Click here to switch to the map view.

The map label for this gene is potD [C]

Identifier: 158424348

GI number: 158424348

Start: 3093404

End: 3094465

Strand: Direct

Name: potD [C]

Synonym: AZC_2724

Alternate gene names: 158424348

Gene position: 3093404-3094465 (Clockwise)

Preceding gene: 158424347

Following gene: 158424349

Centisome position: 57.61

GC content: 64.41

Gene sequence:

>1062_bases
ATGACCGAGCACAGGACGTCCCTCAGCCGCCGTCAGTTCCTCGCCGCCGGCGCCGCTCTCGGGGCGGGCGCCATCGCCGC
CCCGCATGTCTCGCGCGCCCAGGAGGCGGCGACGCTGCGCATCACGGGCTGGGGCGGCAAGTGGGGGCAGGTCATGTCGG
CGGACGTGGTTCCCGCCTTCGAAAAGGAGTTCAAGTGCAAGGTGGTCACGGACACCGCTTTGCCGTTCCTGCCGAAGCTG
CAGGCGAGCCCGCGCTCGGCGCCCATCTATGACGTGCTGCACACCAATTCCAACGAACAGTGGGCGGCGGTGGAGATGGG
GCTCGTCGAGCCCAAGATCGACCCCAAGCTCGTGCCCAACCTTGCCGACGTCTATCCGTTTGCCATCAGCGACAAGATCG
TTGGCGTCTCCATCTTCACCAGCGCCATCGGCCTCGGCATGCGCACGGACAAGGGCTATCCGACGCCGACCTCCTGGAAG
GACCTCTGGGACGAGCGCTACAACGGTGTGCGTGGCGGCTATGTGATCCCGGTGAACAGCCTCGGCCAGGCCTTCGTGCC
CATGTGCGGCATGCTCTACGGCAAGGGACCGACCGATCTCGACGCCGCCTATGCGGCGCTGGAAAAGGTGAAGCCGATCA
AGCTCGTGGACTTCACCGGCGCGATGGAGAAGATGCTCCTCTCCGGCGAAGTCGGCATGTGCATCATCCACGACAGCGGC
ATCAACCGCTATTATGACGACAAGCCGCCGACCGCTTTCGTGGCGCCGAAGGAAGGTGTTCTGGCGCTGGAGCAGGTGCT
CACCATCACCCCCGGCAGCAAGGTGAAGGAACTCGCCAACGCTTACGTCAACTACATGCTCTCGCCGGACGTGCAGAAGA
AGCTGGCGCAGGCGGTGTGGTATTCGCCCTCCAACAAGATGGTGAAGCTGGACGAGCCCTATGCCTCCCGCCTGCTCACC
ACCCCGGAGAAGGTGGCGAGCCTCATCCAGGTGGACTGGAAGTGGTACAATGCCCACAAGGACGCCATCGACAGCCGCGT
GAACCGGATCTTCCGCGCATGA

Upstream 100 bases:

>100_bases
TCCTCGCCGCGCTGGTCGTCATCGGCCTCGACCGCACGGTGGGTCTCCGCTCCTACGTCAAGCTCTGAAAACAAACACCT
TCCAGACGGGAAACCCAGCC

Downstream 100 bases:

>100_bases
GCGCACGGATCGAAATCGCCGGCGTGTCCAAGAGCTATGACGGCGCCACCCGCGCCGTCGATGCCATCGACATGACCATC
GAGCCGGGGGAGTTCTTCTC

Product: ABC transporter substrate-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: ABC Transporter; Spermidine/Putrescine ABC Transporter; Transport System Periplasmic Protein

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MTEHRTSLSRRQFLAAGAALGAGAIAAPHVSRAQEAATLRITGWGGKWGQVMSADVVPAFEKEFKCKVVTDTALPFLPKL
QASPRSAPIYDVLHTNSNEQWAAVEMGLVEPKIDPKLVPNLADVYPFAISDKIVGVSIFTSAIGLGMRTDKGYPTPTSWK
DLWDERYNGVRGGYVIPVNSLGQAFVPMCGMLYGKGPTDLDAAYAALEKVKPIKLVDFTGAMEKMLLSGEVGMCIIHDSG
INRYYDDKPPTAFVAPKEGVLALEQVLTITPGSKVKELANAYVNYMLSPDVQKKLAQAVWYSPSNKMVKLDEPYASRLLT
TPEKVASLIQVDWKWYNAHKDAIDSRVNRIFRA

Sequences:

>Translated_353_residues
MTEHRTSLSRRQFLAAGAALGAGAIAAPHVSRAQEAATLRITGWGGKWGQVMSADVVPAFEKEFKCKVVTDTALPFLPKL
QASPRSAPIYDVLHTNSNEQWAAVEMGLVEPKIDPKLVPNLADVYPFAISDKIVGVSIFTSAIGLGMRTDKGYPTPTSWK
DLWDERYNGVRGGYVIPVNSLGQAFVPMCGMLYGKGPTDLDAAYAALEKVKPIKLVDFTGAMEKMLLSGEVGMCIIHDSG
INRYYDDKPPTAFVAPKEGVLALEQVLTITPGSKVKELANAYVNYMLSPDVQKKLAQAVWYSPSNKMVKLDEPYASRLLT
TPEKVASLIQVDWKWYNAHKDAIDSRVNRIFRA
>Mature_352_residues
TEHRTSLSRRQFLAAGAALGAGAIAAPHVSRAQEAATLRITGWGGKWGQVMSADVVPAFEKEFKCKVVTDTALPFLPKLQ
ASPRSAPIYDVLHTNSNEQWAAVEMGLVEPKIDPKLVPNLADVYPFAISDKIVGVSIFTSAIGLGMRTDKGYPTPTSWKD
LWDERYNGVRGGYVIPVNSLGQAFVPMCGMLYGKGPTDLDAAYAALEKVKPIKLVDFTGAMEKMLLSGEVGMCIIHDSGI
NRYYDDKPPTAFVAPKEGVLALEQVLTITPGSKVKELANAYVNYMLSPDVQKKLAQAVWYSPSNKMVKLDEPYASRLLTT
PEKVASLIQVDWKWYNAHKDAIDSRVNRIFRA

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 38695; Mature: 38564

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHRTSLSRRQFLAAGAALGAGAIAAPHVSRAQEAATLRITGWGGKWGQVMSADVVPAF
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCEEEEEEECCCCCCCCEECCCCCHH
EKEFKCKVVTDTALPFLPKLQASPRSAPIYDVLHTNSNEQWAAVEMGLVEPKIDPKLVPN
HHCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCHHCCC
LADVYPFAISDKIVGVSIFTSAIGLGMRTDKGYPTPTSWKDLWDERYNGVRGGYVIPVNS
HHHHCCHHHCCCEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEHHH
LGQAFVPMCGMLYGKGPTDLDAAYAALEKVKPIKLVDFTGAMEKMLLSGEVGMCIIHDSG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCEEEEEECC
INRYYDDKPPTAFVAPKEGVLALEQVLTITPGSKVKELANAYVNYMLSPDVQKKLAQAVW
CCCCCCCCCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHE
YSPSNKMVKLDEPYASRLLTTPEKVASLIQVDWKWYNAHKDAIDSRVNRIFRA
ECCCCCEEEECCHHHHHHCCCHHHHHHHHHHHHHEECHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TEHRTSLSRRQFLAAGAALGAGAIAAPHVSRAQEAATLRITGWGGKWGQVMSADVVPAF
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCEEEEEEECCCCCCCCEECCCCCHH
EKEFKCKVVTDTALPFLPKLQASPRSAPIYDVLHTNSNEQWAAVEMGLVEPKIDPKLVPN
HHCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCHHCCC
LADVYPFAISDKIVGVSIFTSAIGLGMRTDKGYPTPTSWKDLWDERYNGVRGGYVIPVNS
HHHHCCHHHCCCEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEHHH
LGQAFVPMCGMLYGKGPTDLDAAYAALEKVKPIKLVDFTGAMEKMLLSGEVGMCIIHDSG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCEEEEEECC
INRYYDDKPPTAFVAPKEGVLALEQVLTITPGSKVKELANAYVNYMLSPDVQKKLAQAVW
CCCCCCCCCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHE
YSPSNKMVKLDEPYASRLLTTPEKVASLIQVDWKWYNAHKDAIDSRVNRIFRA
ECCCCCEEEECCHHHHHHCCCHHHHHHHHHHHHHEECHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA