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The map label for this gene is gatA [H]

Identifier: 158424123

GI number: 158424123

Start: 2833180

End: 2834448

Strand: Direct

Name: gatA [H]

Synonym: AZC_2499

Alternate gene names: 158424123

Gene position: 2833180-2834448 (Clockwise)

Preceding gene: 158424122

Following gene: 158424124

Centisome position: 52.76

GC content: 71.24

Gene sequence:

>1269_bases
ATGACTGCTTTAGGTGCACTGCGGGAGAAGGGGACGACCGAAGGCGACGCCATCGCCTCCGCCCGCGCCCGGTGCGCAGC
AGCCACCCCCTCCAATGCTTTCGTCGCGCTTCTGGACCCCGCACCGGGCGGCCGCTTTCCTTTCGCGGTGAAGGACATGG
TGGAGGTGGCCGGCCACGCCCCCACCTATGGCCTCGCCCATTCGCAGGGCTCGCCCCGCACCGCTTCCGCCCCCGTCATC
GGTCTGCTGGAAGCCGCAGGCGGACGCGCCGTGGCGCTGGCGCAGATGACGCCGCTGGCCTTCGATCCGTCCGGCGACAA
CCGCTGGCAGGGGCGCCCGCTCAACCCCTGGAACACTGACCGCATCTGCGGCGGCTCGTCCTCCGGCTCGGCGGTGGCGG
TTGCGCTCGGCGCGGTGCCGCTGGCGCTGGGATCGGACACGGCGGGCTCATTGCGCATCCCCGCCCACTGCTGCGGCGTG
CTGTCCTGGAAGGCCACCGCCGGGCTCATTCCGCTGGACGGGGCACTCACCCTCGCGCCGACCCTCGACACGCTGGGCTT
CCTCGGCATTGATGCGGACTGGCTGGACAGTGCCGCCGCGGTTTTCGCGCCGGTCGCCAGCGATGCCACCCGCCGGATCG
GCCTCGCAAGCGACCTGCTGGAGGCCTGCGACGCGGATGTGGCGGCAATCTTCCGCACGCAAGCCGATCACGCGCGGACG
TCTTTTGCCACGACCGACGTCCCCGTCGCGCCGCTCATCGCTGCAACCGACGGCCCGGTGCTCGACCTGCTGATCGGAGA
AAGCGCCCGGTCGCTGGCGCACCTGCCGGAAGCGGAAGCCGATCCGCTCTTTGCCAAGCGCCTCGCCAAAGGCCGCGCCC
TTTCGGACGCGCATCTCGCCGCCTGCCGCGACCAGTTACGCGCGGCGGAGGCCATGGCGGCGGATATCTTCGCCACCTGC
GACGCCCTCCTGCTGCCGGCCATGCCCTGCGCGACGCCCACCATCGCCGCCTGCGATCCGGCAACGCCCGGCTTCTCCGG
CCGCACGCTCTATCGCCTCTCCGCCTTCACCCGCTTCGCCAACGGCCTCGGCCTGCCGGTCGTGACCTTTCCCATCGGCC
TTGATGCCAACGGAATGCCCGTGGCGGCGCAACTGGTGGGGCCCCGCGGCAGTGACCGCGCCCTCATCGCCGGCGTGCAC
CATCTCATGGCCGAAAACACTTGGCCGCCCCACGACAAAGATCCGTCCCGGAAGGAGCCCCTCGCGTGA

Upstream 100 bases:

>100_bases
CGAGCTAGCGCCTGCGCATTTCGCGAAATCTGAATGCTTTCTTTTCTAATTCCGCGAAGCACGCACCTGAGCTTACCCTG
CCTGTGCTGGAGAAACAGGC

Downstream 100 bases:

>100_bases
GCGCCACCGCACAGGCCCCCGCGGCCGACCGTCCGGCCGCCTCCACGGCCCTCGCCAACCTGCGCATCCTCGACCTGACG
CGCGTGCGGGCCGGGCCGAC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MTALGALREKGTTEGDAIASARARCAAATPSNAFVALLDPAPGGRFPFAVKDMVEVAGHAPTYGLAHSQGSPRTASAPVI
GLLEAAGGRAVALAQMTPLAFDPSGDNRWQGRPLNPWNTDRICGGSSSGSAVAVALGAVPLALGSDTAGSLRIPAHCCGV
LSWKATAGLIPLDGALTLAPTLDTLGFLGIDADWLDSAAAVFAPVASDATRRIGLASDLLEACDADVAAIFRTQADHART
SFATTDVPVAPLIAATDGPVLDLLIGESARSLAHLPEAEADPLFAKRLAKGRALSDAHLAACRDQLRAAEAMAADIFATC
DALLLPAMPCATPTIAACDPATPGFSGRTLYRLSAFTRFANGLGLPVVTFPIGLDANGMPVAAQLVGPRGSDRALIAGVH
HLMAENTWPPHDKDPSRKEPLA

Sequences:

>Translated_422_residues
MTALGALREKGTTEGDAIASARARCAAATPSNAFVALLDPAPGGRFPFAVKDMVEVAGHAPTYGLAHSQGSPRTASAPVI
GLLEAAGGRAVALAQMTPLAFDPSGDNRWQGRPLNPWNTDRICGGSSSGSAVAVALGAVPLALGSDTAGSLRIPAHCCGV
LSWKATAGLIPLDGALTLAPTLDTLGFLGIDADWLDSAAAVFAPVASDATRRIGLASDLLEACDADVAAIFRTQADHART
SFATTDVPVAPLIAATDGPVLDLLIGESARSLAHLPEAEADPLFAKRLAKGRALSDAHLAACRDQLRAAEAMAADIFATC
DALLLPAMPCATPTIAACDPATPGFSGRTLYRLSAFTRFANGLGLPVVTFPIGLDANGMPVAAQLVGPRGSDRALIAGVH
HLMAENTWPPHDKDPSRKEPLA
>Mature_421_residues
TALGALREKGTTEGDAIASARARCAAATPSNAFVALLDPAPGGRFPFAVKDMVEVAGHAPTYGLAHSQGSPRTASAPVIG
LLEAAGGRAVALAQMTPLAFDPSGDNRWQGRPLNPWNTDRICGGSSSGSAVAVALGAVPLALGSDTAGSLRIPAHCCGVL
SWKATAGLIPLDGALTLAPTLDTLGFLGIDADWLDSAAAVFAPVASDATRRIGLASDLLEACDADVAAIFRTQADHARTS
FATTDVPVAPLIAATDGPVLDLLIGESARSLAHLPEAEADPLFAKRLAKGRALSDAHLAACRDQLRAAEAMAADIFATCD
ALLLPAMPCATPTIAACDPATPGFSGRTLYRLSAFTRFANGLGLPVVTFPIGLDANGMPVAAQLVGPRGSDRALIAGVHH
LMAENTWPPHDKDPSRKEPLA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=404, Percent_Identity=24.009900990099, Blast_Score=79, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17537465, Length=460, Percent_Identity=25.6521739130435, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17556264, Length=120, Percent_Identity=35, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21356731, Length=394, Percent_Identity=25.1269035532995, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24652985, Length=415, Percent_Identity=23.855421686747, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19922090, Length=415, Percent_Identity=23.855421686747, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24652981, Length=415, Percent_Identity=23.855421686747, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24652983, Length=415, Percent_Identity=23.855421686747, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24644968, Length=140, Percent_Identity=33.5714285714286, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 43073; Mature: 42942

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALGALREKGTTEGDAIASARARCAAATPSNAFVALLDPAPGGRFPFAVKDMVEVAGHA
CCCHHHHHHCCCCCHHHHHHHHHHHEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCC
PTYGLAHSQGSPRTASAPVIGLLEAAGGRAVALAQMTPLAFDPSGDNRWQGRPLNPWNTD
CCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCEEECCCCCCCCCCCCCCCCCCC
RICGGSSSGSAVAVALGAVPLALGSDTAGSLRIPAHCCGVLSWKATAGLIPLDGALTLAP
CCCCCCCCCCEEEEEECCEEEEECCCCCCCEECCHHHHHHEEHHHHCCCEECCCCEEECC
TLDTLGFLGIDADWLDSAAAVFAPVASDATRRIGLASDLLEACDADVAAIFRTQADHART
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
SFATTDVPVAPLIAATDGPVLDLLIGESARSLAHLPEAEADPLFAKRLAKGRALSDAHLA
CCCCCCCCCCCEEEECCCCEEEEEECCHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHH
ACRDQLRAAEAMAADIFATCDALLLPAMPCATPTIAACDPATPGFSGRTLYRLSAFTRFA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCEEHHHHHHHHHH
NGLGLPVVTFPIGLDANGMPVAAQLVGPRGSDRALIAGVHHLMAENTWPPHDKDPSRKEP
CCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
LA
CC
>Mature Secondary Structure 
TALGALREKGTTEGDAIASARARCAAATPSNAFVALLDPAPGGRFPFAVKDMVEVAGHA
CCHHHHHHCCCCCHHHHHHHHHHHEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCC
PTYGLAHSQGSPRTASAPVIGLLEAAGGRAVALAQMTPLAFDPSGDNRWQGRPLNPWNTD
CCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCEEECCCCCCCCCCCCCCCCCCC
RICGGSSSGSAVAVALGAVPLALGSDTAGSLRIPAHCCGVLSWKATAGLIPLDGALTLAP
CCCCCCCCCCEEEEEECCEEEEECCCCCCCEECCHHHHHHEEHHHHCCCEECCCCEEECC
TLDTLGFLGIDADWLDSAAAVFAPVASDATRRIGLASDLLEACDADVAAIFRTQADHART
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
SFATTDVPVAPLIAATDGPVLDLLIGESARSLAHLPEAEADPLFAKRLAKGRALSDAHLA
CCCCCCCCCCCEEEECCCCEEEEEECCHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHH
ACRDQLRAAEAMAADIFATCDALLLPAMPCATPTIAACDPATPGFSGRTLYRLSAFTRFA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCEEHHHHHHHHHH
NGLGLPVVTFPIGLDANGMPVAAQLVGPRGSDRALIAGVHHLMAENTWPPHDKDPSRKEP
CCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
LA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA