Click here to switch to the map view.

The map label for this gene is potH [H]

Identifier: 158424346

GI number: 158424346

Start: 3091728

End: 3092564

Strand: Direct

Name: potH [H]

Synonym: AZC_2722

Alternate gene names: 158424346

Gene position: 3091728-3092564 (Clockwise)

Preceding gene: 158424345

Following gene: 158424347

Centisome position: 57.58

GC content: 62.84

Gene sequence:

>837_bases
ATGGGCCTTGCGCTGCTGCCGAGCGCGCTGTTCTTCGCCCTCTTCTTTTTCCTGCCCATCGGCCTCATGGCGGTGATGAG
CTTTCTCACCGGCAATCCGGTGGTGATGCCCAATGTCACCGCCACCACCCGCCATTACGAGCGCATTCTCGACGATCCCT
ATTATTTCGAAGTGATGTGGACCACCGTTCGCATCGGCCTCTACACCACCGCCGCGACGCTCATCCTCGGCTATCCCCTC
GCCCACCTGATGGCGCGCATCAAGAGCCGCTCCTTGCACGCCCTGCTGCTCATGGCGGTGCTGATGCCCATGCTCACCGG
CATCGTGGTGCGCACCTTCGCCTGGATGGCGCTGCTCTCCGACAAGGGCGTCGTGAACCAGATCCTTCAGGCCCTCGGCT
TCGCACCCATGCAGTTGATGTATAACGAGTTCGGCATCGTGCTCGGCCTCGTGCACATCTATCTGCCCTTCATGATCCTG
ACGCTCACCGGCGTCATCGGCCGCATCGACGAGCGGCTGGAGCAGGCGGCGCAGAACCTCGGGGCAAGCCCGATCCGCGC
CTTCATCGAAGTGACGCTGCCGCTCAGCGTGCCCGGCATCCTCGCCGGCTCACTGCTGGTCTTTGCGCTGGCCATCAGCG
CCTATGTGACGCCGATCCTGATGGGTGGCTTCCAGATCATGACGCTGCCGATCCTGATCTATCAGCAGATTTCCGCGAAC
TTCAACATCGGCTTCGCGGCGGCGCTCGGCATGGTGCTGCTCACCCTCTCGCTGATCCTCGTGTTCGCCTACAACCACAT
TCTCGCGGCCGTCAGCGGCCAGCGGGACCTTGCGTGA

Upstream 100 bases:

>100_bases
TGGAGGCCATGGCCCGCAGTGTCGGACGGGCGATCGGCGATGCGGTGTGGGGCCTTGAGACGGGAGAACAGGCGCGATGA
ACCGCACGGGCGCCAATGTG

Downstream 100 bases:

>100_bases
GGCCCGGCCATGCTCTCCCTCAAAATCGGCCGCTTCCTCTATCTCGCAGCCAACTGGGCGGTGCTGGCCTTTCTGCTGCT
GCCCATCGCCATCATCTTCG

Product: ABC transporter permease

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MGLALLPSALFFALFFFLPIGLMAVMSFLTGNPVVMPNVTATTRHYERILDDPYYFEVMWTTVRIGLYTTAATLILGYPL
AHLMARIKSRSLHALLLMAVLMPMLTGIVVRTFAWMALLSDKGVVNQILQALGFAPMQLMYNEFGIVLGLVHIYLPFMIL
TLTGVIGRIDERLEQAAQNLGASPIRAFIEVTLPLSVPGILAGSLLVFALAISAYVTPILMGGFQIMTLPILIYQQISAN
FNIGFAAALGMVLLTLSLILVFAYNHILAAVSGQRDLA

Sequences:

>Translated_278_residues
MGLALLPSALFFALFFFLPIGLMAVMSFLTGNPVVMPNVTATTRHYERILDDPYYFEVMWTTVRIGLYTTAATLILGYPL
AHLMARIKSRSLHALLLMAVLMPMLTGIVVRTFAWMALLSDKGVVNQILQALGFAPMQLMYNEFGIVLGLVHIYLPFMIL
TLTGVIGRIDERLEQAAQNLGASPIRAFIEVTLPLSVPGILAGSLLVFALAISAYVTPILMGGFQIMTLPILIYQQISAN
FNIGFAAALGMVLLTLSLILVFAYNHILAAVSGQRDLA
>Mature_277_residues
GLALLPSALFFALFFFLPIGLMAVMSFLTGNPVVMPNVTATTRHYERILDDPYYFEVMWTTVRIGLYTTAATLILGYPLA
HLMARIKSRSLHALLLMAVLMPMLTGIVVRTFAWMALLSDKGVVNQILQALGFAPMQLMYNEFGIVLGLVHIYLPFMILT
LTGVIGRIDERLEQAAQNLGASPIRAFIEVTLPLSVPGILAGSLLVFALAISAYVTPILMGGFQIMTLPILIYQQISANF
NIGFAAALGMVLLTLSLILVFAYNHILAAVSGQRDLA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=240, Percent_Identity=31.25, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1787369, Length=215, Percent_Identity=37.2093023255814, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1787713, Length=231, Percent_Identity=33.7662337662338, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30354; Mature: 30223

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GLALLPSALFFALFFFLPIGLMAVMSFLTGNPVVMPNVTATTRHYERILDDPYYFEVMW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHCCCHHHHHHH
TTVRIGLYTTAATLILGYPLAHLMARIKSRSLHALLLMAVLMPMLTGIVVRTFAWMALLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DKGVVNQILQALGFAPMQLMYNEFGIVLGLVHIYLPFMILTLTGVIGRIDERLEQAAQNL
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GASPIRAFIEVTLPLSVPGILAGSLLVFALAISAYVTPILMGGFQIMTLPILIYQQISAN
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
FNIGFAAALGMVLLTLSLILVFAYNHILAAVSGQRDLA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]