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The map label for this gene is hemF [H]

Identifier: 158425127

GI number: 158425127

Start: 4019526

End: 4020425

Strand: Direct

Name: hemF [H]

Synonym: AZC_3503

Alternate gene names: 158425127

Gene position: 4019526-4020425 (Clockwise)

Preceding gene: 158425126

Following gene: 158425128

Centisome position: 74.85

GC content: 67.89

Gene sequence:

>900_bases
ATGACCGACACCGCCATCGAGACCGCCGAGCTGGAGCGCCGCAAGGCCGAGGCCCGCGCGTGGTTCGAGGCCTTGCAGGG
GCGCATCATCGCCGCCTTCGAGCAACTGGAGGACGAGGCGTCCCCAGCGCTCTACGGCGAAAAGGCCGGCCGTTTCGTGC
GCACGCCATGGGACCGCACGGACCATACGGGCGCGCCCGGCGGCGGCGGCACCATGGCCATGATGCACGGGCGGCTGTTC
GAGAAGGTGGGCGTGCATACCTCCACCGTCTTCGGTGAATTCGCGCCCGAGTTCCGGGGCCAGATTCCCGGCGCGGCGGA
AGACCCGCGCTTCTGGGCCTCCGGCATCTCGCTCATCGCCCACATGGCCAATCCCCACGTGCCGGCGGTGCATATGAACA
CCCGCTTCGTCGTCACCACCAAAAGCTGGTTCGGCGGCGGCGCCGACCTCACGCCGGTCCTCGACACCCGCCGCACGCAG
GACGATCCGGACACACGCGCCTTTCACGCCGCGTTCGAGAAGGCCTGCGGTGCCCATGCGGTGGCCGATTACGGCCGCTA
CAAGGCTTGGTGCGACGAATATTTCTTCCTCAAGCACCGCAACGAGATGCGGGGCATCGGCGGCATCTTCTACGACTGGC
TGAACTCGGGCGACTTCGGCGCCGATCTCGCCTTCACGCGGGAGGTGGGCACCACCTTCCTCGACATCTATCCCCGCCTC
GTGCGCGCCAACATGGCGCGGCCCTGGGACGAGACGGAGCGCGAGGAGCAACTCGTGCGCCGCGGCCGCTACGTGGAGTT
CAACCTGCTCTACGACCGCGGCACCATTTTCGGCCTGAAGACCGGCGGCAACATCGCCTCCATCCTCTCCTCCATGCCGC
CCGTGGTGAAGTGGCCGTAG

Upstream 100 bases:

>100_bases
AGCCCACATGATTCTCCGGCGCGGGTCCCTGCCCGCCGCTTCCGTCGGCGGACCGAACATGCTCTAGAACGCGCGGGGGC
CCGCAGGAGGTTAAGCGCCG

Downstream 100 bases:

>100_bases
GACGGCACCACCGCCGTTGTCGGGGCGTCACACAGGCTCGCTACACCGTTGAGGCGCTTCAGGCGCCGTAACGGAGAACA
CCCATGGCCTTCGCCCCCGG

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTDTAIETAELERRKAEARAWFEALQGRIIAAFEQLEDEASPALYGEKAGRFVRTPWDRTDHTGAPGGGGTMAMMHGRLF
EKVGVHTSTVFGEFAPEFRGQIPGAAEDPRFWASGISLIAHMANPHVPAVHMNTRFVVTTKSWFGGGADLTPVLDTRRTQ
DDPDTRAFHAAFEKACGAHAVADYGRYKAWCDEYFFLKHRNEMRGIGGIFYDWLNSGDFGADLAFTREVGTTFLDIYPRL
VRANMARPWDETEREEQLVRRGRYVEFNLLYDRGTIFGLKTGGNIASILSSMPPVVKWP

Sequences:

>Translated_299_residues
MTDTAIETAELERRKAEARAWFEALQGRIIAAFEQLEDEASPALYGEKAGRFVRTPWDRTDHTGAPGGGGTMAMMHGRLF
EKVGVHTSTVFGEFAPEFRGQIPGAAEDPRFWASGISLIAHMANPHVPAVHMNTRFVVTTKSWFGGGADLTPVLDTRRTQ
DDPDTRAFHAAFEKACGAHAVADYGRYKAWCDEYFFLKHRNEMRGIGGIFYDWLNSGDFGADLAFTREVGTTFLDIYPRL
VRANMARPWDETEREEQLVRRGRYVEFNLLYDRGTIFGLKTGGNIASILSSMPPVVKWP
>Mature_298_residues
TDTAIETAELERRKAEARAWFEALQGRIIAAFEQLEDEASPALYGEKAGRFVRTPWDRTDHTGAPGGGGTMAMMHGRLFE
KVGVHTSTVFGEFAPEFRGQIPGAAEDPRFWASGISLIAHMANPHVPAVHMNTRFVVTTKSWFGGGADLTPVLDTRRTQD
DPDTRAFHAAFEKACGAHAVADYGRYKAWCDEYFFLKHRNEMRGIGGIFYDWLNSGDFGADLAFTREVGTTFLDIYPRLV
RANMARPWDETEREEQLVRRGRYVEFNLLYDRGTIFGLKTGGNIASILSSMPPVVKWP

Specific function: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III [H]

COG id: COG0408

COG function: function code H; Coproporphyrinogen III oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI41393599, Length=316, Percent_Identity=34.8101265822785, Blast_Score=173, Evalue=2e-43,
Organism=Escherichia coli, GI1788777, Length=274, Percent_Identity=43.4306569343066, Blast_Score=204, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6320249, Length=299, Percent_Identity=36.7892976588629, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI17864388, Length=291, Percent_Identity=39.5189003436426, Blast_Score=191, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001260
- InterPro:   IPR018375 [H]

Pfam domain/function: PF01218 Coprogen_oxidas [H]

EC number: =1.3.3.3 [H]

Molecular weight: Translated: 33518; Mature: 33387

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS01021 COPROGEN_OXIDASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTAIETAELERRKAEARAWFEALQGRIIAAFEQLEDEASPALYGEKAGRFVRTPWDRT
CCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHCCCEEECCCCCC
DHTGAPGGGGTMAMMHGRLFEKVGVHTSTVFGEFAPEFRGQIPGAAEDPRFWASGISLIA
CCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHHH
HMANPHVPAVHMNTRFVVTTKSWFGGGADLTPVLDTRRTQDDPDTRAFHAAFEKACGAHA
HHCCCCCCEEEECCEEEEEEHHHCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCHHH
VADYGRYKAWCDEYFFLKHRNEMRGIGGIFYDWLNSGDFGADLAFTREVGTTFLDIYPRL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHH
VRANMARPWDETEREEQLVRRGRYVEFNLLYDRGTIFGLKTGGNIASILSSMPPVVKWP
HHHHCCCCCCCHHHHHHHHHCCCCEEEEEEEECCEEEEEECCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TDTAIETAELERRKAEARAWFEALQGRIIAAFEQLEDEASPALYGEKAGRFVRTPWDRT
CCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHCCCEEECCCCCC
DHTGAPGGGGTMAMMHGRLFEKVGVHTSTVFGEFAPEFRGQIPGAAEDPRFWASGISLIA
CCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHHH
HMANPHVPAVHMNTRFVVTTKSWFGGGADLTPVLDTRRTQDDPDTRAFHAAFEKACGAHA
HHCCCCCCEEEECCEEEEEEHHHCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCHHH
VADYGRYKAWCDEYFFLKHRNEMRGIGGIFYDWLNSGDFGADLAFTREVGTTFLDIYPRL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHH
VRANMARPWDETEREEQLVRRGRYVEFNLLYDRGTIFGLKTGGNIASILSSMPPVVKWP
HHHHCCCCCCCHHHHHHHHHCCCCEEEEEEEECCEEEEEECCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]