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The map label for this gene is gsiB [H]

Identifier: 158424322

GI number: 158424322

Start: 3057816

End: 3059348

Strand: Reverse

Name: gsiB [H]

Synonym: AZC_2698

Alternate gene names: 158424322

Gene position: 3059348-3057816 (Counterclockwise)

Preceding gene: 158424323

Following gene: 158424321

Centisome position: 56.97

GC content: 65.23

Gene sequence:

>1533_bases
ATGGCGATGGCATCCCGGACGGGCAGGCGGACAGGATTTCACGCGGCGCGGCTGGGTCGGGGGCTGCTGGTCGCTGCCGC
TGTGTTGGGGCTGGGCGGCACCGCCCAGGCGGCGAAGGACCGACTGGTCGTGGGCATGCCCGTCGAGCCCACCGGCCTTG
ATCCGACGGTGGCAGCGCCCGTGTCCATCGGGCAGGTCACCTGGCAGAATGTCTATCAGGGCCTGACCCGCGTGGACCGC
CACGGCAAGGTCCAGCCGCAACTCGCGTCGGGCTGGACGGTATCGCCCGACGGCCTCGTCTACACCTTTACCTTACGGGA
TGGCGTGGCCTTCCACGACGGCACGCCCTTCAACGCCGCCGCTGCCAAGTTCAGCCTTGACCGGGCGCGCGGCGCGGATT
CGACCAATCCCCAGAAGCCCTATTTCGAGCCGATCGCATCCGTGGACGCGCCGGATGCGAAGACGCTGGTGGTCACCCTC
AAGCGGCCGGTCAGCGATCTGACCTATCGTCTCGGAATGCCCGCGGCGGTGATGGTCGCGTCGAATTCCGCGGACGCCAA
CCGTACGACGCCCGTGGGCACCGGGCCCTTCCGGTTCAAGGCCTGGGCCAAGGGTGACCGGGTGGAACTGGAGCGTTTCG
CCGATTATTGGGACAAGGCCCACGCGCCGGCTCTGGCGCAGGTGACCTTCCGCTTCCTCGCCGATCCGCAGGCGCAGGCG
GCGGCGCTGCGGGCCGGGGATGTGGACGCCTTCCCCGAGTTCAGCGCGCCGGAAATCTATACGGATTTCCAGAAGGACAA
GAAATTCAGCACCGTCGTGGGCAACACCGAGCTGAAGATGGTGGCGGGCATGAACAATGCCCGCAAGCCCTTCAACGATG
TGCGGGTGCGCAAGGCGCTGATGATGGCGGTGGATCGCAAGGCGCTGGTGGAGGGCGCGTGGTCCGGTTTCGGCACGCCC
ATCGGCAGCCACTACACGCCGAACGATCCCGGTTTTGTCGATACGACGGGTGTGCTGCCCTATGATCCCGCCAAGGCCAA
GGCGCTGCTGGCGCAGGCGGGCTATCCGAACGGCTTCACCTTCACTCTGAAGACGCCGCAGATGGCCTATCTGTCGCGCT
CGGGGGAAGTGCTTCAGGCCATGCTGGCGGATGTGGGTGTGACGGTGAAGATCGAGCCCACGGAATTTCCGGCCAAATGG
GTGCAGGAGGTCTTCAAGGACAAGGCCTATGACATGACTGTCATGACCCATGCGGAGCCACTCGACATCGATATCTACGC
GCGCCCCAATTATTACTTCAATTACAACAGTCCAAAATTCAACGCGGCCTACACGGCCGCCGCTACGGCAACGGACGACG
CCGCTCGCACCAAGGCCATTGGCGAGGCCCAGCGCATCCTTGCTGAGGAGGTTCCGGCGCTGTTCCTGTTCGTCATGCCC
AAGCTCGGTGTCTGGAGCGCCAAGGTGGAGGGCATGTGGGAGAGCGAGCCCGTGCCCTCCAATGACCTGACCGAGGTGCG
CTGGCGTGACTGA

Upstream 100 bases:

>100_bases
CGGTCTTCCGAAGGTCGTTGACGATGGCCGTGCCGGCTTGCAGTGTTTCTAGGCAGTCCGGCGTTGTGCCGAGTCGATGG
GCATTCAGGGTTGGTGAGGA

Downstream 100 bases:

>100_bases
GCCCGGCCCGGTGCACGCGAGCTGAGGCGCGCTCCATGCTGGCTTTCGCCGTGCGACGCTTTCTCGGGCTGGTGCTGACG
CTGGCAGTCATGTCGGCGGC

Product: ABC transporter substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MAMASRTGRRTGFHAARLGRGLLVAAAVLGLGGTAQAAKDRLVVGMPVEPTGLDPTVAAPVSIGQVTWQNVYQGLTRVDR
HGKVQPQLASGWTVSPDGLVYTFTLRDGVAFHDGTPFNAAAAKFSLDRARGADSTNPQKPYFEPIASVDAPDAKTLVVTL
KRPVSDLTYRLGMPAAVMVASNSADANRTTPVGTGPFRFKAWAKGDRVELERFADYWDKAHAPALAQVTFRFLADPQAQA
AALRAGDVDAFPEFSAPEIYTDFQKDKKFSTVVGNTELKMVAGMNNARKPFNDVRVRKALMMAVDRKALVEGAWSGFGTP
IGSHYTPNDPGFVDTTGVLPYDPAKAKALLAQAGYPNGFTFTLKTPQMAYLSRSGEVLQAMLADVGVTVKIEPTEFPAKW
VQEVFKDKAYDMTVMTHAEPLDIDIYARPNYYFNYNSPKFNAAYTAAATATDDAARTKAIGEAQRILAEEVPALFLFVMP
KLGVWSAKVEGMWESEPVPSNDLTEVRWRD

Sequences:

>Translated_510_residues
MAMASRTGRRTGFHAARLGRGLLVAAAVLGLGGTAQAAKDRLVVGMPVEPTGLDPTVAAPVSIGQVTWQNVYQGLTRVDR
HGKVQPQLASGWTVSPDGLVYTFTLRDGVAFHDGTPFNAAAAKFSLDRARGADSTNPQKPYFEPIASVDAPDAKTLVVTL
KRPVSDLTYRLGMPAAVMVASNSADANRTTPVGTGPFRFKAWAKGDRVELERFADYWDKAHAPALAQVTFRFLADPQAQA
AALRAGDVDAFPEFSAPEIYTDFQKDKKFSTVVGNTELKMVAGMNNARKPFNDVRVRKALMMAVDRKALVEGAWSGFGTP
IGSHYTPNDPGFVDTTGVLPYDPAKAKALLAQAGYPNGFTFTLKTPQMAYLSRSGEVLQAMLADVGVTVKIEPTEFPAKW
VQEVFKDKAYDMTVMTHAEPLDIDIYARPNYYFNYNSPKFNAAYTAAATATDDAARTKAIGEAQRILAEEVPALFLFVMP
KLGVWSAKVEGMWESEPVPSNDLTEVRWRD
>Mature_509_residues
AMASRTGRRTGFHAARLGRGLLVAAAVLGLGGTAQAAKDRLVVGMPVEPTGLDPTVAAPVSIGQVTWQNVYQGLTRVDRH
GKVQPQLASGWTVSPDGLVYTFTLRDGVAFHDGTPFNAAAAKFSLDRARGADSTNPQKPYFEPIASVDAPDAKTLVVTLK
RPVSDLTYRLGMPAAVMVASNSADANRTTPVGTGPFRFKAWAKGDRVELERFADYWDKAHAPALAQVTFRFLADPQAQAA
ALRAGDVDAFPEFSAPEIYTDFQKDKKFSTVVGNTELKMVAGMNNARKPFNDVRVRKALMMAVDRKALVEGAWSGFGTPI
GSHYTPNDPGFVDTTGVLPYDPAKAKALLAQAGYPNGFTFTLKTPQMAYLSRSGEVLQAMLADVGVTVKIEPTEFPAKWV
QEVFKDKAYDMTVMTHAEPLDIDIYARPNYYFNYNSPKFNAAYTAAATATDDAARTKAIGEAQRILAEEVPALFLFVMPK
LGVWSAKVEGMWESEPVPSNDLTEVRWRD

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=514, Percent_Identity=31.3229571984436, Blast_Score=204, Evalue=2e-53,
Organism=Escherichia coli, GI1789966, Length=350, Percent_Identity=30.2857142857143, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789887, Length=413, Percent_Identity=29.7820823244552, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1787762, Length=471, Percent_Identity=26.1146496815287, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1787495, Length=543, Percent_Identity=25.9668508287293, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87081878, Length=509, Percent_Identity=25.7367387033399, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787551, Length=487, Percent_Identity=25.6673511293634, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1789397, Length=482, Percent_Identity=24.2738589211618, Blast_Score=99, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 55386; Mature: 55255

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMASRTGRRTGFHAARLGRGLLVAAAVLGLGGTAQAAKDRLVVGMPVEPTGLDPTVAAP
CCCCCCCCCCCCCHHHHHCCHHHHHHHHHCCCCCCHHCCCCEEEECCCCCCCCCCCCCCC
VSIGQVTWQNVYQGLTRVDRHGKVQPQLASGWTVSPDGLVYTFTLRDGVAFHDGTPFNAA
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCEEEEEEECCCEEECCCCCCCHH
AAKFSLDRARGADSTNPQKPYFEPIASVDAPDAKTLVVTLKRPVSDLTYRLGMPAAVMVA
HHHEEHHHHCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEECCCHHHHHHHCCCCEEEEEE
SNSADANRTTPVGTGPFRFKAWAKGDRVELERFADYWDKAHAPALAQVTFRFLADPQAQA
CCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHH
AALRAGDVDAFPEFSAPEIYTDFQKDKKFSTVVGNTELKMVAGMNNARKPFNDVRVRKAL
HHHCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHHH
MMAVDRKALVEGAWSGFGTPIGSHYTPNDPGFVDTTGVLPYDPAKAKALLAQAGYPNGFT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHCCCCCCEE
FTLKTPQMAYLSRSGEVLQAMLADVGVTVKIEPTEFPAKWVQEVFKDKAYDMTVMTHAEP
EEECCCCCEEHHCCHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCC
LDIDIYARPNYYFNYNSPKFNAAYTAAATATDDAARTKAIGEAQRILAEEVPALFLFVMP
CEEEEEECCCEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KLGVWSAKVEGMWESEPVPSNDLTEVRWRD
CCCCEEHEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AMASRTGRRTGFHAARLGRGLLVAAAVLGLGGTAQAAKDRLVVGMPVEPTGLDPTVAAP
CCCCCCCCCCCCHHHHHCCHHHHHHHHHCCCCCCHHCCCCEEEECCCCCCCCCCCCCCC
VSIGQVTWQNVYQGLTRVDRHGKVQPQLASGWTVSPDGLVYTFTLRDGVAFHDGTPFNAA
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCEEEEEEECCCEEECCCCCCCHH
AAKFSLDRARGADSTNPQKPYFEPIASVDAPDAKTLVVTLKRPVSDLTYRLGMPAAVMVA
HHHEEHHHHCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEECCCHHHHHHHCCCCEEEEEE
SNSADANRTTPVGTGPFRFKAWAKGDRVELERFADYWDKAHAPALAQVTFRFLADPQAQA
CCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHH
AALRAGDVDAFPEFSAPEIYTDFQKDKKFSTVVGNTELKMVAGMNNARKPFNDVRVRKAL
HHHCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHHH
MMAVDRKALVEGAWSGFGTPIGSHYTPNDPGFVDTTGVLPYDPAKAKALLAQAGYPNGFT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHCCCCCCEE
FTLKTPQMAYLSRSGEVLQAMLADVGVTVKIEPTEFPAKWVQEVFKDKAYDMTVMTHAEP
EEECCCCCEEHHCCHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCC
LDIDIYARPNYYFNYNSPKFNAAYTAAATATDDAARTKAIGEAQRILAEEVPALFLFVMP
CEEEEEECCCEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KLGVWSAKVEGMWESEPVPSNDLTEVRWRD
CCCCEEHEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA