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The map label for this gene is nuoF2 [H]

Identifier: 158425077

GI number: 158425077

Start: 3967581

End: 3969140

Strand: Direct

Name: nuoF2 [H]

Synonym: AZC_3453

Alternate gene names: 158425077

Gene position: 3967581-3969140 (Clockwise)

Preceding gene: 158425076

Following gene: 158425078

Centisome position: 73.89

GC content: 68.59

Gene sequence:

>1560_bases
ATGAGCGTGCGCATCTTCGTTCCCAACGACGCCACCGCCATCGCCTGCGGCGCCGACAAGGTGGCCCGCGCCATCGAGGC
GGGGCTGGCCGCCCGTGGCATCGTCGCCAACATCGTGCGCAACGGCTCGCGCGGCCTGTTCTGGCTGGAGCCCCTCGTCG
AGGTGGAGACACCGAAGGGCCGTGTCGGCTACGGCCCGGTGAAGCCGTCTGACGTGGAGAGCCTGCTGGCCGCCGGCCTC
GTCCACGGCGCGGACCATCCGCTGAGCGTCGGCCTCGTGGAGGAAATCCCCTTCCTCAAGAAGCAGACCCGACTCACCTT
CGCGCGCTGCGGCATCATCGACCCGCTGTCGCTGGAAGAGTACAAGGCCCACGGCGGCTACAAGGGCCTTGCCAAGGCCA
TCGAGATCGGCCCCTCGGCCACCGTCGAACAGGTGTTCGAGAGCGGCCTGCGCGGCCGTGGCGGCGCCGGCTTCCCGACA
GGCATCAAGTGGCGCACGGTGGCCGCCGCCCCCGCCGACCAGCGCTACATCGTCTGCAATGCGGACGAAGGCGACAGCGG
CACCTTCGCCGACCGTCTCATCATGGAGGCGGACCCCTTCTGCCTCATCGAGGGCATGACCATCGCCGGCCTCGCGGTGG
GCGCCACCAAGGGCTTCGTCTACTGCCGCTCGGAATATCCGCTTTCGCTGCAGGTGATGGAGAAGGCGATCGCCATCGCC
CGCACCGCCGGCCTGCTGGGCAAGAATGTCGCCGGCTCGCAGTATGACTTCGACCTCGAAATGCGCATGGGCGCGGGCGC
CTATGTGTGCGGCGAGGAAACGGCGCTGCTCGACAGCCTGGAAGGCAAGCGCGGCGTGGTCCGCGCCAAGCCCCCGCTGC
CGGCGCACAAGGGCCTGTTCGGCAAGCCCACCGTCATCAACAACCTGATCTCGCTCGCCACCGTGCCGGTGATCCTGGAG
AAGGGCGGCGCCTTCTACCGCGACTTCGGCATGGGCCGGTCGCGCGGCACCATGCCCATCCAGCTTGCCGGTAACATCAA
GTTCGGCGGCATCTTCGAGACCGCCTTCGGCATTCCGCTCGGCGAGCTCGTCTATGACATCGGCGGGGGCACCGCCTCCG
GTCGCCCAGTGCGCGCGGTGCAGGCAGGCGGCCCGCTCGGCGCCTATCTGCCGGTGCACCAGTTCGACACGCCGTTCGAT
TACGAGGCCTTCGCGGCCAAGGACGCGCTCATCGGCCATGGCGGCATCGTGGTGTTCGACGACACGGTGGACATGGCCCA
CATGGCCCGCTTCGCCATGGAGTTCTGCTCCCACGAGAGCTGCGGCAAGTGCACCCCCTGCCGCATCGGCTCCACGCGCG
GCGTGGAGACGCTGGACAAGATCATCGCCGGCAACCGGCGGGAGGCGAACCTCGCCGTCCTTACCGACCTTTGCCAGACC
ATGAAGCTCGGTTCGCTCTGCGCGCTCGGCGGCTTCACGCCCTATCCCGTGCTCAGTGCCCTCACCCATTTCCCGGAAGA
CTTCGGCGCCACGCCGAAGCTGCCCGCGGCCGCCGAATAG

Upstream 100 bases:

>100_bases
TCGGCCTGTGCGCCTGCGCCCCCTCGGCCATGATCGACGGCCGCCTCGTCGGTCGGCTCGACGAAGCCACCATCGACGAA
CTCGTGGCGGAGGCCGGACA

Downstream 100 bases:

>100_bases
GAGGAACGCCATGTCCCTCGTGCATGAAATCGACTACGGCACCCCGGCCTCCAAATCCGAGAAGCTGGTCACGCTCACCA
TCGACGGCATGAAGGTGCAG

Product: NAD-dependent formate dehydrogenase subunit beta protein

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MSVRIFVPNDATAIACGADKVARAIEAGLAARGIVANIVRNGSRGLFWLEPLVEVETPKGRVGYGPVKPSDVESLLAAGL
VHGADHPLSVGLVEEIPFLKKQTRLTFARCGIIDPLSLEEYKAHGGYKGLAKAIEIGPSATVEQVFESGLRGRGGAGFPT
GIKWRTVAAAPADQRYIVCNADEGDSGTFADRLIMEADPFCLIEGMTIAGLAVGATKGFVYCRSEYPLSLQVMEKAIAIA
RTAGLLGKNVAGSQYDFDLEMRMGAGAYVCGEETALLDSLEGKRGVVRAKPPLPAHKGLFGKPTVINNLISLATVPVILE
KGGAFYRDFGMGRSRGTMPIQLAGNIKFGGIFETAFGIPLGELVYDIGGGTASGRPVRAVQAGGPLGAYLPVHQFDTPFD
YEAFAAKDALIGHGGIVVFDDTVDMAHMARFAMEFCSHESCGKCTPCRIGSTRGVETLDKIIAGNRREANLAVLTDLCQT
MKLGSLCALGGFTPYPVLSALTHFPEDFGATPKLPAAAE

Sequences:

>Translated_519_residues
MSVRIFVPNDATAIACGADKVARAIEAGLAARGIVANIVRNGSRGLFWLEPLVEVETPKGRVGYGPVKPSDVESLLAAGL
VHGADHPLSVGLVEEIPFLKKQTRLTFARCGIIDPLSLEEYKAHGGYKGLAKAIEIGPSATVEQVFESGLRGRGGAGFPT
GIKWRTVAAAPADQRYIVCNADEGDSGTFADRLIMEADPFCLIEGMTIAGLAVGATKGFVYCRSEYPLSLQVMEKAIAIA
RTAGLLGKNVAGSQYDFDLEMRMGAGAYVCGEETALLDSLEGKRGVVRAKPPLPAHKGLFGKPTVINNLISLATVPVILE
KGGAFYRDFGMGRSRGTMPIQLAGNIKFGGIFETAFGIPLGELVYDIGGGTASGRPVRAVQAGGPLGAYLPVHQFDTPFD
YEAFAAKDALIGHGGIVVFDDTVDMAHMARFAMEFCSHESCGKCTPCRIGSTRGVETLDKIIAGNRREANLAVLTDLCQT
MKLGSLCALGGFTPYPVLSALTHFPEDFGATPKLPAAAE
>Mature_518_residues
SVRIFVPNDATAIACGADKVARAIEAGLAARGIVANIVRNGSRGLFWLEPLVEVETPKGRVGYGPVKPSDVESLLAAGLV
HGADHPLSVGLVEEIPFLKKQTRLTFARCGIIDPLSLEEYKAHGGYKGLAKAIEIGPSATVEQVFESGLRGRGGAGFPTG
IKWRTVAAAPADQRYIVCNADEGDSGTFADRLIMEADPFCLIEGMTIAGLAVGATKGFVYCRSEYPLSLQVMEKAIAIAR
TAGLLGKNVAGSQYDFDLEMRMGAGAYVCGEETALLDSLEGKRGVVRAKPPLPAHKGLFGKPTVINNLISLATVPVILEK
GGAFYRDFGMGRSRGTMPIQLAGNIKFGGIFETAFGIPLGELVYDIGGGTASGRPVRAVQAGGPLGAYLPVHQFDTPFDY
EAFAAKDALIGHGGIVVFDDTVDMAHMARFAMEFCSHESCGKCTPCRIGSTRGVETLDKIIAGNRREANLAVLTDLCQTM
KLGSLCALGGFTPYPVLSALTHFPEDFGATPKLPAAAE

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=389, Percent_Identity=38.0462724935733, Blast_Score=265, Evalue=7e-71,
Organism=Homo sapiens, GI20149568, Length=389, Percent_Identity=38.0462724935733, Blast_Score=265, Evalue=7e-71,
Organism=Escherichia coli, GI1788620, Length=408, Percent_Identity=39.2156862745098, Blast_Score=298, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI17531727, Length=390, Percent_Identity=38.2051282051282, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI19920794, Length=375, Percent_Identity=40.2666666666667, Blast_Score=270, Evalue=3e-72,
Organism=Drosophila melanogaster, GI45550452, Length=384, Percent_Identity=36.1979166666667, Blast_Score=248, Evalue=8e-66,
Organism=Drosophila melanogaster, GI24653819, Length=345, Percent_Identity=31.5942028985507, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24653817, Length=307, Percent_Identity=28.9902280130293, Blast_Score=140, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 54715; Mature: 54584

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRIFVPNDATAIACGADKVARAIEAGLAARGIVANIVRNGSRGLFWLEPLVEVETPKG
CEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEECCCC
RVGYGPVKPSDVESLLAAGLVHGADHPLSVGLVEEIPFLKKQTRLTFARCGIIDPLSLEE
CCCCCCCCCHHHHHHHHHHHHCCCCCCEEECHHHHCCHHHHHHHHHHHHCCCCCCCCHHH
YKAHGGYKGLAKAIEIGPSATVEQVFESGLRGRGGAGFPTGIKWRTVAAAPADQRYIVCN
HHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEE
ADEGDSGTFADRLIMEADPFCLIEGMTIAGLAVGATKGFVYCRSEYPLSLQVMEKAIAIA
CCCCCCCCHHHHHHHCCCCEEEEECCEEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHH
RTAGLLGKNVAGSQYDFDLEMRMGAGAYVCGEETALLDSLEGKRGVVRAKPPLPAHKGLF
HHHHHHCCCCCCCCCCEEEEEECCCCEEEECCCHHHHHHCCCCCCEEECCCCCCCCCCCC
GKPTVINNLISLATVPVILEKGGAFYRDFGMGRSRGTMPIQLAGNIKFGGIFETAFGIPL
CCCHHHHHHHHHHHHHHHEECCCCEEECCCCCCCCCCEEEEEECCEEECCEEHHHHCCCH
GELVYDIGGGTASGRPVRAVQAGGPLGAYLPVHQFDTPFDYEAFAAKDALIGHGGIVVFD
HHHHHCCCCCCCCCCCEEEEECCCCCCCCCCHHCCCCCCCCHHHHHCCCEEECCCEEEEE
DTVDMAHMARFAMEFCSHESCGKCTPCRIGSTRGVETLDKIIAGNRREANLAVLTDLCQT
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHH
MKLGSLCALGGFTPYPVLSALTHFPEDFGATPKLPAAAE
HHHCCCHHCCCCCHHHHHHHHHHCCHHHCCCCCCCCCCC
>Mature Secondary Structure 
SVRIFVPNDATAIACGADKVARAIEAGLAARGIVANIVRNGSRGLFWLEPLVEVETPKG
EEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEECCCC
RVGYGPVKPSDVESLLAAGLVHGADHPLSVGLVEEIPFLKKQTRLTFARCGIIDPLSLEE
CCCCCCCCCHHHHHHHHHHHHCCCCCCEEECHHHHCCHHHHHHHHHHHHCCCCCCCCHHH
YKAHGGYKGLAKAIEIGPSATVEQVFESGLRGRGGAGFPTGIKWRTVAAAPADQRYIVCN
HHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEE
ADEGDSGTFADRLIMEADPFCLIEGMTIAGLAVGATKGFVYCRSEYPLSLQVMEKAIAIA
CCCCCCCCHHHHHHHCCCCEEEEECCEEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHH
RTAGLLGKNVAGSQYDFDLEMRMGAGAYVCGEETALLDSLEGKRGVVRAKPPLPAHKGLF
HHHHHHCCCCCCCCCCEEEEEECCCCEEEECCCHHHHHHCCCCCCEEECCCCCCCCCCCC
GKPTVINNLISLATVPVILEKGGAFYRDFGMGRSRGTMPIQLAGNIKFGGIFETAFGIPL
CCCHHHHHHHHHHHHHHHEECCCCEEECCCCCCCCCCEEEEEECCEEECCEEHHHHCCCH
GELVYDIGGGTASGRPVRAVQAGGPLGAYLPVHQFDTPFDYEAFAAKDALIGHGGIVVFD
HHHHHCCCCCCCCCCCEEEEECCCCCCCCCCHHCCCCCCCCHHHHHCCCEEECCCEEEEE
DTVDMAHMARFAMEFCSHESCGKCTPCRIGSTRGVETLDKIIAGNRREANLAVLTDLCQT
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHH
MKLGSLCALGGFTPYPVLSALTHFPEDFGATPKLPAAAE
HHHCCCHHCCCCCHHHHHHHHHHCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]