRRYFDIHLKQIIEKTNKESIKLY
LLMCDIDNFKHVNDTYGHQAGDKVLTIVSRILKNTLRVTDLIARFGGEEFTILLTDIDISKAIATAERIRVKIEYMNFHI
EDQIEPLKTTISIGVTKYKKEESIESFIERADKAMYEAKTTGKNKVVKL

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=44.5121951219512, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI87081881, Length=169, Percent_Identity=39.6449704142012, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI145693134, Length=157, Percent_Identity=36.9426751592357, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786584, Length=161, Percent_Identity=32.2981366459627, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788085, Length=174, Percent_Identity=35.0574712643678, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=37.9746835443038, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788381, Length=170, Percent_Identity=31.7647058823529, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082007, Length=162, Percent_Identity=31.4814814814815, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787802, Length=208, Percent_Identity=29.8076923076923, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87082012, Length=142, Percent_Identity=28.8732394366197, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87081974, Length=149, Percent_Identity=30.2013422818792, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1786599, Length=117, Percent_Identity=37.6068376068376, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1788956, Length=179, Percent_Identity=29.608938547486, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1787056, Length=168, Percent_Identity=30.952380952381, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1787541, Length=165, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1789809, Length=141, Percent_Identity=30.4964539007092, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1788394, Length=136, Percent_Identity=33.8235294117647, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789149, Length=124, Percent_Identity=35.4838709677419, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1786784, Length=122, Percent_Identity=30.327868852459, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788905, Length=110, Percent_Identity=40.9090909090909, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790437, Length=105, Percent_Identity=40.9523809523809, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI145693157, Length=103, Percent_Identity=42.7184466019417, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787229, Length=115, Percent_Identity=33.9130434782609, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788550, Length=141, Percent_Identity=31.2056737588652, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI2367329, Length=120, Percent_Identity=38.3333333333333, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI87081977, Length=151, Percent_Identity=30.4635761589404, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1786911, Length=123, Percent_Identity=30.0813008130081, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 51041; Mature: 50910

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTILVVDDIEPNIKLLTAKLLKEYYTVLTANSGKEALAILKKEKIDIILLDVMMPEMDG
CEEEEEEECCCCCHHHHHHHHHHHHHHEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCC
CEVCKTIKTDPETTHIPVVMVTALSDIDDRVKGLEAGADEFLTKPINDTALFVRLKSLSR
HHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCHHHHCCCCCCCEEHHHHHHHHH
MKSLIDELKLRNSTNALLGVINIEMHDTFADKKILLINDDVVQAKNIKQMLLKLTQNVKV
HHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEE
ISNSDELDIINEYTPDLVIISSTLENDDPLRISVILRGKAEISGVVIILQIDEDGMPLVV
EECCCCEEEHHCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEEEECCCCCCEEE
KGIELGINDYFVYPIEESELLARIRTQLRRKQYQDNLRNDLEQSVNLAVKDGLTGLFNRR
EEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHCCE
YFDIHLKQIIEKTNKESIKLYLLMCDIDNFKHVNDTYGHQAGDKVLTIVSRILKNTLRVT
EEEHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DLIARFGGEEFTILLTDIDISKAIATAERIRVKIEYMNFHIEDQIEPLKTTISIGVTKYK
HHHHHHCCCEEEEEEEECCHHHHHHHHHHEEEEEEEEEEEECCCCCHHHHEEEECHHHHH
KEESIESFIERADKAMYEAKTTGKNKVVKL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
>Mature Secondary Structure 
TTILVVDDIEPNIKLLTAKLLKEYYTVLTANSGKEALAILKKEKIDIILLDVMMPEMDG
EEEEEEECCCCCHHHHHHHHHHHHHHEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCC
CEVCKTIKTDPETTHIPVVMVTALSDIDDRVKGLEAGADEFLTKPINDTALFVRLKSLSR
HHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCHHHHCCCCCCCEEHHHHHHHHH
MKSLIDELKLRNSTNALLGVINIEMHDTFADKKILLINDDVVQAKNIKQMLLKLTQNVKV
HHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEE
ISNSDELDIINEYTPDLVIISSTLENDDPLRISVILRGKAEISGVVIILQIDEDGMPLVV
EECCCCEEEHHCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEEEECCCCCCEEE
KGIELGINDYFVYPIEESELLARIRTQLRRKQYQDNLRNDLEQSVNLAVKDGLTGLFNRR
EEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHCCE
YFDIHLKQIIEKTNKESIKLYLLMCDIDNFKHVNDTYGHQAGDKVLTIVSRILKNTLRVT
EEEHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DLIARFGGEEFTILLTDIDISKAIATAERIRVKIEYMNFHIEDQIEPLKTTISIGVTKYK
HHHHHHCCCEEEEEEEECCHHHHHHHHHHEEEEEEEEEEEECCCCCHHHHEEEECHHHHH
KEESIESFIERADKAMYEAKTTGKNKVVKL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA