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The map label for this gene is trmL [H]

Identifier: 158425126

GI number: 158425126

Start: 4018966

End: 4019436

Strand: Direct

Name: trmL [H]

Synonym: AZC_3502

Alternate gene names: 158425126

Gene position: 4018966-4019436 (Clockwise)

Preceding gene: 158425122

Following gene: 158425127

Centisome position: 74.84

GC content: 70.7

Gene sequence:

>471_bases
ATGATCCTCGCTCTTTACTGCCCAGATATCGCTCAGAATACCGGCACGTGCCTGCGCACCGGCGCCTGCCTCGGTGTTCC
CGTGCACATCATCGAGCCCGCCGGCTTTCCGGTGAGCGCGCCCGCGTTCCGCCGCGCCGGCATGGACTATCTCGATGCGG
TGGAGTGGCAACGCCATGCTTCCTTCGCCGCCTTCGAGCGCTGGCGCCGCGAGGAAGGCCGGCGCCTCGTGCTCTTCACC
ACGAAGGGGGCGACGCCCCTGCCCGCCTTCACCTTCCAGCCCGGCGACGTGCTGCTGTTCGGCCGTGAGAGCGCGGGAGC
GCCGGAGGAGGTCCATGCGGCCGCGGATGCGCGGGTGAAGATCCCGCTGCGGCCGGGCATGCGCTCGCTGAACGTGGCGG
TCTCCGCCGCCATGGCGCTGGGCGAGGCCCTGCGGCAGACGAACGGTTTCCCCGCCCCGGAGCCCACATGA

Upstream 100 bases:

>100_bases
TACACCCTATCCGAACCGTGTCCAGATCGCCCCCATGCGGCACCGGGTCTCGGGTTCGCCATCTTTCCGCCAGAACTCTG
CCAGCTACGTAACTTTACGG

Downstream 100 bases:

>100_bases
TTCTCCGGCGCGGGTCCCTGCCCGCCGCTTCCGTCGGCGGACCGAACATGCTCTAGAACGCGCGGGGGCCCGCAGGAGGT
TAAGCGCCGATGACCGACAC

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MILALYCPDIAQNTGTCLRTGACLGVPVHIIEPAGFPVSAPAFRRAGMDYLDAVEWQRHASFAAFERWRREEGRRLVLFT
TKGATPLPAFTFQPGDVLLFGRESAGAPEEVHAAADARVKIPLRPGMRSLNVAVSAAMALGEALRQTNGFPAPEPT

Sequences:

>Translated_156_residues
MILALYCPDIAQNTGTCLRTGACLGVPVHIIEPAGFPVSAPAFRRAGMDYLDAVEWQRHASFAAFERWRREEGRRLVLFT
TKGATPLPAFTFQPGDVLLFGRESAGAPEEVHAAADARVKIPLRPGMRSLNVAVSAAMALGEALRQTNGFPAPEPT
>Mature_156_residues
MILALYCPDIAQNTGTCLRTGACLGVPVHIIEPAGFPVSAPAFRRAGMDYLDAVEWQRHASFAAFERWRREEGRRLVLFT
TKGATPLPAFTFQPGDVLLFGRESAGAPEEVHAAADARVKIPLRPGMRSLNVAVSAAMALGEALRQTNGFPAPEPT

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=151, Percent_Identity=38.4105960264901, Blast_Score=100, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 16817; Mature: 16817

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILALYCPDIAQNTGTCLRTGACLGVPVHIIEPAGFPVSAPAFRRAGMDYLDAVEWQRHA
CEEEEECCCHHCCCCCEEECCCCCCCCEEEECCCCCCCCCHHHHHCCCHHHHHHHHHHHH
SFAAFERWRREEGRRLVLFTTKGATPLPAFTFQPGDVLLFGRESAGAPEEVHAAADARVK
HHHHHHHHHHHCCCEEEEEEECCCCCCCEEEECCCCEEEECCCCCCCCHHHHHCCCCEEE
IPLRPGMRSLNVAVSAAMALGEALRQTNGFPAPEPT
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MILALYCPDIAQNTGTCLRTGACLGVPVHIIEPAGFPVSAPAFRRAGMDYLDAVEWQRHA
CEEEEECCCHHCCCCCEEECCCCCCCCEEEECCCCCCCCCHHHHHCCCHHHHHHHHHHHH
SFAAFERWRREEGRRLVLFTTKGATPLPAFTFQPGDVLLFGRESAGAPEEVHAAADARVK
HHHHHHHHHHHCCCEEEEEEECCCCCCCEEEECCCCEEEECCCCCCCCHHHHHCCCCEEE
IPLRPGMRSLNVAVSAAMALGEALRQTNGFPAPEPT
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA