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The map label for this gene is livJ [C]

Identifier: 158424571

GI number: 158424571

Start: 3368578

End: 3369789

Strand: Direct

Name: livJ [C]

Synonym: AZC_2947

Alternate gene names: 158424571

Gene position: 3368578-3369789 (Clockwise)

Preceding gene: 158424570

Following gene: 158424572

Centisome position: 62.73

GC content: 63.78

Gene sequence:

>1212_bases
ATGGCGGGCATCCACGTCCCTGAATCGACTGTACCAGTCACACATCTGCCCGTTGCAGCCGGCCTCGCGGGTTCCGTCTC
GCGACCGCTGGAAAAACCAGAGGAGATACGGATGAGGAAGCTTCTACTTGCCGGTCTTGCGCTTGGCGCCGGCCTGGCCT
TCGCGGCCCAGGCTCAGGCCCAGGTGAAGCTGGGCGTGGCCGGTCCCATGACCGGCCCGAATGCCGCTTTCGGCGCCCAG
CTCAAGAACGGCGTCGAGCAGGCCGTCACGGACATCAATGCGTCCGGCGGCATCCTCGGCCAGAAGATCCAGGTGTTCGT
GGGCGACGACGCCTCCACCCCGGCGCAGGGAACCTCCGTCGCCAACAAATTCATCTCCGACGGCGTGAAATACGTCGTGG
GCCACTTCAACTCGGGCGTCTCGATCCCGACCTCCACCAACTATGAGGAAGGCGGCATCCTCCAGATCACGCCCGCCTCC
ACCAACCCGACCTTCACCGAGCGCAAGCTCTGGAACGTGTTCCGCACCTGCGGCCGCGACGACCAGCAGGGCGCGGTGGC
CGGCGAGTACATCCTGAAGAACCTGAAGGACAAGAAGATCGCCATCGTCCACGACAAGACCCCCTATGGTAAGGGCCTAG
CGGACGAGACGCAGAAGGCCATCAACAAGGGTGGCGTGAAGGAAGTTGTCTATGAGGGCATCACGCCCGGCGAGAAGGAC
TATTCCGCTCTCGTGTCCAAGCTGAAGGCGGCCGGCGTCGAGGTGCTTTATTACGGCGGCCTGCACCCGGAAGCCGGCCT
GCTGGTGCGTCAGATGCGCGATCAGGGCATGAAGACCGTCCTGTTCTCCGGCGACGGCATCACCGACAAGGAATACTGGA
CCATCGCCGGCCCCGGCGCCGAAGGCACGCTGATGACCTTCGGCCCCGATCCGCGCAAGAACGCCGCCGCCAAGGAAGTG
GTGGACCGCTTCAAGGCCAAGGGCATCGATCCGGAAGGCTACGTGCTCTATTCCTACGCCGCCGTGCAGATCATCAAGCA
GGCGGCCGAGGCCACGAAATCCCTCGACCCGAAGAAGGTCGCCGACTACATGCACTCCGGCGCCACCTTCAACACCGTGC
TCGGCCCGCTCGCCTTCGACAAGAAGGGCGACCTGACCAAGCTCGACTATGTGGTCTACGTCTGGAAGGACGGCGGCTAT
TCGGAACTCTGA

Upstream 100 bases:

>100_bases
CGAGAGCTCCGGCCCCCTCGCCTGGCGTCCGCGCCAGGGCTGAACGCCACGCTGCCGCAGCCCCGCCTCCCCCATCGACA
GGGGACGGATTTGCGGCAGA

Downstream 100 bases:

>100_bases
CCCGGCCTTGCCGATCATATCCATCTGACAGGGAGACGCCCGCGCCGTGCGCGGGCGTTTTCTTTTGCGCCGGAGCGGTT
CGAATTCCGCGACGGCAGGC

Product: amino acid ABC transporter substrate-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MAGIHVPESTVPVTHLPVAAGLAGSVSRPLEKPEEIRMRKLLLAGLALGAGLAFAAQAQAQVKLGVAGPMTGPNAAFGAQ
LKNGVEQAVTDINASGGILGQKIQVFVGDDASTPAQGTSVANKFISDGVKYVVGHFNSGVSIPTSTNYEEGGILQITPAS
TNPTFTERKLWNVFRTCGRDDQQGAVAGEYILKNLKDKKIAIVHDKTPYGKGLADETQKAINKGGVKEVVYEGITPGEKD
YSALVSKLKAAGVEVLYYGGLHPEAGLLVRQMRDQGMKTVLFSGDGITDKEYWTIAGPGAEGTLMTFGPDPRKNAAAKEV
VDRFKAKGIDPEGYVLYSYAAVQIIKQAAEATKSLDPKKVADYMHSGATFNTVLGPLAFDKKGDLTKLDYVVYVWKDGGY
SEL

Sequences:

>Translated_403_residues
MAGIHVPESTVPVTHLPVAAGLAGSVSRPLEKPEEIRMRKLLLAGLALGAGLAFAAQAQAQVKLGVAGPMTGPNAAFGAQ
LKNGVEQAVTDINASGGILGQKIQVFVGDDASTPAQGTSVANKFISDGVKYVVGHFNSGVSIPTSTNYEEGGILQITPAS
TNPTFTERKLWNVFRTCGRDDQQGAVAGEYILKNLKDKKIAIVHDKTPYGKGLADETQKAINKGGVKEVVYEGITPGEKD
YSALVSKLKAAGVEVLYYGGLHPEAGLLVRQMRDQGMKTVLFSGDGITDKEYWTIAGPGAEGTLMTFGPDPRKNAAAKEV
VDRFKAKGIDPEGYVLYSYAAVQIIKQAAEATKSLDPKKVADYMHSGATFNTVLGPLAFDKKGDLTKLDYVVYVWKDGGY
SEL
>Mature_402_residues
AGIHVPESTVPVTHLPVAAGLAGSVSRPLEKPEEIRMRKLLLAGLALGAGLAFAAQAQAQVKLGVAGPMTGPNAAFGAQL
KNGVEQAVTDINASGGILGQKIQVFVGDDASTPAQGTSVANKFISDGVKYVVGHFNSGVSIPTSTNYEEGGILQITPAST
NPTFTERKLWNVFRTCGRDDQQGAVAGEYILKNLKDKKIAIVHDKTPYGKGLADETQKAINKGGVKEVVYEGITPGEKDY
SALVSKLKAAGVEVLYYGGLHPEAGLLVRQMRDQGMKTVLFSGDGITDKEYWTIAGPGAEGTLMTFGPDPRKNAAAKEVV
DRFKAKGIDPEGYVLYSYAAVQIIKQAAEATKSLDPKKVADYMHSGATFNTVLGPLAFDKKGDLTKLDYVVYVWKDGGYS
EL

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=362, Percent_Identity=44.7513812154696, Blast_Score=289, Evalue=3e-79,
Organism=Escherichia coli, GI1789867, Length=368, Percent_Identity=42.9347826086957, Blast_Score=278, Evalue=4e-76,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 42695; Mature: 42564

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGIHVPESTVPVTHLPVAAGLAGSVSRPLEKPEEIRMRKLLLAGLALGAGLAFAAQAQA
CCCCCCCCCCCCEEECCHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCE
QVKLGVAGPMTGPNAAFGAQLKNGVEQAVTDINASGGILGQKIQVFVGDDASTPAQGTSV
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHH
ANKFISDGVKYVVGHFNSGVSIPTSTNYEEGGILQITPASTNPTFTERKLWNVFRTCGRD
HHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCC
DQQGAVAGEYILKNLKDKKIAIVHDKTPYGKGLADETQKAINKGGVKEVVYEGITPGEKD
CCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCHH
YSALVSKLKAAGVEVLYYGGLHPEAGLLVRQMRDQGMKTVLFSGDGITDKEYWTIAGPGA
HHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCC
EGTLMTFGPDPRKNAAAKEVVDRFKAKGIDPEGYVLYSYAAVQIIKQAAEATKSLDPKKV
CCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCHHHH
ADYMHSGATFNTVLGPLAFDKKGDLTKLDYVVYVWKDGGYSEL
HHHHHCCCCHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCC
>Mature Secondary Structure 
AGIHVPESTVPVTHLPVAAGLAGSVSRPLEKPEEIRMRKLLLAGLALGAGLAFAAQAQA
CCCCCCCCCCCEEECCHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCE
QVKLGVAGPMTGPNAAFGAQLKNGVEQAVTDINASGGILGQKIQVFVGDDASTPAQGTSV
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHH
ANKFISDGVKYVVGHFNSGVSIPTSTNYEEGGILQITPASTNPTFTERKLWNVFRTCGRD
HHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCC
DQQGAVAGEYILKNLKDKKIAIVHDKTPYGKGLADETQKAINKGGVKEVVYEGITPGEKD
CCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCHH
YSALVSKLKAAGVEVLYYGGLHPEAGLLVRQMRDQGMKTVLFSGDGITDKEYWTIAGPGA
HHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCC
EGTLMTFGPDPRKNAAAKEVVDRFKAKGIDPEGYVLYSYAAVQIIKQAAEATKSLDPKKV
CCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCHHHH
ADYMHSGATFNTVLGPLAFDKKGDLTKLDYVVYVWKDGGYSEL
HHHHHCCCCHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA