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The map label for this gene is pyk [H]

Identifier: 158425115

GI number: 158425115

Start: 4008227

End: 4009702

Strand: Direct

Name: pyk [H]

Synonym: AZC_3491

Alternate gene names: 158425115

Gene position: 4008227-4009702 (Clockwise)

Preceding gene: 158425114

Following gene: 158425116

Centisome position: 74.64

GC content: 67.89

Gene sequence:

>1476_bases
ATGCCGCTGAAGCGCAACCGCGCCACGAAAATCATCGCCACCGTGGGCCCGGCCTCCAACACGCCGGAGAAGCTCAAGGC
CCTCTTCCTGGCCGGTGTCGATGTGTTCCGGCTCAATTTCAGCCATGGGACGCAGGCCGACCATGGCATCGTTCTCGGCC
ATATCCGCCGCCTTGAAGCCGAGATCGGCCGCCCCATCGGCGTCATCGCCGATCTTCAGGGCCCGAAGCTGCGCGTCGGC
CGCTTCACCGAGGGTGCCATCACCTTCACCCCCGGCAAGCGCCTGCGCTTCGACGCAGATGTCGCGACCATGGGCGATGC
CGACCGCGTGCCCATTCCCCACCCCGACATCATCGAAGCCCTGACCATCGGCAGCACCGTCCTGTGCGACGACGGCAAGG
TGCGCCTGAAGGTGGTGGGCAAGGGCGACGGCTGGCTCGACGCGGAAGTGGTCAGCGGCACGCGCCTGTCCAACAACAAG
GGCTTCAACATCCCCGACGTGGTGCTGCCGCTCTCCCCTCTGACGGAGAAGGACCGCGCCGACCTCGCCTTCGCCGTGGA
GCAGGGCGTGGAGTGGATCGCTCTGTCCTTCGTGCAGCGCCCGGAAGACATCCACGAGGCACGCGACCTCATCAAGGGCC
GCGCTGCCGTGATGCTGAAGATGGAGAAGCCGGCGGCCGTCGAGCATCTGGAAGAGCTTGTGGAGCTTTCCGATGCCATC
ATGGTGGCGCGCGGCGATCTCGGCGTGGAACTCTCGCTGCCCGAACTGCCCGCCCTCCAGAAGCGTATGATTACGGAATC
CCGCAGACAGGGTCGCCCGGTGATCGTGGCGACCCAGATGCTGGAGAGCATGATCTCCGCCCCCGTGCCCACCCGCGCGG
AGGTCTCCGACGTCGCCACCGCCGTCTATGAGGGCGCGGACTGCGTGATGCTCTCCGCCGAGAGCGCGGCGGGCCAGTAT
CCCGTGGAAGCGGTCTCCTTCATGGACGAAATCATCCGCCACGTGGAGCGCGACCCCGGCTACCGGCAGGTGCTGGATGC
GACGCAGGCGGAATGGGGCAAGACCATCGGCGATGCCATGACCAAGGCCGCCTACCAGACGGCGCTCGCCGTCGATGCCG
CCGCCATCGTCGCCTATACGCTCTCCGGCACCACGGGCCTGCGCGCCGCACGCGAGCGCCCGCCCATGCCCATCCTCGGC
ATCACAACCCGCGCCGACACCGCCCGCCGGCTCGCCCTCTCTTATGGCGTGCATGCGGTGAATGTGGGCGAGGACATCCA
TTCCTTCGGCGAGATGGTGGACCGCGCGGTGCAGGTGGCCGTGGAGCAGCAGCTGGCCGCGCCCGGCGATCGGCTCGCGA
TCACGGCCGGCGTGCCCTTCGCCAAGCCCGGCACCACGAACATCCTGCGGGTCACCACCATCGGGGATACCGACCCGCCG
TCGAAGTTCGACACGGACGGTGCGACCGCAACCTGA

Upstream 100 bases:

>100_bases
GGGCCATGGCGGTGACGCACCTGCGTCACCGCGCCTTTGTATCAAGTGCGATCAGGACAAAGGCGGCACGGCAGATGTCG
CCCAATCGGGGGAGACATCC

Downstream 100 bases:

>100_bases
AGCACGCAGGCGCCAGCGCACGCCCGCCCTGGTCGATAAAGCCGGCCCCTCGCGCCGCCTGACGTAAGGGCGATGCGACC
ATTTGCTGACCTCTTCAGGG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MPLKRNRATKIIATVGPASNTPEKLKALFLAGVDVFRLNFSHGTQADHGIVLGHIRRLEAEIGRPIGVIADLQGPKLRVG
RFTEGAITFTPGKRLRFDADVATMGDADRVPIPHPDIIEALTIGSTVLCDDGKVRLKVVGKGDGWLDAEVVSGTRLSNNK
GFNIPDVVLPLSPLTEKDRADLAFAVEQGVEWIALSFVQRPEDIHEARDLIKGRAAVMLKMEKPAAVEHLEELVELSDAI
MVARGDLGVELSLPELPALQKRMITESRRQGRPVIVATQMLESMISAPVPTRAEVSDVATAVYEGADCVMLSAESAAGQY
PVEAVSFMDEIIRHVERDPGYRQVLDATQAEWGKTIGDAMTKAAYQTALAVDAAAIVAYTLSGTTGLRAARERPPMPILG
ITTRADTARRLALSYGVHAVNVGEDIHSFGEMVDRAVQVAVEQQLAAPGDRLAITAGVPFAKPGTTNILRVTTIGDTDPP
SKFDTDGATAT

Sequences:

>Translated_491_residues
MPLKRNRATKIIATVGPASNTPEKLKALFLAGVDVFRLNFSHGTQADHGIVLGHIRRLEAEIGRPIGVIADLQGPKLRVG
RFTEGAITFTPGKRLRFDADVATMGDADRVPIPHPDIIEALTIGSTVLCDDGKVRLKVVGKGDGWLDAEVVSGTRLSNNK
GFNIPDVVLPLSPLTEKDRADLAFAVEQGVEWIALSFVQRPEDIHEARDLIKGRAAVMLKMEKPAAVEHLEELVELSDAI
MVARGDLGVELSLPELPALQKRMITESRRQGRPVIVATQMLESMISAPVPTRAEVSDVATAVYEGADCVMLSAESAAGQY
PVEAVSFMDEIIRHVERDPGYRQVLDATQAEWGKTIGDAMTKAAYQTALAVDAAAIVAYTLSGTTGLRAARERPPMPILG
ITTRADTARRLALSYGVHAVNVGEDIHSFGEMVDRAVQVAVEQQLAAPGDRLAITAGVPFAKPGTTNILRVTTIGDTDPP
SKFDTDGATAT
>Mature_490_residues
PLKRNRATKIIATVGPASNTPEKLKALFLAGVDVFRLNFSHGTQADHGIVLGHIRRLEAEIGRPIGVIADLQGPKLRVGR
FTEGAITFTPGKRLRFDADVATMGDADRVPIPHPDIIEALTIGSTVLCDDGKVRLKVVGKGDGWLDAEVVSGTRLSNNKG
FNIPDVVLPLSPLTEKDRADLAFAVEQGVEWIALSFVQRPEDIHEARDLIKGRAAVMLKMEKPAAVEHLEELVELSDAIM
VARGDLGVELSLPELPALQKRMITESRRQGRPVIVATQMLESMISAPVPTRAEVSDVATAVYEGADCVMLSAESAAGQYP
VEAVSFMDEIIRHVERDPGYRQVLDATQAEWGKTIGDAMTKAAYQTALAVDAAAIVAYTLSGTTGLRAARERPPMPILGI
TTRADTARRLALSYGVHAVNVGEDIHSFGEMVDRAVQVAVEQQLAAPGDRLAITAGVPFAKPGTTNILRVTTIGDTDPPS
KFDTDGATAT

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=492, Percent_Identity=36.1788617886179, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI33286420, Length=492, Percent_Identity=36.1788617886179, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI33286418, Length=492, Percent_Identity=36.1788617886179, Blast_Score=275, Evalue=1e-73,
Organism=Homo sapiens, GI32967597, Length=491, Percent_Identity=36.0488798370672, Blast_Score=270, Evalue=3e-72,
Organism=Homo sapiens, GI10835121, Length=491, Percent_Identity=36.0488798370672, Blast_Score=270, Evalue=3e-72,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=36.4583333333333, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=36.4583333333333, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=33.8983050847458, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=33.8983050847458, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=31.6279069767442, Blast_Score=108, Evalue=9e-24,
Organism=Escherichia coli, GI1787965, Length=471, Percent_Identity=35.6687898089172, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI1788160, Length=481, Percent_Identity=36.7983367983368, Blast_Score=280, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17544584, Length=484, Percent_Identity=33.4710743801653, Blast_Score=254, Evalue=5e-68,
Organism=Caenorhabditis elegans, GI71984406, Length=484, Percent_Identity=34.7107438016529, Blast_Score=249, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI17506829, Length=484, Percent_Identity=34.7107438016529, Blast_Score=249, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI71984413, Length=484, Percent_Identity=34.7107438016529, Blast_Score=249, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI17506831, Length=484, Percent_Identity=34.7107438016529, Blast_Score=248, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6319279, Length=485, Percent_Identity=33.8144329896907, Blast_Score=254, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6324923, Length=495, Percent_Identity=34.3434343434343, Blast_Score=253, Evalue=5e-68,
Organism=Drosophila melanogaster, GI24648964, Length=493, Percent_Identity=33.2657200811359, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI28571814, Length=493, Percent_Identity=33.2657200811359, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24581235, Length=424, Percent_Identity=31.1320754716981, Blast_Score=229, Evalue=5e-60,
Organism=Drosophila melanogaster, GI24648966, Length=409, Percent_Identity=32.7628361858191, Blast_Score=205, Evalue=7e-53,
Organism=Drosophila melanogaster, GI24646914, Length=265, Percent_Identity=33.5849056603774, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52633; Mature: 52502

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLKRNRATKIIATVGPASNTPEKLKALFLAGVDVFRLNFSHGTQADHGIVLGHIRRLEA
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHH
EIGRPIGVIADLQGPKLRVGRFTEGAITFTPGKRLRFDADVATMGDADRVPIPHPDIIEA
HHCCCEEEEEECCCCCEEECEEECCEEEECCCCCEEECCCEEECCCCCCCCCCCCHHHHH
LTIGSTVLCDDGKVRLKVVGKGDGWLDAEVVSGTRLSNNKGFNIPDVVLPLSPLTEKDRA
HHCCCEEEECCCCEEEEEEECCCCCEEEEEECCEEECCCCCCCCCCEEECCCCCCCCCCH
DLAFAVEQGVEWIALSFVQRPEDIHEARDLIKGRAAVMLKMEKPAAVEHLEELVELSDAI
HHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHE
MVARGDLGVELSLPELPALQKRMITESRRQGRPVIVATQMLESMISAPVPTRAEVSDVAT
EEEECCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCHHHHHHH
AVYEGADCVMLSAESAAGQYPVEAVSFMDEIIRHVERDPGYRQVLDATQAEWGKTIGDAM
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
TKAAYQTALAVDAAAIVAYTLSGTTGLRAARERPPMPILGITTRADTARRLALSYGVHAV
HHHHHHHHHHHHHHEEEEEEECCCCHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCEEEE
NVGEDIHSFGEMVDRAVQVAVEQQLAAPGDRLAITAGVPFAKPGTTNILRVTTIGDTDPP
ECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCC
SKFDTDGATAT
CCCCCCCCCCC
>Mature Secondary Structure 
PLKRNRATKIIATVGPASNTPEKLKALFLAGVDVFRLNFSHGTQADHGIVLGHIRRLEA
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHH
EIGRPIGVIADLQGPKLRVGRFTEGAITFTPGKRLRFDADVATMGDADRVPIPHPDIIEA
HHCCCEEEEEECCCCCEEECEEECCEEEECCCCCEEECCCEEECCCCCCCCCCCCHHHHH
LTIGSTVLCDDGKVRLKVVGKGDGWLDAEVVSGTRLSNNKGFNIPDVVLPLSPLTEKDRA
HHCCCEEEECCCCEEEEEEECCCCCEEEEEECCEEECCCCCCCCCCEEECCCCCCCCCCH
DLAFAVEQGVEWIALSFVQRPEDIHEARDLIKGRAAVMLKMEKPAAVEHLEELVELSDAI
HHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHE
MVARGDLGVELSLPELPALQKRMITESRRQGRPVIVATQMLESMISAPVPTRAEVSDVAT
EEEECCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCHHHHHHH
AVYEGADCVMLSAESAAGQYPVEAVSFMDEIIRHVERDPGYRQVLDATQAEWGKTIGDAM
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
TKAAYQTALAVDAAAIVAYTLSGTTGLRAARERPPMPILGITTRADTARRLALSYGVHAV
HHHHHHHHHHHHHHEEEEEEECCCCHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCEEEE
NVGEDIHSFGEMVDRAVQVAVEQQLAAPGDRLAITAGVPFAKPGTTNILRVTTIGDTDPP
ECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCC
SKFDTDGATAT
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA