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The map label for this gene is hisJ [H]

Identifier: 158424359

GI number: 158424359

Start: 3104666

End: 3105499

Strand: Direct

Name: hisJ [H]

Synonym: AZC_2735

Alternate gene names: 158424359

Gene position: 3104666-3105499 (Clockwise)

Preceding gene: 158424358

Following gene: 158424360

Centisome position: 57.82

GC content: 67.27

Gene sequence:

>834_bases
TTGGAGACGCACATGCTGAAACGCCTTCTGCTCAGCGCCTGCCTGCTTCTTCCCTTCGCCGCCGGCGCGGCGGAGATCCC
GGCCGAGATCAAGGCGCGCGGCACGATCACGGTCGCCGTCATTCCCAATTATCCGCCGCTGGAGTTCAAGGACCCCGCCA
CCGGCCAGCTCTCCGGCTTCGACATCGCGCTCGGCAATGCGCTGGCCGAAAAGCTGGGGCTCAAGATGCAGTGGCAGGAG
ACGAGCTTCGAGGCCATGCTGCCGGCGCTGAAGACCGGGCGCGTGGACATCATTCTCTCCGGCATGAGCGACCTGCCCAC
CCGCCGCGATGGCGCGAGCTTCATCGACTATCTGCGCTCGGGCACGCAATTCTTCACGCAGGCGAGCCGCGCGTCGGAAT
TCCCGAACCGCGAGGCCCTGTGCGGCCAGAAGGTGGGCGCCAGCCGCCGCACTTCGCTCCCCGGCGTCATCAAGGACTTC
TCGGACACCGCCTGCGTCGGCACCGGCAAGCCGGCGATCGTTGTGGTGGGCACCGAAGGCTCGGCGGATGCGCGCACGCA
GCTCCGACAGGGCCGCATCGATGCCGCCATGCAGGGCGACGAGACGCTGCCCTACATCATGGCCCAGGAGCCCAACACCT
ACGCTCTGGTGGGTGAGCCCACGCGCTGGACGCTCATGGGCCTTGCCACCAACAAGGAGGCCACCGGCCTGCGCGATGCC
CTCGCGGACGCCCTGCGCGGGCTGATCGCCGATGGCACATACGCCAAGCTGCTGGCCGCCTGGACGCTTTCCCGAAACGG
CATTCAAGAGGTCACCATAGATGCCGGACAATGA

Upstream 100 bases:

>100_bases
GCCGCATCATCGAGGAGGGCAGCGCCGAACAGGTGCTCGGCCAGCCCCGCGAGGCCCGCACGCAGGCTTTTCTCTCCGCC
GTTCTTGGCTAATCCCTTCT

Downstream 100 bases:

>100_bases
CGCCTTCGCGCGCCTTCCGCTGGCCCCGGCCAACCGCGCGCCGGCCGCCCCCCTCATCCCCTGGCCGCACGGCAAGCGCG
GCGCCATGTTCCTCTCCGCC

Product: ABC transporter substrate-binding protein

Products: ADP; phosphate; L-histidine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
METHMLKRLLLSACLLLPFAAGAAEIPAEIKARGTITVAVIPNYPPLEFKDPATGQLSGFDIALGNALAEKLGLKMQWQE
TSFEAMLPALKTGRVDIILSGMSDLPTRRDGASFIDYLRSGTQFFTQASRASEFPNREALCGQKVGASRRTSLPGVIKDF
SDTACVGTGKPAIVVVGTEGSADARTQLRQGRIDAAMQGDETLPYIMAQEPNTYALVGEPTRWTLMGLATNKEATGLRDA
LADALRGLIADGTYAKLLAAWTLSRNGIQEVTIDAGQ

Sequences:

>Translated_277_residues
METHMLKRLLLSACLLLPFAAGAAEIPAEIKARGTITVAVIPNYPPLEFKDPATGQLSGFDIALGNALAEKLGLKMQWQE
TSFEAMLPALKTGRVDIILSGMSDLPTRRDGASFIDYLRSGTQFFTQASRASEFPNREALCGQKVGASRRTSLPGVIKDF
SDTACVGTGKPAIVVVGTEGSADARTQLRQGRIDAAMQGDETLPYIMAQEPNTYALVGEPTRWTLMGLATNKEATGLRDA
LADALRGLIADGTYAKLLAAWTLSRNGIQEVTIDAGQ
>Mature_277_residues
METHMLKRLLLSACLLLPFAAGAAEIPAEIKARGTITVAVIPNYPPLEFKDPATGQLSGFDIALGNALAEKLGLKMQWQE
TSFEAMLPALKTGRVDIILSGMSDLPTRRDGASFIDYLRSGTQFFTQASRASEFPNREALCGQKVGASRRTSLPGVIKDF
SDTACVGTGKPAIVVVGTEGSADARTQLRQGRIDAAMQGDETLPYIMAQEPNTYALVGEPTRWTLMGLATNKEATGLRDA
LADALRGLIADGTYAKLLAAWTLSRNGIQEVTIDAGQ

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788648, Length=234, Percent_Identity=29.0598290598291, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1787088, Length=262, Percent_Identity=26.7175572519084, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787085, Length=262, Percent_Identity=26.3358778625954, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788649, Length=260, Percent_Identity=26.1538461538462, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788228, Length=234, Percent_Identity=25.6410256410256, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787031, Length=254, Percent_Identity=25.9842519685039, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 3400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29590; Mature: 29590

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METHMLKRLLLSACLLLPFAAGAAEIPAEIKARGTITVAVIPNYPPLEFKDPATGQLSGF
CCHHHHHHHHHHHHHHHHHCCCCHHCCHHEECCCEEEEEECCCCCCCCCCCCCCCCCCCC
DIALGNALAEKLGLKMQWQETSFEAMLPALKTGRVDIILSGMSDLPTRRDGASFIDYLRS
EEHHHHHHHHHHCCEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHC
GTQFFTQASRASEFPNREALCGQKVGASRRTSLPGVIKDFSDTACVGTGKPAIVVVGTEG
CHHHHHHHHHHHCCCCCHHHCCHHCCCCCCCCCCHHHHCCCCCEEECCCCCEEEEEECCC
SADARTQLRQGRIDAAMQGDETLPYIMAQEPNTYALVGEPTRWTLMGLATNKEATGLRDA
CCHHHHHHHHCCCCHHCCCCCCCCEEEECCCCEEEEEECCCEEEEEEEECCCCCCHHHHH
LADALRGLIADGTYAKLLAAWTLSRNGIQEVTIDAGQ
HHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCC
>Mature Secondary Structure
METHMLKRLLLSACLLLPFAAGAAEIPAEIKARGTITVAVIPNYPPLEFKDPATGQLSGF
CCHHHHHHHHHHHHHHHHHCCCCHHCCHHEECCCEEEEEECCCCCCCCCCCCCCCCCCCC
DIALGNALAEKLGLKMQWQETSFEAMLPALKTGRVDIILSGMSDLPTRRDGASFIDYLRS
EEHHHHHHHHHHCCEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHC
GTQFFTQASRASEFPNREALCGQKVGASRRTSLPGVIKDFSDTACVGTGKPAIVVVGTEG
CHHHHHHHHHHHCCCCCHHHCCHHCCCCCCCCCCHHHHCCCCCEEECCCCCEEEEEECCC
SADARTQLRQGRIDAAMQGDETLPYIMAQEPNTYALVGEPTRWTLMGLATNKEATGLRDA
CCHHHHHHHHCCCCHHCCCCCCCCEEEECCCCEEEEEECCCEEEEEEEECCCCCCHHHHH
LADALRGLIADGTYAKLLAAWTLSRNGIQEVTIDAGQ
HHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]