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The map label for this gene is dctA [H]

Identifier: 158424297

GI number: 158424297

Start: 3031943

End: 3033301

Strand: Direct

Name: dctA [H]

Synonym: AZC_2673

Alternate gene names: 158424297

Gene position: 3031943-3033301 (Clockwise)

Preceding gene: 158424296

Following gene: 158424298

Centisome position: 56.46

GC content: 66.0

Gene sequence:

>1359_bases
ATGCCGGCCCATGCAGAGCCACAGCCCCTCGCCGGGCGAAAGCCGAAACGCTTCTACGAGCAGCTTTATGTCCAGGTGCT
GATCGGCGTCGCCGCCGGCATCGCGCTCGGGCACTTCTACCCGCAGATCGCGCCCATGGCGAAGCCCCTGGGCGATGGCT
TCATCAAGCTCGTGAAGATGATGATCGTGCCGATCGTCTTCTGCACCATCGTGGTGGGCGTGGCCGGCGTGCATGCGGAC
CGCAAGATCGGCGCCTCGCTGCTCAAGACCATGCTCTTGTTCTACGGCCTCACCATCATCGGCCTGATGACCGGCCTGAT
CGCGGTCGAGACCATCCAGCCCGGCGCCGGCATGCACATGGACGTGCATGCCATCGACGCCAAGGAAGCCGCCCGCTACG
CTGCGGGCGCCAAGAAGCTCGATACCGTGGACGTGCTGCTGCACATCATCCCGCCCAGCTTCTTCACGCCCTTCGCGGAG
GGTGAGGTGCTGCCGGTGCTGTTCATCGCCCTCATCACCGGGTTCGGCCTGCGCAAGGCGGGCGTGTCCGGCGAGCCGTT
CCTGCGCGGCGTCGAAGCCTTCTCCCGCGCCCTGTTCGCCGCTTTCGGCTACATGATGCGGCTTGCCCCCATCGGCGCCT
TCGGAGCCATCGCCTTCATCGTCGCCAAGAGCGGCCTGAAGACCATCGGCAACCTCGGCCTGCTGATCTTCACGCTTTAT
GTGGCCTGCGGTTTCTTCGTCTTCGTCGTCCTCTGGGGACTGGCGCGGTTCCACGGATTCAGCCTCTTCCGGCTGCTGCG
GTACTTCCGCGAGGAACTGCTGATCGTGCTCGGCACCTCGTCCACCGAGCCCGTGCTTCCCGCTGTGCTCTACAAGCTGG
AACGCCTCGGCTGCTCCAAGGGCTCGGTCGGCCTCACCCTGCCGCTCGGCTATTCCTTCAATCTCGACGGCACGGCCATC
TATCTGACCCTCGCCTCGCTGTTCCTGGCGCAGGCCATGGATATCCATCTGAGCGCCTGGCAGGTCGGCTCCATGATCTT
CGTGATGCTGCTGACGTCGAAGGGCGCCGCCGGTGTGACTGGCAGCGGGTTCGCCGCCCTCGTCGCGACGCTGGCGGCGA
TGCCGGACACGGTGCCCGTGGCGGGCGTGGTGATGATCGCCGGCATCGACCGCTTCATGTCGGAAGCCCGGGCCCTGACC
AGCCTTGTGAGCAACATGGTCGCCTGTATCGTCATCGCCATCTGGGACGGCGAATGCGACCGCTCCGTCCTCCTCGCCGA
ACTGGCGCAGGGCAAGCCCACGGAGCGTCCGGCGGTCGAAAGCACCGGCGCCGTTGCGGAACTGCCCCAGACCGTCTGA

Upstream 100 bases:

>100_bases
GTTTTCGCGCGCATCGACCAAGATGAGGGCGCCCGCGCCGCCGCCTGACGGCGCAGCGCCCGCGCAGAACAAGCAAAAGA
AGACCAACGGAGGAAAGTTC

Downstream 100 bases:

>100_bases
TCGACGACCGGTTTCCTTCCTCCCTTCCTGCCGCGGGATGCGGGGCGCTTCGGAAACGGAGCGCCCCGCAGTCTGTGGCA
GTCTTCTTTGGGCGGTCCGC

Product: C4-dicarboxylate transporter

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MPAHAEPQPLAGRKPKRFYEQLYVQVLIGVAAGIALGHFYPQIAPMAKPLGDGFIKLVKMMIVPIVFCTIVVGVAGVHAD
RKIGASLLKTMLLFYGLTIIGLMTGLIAVETIQPGAGMHMDVHAIDAKEAARYAAGAKKLDTVDVLLHIIPPSFFTPFAE
GEVLPVLFIALITGFGLRKAGVSGEPFLRGVEAFSRALFAAFGYMMRLAPIGAFGAIAFIVAKSGLKTIGNLGLLIFTLY
VACGFFVFVVLWGLARFHGFSLFRLLRYFREELLIVLGTSSTEPVLPAVLYKLERLGCSKGSVGLTLPLGYSFNLDGTAI
YLTLASLFLAQAMDIHLSAWQVGSMIFVMLLTSKGAAGVTGSGFAALVATLAAMPDTVPVAGVVMIAGIDRFMSEARALT
SLVSNMVACIVIAIWDGECDRSVLLAELAQGKPTERPAVESTGAVAELPQTV

Sequences:

>Translated_452_residues
MPAHAEPQPLAGRKPKRFYEQLYVQVLIGVAAGIALGHFYPQIAPMAKPLGDGFIKLVKMMIVPIVFCTIVVGVAGVHAD
RKIGASLLKTMLLFYGLTIIGLMTGLIAVETIQPGAGMHMDVHAIDAKEAARYAAGAKKLDTVDVLLHIIPPSFFTPFAE
GEVLPVLFIALITGFGLRKAGVSGEPFLRGVEAFSRALFAAFGYMMRLAPIGAFGAIAFIVAKSGLKTIGNLGLLIFTLY
VACGFFVFVVLWGLARFHGFSLFRLLRYFREELLIVLGTSSTEPVLPAVLYKLERLGCSKGSVGLTLPLGYSFNLDGTAI
YLTLASLFLAQAMDIHLSAWQVGSMIFVMLLTSKGAAGVTGSGFAALVATLAAMPDTVPVAGVVMIAGIDRFMSEARALT
SLVSNMVACIVIAIWDGECDRSVLLAELAQGKPTERPAVESTGAVAELPQTV
>Mature_451_residues
PAHAEPQPLAGRKPKRFYEQLYVQVLIGVAAGIALGHFYPQIAPMAKPLGDGFIKLVKMMIVPIVFCTIVVGVAGVHADR
KIGASLLKTMLLFYGLTIIGLMTGLIAVETIQPGAGMHMDVHAIDAKEAARYAAGAKKLDTVDVLLHIIPPSFFTPFAEG
EVLPVLFIALITGFGLRKAGVSGEPFLRGVEAFSRALFAAFGYMMRLAPIGAFGAIAFIVAKSGLKTIGNLGLLIFTLYV
ACGFFVFVVLWGLARFHGFSLFRLLRYFREELLIVLGTSSTEPVLPAVLYKLERLGCSKGSVGLTLPLGYSFNLDGTAIY
LTLASLFLAQAMDIHLSAWQVGSMIFVMLLTSKGAAGVTGSGFAALVATLAAMPDTVPVAGVVMIAGIDRFMSEARALTS
LVSNMVACIVIAIWDGECDRSVLLAELAQGKPTERPAVESTGAVAELPQTV

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=495, Percent_Identity=22.6262626262626, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI4827012, Length=270, Percent_Identity=26.2962962962963, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI21314632, Length=396, Percent_Identity=22.7272727272727, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI5032093, Length=323, Percent_Identity=25.3869969040248, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI223468564, Length=246, Percent_Identity=27.2357723577236, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI223468566, Length=246, Percent_Identity=27.2357723577236, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI194239697, Length=268, Percent_Identity=24.6268656716418, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI66773030, Length=474, Percent_Identity=22.9957805907173, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1789947, Length=419, Percent_Identity=49.6420047732697, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI1790514, Length=423, Percent_Identity=34.51536643026, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI1788024, Length=400, Percent_Identity=23.25, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17537407, Length=393, Percent_Identity=24.9363867684478, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71983099, Length=137, Percent_Identity=32.1167883211679, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI71983106, Length=137, Percent_Identity=32.1167883211679, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71996953, Length=457, Percent_Identity=23.4135667396061, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24583025, Length=406, Percent_Identity=21.6748768472906, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17137668, Length=406, Percent_Identity=21.6748768472906, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583023, Length=406, Percent_Identity=21.6748768472906, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 48001; Mature: 47870

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAHAEPQPLAGRKPKRFYEQLYVQVLIGVAAGIALGHFYPQIAPMAKPLGDGFIKLVKM
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
MIVPIVFCTIVVGVAGVHADRKIGASLLKTMLLFYGLTIIGLMTGLIAVETIQPGAGMHM
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEE
DVHAIDAKEAARYAAGAKKLDTVDVLLHIIPPSFFTPFAEGEVLPVLFIALITGFGLRKA
EEEECCHHHHHHHHHCCCHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
GVSGEPFLRGVEAFSRALFAAFGYMMRLAPIGAFGAIAFIVAKSGLKTIGNLGLLIFTLY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VACGFFVFVVLWGLARFHGFSLFRLLRYFREELLIVLGTSSTEPVLPAVLYKLERLGCSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHCCCC
GSVGLTLPLGYSFNLDGTAIYLTLASLFLAQAMDIHLSAWQVGSMIFVMLLTSKGAAGVT
CCCCEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GSGFAALVATLAAMPDTVPVAGVVMIAGIDRFMSEARALTSLVSNMVACIVIAIWDGECD
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
RSVLLAELAQGKPTERPAVESTGAVAELPQTV
HHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCC
>Mature Secondary Structure 
PAHAEPQPLAGRKPKRFYEQLYVQVLIGVAAGIALGHFYPQIAPMAKPLGDGFIKLVKM
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
MIVPIVFCTIVVGVAGVHADRKIGASLLKTMLLFYGLTIIGLMTGLIAVETIQPGAGMHM
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEE
DVHAIDAKEAARYAAGAKKLDTVDVLLHIIPPSFFTPFAEGEVLPVLFIALITGFGLRKA
EEEECCHHHHHHHHHCCCHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
GVSGEPFLRGVEAFSRALFAAFGYMMRLAPIGAFGAIAFIVAKSGLKTIGNLGLLIFTLY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VACGFFVFVVLWGLARFHGFSLFRLLRYFREELLIVLGTSSTEPVLPAVLYKLERLGCSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHCCCC
GSVGLTLPLGYSFNLDGTAIYLTLASLFLAQAMDIHLSAWQVGSMIFVMLLTSKGAAGVT
CCCCEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GSGFAALVATLAAMPDTVPVAGVVMIAGIDRFMSEARALTSLVSNMVACIVIAIWDGECD
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
RSVLLAELAQGKPTERPAVESTGAVAELPQTV
HHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA