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The map label for this gene is pyrB

Identifier: 158424475

GI number: 158424475

Start: 3245287

End: 3246252

Strand: Reverse

Name: pyrB

Synonym: AZC_2851

Alternate gene names: 158424475

Gene position: 3246252-3245287 (Counterclockwise)

Preceding gene: 158424477

Following gene: 158424474

Centisome position: 60.45

GC content: 66.25

Gene sequence:

>966_bases
ATGATCACAGCCCCATTCTTCACCCTCGATCGCCGCGACCTCCTCGGCATCGAAGGCCTTTCCGCGCCGGAAATCGTCGG
CCTCCTAGACCTGGCTGAGGAATTCGTCGTCCTCAACCGGCAGATCGAGAAAAAGCGTTCGACCTTGCGGGGCCGCACGC
AGGTGAACCTGTTCTTCGAGGCCTCCACCCGCACCCAGTCCTCCTTCGAGCTGGCGGGCAAGCGCCTCGGGGCCGACGTG
ATGAACATGTCGGTGGGCAATTCCTCGGTGAAGAAGGGCGAGACGCTCATCGACACCGCCATGACGCTGAACGCCATGCG
CCCCGACATCCTGGTGGTGCGCCATCATGCGTCGGGCGCCGTGGCGCTGCTCGCCCGCAAGGTGGACTGCTGCGTGGTGA
ATGCCGGCGACGGCGCCCATGAGCATCCCACCCAGGCGCTGCTCGACGCCCTCACCATCCGCCGCAACAAGGGGCGGATC
GAGGGGCTGACGGTGGCGGTGTGCGGCGATGTCCTGCATTCGCGCGTCGCCCGCTCCAACATCCTGCTCCTGCACACGCT
CGGCGCGCGGGTGCGGGTGGTGGCGCCGTCTACGCTGCTGCCCACCGGCATCGAGAATTTCGGCGTCGAGGTGCACCGCT
CCATGGAGAGCGGTCTCGAAGGGGCGGACATCGTGATGATGTTGCGGCTCCAGCGCGAGCGCATGGCCGGCTCCTTCATC
CCCTCGGTGAAGGAATATTTCCATTATTACGGCCTCGACGAAGCCAAGCTGAAACGGGCCAAGCCCGATGCGCTGGTGAT
GCATCCCGGCCCCATGAACCGGGGCGTCGAGATCGATTCGGCGGTGGCGGACGGTGCGCAGTCGCTGATCCGCGAGCAGG
TGGAAATGGGCGTCGCCGTGCGCATGGCGGTGCTGGACGCTCTGGCGCGCAAGCTGCCCAACGCGACGGGAGCCGAACGT
GACTGA

Upstream 100 bases:

>100_bases
AAATCGCAAGATTTGCGGCGGAAGCGTGGCGACGGTCCGGGCGCGCGGCGCAGGGCGAGCTTGCTCACCCGCGTGAGTCT
CGCTAAGCCTGCGGCCTGCC

Downstream 100 bases:

>100_bases
GCCGCATCCGCTTCTTCTCGCCAACGCCCGCGTCATCGATCCCTCGCGCGGCGCCGATTTCCGCGGCGACGTGTTGCTCG
CCAGCGGCGTCATCAAGGAC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MITAPFFTLDRRDLLGIEGLSAPEIVGLLDLAEEFVVLNRQIEKKRSTLRGRTQVNLFFEASTRTQSSFELAGKRLGADV
MNMSVGNSSVKKGETLIDTAMTLNAMRPDILVVRHHASGAVALLARKVDCCVVNAGDGAHEHPTQALLDALTIRRNKGRI
EGLTVAVCGDVLHSRVARSNILLLHTLGARVRVVAPSTLLPTGIENFGVEVHRSMESGLEGADIVMMLRLQRERMAGSFI
PSVKEYFHYYGLDEAKLKRAKPDALVMHPGPMNRGVEIDSAVADGAQSLIREQVEMGVAVRMAVLDALARKLPNATGAER
D

Sequences:

>Translated_321_residues
MITAPFFTLDRRDLLGIEGLSAPEIVGLLDLAEEFVVLNRQIEKKRSTLRGRTQVNLFFEASTRTQSSFELAGKRLGADV
MNMSVGNSSVKKGETLIDTAMTLNAMRPDILVVRHHASGAVALLARKVDCCVVNAGDGAHEHPTQALLDALTIRRNKGRI
EGLTVAVCGDVLHSRVARSNILLLHTLGARVRVVAPSTLLPTGIENFGVEVHRSMESGLEGADIVMMLRLQRERMAGSFI
PSVKEYFHYYGLDEAKLKRAKPDALVMHPGPMNRGVEIDSAVADGAQSLIREQVEMGVAVRMAVLDALARKLPNATGAER
D
>Mature_321_residues
MITAPFFTLDRRDLLGIEGLSAPEIVGLLDLAEEFVVLNRQIEKKRSTLRGRTQVNLFFEASTRTQSSFELAGKRLGADV
MNMSVGNSSVKKGETLIDTAMTLNAMRPDILVVRHHASGAVALLARKVDCCVVNAGDGAHEHPTQALLDALTIRRNKGRI
EGLTVAVCGDVLHSRVARSNILLLHTLGARVRVVAPSTLLPTGIENFGVEVHRSMESGLEGADIVMMLRLQRERMAGSFI
PSVKEYFHYYGLDEAKLKRAKPDALVMHPGPMNRGVEIDSAVADGAQSLIREQVEMGVAVRMAVLDALARKLPNATGAER
D

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=316, Percent_Identity=36.0759493670886, Blast_Score=169, Evalue=3e-42,
Organism=Escherichia coli, GI2367364, Length=269, Percent_Identity=37.1747211895911, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI193204318, Length=329, Percent_Identity=34.6504559270517, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6322331, Length=268, Percent_Identity=36.9402985074627, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24642586, Length=267, Percent_Identity=37.4531835205993, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_AZOC5 (A8ICW0)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001525767.1
- ProteinModelPortal:   A8ICW0
- SMR:   A8ICW0
- GeneID:   5689256
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_2851
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   ACAU438753:AZC_2851-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34924; Mature: 34924

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITAPFFTLDRRDLLGIEGLSAPEIVGLLDLAEEFVVLNRQIEKKRSTLRGRTQVNLFFE
CCCCCEEECCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
ASTRTQSSFELAGKRLGADVMNMSVGNSSVKKGETLIDTAMTLNAMRPDILVVRHHASGA
ECCCCCHHHHHHHHHHCCHHHHCCCCCCCHHCCCHHHHHHHHHHCCCCCEEEEEECCCCH
VALLARKVDCCVVNAGDGAHEHPTQALLDALTIRRNKGRIEGLTVAVCGDVLHSRVARSN
HHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCC
ILLLHTLGARVRVVAPSTLLPTGIENFGVEVHRSMESGLEGADIVMMLRLQRERMAGSFI
EEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
PSVKEYFHYYGLDEAKLKRAKPDALVMHPGPMNRGVEIDSAVADGAQSLIREQVEMGVAV
HHHHHHHHHCCCCHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RMAVLDALARKLPNATGAERD
HHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MITAPFFTLDRRDLLGIEGLSAPEIVGLLDLAEEFVVLNRQIEKKRSTLRGRTQVNLFFE
CCCCCEEECCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
ASTRTQSSFELAGKRLGADVMNMSVGNSSVKKGETLIDTAMTLNAMRPDILVVRHHASGA
ECCCCCHHHHHHHHHHCCHHHHCCCCCCCHHCCCHHHHHHHHHHCCCCCEEEEEECCCCH
VALLARKVDCCVVNAGDGAHEHPTQALLDALTIRRNKGRIEGLTVAVCGDVLHSRVARSN
HHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCC
ILLLHTLGARVRVVAPSTLLPTGIENFGVEVHRSMESGLEGADIVMMLRLQRERMAGSFI
EEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
PSVKEYFHYYGLDEAKLKRAKPDALVMHPGPMNRGVEIDSAVADGAQSLIREQVEMGVAV
HHHHHHHHHCCCCHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RMAVLDALARKLPNATGAERD
HHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA