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The map label for this gene is proP [H]

Identifier: 158424503

GI number: 158424503

Start: 3280349

End: 3281692

Strand: Direct

Name: proP [H]

Synonym: AZC_2879

Alternate gene names: 158424503

Gene position: 3280349-3281692 (Clockwise)

Preceding gene: 158424502

Following gene: 158424504

Centisome position: 61.09

GC content: 67.56

Gene sequence:

>1344_bases
ATGACCAGCATTTCGCAGACCAGCGCACGGCATGCACCGGCGCATCTCAGCCGGGCACAGTGGCGCATCATCCTGCTCGC
CAGCCTCGGTGGATCGCTCGAATTCTACGATTTCATCATCTACGGCATCTTCGTCCAGTACATCTCGGCGCAGTTCTTTC
CCGCCAACGATCCCTATGTCTCGCTGATCCTGTCCTTCTCCGTGCTCGCGCTGGGCTTTCTCGCCCGCCCCTTCGGCGGC
GCACTGCTGGGCACGCTGGGCGATCGCTACGGCCGGCGGCCGGTGTTCATCGCCTCGCTCGGCCTCACCACGCTGGCCAC
CATCGCCATCGGTCTGCTGCCGGGCTACGACCGCTGGGGCATCGCCGCCCCGCTGCTGCTGGTCGCCTTCCGTCTGGTGC
AGGGCGTCTGCCTCGGCGGCGAACTGCCGGGTGCCGTCACCTACGCCGTGGAAGCCGCGCCGCGCCGGGCGGGCCTTGCC
TGCGGCTTCATCATCTTCTGCGTCAACGTGGGCGTGCTGATCGCCACCCTCGTCAATCTGGGCATCCAGTATGCGCTGCC
GCCGCAGGATGTCGCCTCCTATGGCTGGCGCATCGCCTTCCTTTTCGGCGGCGTCATCGGCCTCGTCAGCTTCGCCCTGC
GCCGCAAGCTCGAAGAATCGCCGGAATTCGTGAACCTGCACGGCAAGGCGGCCAAGCATCCGCTGGGCGAACTGGTGCGC
AACCACGGCCGCGCGCTGCTGGTGGCGGCCGGTGTCGCGGCGATCATCGCCGGCTTCAACGGCATGCTCTACGGCTTCGT
ACCGGCCTTCCTGGTGAAGACCCTCGGCTATCCCGCGCCTGAGGTGGCCCTTGCCATGACCGTGGCGCTGGTGGTGAGTT
CCATCGGCCTGATGATCGCGGGATGGGCGGGCGACTTCACGTCGGCGCGCCTCATCCTGCGGGCCGGCTCGCTGCTGATG
CTGATCACGGTGGTGCCGCTGTTCGCGCTGATGGCCAACCGGGGAGCCAGCATCGTGCTCGTGCTCGCGCTGCTCTCGCT
GGTCTTCTCGCTTGCCAGCGGCATCTGGCCGAGCATCCTCGCCACCCTGTTCCCGACCAAGGTGCGCTTCACCGGCATCG
CTCTATCCTACAACATCAGCGTCACCATTCTCTCCGGCTTCGCGCCGCTCGCCGCCACCATGCTGATCGAGCGCACGCAG
ATGCCCTCCGCGCCCGCCCTTTACATCGCTCTGTGCGCGGCCCTCAGCCTGTGGAGCAGCTTCCTCGTGCCGGAGCGCCG
GGGGGCGTCACGGGATGAGCGGGTGGAAGCCCCGCTCAAGGCCTCGGGCCTGCCTCCGCTCTGA

Upstream 100 bases:

>100_bases
ATGCTCCCGTCTCTGCTTCCGGTGGCATGGGCAAACCTGCCCGCCGACCGGACAAGCGTATTCCGCCCCAACAAAAAGAT
CAGCCGGAGGGCTCAAGGAC

Downstream 100 bases:

>100_bases
GCCGTGTCCGCCCAGCGGACCCCAAGCGACCGGCGGGCGACAGGCCCGCCGGTTCCGCTTAATCAGGACGCGCGGTCTGG
ACGCCGCATCCCGAGCCTTT

Product: major facilitator superfamily permease

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MTSISQTSARHAPAHLSRAQWRIILLASLGGSLEFYDFIIYGIFVQYISAQFFPANDPYVSLILSFSVLALGFLARPFGG
ALLGTLGDRYGRRPVFIASLGLTTLATIAIGLLPGYDRWGIAAPLLLVAFRLVQGVCLGGELPGAVTYAVEAAPRRAGLA
CGFIIFCVNVGVLIATLVNLGIQYALPPQDVASYGWRIAFLFGGVIGLVSFALRRKLEESPEFVNLHGKAAKHPLGELVR
NHGRALLVAAGVAAIIAGFNGMLYGFVPAFLVKTLGYPAPEVALAMTVALVVSSIGLMIAGWAGDFTSARLILRAGSLLM
LITVVPLFALMANRGASIVLVLALLSLVFSLASGIWPSILATLFPTKVRFTGIALSYNISVTILSGFAPLAATMLIERTQ
MPSAPALYIALCAALSLWSSFLVPERRGASRDERVEAPLKASGLPPL

Sequences:

>Translated_447_residues
MTSISQTSARHAPAHLSRAQWRIILLASLGGSLEFYDFIIYGIFVQYISAQFFPANDPYVSLILSFSVLALGFLARPFGG
ALLGTLGDRYGRRPVFIASLGLTTLATIAIGLLPGYDRWGIAAPLLLVAFRLVQGVCLGGELPGAVTYAVEAAPRRAGLA
CGFIIFCVNVGVLIATLVNLGIQYALPPQDVASYGWRIAFLFGGVIGLVSFALRRKLEESPEFVNLHGKAAKHPLGELVR
NHGRALLVAAGVAAIIAGFNGMLYGFVPAFLVKTLGYPAPEVALAMTVALVVSSIGLMIAGWAGDFTSARLILRAGSLLM
LITVVPLFALMANRGASIVLVLALLSLVFSLASGIWPSILATLFPTKVRFTGIALSYNISVTILSGFAPLAATMLIERTQ
MPSAPALYIALCAALSLWSSFLVPERRGASRDERVEAPLKASGLPPL
>Mature_446_residues
TSISQTSARHAPAHLSRAQWRIILLASLGGSLEFYDFIIYGIFVQYISAQFFPANDPYVSLILSFSVLALGFLARPFGGA
LLGTLGDRYGRRPVFIASLGLTTLATIAIGLLPGYDRWGIAAPLLLVAFRLVQGVCLGGELPGAVTYAVEAAPRRAGLAC
GFIIFCVNVGVLIATLVNLGIQYALPPQDVASYGWRIAFLFGGVIGLVSFALRRKLEESPEFVNLHGKAAKHPLGELVRN
HGRALLVAAGVAAIIAGFNGMLYGFVPAFLVKTLGYPAPEVALAMTVALVVSSIGLMIAGWAGDFTSARLILRAGSLLML
ITVVPLFALMANRGASIVLVLALLSLVFSLASGIWPSILATLFPTKVRFTGIALSYNISVTILSGFAPLAATMLIERTQM
PSAPALYIALCAALSLWSSFLVPERRGASRDERVEAPLKASGLPPL

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=435, Percent_Identity=28.0459770114943, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1789941, Length=444, Percent_Identity=33.3333333333333, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1788292, Length=416, Percent_Identity=28.6057692307692, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1788942, Length=425, Percent_Identity=30.5882352941176, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI87082231, Length=500, Percent_Identity=25.6, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI87082404, Length=369, Percent_Identity=27.10027100271, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47315; Mature: 47184

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSISQTSARHAPAHLSRAQWRIILLASLGGSLEFYDFIIYGIFVQYISAQFFPANDPYV
CCCCCCCCCCCCHHHHHHHCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHH
SLILSFSVLALGFLARPFGGALLGTLGDRYGRRPVFIASLGLTTLATIAIGLLPGYDRWG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCH
IAAPLLLVAFRLVQGVCLGGELPGAVTYAVEAAPRRAGLACGFIIFCVNVGVLIATLVNL
HHHHHHHHHHHHHHCEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHC
GIQYALPPQDVASYGWRIAFLFGGVIGLVSFALRRKLEESPEFVNLHGKAAKHPLGELVR
CCEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHCCHHHHHHH
NHGRALLVAAGVAAIIAGFNGMLYGFVPAFLVKTLGYPAPEVALAMTVALVVSSIGLMIA
CCCCEEEHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEE
GWAGDFTSARLILRAGSLLMLITVVPLFALMANRGASIVLVLALLSLVFSLASGIWPSIL
ECCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHHHH
ATLFPTKVRFTGIALSYNISVTILSGFAPLAATMLIERTQMPSAPALYIALCAALSLWSS
HHHCCCCEEEEEEEEEEEEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
FLVPERRGASRDERVEAPLKASGLPPL
HCCCCCCCCCCHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TSISQTSARHAPAHLSRAQWRIILLASLGGSLEFYDFIIYGIFVQYISAQFFPANDPYV
CCCCCCCCCCCHHHHHHHCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHH
SLILSFSVLALGFLARPFGGALLGTLGDRYGRRPVFIASLGLTTLATIAIGLLPGYDRWG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCH
IAAPLLLVAFRLVQGVCLGGELPGAVTYAVEAAPRRAGLACGFIIFCVNVGVLIATLVNL
HHHHHHHHHHHHHHCEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHC
GIQYALPPQDVASYGWRIAFLFGGVIGLVSFALRRKLEESPEFVNLHGKAAKHPLGELVR
CCEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHCCHHHHHHH
NHGRALLVAAGVAAIIAGFNGMLYGFVPAFLVKTLGYPAPEVALAMTVALVVSSIGLMIA
CCCCEEEHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEE
GWAGDFTSARLILRAGSLLMLITVVPLFALMANRGASIVLVLALLSLVFSLASGIWPSIL
ECCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHHHH
ATLFPTKVRFTGIALSYNISVTILSGFAPLAATMLIERTQMPSAPALYIALCAALSLWSS
HHHCCCCEEEEEEEEEEEEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
FLVPERRGASRDERVEAPLKASGLPPL
HCCCCCCCCCCHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]