Click here to switch to the map view.

The map label for this gene is agaF [H]

Identifier: 158424663

GI number: 158424663

Start: 3470681

End: 3472753

Strand: Direct

Name: agaF [H]

Synonym: AZC_3039

Alternate gene names: 158424663

Gene position: 3470681-3472753 (Clockwise)

Preceding gene: 158424659

Following gene: 158424664

Centisome position: 64.63

GC content: 69.32

Gene sequence:

>2073_bases
ATGGCTCGCTGGCGCGTCGGTGTGGATTCGGGCGGAACGTTCACGGACATCTGCCTGTTCGACGAGGACGAGGGACGGGT
GGAGGTCTGGAAGGTCTCTTCCACCCCTGATGACCCCTCCCGCGGCATTGCTGAAGGTGTCGAGCAGGGCATGCGCCGCG
TCGCGCCTGAGAATGGCGACAAGCCGGCCTCCTCCGTCGCCTATTTCGGCCACGGCACGACGGTTGCCACCAATGCGCTG
ATCCAGCACCGGGGCGTCGCCACGGGCCTCGTCACCACGGACGGTTTCCGCGACCTGCTCGAGATCGGCCGCCAGAAGCG
CCCCGATCTCTATGACCTCACCGCCGACAAGCCGCCGACCCTGGTGCCGCGCGCCCTGCGCATGGCAGTGGGCGAGCGGG
TGCGCCATGACGGCTCCATCGACCTGCCGCTTGATGAGCCGGCGGTGCGCGAAGCCGCCAAGGCGCTGAAGAAGGCCGGC
GTGAAGGCCATCGCCGTCTCCTTCCTCTATGGCTTCGTCCGCCCCGAGCACGAGGCCCGCGCGGTGGAGATCATCCGCGA
GGAATATCCGGAGGCCTTCGTCTGCTCCGGCCACGAGATCGCCCCCGAATTCCGCGAGTTCGAGCGCCTCTCCACCGTGG
TGCTGAACGCCTATCTCGGCCCGGTGATGAAGTCCTATATCGAGCGCCTCTCCCCGCGCCTCGAGGCGCTGGGCATGGAG
GCGACGCCGCACCTCACCCAGTCCAACGGCGGCGTCATCGGCTTCTCCACCGCCGCCGCCATGCCGGTGCGCACCATCCT
CTCCGGCCCTTCCACCGGCGTCGTCGCGGCGCAGGCGGTGGGCGAACTGGCGGGCTTCCCCGACATCATCACCTTCGACA
TGGGCGGCACGTCCACGGACGTCGCTCTGTTGAAGGGCGGCGTGTGCAAGCTCACCTCGGAAGCCAATGTCCATGGCTAT
CCGCTGAAGGCCCCCATGCTGGACATCCACACGGTGGGCGCCGGCGGCGGCTCCATTGCCTATGTGGACTCCGGCGGCCT
CTTGAAGGTCGGCCCGCGCTCGTGCGGCGCCTTCCCCGGCCCGGTCTGCTACGACAAGGGCAATGAGGAGCCCGCCACCA
CCGACGCAAACGTGGTGCTCCAGACGCTGAACCCCACCCACCTGCTCGCCGGCCGCATGCCGGTGCGCCAGGATCTCGCC
AAGGCCGCCATCGGCCGCCTCGCCGACAAGCTCGGCCTCGGCGTCATGGAGACGGCGCAGGGCATCCTCTCCGTCGTCAC
CGCCAACATGGCCAAAGCGATCCGCGTCATCTCCGTGCAGCGCGGCCACGATCCGCGCGACTATACGCTGATGGCCTTCG
GCGGCGCCGGCCCTCTGCACGCCGCCCGGCTCGCCAAGGAGCTGGACATGCGCCGCGTGCTGGTGCCCCGAAATCCCGGC
ATCCTGTGCGCCATGGGCCTGCTGCTGACCGACCTGCGCGCGGATTTCGCGGCCACGAAGCTGCTGCCGCTCGCGGCCTC
CTCCCTCGCCCCGGTGAGCGAAGCCTTCGCCACCCTCAGCGCACGGGCGCAGGCCTGGTTCGACCATGAGGAGATCACCG
CCGCGGCGCGGCAGATGCACCGCACGGTGGACATGCGCTATGCGGGGCAGAATTACGAACTGTCCGTCGCCTTGCCGGAA
GGCGCCGTCACCGAGACCACGCTGGAGCATCTGGCGGAAGCCTTCGAGGAAGCCCACCGCCAGCGCTACGGCTTCATCGC
GGCAGGCGAAGCGGTGCAGATCGTCACCCTGCGCGTCGAGGCCGTCGGCCTCGTGAGGAAGGCGACGCTCAAGGCCTACC
CGCTGGAGGGGCCGGACGCCTCCGCCGCCATCATCGGCAAGCGGCCGGTGTGGTTCCCCGAGGCGGGCGACTACGTGCCA
ACCCCCATCTATGACCGGGAGGCGCTCAAAGCGGGCAACCGCTTCGCCGGCCCCGCCATCGTCGAGCAGATGGACACCAC
CACCGTCGTCCCGCCGGGCATGACCGCCCGCGTGGACGCCTATCACAACCTCATCCTCGAGGTGGCGGCATGA

Upstream 100 bases:

>100_bases
GGACTGGAAACGTGCACATTGAATGGAATTTTTACGCTTGCGAGGCAAGCCATGCAGGGGGAGGCTGAAGCCATAGACAC
GGTAATTTTGGGATCCGGTC

Downstream 100 bases:

>100_bases
CCCTGAAGCCCTCTCTCGCGCCCTCCGCCGCGCCGGCCCTTGATCCGATCACCGTCGAGGTGATCGGGGCCGCCCTCTCC
TCCATCGTGGAAGAGACCGG

Product: AgaF protein

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 690; Mature: 689

Protein sequence:

>690_residues
MARWRVGVDSGGTFTDICLFDEDEGRVEVWKVSSTPDDPSRGIAEGVEQGMRRVAPENGDKPASSVAYFGHGTTVATNAL
IQHRGVATGLVTTDGFRDLLEIGRQKRPDLYDLTADKPPTLVPRALRMAVGERVRHDGSIDLPLDEPAVREAAKALKKAG
VKAIAVSFLYGFVRPEHEARAVEIIREEYPEAFVCSGHEIAPEFREFERLSTVVLNAYLGPVMKSYIERLSPRLEALGME
ATPHLTQSNGGVIGFSTAAAMPVRTILSGPSTGVVAAQAVGELAGFPDIITFDMGGTSTDVALLKGGVCKLTSEANVHGY
PLKAPMLDIHTVGAGGGSIAYVDSGGLLKVGPRSCGAFPGPVCYDKGNEEPATTDANVVLQTLNPTHLLAGRMPVRQDLA
KAAIGRLADKLGLGVMETAQGILSVVTANMAKAIRVISVQRGHDPRDYTLMAFGGAGPLHAARLAKELDMRRVLVPRNPG
ILCAMGLLLTDLRADFAATKLLPLAASSLAPVSEAFATLSARAQAWFDHEEITAAARQMHRTVDMRYAGQNYELSVALPE
GAVTETTLEHLAEAFEEAHRQRYGFIAAGEAVQIVTLRVEAVGLVRKATLKAYPLEGPDASAAIIGKRPVWFPEAGDYVP
TPIYDREALKAGNRFAGPAIVEQMDTTTVVPPGMTARVDAYHNLILEVAA

Sequences:

>Translated_690_residues
MARWRVGVDSGGTFTDICLFDEDEGRVEVWKVSSTPDDPSRGIAEGVEQGMRRVAPENGDKPASSVAYFGHGTTVATNAL
IQHRGVATGLVTTDGFRDLLEIGRQKRPDLYDLTADKPPTLVPRALRMAVGERVRHDGSIDLPLDEPAVREAAKALKKAG
VKAIAVSFLYGFVRPEHEARAVEIIREEYPEAFVCSGHEIAPEFREFERLSTVVLNAYLGPVMKSYIERLSPRLEALGME
ATPHLTQSNGGVIGFSTAAAMPVRTILSGPSTGVVAAQAVGELAGFPDIITFDMGGTSTDVALLKGGVCKLTSEANVHGY
PLKAPMLDIHTVGAGGGSIAYVDSGGLLKVGPRSCGAFPGPVCYDKGNEEPATTDANVVLQTLNPTHLLAGRMPVRQDLA
KAAIGRLADKLGLGVMETAQGILSVVTANMAKAIRVISVQRGHDPRDYTLMAFGGAGPLHAARLAKELDMRRVLVPRNPG
ILCAMGLLLTDLRADFAATKLLPLAASSLAPVSEAFATLSARAQAWFDHEEITAAARQMHRTVDMRYAGQNYELSVALPE
GAVTETTLEHLAEAFEEAHRQRYGFIAAGEAVQIVTLRVEAVGLVRKATLKAYPLEGPDASAAIIGKRPVWFPEAGDYVP
TPIYDREALKAGNRFAGPAIVEQMDTTTVVPPGMTARVDAYHNLILEVAA
>Mature_689_residues
ARWRVGVDSGGTFTDICLFDEDEGRVEVWKVSSTPDDPSRGIAEGVEQGMRRVAPENGDKPASSVAYFGHGTTVATNALI
QHRGVATGLVTTDGFRDLLEIGRQKRPDLYDLTADKPPTLVPRALRMAVGERVRHDGSIDLPLDEPAVREAAKALKKAGV
KAIAVSFLYGFVRPEHEARAVEIIREEYPEAFVCSGHEIAPEFREFERLSTVVLNAYLGPVMKSYIERLSPRLEALGMEA
TPHLTQSNGGVIGFSTAAAMPVRTILSGPSTGVVAAQAVGELAGFPDIITFDMGGTSTDVALLKGGVCKLTSEANVHGYP
LKAPMLDIHTVGAGGGSIAYVDSGGLLKVGPRSCGAFPGPVCYDKGNEEPATTDANVVLQTLNPTHLLAGRMPVRQDLAK
AAIGRLADKLGLGVMETAQGILSVVTANMAKAIRVISVQRGHDPRDYTLMAFGGAGPLHAARLAKELDMRRVLVPRNPGI
LCAMGLLLTDLRADFAATKLLPLAASSLAPVSEAFATLSARAQAWFDHEEITAAARQMHRTVDMRYAGQNYELSVALPEG
AVTETTLEHLAEAFEEAHRQRYGFIAAGEAVQIVTLRVEAVGLVRKATLKAYPLEGPDASAAIIGKRPVWFPEAGDYVPT
PIYDREALKAGNRFAGPAIVEQMDTTTVVPPGMTARVDAYHNLILEVAA

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0145

COG function: function code EQ; N-methylhydantoinase A/acetone carboxylase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=731, Percent_Identity=30.2325581395349, Blast_Score=241, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI133901900, Length=706, Percent_Identity=27.3371104815864, Blast_Score=240, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6322634, Length=739, Percent_Identity=30.1759133964817, Blast_Score=237, Evalue=4e-63,
Organism=Drosophila melanogaster, GI45550492, Length=736, Percent_Identity=29.6195652173913, Blast_Score=267, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008040
- InterPro:   IPR002821 [H]

Pfam domain/function: PF05378 Hydant_A_N; PF01968 Hydantoinase_A [H]

EC number: 3.5.2.14

Molecular weight: Translated: 73619; Mature: 73488

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARWRVGVDSGGTFTDICLFDEDEGRVEVWKVSSTPDDPSRGIAEGVEQGMRRVAPENGD
CCEEEEECCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCC
KPASSVAYFGHGTTVATNALIQHRGVATGLVTTDGFRDLLEIGRQKRPDLYDLTADKPPT
CCCCCEEEECCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCEECCCCCCCC
LVPRALRMAVGERVRHDGSIDLPLDEPAVREAAKALKKAGVKAIAVSFLYGFVRPEHEAR
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHH
AVEIIREEYPEAFVCSGHEIAPEFREFERLSTVVLNAYLGPVMKSYIERLSPRLEALGME
HHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCC
ATPHLTQSNGGVIGFSTAAAMPVRTILSGPSTGVVAAQAVGELAGFPDIITFDMGGTSTD
CCCCEECCCCCEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCC
VALLKGGVCKLTSEANVHGYPLKAPMLDIHTVGAGGGSIAYVDSGGLLKVGPRSCGAFPG
HHHHCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCEEEECCCCCCCCCC
PVCYDKGNEEPATTDANVVLQTLNPTHLLAGRMPVRQDLAKAAIGRLADKLGLGVMETAQ
CEECCCCCCCCCCCCCCEEEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
GILSVVTANMAKAIRVISVQRGHDPRDYTLMAFGGAGPLHAARLAKELDMRRVLVPRNPG
HHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHCHHEEECCCCCC
ILCAMGLLLTDLRADFAATKLLPLAASSLAPVSEAFATLSARAQAWFDHEEITAAARQMH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RTVDMRYAGQNYELSVALPEGAVTETTLEHLAEAFEEAHRQRYGFIAAGEAVQIVTLRVE
HHHHHEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCEEEEEEEEEH
AVGLVRKATLKAYPLEGPDASAAIIGKRPVWFPEAGDYVPTPIYDREALKAGNRFAGPAI
HHHHHHHHHHEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHH
VEQMDTTTVVPPGMTARVDAYHNLILEVAA
HHHCCCCEECCCCCCCHHHHHHHHHHEECC
>Mature Secondary Structure 
ARWRVGVDSGGTFTDICLFDEDEGRVEVWKVSSTPDDPSRGIAEGVEQGMRRVAPENGD
CEEEEECCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCC
KPASSVAYFGHGTTVATNALIQHRGVATGLVTTDGFRDLLEIGRQKRPDLYDLTADKPPT
CCCCCEEEECCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCEECCCCCCCC
LVPRALRMAVGERVRHDGSIDLPLDEPAVREAAKALKKAGVKAIAVSFLYGFVRPEHEAR
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHH
AVEIIREEYPEAFVCSGHEIAPEFREFERLSTVVLNAYLGPVMKSYIERLSPRLEALGME
HHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCC
ATPHLTQSNGGVIGFSTAAAMPVRTILSGPSTGVVAAQAVGELAGFPDIITFDMGGTSTD
CCCCEECCCCCEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCC
VALLKGGVCKLTSEANVHGYPLKAPMLDIHTVGAGGGSIAYVDSGGLLKVGPRSCGAFPG
HHHHCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCEEEECCCCCCCCCC
PVCYDKGNEEPATTDANVVLQTLNPTHLLAGRMPVRQDLAKAAIGRLADKLGLGVMETAQ
CEECCCCCCCCCCCCCCEEEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
GILSVVTANMAKAIRVISVQRGHDPRDYTLMAFGGAGPLHAARLAKELDMRRVLVPRNPG
HHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHCHHEEECCCCCC
ILCAMGLLLTDLRADFAATKLLPLAASSLAPVSEAFATLSARAQAWFDHEEITAAARQMH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RTVDMRYAGQNYELSVALPEGAVTETTLEHLAEAFEEAHRQRYGFIAAGEAVQIVTLRVE
HHHHHEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCEEEEEEEEEH
AVGLVRKATLKAYPLEGPDASAAIIGKRPVWFPEAGDYVPTPIYDREALKAGNRFAGPAI
HHHHHHHHHHEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHH
VEQMDTTTVVPPGMTARVDAYHNLILEVAA
HHHCCCCEECCCCCCCHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]