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The map label for this gene is dctA2 [H]

Identifier: 158424977

GI number: 158424977

Start: 3881864

End: 3883186

Strand: Reverse

Name: dctA2 [H]

Synonym: AZC_3353

Alternate gene names: 158424977

Gene position: 3883186-3881864 (Counterclockwise)

Preceding gene: 158424978

Following gene: 158424976

Centisome position: 72.32

GC content: 64.1

Gene sequence:

>1323_bases
ATGTCCGTCACGCATGCCGCAACGCCGGTCAAGGCACCGGCCAAGACCCGGCCGTGGTATGCCCAACTCTGGGTGCAGGT
GATCGCCGCCATGATCATCGGCATCCTCGTGGGGCATTTCTACCCGCAGTTCGGCACCAAGATGCAGCCGCTGGGAGATG
CCTTCATCAAGGCCATCCGCATGCTCATCGCGCCCATCATCTTCTGTACGGTGGTGCACGGCATCGCGAAGATGGCGGAC
ATGGCCCGGGTCGGCCGCGTTGCGATCAAGTCGCTGATCTATTTCGAGATCATCACCACCATCGCGCTCATCATCGGGCT
GGTGATGGTCAATCTGTGGAAGCCCGGCGTCGGCATGAACGTGGACCTGTCCACGGTCGATACCACCTCCATCAAGGGCT
ATCTCGCCCAGACGGAGAAGACCGACACGATCTCCTTCCTCATGAGCATCATCCCCAACACCTTCCTCGGCGCCTTTGCC
GAGGGGCACGTCCTGCAGGTGCTGTTCCTCTCGGTGCTGTGCGGCTTCGGCCTCACCTGGATCGGCCCGCGCGGCAAGAT
GGTGATCGATCTCACCGAGACGGTGTCGCAGATGCTGTTCCAGGTGGTGGGCATCGTCATGTGGGCGGCACCGCTCGGCG
CGTTCGGGGCCATCGCCTTCACGGTGGGCAAGTTCGGCGCCACCTCGCTGCTGTCGCTCGGCAACCTCCTCATCTGCTTC
TACGTCACCTGCCTCATCTTCGTCGTGGTGGTGCTGGGCGCAGTCTCGCGGCTCTGCGGCTTCAGCCTCTTCAAGCTGCT
GCGCTACATCCGCGAGGAGATGCTCATCGTCTTCGCCACCACCTCGTCGGAGACGGTGCTGCCGCGCATGCTGGTGAAGA
TGGAGAAGGCCGGCTGCGATGAAAGCGTCGTCGGTCTCGTCATCCCCACCGGCTATTCGTTCAATCTCGACGGCACCTGC
CTCTATCTCGCGACGGCGGCCGTGTTCCTGGCGCAGGCCACCAACACGCCTCTCGATATCAGCCACCAGATCGGCCTGCT
TCTGATCCTGCTGCTCACCTCCAAGGGCGCCGCAGGCATTGCGGGTGCGGCCTTCGTGGTACTGGCGGCGACGCTTGCCT
CCTCCGGCACCATTCCGGTGGCGAGTGTCGCGCTCATCCTCGGCATCCACCGGCTCATGTCGGAAGGGCTCACACCCACC
AACCTCATCGGCAATGCGGTGGCGACCATCGCCATCGCCAAGTGGGAGAACGCGCTCGACGAGGACCGGCTGCGCCGCGT
GCTCGATGGCGAGGACGTCGAGGCGGCCTCTCCCGCGCGCTGA

Upstream 100 bases:

>100_bases
CGAGCCGGAGCTCCAGCACAAGGTGCTGGTGGAGAACCCGGCCCGCCTCTTCGATTTCAACGACGCCTGAGCCAACAGGC
GCTGACCGGGAGACGCCCCA

Downstream 100 bases:

>100_bases
TGCCCGCCGTCCTGCCGGGCGCTCTGTCCGGCCCAGCAGGACGTCCGGCTCCATTCCGCCGCAAGGCGCCACCGCCTGCG
GCCCTTTCAAGATGGCAATG

Product: C4-dicarboxylate transporter

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSVTHAATPVKAPAKTRPWYAQLWVQVIAAMIIGILVGHFYPQFGTKMQPLGDAFIKAIRMLIAPIIFCTVVHGIAKMAD
MARVGRVAIKSLIYFEIITTIALIIGLVMVNLWKPGVGMNVDLSTVDTTSIKGYLAQTEKTDTISFLMSIIPNTFLGAFA
EGHVLQVLFLSVLCGFGLTWIGPRGKMVIDLTETVSQMLFQVVGIVMWAAPLGAFGAIAFTVGKFGATSLLSLGNLLICF
YVTCLIFVVVVLGAVSRLCGFSLFKLLRYIREEMLIVFATTSSETVLPRMLVKMEKAGCDESVVGLVIPTGYSFNLDGTC
LYLATAAVFLAQATNTPLDISHQIGLLLILLLTSKGAAGIAGAAFVVLAATLASSGTIPVASVALILGIHRLMSEGLTPT
NLIGNAVATIAIAKWENALDEDRLRRVLDGEDVEAASPAR

Sequences:

>Translated_440_residues
MSVTHAATPVKAPAKTRPWYAQLWVQVIAAMIIGILVGHFYPQFGTKMQPLGDAFIKAIRMLIAPIIFCTVVHGIAKMAD
MARVGRVAIKSLIYFEIITTIALIIGLVMVNLWKPGVGMNVDLSTVDTTSIKGYLAQTEKTDTISFLMSIIPNTFLGAFA
EGHVLQVLFLSVLCGFGLTWIGPRGKMVIDLTETVSQMLFQVVGIVMWAAPLGAFGAIAFTVGKFGATSLLSLGNLLICF
YVTCLIFVVVVLGAVSRLCGFSLFKLLRYIREEMLIVFATTSSETVLPRMLVKMEKAGCDESVVGLVIPTGYSFNLDGTC
LYLATAAVFLAQATNTPLDISHQIGLLLILLLTSKGAAGIAGAAFVVLAATLASSGTIPVASVALILGIHRLMSEGLTPT
NLIGNAVATIAIAKWENALDEDRLRRVLDGEDVEAASPAR
>Mature_439_residues
SVTHAATPVKAPAKTRPWYAQLWVQVIAAMIIGILVGHFYPQFGTKMQPLGDAFIKAIRMLIAPIIFCTVVHGIAKMADM
ARVGRVAIKSLIYFEIITTIALIIGLVMVNLWKPGVGMNVDLSTVDTTSIKGYLAQTEKTDTISFLMSIIPNTFLGAFAE
GHVLQVLFLSVLCGFGLTWIGPRGKMVIDLTETVSQMLFQVVGIVMWAAPLGAFGAIAFTVGKFGATSLLSLGNLLICFY
VTCLIFVVVVLGAVSRLCGFSLFKLLRYIREEMLIVFATTSSETVLPRMLVKMEKAGCDESVVGLVIPTGYSFNLDGTCL
YLATAAVFLAQATNTPLDISHQIGLLLILLLTSKGAAGIAGAAFVVLAATLASSGTIPVASVALILGIHRLMSEGLTPTN
LIGNAVATIAIAKWENALDEDRLRRVLDGEDVEAASPAR

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI4827012, Length=436, Percent_Identity=25.6880733944954, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI40254478, Length=444, Percent_Identity=24.7747747747748, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI21314632, Length=443, Percent_Identity=23.7020316027088, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI66773030, Length=505, Percent_Identity=24.1584158415842, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI169790839, Length=439, Percent_Identity=24.373576309795, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI223468566, Length=257, Percent_Identity=28.0155642023346, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI194239697, Length=253, Percent_Identity=28.8537549407115, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI223468564, Length=249, Percent_Identity=27.710843373494, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI5032093, Length=374, Percent_Identity=24.331550802139, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1789947, Length=423, Percent_Identity=56.0283687943262, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI1790514, Length=412, Percent_Identity=40.0485436893204, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI1788024, Length=403, Percent_Identity=22.8287841191067, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789473, Length=329, Percent_Identity=24.6200607902736, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17537407, Length=396, Percent_Identity=25.2525252525253, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71983099, Length=460, Percent_Identity=24.3478260869565, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71983106, Length=460, Percent_Identity=24.3478260869565, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71996953, Length=427, Percent_Identity=26.6978922716628, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI193206654, Length=485, Percent_Identity=21.4432989690722, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI193206505, Length=421, Percent_Identity=22.8028503562945, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17541374, Length=403, Percent_Identity=23.3250620347395, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24583025, Length=428, Percent_Identity=25.7009345794392, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI17137668, Length=428, Percent_Identity=25.7009345794392, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24583023, Length=428, Percent_Identity=25.7009345794392, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI281360481, Length=420, Percent_Identity=24.5238095238095, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281360483, Length=420, Percent_Identity=24.5238095238095, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17137666, Length=420, Percent_Identity=24.5238095238095, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 46958; Mature: 46826

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SVTHAATPVKAPAKTRPWYAQLWVQVIAAMIIGILVGHFYPQFGTKMQPLGDAFIKAIR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH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HHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA