Click here to switch to the map view.

The map label for this gene is nuoE [H]

Identifier: 158425076

GI number: 158425076

Start: 3967105

End: 3967584

Strand: Direct

Name: nuoE [H]

Synonym: AZC_3452

Alternate gene names: 158425076

Gene position: 3967105-3967584 (Clockwise)

Preceding gene: 158425075

Following gene: 158425077

Centisome position: 73.88

GC content: 67.71

Gene sequence:

>480_bases
TTGCCGATGGCCGGCTACGAACCATGGAGCGCCTCGCGCGCAGCGGAGATCATCGCCGAGCACCGGCACATGGATGGCGC
CACCATGCCCATCCTCCACGCGGTGCAGGAGACCTTCGGCTTCGTCCCCGACCCTGTCGTGCCCATGATCGCGGAGAGCC
TGAACCTCTCCCGCGCCGAGGTCTATGGCGTCGTCACCTTCTACCATGACTTCCGGCGCGAGCCGCCGGGCCGCCATGTC
ATCAAGCTCTGCGCGGCCGAAGCCTGCCAGTCCATGGGCTCGGAAAAGCTCGCCCGCTATGCGGAAGAGCGCCTCGGCAT
CGAGATGGGCGAGACCTCGCCGGACGGAAAGGTGACGCTGGAGCCCATCTACTGCCTCGGCCTGTGCGCCTGCGCCCCCT
CGGCCATGATCGACGGCCGCCTCGTCGGTCGGCTCGACGAAGCCACCATCGACGAACTCGTGGCGGAGGCCGGACAATGA

Upstream 100 bases:

>100_bases
TATCGCAACATCCCACAGCTGGCTTGATCCGCCCTGCCCCTGTGACACATTCATTGGAACGATCACAAGGAAACCGCTTC
AGGCGGGACGGGGAAGGGCA

Downstream 100 bases:

>100_bases
GCGTGCGCATCTTCGTTCCCAACGACGCCACCGCCATCGCCTGCGGCGCCGACAAGGTGGCCCGCGCCATCGAGGCGGGG
CTGGCCGCCCGTGGCATCGT

Product: NADH dehydrogenase I chain E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MPMAGYEPWSASRAAEIIAEHRHMDGATMPILHAVQETFGFVPDPVVPMIAESLNLSRAEVYGVVTFYHDFRREPPGRHV
IKLCAAEACQSMGSEKLARYAEERLGIEMGETSPDGKVTLEPIYCLGLCACAPSAMIDGRLVGRLDEATIDELVAEAGQ

Sequences:

>Translated_159_residues
MPMAGYEPWSASRAAEIIAEHRHMDGATMPILHAVQETFGFVPDPVVPMIAESLNLSRAEVYGVVTFYHDFRREPPGRHV
IKLCAAEACQSMGSEKLARYAEERLGIEMGETSPDGKVTLEPIYCLGLCACAPSAMIDGRLVGRLDEATIDELVAEAGQ
>Mature_158_residues
PMAGYEPWSASRAAEIIAEHRHMDGATMPILHAVQETFGFVPDPVVPMIAESLNLSRAEVYGVVTFYHDFRREPPGRHVI
KLCAAEACQSMGSEKLARYAEERLGIEMGETSPDGKVTLEPIYCLGLCACAPSAMIDGRLVGRLDEATIDELVAEAGQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=153, Percent_Identity=30.718954248366, Blast_Score=88, Evalue=3e-18,
Organism=Escherichia coli, GI1788621, Length=152, Percent_Identity=36.1842105263158, Blast_Score=92, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17561328, Length=132, Percent_Identity=34.0909090909091, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI18859877, Length=134, Percent_Identity=32.089552238806, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21355353, Length=131, Percent_Identity=28.2442748091603, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17307; Mature: 17176

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMAGYEPWSASRAAEIIAEHRHMDGATMPILHAVQETFGFVPDPVVPMIAESLNLSRAE
CCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHH
VYGVVTFYHDFRREPPGRHVIKLCAAEACQSMGSEKLARYAEERLGIEMGETSPDGKVTL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEE
EPIYCLGLCACAPSAMIDGRLVGRLDEATIDELVAEAGQ
HHHHHHHHHHHCCHHHHCCHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PMAGYEPWSASRAAEIIAEHRHMDGATMPILHAVQETFGFVPDPVVPMIAESLNLSRAE
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHH
VYGVVTFYHDFRREPPGRHVIKLCAAEACQSMGSEKLARYAEERLGIEMGETSPDGKVTL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEE
EPIYCLGLCACAPSAMIDGRLVGRLDEATIDELVAEAGQ
HHHHHHHHHHHCCHHHHCCHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]