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The map label for this gene is emrB [H]

Identifier: 15892123

GI number: 15892123

Start: 206273

End: 207832

Strand: Reverse

Name: emrB [H]

Synonym: RC0200

Alternate gene names: 15892123

Gene position: 207832-206273 (Counterclockwise)

Preceding gene: 15892125

Following gene: 15892122

Centisome position: 16.38

GC content: 33.46

Gene sequence:

>1560_bases
ATGTCAAATTATAATTTACAACCGCCTGCTCTTTCTAAAAAGCAATTATTTGCTTTTTTTGGTATGGTTGTCGGCATGTT
TATGTCGGTACTTGATATTCAAATTGTCGCTAGCTCATTATCCGTAATAGCTGCCGGTCTTGCCGCTTCAAGCGATGAAC
TTTCTTGGGTTCAAACATCGTACTTAATAGCTGAAGTTATCATTATTCCTATTACCGGCTTTTTAGCAAGGTTACTTACT
ACTAGAATTTCTTATTTTATTGCAGCTTTAGGGTTTACGGTTATGAGCATATTATGTTCGCTTGCAACTAATATTGAATC
CATGATTATCTTTAGAGCATTACAAGGCTTTTTTGGCGGTGCTATGATACCGACGGTCTTTAGTACCGTTTTTATACTTT
TTCCTGCATCGCAACGTCCTATCGTTACTATCTTAATTGGACTTGTCGTAACTGTTGCCCCTACTTTAGGACCTACACTC
GGTGGATATATTACGGAGATTTTATCATGGCATTTTATGTTTTTATTAAACGTCATACCCGGTATTTTCGTATGTACGGT
AGTTTTTTTATATGGGGATTTTGATAAACCAAATTATAAATTACTGAAAAATTTTGATTTTCTCGGTATATTATTAATGG
CTTTAACGCTTGGCTTATTACAGTATGTGTTAGAAGAAGGAAACAAAAAAGGTTGGCTTGAGGATAATTTAATATTATTC
TTAAGTATTACAGTAGTTTTAGGTTTTATTTTATTAATCATTAGAGAACTAACCTGTATTAATCCAATTTTAGACTTAAA
AACATTTCTTTATAAAGACTTTACTTTTGGTTGCCTTTATTCGTTCGTTATGGGTATCGGATTATACGGGACAGTATATA
TATTACCGCTATTTCTCTTTACTATTGCCGGATATGATACATTACAAATCGGGGCTACTATGATGGTGACGGGCGGAGCA
CAATTTTTATCTGCACCACTCGCCGGTAGAATGTTAGGATTAGGGGTAGATTTACGCCTGATGCTTATTATAGGTCTTGG
AGGGTTTGCTCTTGGATGTCATTTGAATAGTTTTCTAACGCCTGATTCTAAATTTGCTGCATTTGTGCTTCCTCAATTTG
TTAGAGGGCTTTCATTAATGTTTTGTTTTATACCGACTAATAATATAGCTCTTGGCAATATGCCGAAAGAAAGAGTAGGT
AACGCTAGCGGGCTTTATAATCTTACTCGCAATTTAGGAGGAGCGGTAGGGCTTGCAATAATCAGTACTATACTTACTAA
TGATACTAAAACCTTTATGCAATATTTATCGGAAAATATCTCGTCTACTTCTATAATGGCATTAGAACAACTAGATTCTT
ATACTGAATTATTAAGCGGAAAAGTTTTAAATCCCGAAAAAGCATCATATTTGTTATTAGCAAACAAATTAAATCATGAT
GCTTTTGTAATTGCAATAAATAATATATTTAATATGATAGGGCTGTTATTTGTATTTATAATGCTACTAATACCTTTTAC
TTCAAATATCAAATTAACCGAAAATGTTAATGCTCACTAA

Upstream 100 bases:

>100_bases
CAATAGTTTAATTAAAAATTAAAAGTTGTACGATGATTTTTTATTTTAAACATATATGTCACTTATTAACTTTATTTAAT
TTAAACTGTATAATTTAATT

Downstream 100 bases:

>100_bases
AATTAAATTTATATTATATTTTCCTTGGTTATTATTAGAAATATGGAAATCGGCTTTCGCGGTTATTAAAATAATTTGGC
AAAGAAAGATAGGAATAGAG

Product: multidrug resistance protein B

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MSNYNLQPPALSKKQLFAFFGMVVGMFMSVLDIQIVASSLSVIAAGLAASSDELSWVQTSYLIAEVIIIPITGFLARLLT
TRISYFIAALGFTVMSILCSLATNIESMIIFRALQGFFGGAMIPTVFSTVFILFPASQRPIVTILIGLVVTVAPTLGPTL
GGYITEILSWHFMFLLNVIPGIFVCTVVFLYGDFDKPNYKLLKNFDFLGILLMALTLGLLQYVLEEGNKKGWLEDNLILF
LSITVVLGFILLIIRELTCINPILDLKTFLYKDFTFGCLYSFVMGIGLYGTVYILPLFLFTIAGYDTLQIGATMMVTGGA
QFLSAPLAGRMLGLGVDLRLMLIIGLGGFALGCHLNSFLTPDSKFAAFVLPQFVRGLSLMFCFIPTNNIALGNMPKERVG
NASGLYNLTRNLGGAVGLAIISTILTNDTKTFMQYLSENISSTSIMALEQLDSYTELLSGKVLNPEKASYLLLANKLNHD
AFVIAINNIFNMIGLLFVFIMLLIPFTSNIKLTENVNAH

Sequences:

>Translated_519_residues
MSNYNLQPPALSKKQLFAFFGMVVGMFMSVLDIQIVASSLSVIAAGLAASSDELSWVQTSYLIAEVIIIPITGFLARLLT
TRISYFIAALGFTVMSILCSLATNIESMIIFRALQGFFGGAMIPTVFSTVFILFPASQRPIVTILIGLVVTVAPTLGPTL
GGYITEILSWHFMFLLNVIPGIFVCTVVFLYGDFDKPNYKLLKNFDFLGILLMALTLGLLQYVLEEGNKKGWLEDNLILF
LSITVVLGFILLIIRELTCINPILDLKTFLYKDFTFGCLYSFVMGIGLYGTVYILPLFLFTIAGYDTLQIGATMMVTGGA
QFLSAPLAGRMLGLGVDLRLMLIIGLGGFALGCHLNSFLTPDSKFAAFVLPQFVRGLSLMFCFIPTNNIALGNMPKERVG
NASGLYNLTRNLGGAVGLAIISTILTNDTKTFMQYLSENISSTSIMALEQLDSYTELLSGKVLNPEKASYLLLANKLNHD
AFVIAINNIFNMIGLLFVFIMLLIPFTSNIKLTENVNAH
>Mature_518_residues
SNYNLQPPALSKKQLFAFFGMVVGMFMSVLDIQIVASSLSVIAAGLAASSDELSWVQTSYLIAEVIIIPITGFLARLLTT
RISYFIAALGFTVMSILCSLATNIESMIIFRALQGFFGGAMIPTVFSTVFILFPASQRPIVTILIGLVVTVAPTLGPTLG
GYITEILSWHFMFLLNVIPGIFVCTVVFLYGDFDKPNYKLLKNFDFLGILLMALTLGLLQYVLEEGNKKGWLEDNLILFL
SITVVLGFILLIIRELTCINPILDLKTFLYKDFTFGCLYSFVMGIGLYGTVYILPLFLFTIAGYDTLQIGATMMVTGGAQ
FLSAPLAGRMLGLGVDLRLMLIIGLGGFALGCHLNSFLTPDSKFAAFVLPQFVRGLSLMFCFIPTNNIALGNMPKERVGN
ASGLYNLTRNLGGAVGLAIISTILTNDTKTFMQYLSENISSTSIMALEQLDSYTELLSGKVLNPEKASYLLLANKLNHDA
FVIAINNIFNMIGLLFVFIMLLIPFTSNIKLTENVNAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=503, Percent_Identity=28.6282306163022, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1788710, Length=502, Percent_Identity=31.2749003984064, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI1790195, Length=419, Percent_Identity=26.0143198090692, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87081983, Length=414, Percent_Identity=27.7777777777778, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1788392, Length=424, Percent_Identity=28.0660377358491, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6322958, Length=255, Percent_Identity=25.4901960784314, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6319770, Length=443, Percent_Identity=23.7020316027088, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56784; Mature: 56652

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCC
>Mature Secondary Structure 
SNYNLQPPALSKKQLFAFFGMVVGMFMSVLDIQIVASSLSVIAAGLAASSDELSWVQTS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH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EEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]