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The map label for this gene is mcl2 [H]

Identifier: 158425360

GI number: 158425360

Start: 4288079

End: 4288957

Strand: Direct

Name: mcl2 [H]

Synonym: AZC_3736

Alternate gene names: 158425360

Gene position: 4288079-4288957 (Clockwise)

Preceding gene: 158425358

Following gene: 158425361

Centisome position: 79.86

GC content: 69.62

Gene sequence:

>879_bases
ATGAGCCTTCGCCCGCGCCGCAGCGTCCTCTACATGCCCGGCTCGAACGCCCGCGCTTTGGAGAAGGCCCGCACCCTGCC
CTGCGATGGCGTGATCCTCGACCTTGAGGACGCGGTTGCGCCCGACGCCAAGGTAGAGGCCCGCTTCCGCGTGGCGGATG
CGGTGCGCGCCGGCGGCTTCGGCCCGCGCGAGACCGTCATCCGCATCAACGGCCTCGACACCCCATGGGGCACCGATGAT
CTCGCCGCCGCGGCCGCCGCGGCGCCAGATGCCATCCTTCTCCCCAAGGTGCAGGACCCGGCCCAGTTGGTGGACGTGGG
CGAGCGCCTTTCGGCCTTTGGCGTGCCCGCCACCACCAGCGTCTGGGCGATGATCGAGACGCCCATCGCCATCCTCGACA
TCAAGGCCATCTCTGCCGCCGCACTGAACCCGGTGACGCGGCTGAAGGTCTTCGTGCTCGGCACCAACGATCTCGCCAAG
GAGACCCGCGCCGCCCTCGTGCCGGGCCGCGCGCCCATGATGGGCTGGCTGATGCAGGTCATCGCCGGCGGCCATGCCTA
TGGCATCGACGTGCTGGACGGCGTGTGGAACGCCTTTCAGGATCAGGCGGGCTTTGCCGCCGAATGCTCACAGGGCGTGG
AGATGGGCATGGACGGCAAGACGCTGATCCACCCCAGCCAGATCGAGCCCTGCAACCTCGCCTTCTCGCCCCCGGCGGCG
GAAGTGGAGAAGGCTCGTGCCATCATTGCGGTGTTCGACCGGCCGGAAAATGCCGGCAAGGGCGTCGTGACGCTGGACGG
GCGCATGGTGGAGCGGATGCACGCCGACATGGCCCGCCGCACGGTCGCGCTCGCGGACGCCATTGCCGCGCGGACCTGA

Upstream 100 bases:

>100_bases
GCCTCAGGCCTTCCGGGACGGCTCCGCCTCCTTTGCAGGGCGTTGCCGGAAGCCTTCCGAATCCGCCCGACGGTTTCCTC
AAGCCTGAGAGCCCACGGAC

Downstream 100 bases:

>100_bases
ACGGGCGAGAACGTTCAGGACAACAGTTCAGCGGCCGGCGGACCCGGCCGCCCCTCACCTGAGGACAGAATGAAGCTCTA
TCGCTATCTCACCGGCCCGG

Product: citrate lyase subunit beta

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MSLRPRRSVLYMPGSNARALEKARTLPCDGVILDLEDAVAPDAKVEARFRVADAVRAGGFGPRETVIRINGLDTPWGTDD
LAAAAAAAPDAILLPKVQDPAQLVDVGERLSAFGVPATTSVWAMIETPIAILDIKAISAAALNPVTRLKVFVLGTNDLAK
ETRAALVPGRAPMMGWLMQVIAGGHAYGIDVLDGVWNAFQDQAGFAAECSQGVEMGMDGKTLIHPSQIEPCNLAFSPPAA
EVEKARAIIAVFDRPENAGKGVVTLDGRMVERMHADMARRTVALADAIAART

Sequences:

>Translated_292_residues
MSLRPRRSVLYMPGSNARALEKARTLPCDGVILDLEDAVAPDAKVEARFRVADAVRAGGFGPRETVIRINGLDTPWGTDD
LAAAAAAAPDAILLPKVQDPAQLVDVGERLSAFGVPATTSVWAMIETPIAILDIKAISAAALNPVTRLKVFVLGTNDLAK
ETRAALVPGRAPMMGWLMQVIAGGHAYGIDVLDGVWNAFQDQAGFAAECSQGVEMGMDGKTLIHPSQIEPCNLAFSPPAA
EVEKARAIIAVFDRPENAGKGVVTLDGRMVERMHADMARRTVALADAIAART
>Mature_291_residues
SLRPRRSVLYMPGSNARALEKARTLPCDGVILDLEDAVAPDAKVEARFRVADAVRAGGFGPRETVIRINGLDTPWGTDDL
AAAAAAAPDAILLPKVQDPAQLVDVGERLSAFGVPATTSVWAMIETPIAILDIKAISAAALNPVTRLKVFVLGTNDLAKE
TRAALVPGRAPMMGWLMQVIAGGHAYGIDVLDGVWNAFQDQAGFAAECSQGVEMGMDGKTLIHPSQIEPCNLAFSPPAAE
VEKARAIIAVFDRPENAGKGVVTLDGRMVERMHADMARRTVALADAIAART

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=297, Percent_Identity=28.2828282828283, Blast_Score=142, Evalue=3e-34,
Organism=Escherichia coli, GI87081764, Length=288, Percent_Identity=31.25, Blast_Score=139, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17557458, Length=285, Percent_Identity=26.6666666666667, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 30870; Mature: 30739

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRPRRSVLYMPGSNARALEKARTLPCDGVILDLEDAVAPDAKVEARFRVADAVRAGGF
CCCCCCCCEEEECCCCHHHHHHHHCCCCCCEEEECHHHCCCCCCHHHHHHHHHHHHHCCC
GPRETVIRINGLDTPWGTDDLAAAAAAAPDAILLPKVQDPAQLVDVGERLSAFGVPATTS
CCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHH
VWAMIETPIAILDIKAISAAALNPVTRLKVFVLGTNDLAKETRAALVPGRAPMMGWLMQV
HHHHHHCCEEEEEEHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHH
IAGGHAYGIDVLDGVWNAFQDQAGFAAECSQGVEMGMDGKTLIHPSQIEPCNLAFSPPAA
HHCCCEECHHHHHHHHHHHHHCCCCHHHHHCCHHCCCCCCEEECCCCCCCCCEEECCCHH
EVEKARAIIAVFDRPENAGKGVVTLDGRMVERMHADMARRTVALADAIAART
HHHHHEEEEEEEECCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLRPRRSVLYMPGSNARALEKARTLPCDGVILDLEDAVAPDAKVEARFRVADAVRAGGF
CCCCCCCEEEECCCCHHHHHHHHCCCCCCEEEECHHHCCCCCCHHHHHHHHHHHHHCCC
GPRETVIRINGLDTPWGTDDLAAAAAAAPDAILLPKVQDPAQLVDVGERLSAFGVPATTS
CCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHH
VWAMIETPIAILDIKAISAAALNPVTRLKVFVLGTNDLAKETRAALVPGRAPMMGWLMQV
HHHHHHCCEEEEEEHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHH
IAGGHAYGIDVLDGVWNAFQDQAGFAAECSQGVEMGMDGKTLIHPSQIEPCNLAFSPPAA
HHCCCEECHHHHHHHHHHHHHCCCCHHHHHCCHHCCCCCCEEECCCCCCCCCEEECCCHH
EVEKARAIIAVFDRPENAGKGVVTLDGRMVERMHADMARRTVALADAIAART
HHHHHEEEEEEEECCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0565 {oxaoacetate}} 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA