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The map label for this gene is amiC [H]

Identifier: 158425250

GI number: 158425250

Start: 4159828

End: 4161237

Strand: Reverse

Name: amiC [H]

Synonym: AZC_3626

Alternate gene names: 158425250

Gene position: 4161237-4159828 (Counterclockwise)

Preceding gene: 158425257

Following gene: 158425249

Centisome position: 77.49

GC content: 69.08

Gene sequence:

>1410_bases
GTGCCGACGCTCCAGCGTTCTCCTCTTCCCGTTCTGGCCTGCGGCGCCCTGCGTCGCGCGGGAGCGGCGATCTCGTCCTC
CGGTATCAAGATCCCGACGTTTCGTTCCGGTTCTGTGCTGGCGATATTTTTTGCAGCGGCCTTCGCCTGGTCCGCCCCGG
CCGCATGGGCGGGCGAGGCGTCGGCGGGCGCCGCTCCGGCCAAGGTCGAGGCCAAGCCCGATCCGTTGAAGGTCGCCGCC
CGTGCCGCCACATCCGGCGCCCCGACCTCGGGTCCGGCCACCGCGCAGGAGGTGCGTCTCGCGGGCGACGACCAGCGCAC
GCGCCTTGTGGTGGATCTGAGCCGGCCGGTGGAGTTCCGCGCTTTCACGCTGGCCAATCCCACCCGCGTCATCGTCGATC
TCACCGACGTGACCTTTGCGTTCAGGGCGGACAATCGCGACCTGCGGCGAGGCCTCGTTTCCGCCTATCGCTTCGGCGTC
TTCGCCCCCGGCAAGGCGCGCATGGTGCTGGACGCCTCCGATCCGGTGCTGGTGGACAAGGCCTTCGTGCTTCCCGCTCA
GGACGACCAGCCGGCCCGTCTGGTGGTGGAACTGGTGAAGACCGACGCCGACACCTTTGCCCGCTCGGTCGCGACCGCCG
CCGAACTGGCGCCGCCCCCGGCCGCCGACAGCACCCCGGCTGCGCCCGACGACCGCCGACCGGTGGTGGTGATCGACCCC
GGCCACGGCGGGCTCGATTCGGGCACGACGGGCTATTCCGCCTTCACCGAGAAGACCATCGTGCTCGATGTGGCGCTGGC
GCTGCGGGACAAGCTGGAGCGTACGGGCCGCTACCGGGTGGTGCTGACCCGTTCCACCGACGTCTTCGTGCCGCTGGGTG
AGCGCGTGCGGATCGCACGCAACAATCGCGCGGCCCTGTTCGTCTCGATCCATGCGGATGCGCTGGCGCAGGGGGATGGG
CAGGCGCGGGGCGCCAGCGTCTATACCCTGTCCGATCAGGCGAGCGATGCGGACGCGGCGCATCTGGCGGAGAAGGAGAA
CAAGGCCGACGCCATCGCGGGCATCGATTCCTCCGACGAGAGTAGCCAGGTCGCGGACATCCTCTTCGACCTCGCCCAGC
GCGAAACCAAGAGCTTTTCCATGCTGTTCGCGAAAACCCTCGTCGGGACCATGAAGACGGCGGCGCGCCTCCACAAGTCG
CCCATCAAGTCGGCGGGCTTCCGGGTGCTGAAGGCCCACGATGTCCCGTCCGTGCTGGTGGAACTCGGCTACATGTCGAG
TCCGGAAGACCTGAAGCACATGACCTCGGGGGCGTGGCGCGACAAGATGGCCGACGCCATGCAGACCGCCATCAACGACT
TCTTCGCCAGCCGTGTCGCCGGTGCCGCACCGGCCGCCGTGCCCCAGTGA

Upstream 100 bases:

>100_bases
GCGCCACAGCTTCCTGCCGGGAACGGGCGCGGGATCAACGGTGAGTTAACGCCGGCGCCCGTATCGAGTAGGACTATCGC
AGCGGATACGGAAGGTCGAA

Downstream 100 bases:

>100_bases
AAGCAGTGGATTGCCGGCACACGGTCCCGACAGCGTTACCGTCTTTTAATCCGGCTGTGGCTTCGTCCGGCCGCGATTGG
CCGTGAAGCAGCCACAGTTC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MPTLQRSPLPVLACGALRRAGAAISSSGIKIPTFRSGSVLAIFFAAAFAWSAPAAWAGEASAGAAPAKVEAKPDPLKVAA
RAATSGAPTSGPATAQEVRLAGDDQRTRLVVDLSRPVEFRAFTLANPTRVIVDLTDVTFAFRADNRDLRRGLVSAYRFGV
FAPGKARMVLDASDPVLVDKAFVLPAQDDQPARLVVELVKTDADTFARSVATAAELAPPPAADSTPAAPDDRRPVVVIDP
GHGGLDSGTTGYSAFTEKTIVLDVALALRDKLERTGRYRVVLTRSTDVFVPLGERVRIARNNRAALFVSIHADALAQGDG
QARGASVYTLSDQASDADAAHLAEKENKADAIAGIDSSDESSQVADILFDLAQRETKSFSMLFAKTLVGTMKTAARLHKS
PIKSAGFRVLKAHDVPSVLVELGYMSSPEDLKHMTSGAWRDKMADAMQTAINDFFASRVAGAAPAAVPQ

Sequences:

>Translated_469_residues
MPTLQRSPLPVLACGALRRAGAAISSSGIKIPTFRSGSVLAIFFAAAFAWSAPAAWAGEASAGAAPAKVEAKPDPLKVAA
RAATSGAPTSGPATAQEVRLAGDDQRTRLVVDLSRPVEFRAFTLANPTRVIVDLTDVTFAFRADNRDLRRGLVSAYRFGV
FAPGKARMVLDASDPVLVDKAFVLPAQDDQPARLVVELVKTDADTFARSVATAAELAPPPAADSTPAAPDDRRPVVVIDP
GHGGLDSGTTGYSAFTEKTIVLDVALALRDKLERTGRYRVVLTRSTDVFVPLGERVRIARNNRAALFVSIHADALAQGDG
QARGASVYTLSDQASDADAAHLAEKENKADAIAGIDSSDESSQVADILFDLAQRETKSFSMLFAKTLVGTMKTAARLHKS
PIKSAGFRVLKAHDVPSVLVELGYMSSPEDLKHMTSGAWRDKMADAMQTAINDFFASRVAGAAPAAVPQ
>Mature_468_residues
PTLQRSPLPVLACGALRRAGAAISSSGIKIPTFRSGSVLAIFFAAAFAWSAPAAWAGEASAGAAPAKVEAKPDPLKVAAR
AATSGAPTSGPATAQEVRLAGDDQRTRLVVDLSRPVEFRAFTLANPTRVIVDLTDVTFAFRADNRDLRRGLVSAYRFGVF
APGKARMVLDASDPVLVDKAFVLPAQDDQPARLVVELVKTDADTFARSVATAAELAPPPAADSTPAAPDDRRPVVVIDPG
HGGLDSGTTGYSAFTEKTIVLDVALALRDKLERTGRYRVVLTRSTDVFVPLGERVRIARNNRAALFVSIHADALAQGDGQ
ARGASVYTLSDQASDADAAHLAEKENKADAIAGIDSSDESSQVADILFDLAQRETKSFSMLFAKTLVGTMKTAARLHKSP
IKSAGFRVLKAHDVPSVLVELGYMSSPEDLKHMTSGAWRDKMADAMQTAINDFFASRVAGAAPAAVPQ

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=370, Percent_Identity=33.5135135135135, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI1788776, Length=243, Percent_Identity=39.0946502057613, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1790611, Length=250, Percent_Identity=28.8, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 49445; Mature: 49314

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTLQRSPLPVLACGALRRAGAAISSSGIKIPTFRSGSVLAIFFAAAFAWSAPAAWAGEA
CCCCCCCCCCEEEHHHHHHHCCCCCCCCEECCEECCCCHHHHHHHHHHHHCCCCCCCCCC
SAGAAPAKVEAKPDPLKVAARAATSGAPTSGPATAQEVRLAGDDQRTRLVVDLSRPVEFR
CCCCCCCEECCCCCCHHHHHHHHCCCCCCCCCCCHHHEEECCCCCCEEEEEEECCCCEEE
AFTLANPTRVIVDLTDVTFAFRADNRDLRRGLVSAYRFGVFAPGKARMVLDASDPVLVDK
EEEECCCCEEEEEEECEEEEEECCCHHHHHHHHHHHHHCEECCCCCEEEEECCCCEEEEE
AFVLPAQDDQPARLVVELVKTDADTFARSVATAAELAPPPAADSTPAAPDDRRPVVVIDP
EEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEC
GHGGLDSGTTGYSAFTEKTIVLDVALALRDKLERTGRYRVVLTRSTDVFVPLGERVRIAR
CCCCCCCCCCCCHHHHCCCCEEHHHHHHHHHHHHCCCEEEEEEECCEEEEECCCEEEEEE
NNRAALFVSIHADALAQGDGQARGASVYTLSDQASDADAAHLAEKENKADAIAGIDSSDE
CCCEEEEEEEECCHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCHHEECCCCCCC
SSQVADILFDLAQRETKSFSMLFAKTLVGTMKTAARLHKSPIKSAGFRVLKAHDVPSVLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEEECCHHHHHH
ELGYMSSPEDLKHMTSGAWRDKMADAMQTAINDFFASRVAGAAPAAVPQ
HHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PTLQRSPLPVLACGALRRAGAAISSSGIKIPTFRSGSVLAIFFAAAFAWSAPAAWAGEA
CCCCCCCCCEEEHHHHHHHCCCCCCCCEECCEECCCCHHHHHHHHHHHHCCCCCCCCCC
SAGAAPAKVEAKPDPLKVAARAATSGAPTSGPATAQEVRLAGDDQRTRLVVDLSRPVEFR
CCCCCCCEECCCCCCHHHHHHHHCCCCCCCCCCCHHHEEECCCCCCEEEEEEECCCCEEE
AFTLANPTRVIVDLTDVTFAFRADNRDLRRGLVSAYRFGVFAPGKARMVLDASDPVLVDK
EEEECCCCEEEEEEECEEEEEECCCHHHHHHHHHHHHHCEECCCCCEEEEECCCCEEEEE
AFVLPAQDDQPARLVVELVKTDADTFARSVATAAELAPPPAADSTPAAPDDRRPVVVIDP
EEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEC
GHGGLDSGTTGYSAFTEKTIVLDVALALRDKLERTGRYRVVLTRSTDVFVPLGERVRIAR
CCCCCCCCCCCCHHHHCCCCEEHHHHHHHHHHHHCCCEEEEEEECCEEEEECCCEEEEEE
NNRAALFVSIHADALAQGDGQARGASVYTLSDQASDADAAHLAEKENKADAIAGIDSSDE
CCCEEEEEEEECCHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCHHEECCCCCCC
SSQVADILFDLAQRETKSFSMLFAKTLVGTMKTAARLHKSPIKSAGFRVLKAHDVPSVLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEEECCHHHHHH
ELGYMSSPEDLKHMTSGAWRDKMADAMQTAINDFFASRVAGAAPAAVPQ
HHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]