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The map label for this gene is potD [H]

Identifier: 158424081

GI number: 158424081

Start: 2792142

End: 2793209

Strand: Reverse

Name: potD [H]

Synonym: AZC_2457

Alternate gene names: 158424081

Gene position: 2793209-2792142 (Counterclockwise)

Preceding gene: 158424082

Following gene: 158424080

Centisome position: 52.02

GC content: 64.7

Gene sequence:

>1068_bases
ATGACGGACAAGATCCGCCTCAGCCGCCGCTCGGCGCTCAAGGGGCTCGGGGCGCTCGCCGCCGCGCCGCTGGCGGCGCC
CGCGGTGATCACCCGCTCGCTCGCCGCGGGCGAGACGCTGGTGGTCTATAATTTCGACGGCCTGCTCGGAAAGGTCATCA
AGGATCACTGGATCGACCCCTTCACCCAGAAGACCGGCGTGAAGGTCGAGGTGCTCACCATGCAGGGTTCCTCGCCGCCT
ATGGCGAAGATCAAGGCGCAGGTGGATGCGGGCCGGCCCGATGCCGATGTCATCCCCATGCAGATGACGGACTATGTCTT
CGGCGTCCGGAACAACTTGTTCCAGACCATCGACGCGAAGGACATGCCGGAATATGCCAACCTCTATCCCAGCTACATCA
CCCCGCACGGGCCGAAGCTGGTGCTGTGGAGCTATGGCATCGGCTACAACACCGAGAAGGTGAAGACGCCGCCCACCAAA
TGGGCGGACCTCTGGGACCCGCAGTACAAGGGCAAGGTGGCGCTCAACGAGGCCCTGTTCGATCAGGCCCTCGAAATGGT
CAATCTCGTCGTGAAGGGCAAGCCCCTGCCGGTGGACGATGCGACCTTCGCCGCCCTCACCAAACTGCGGCCCAACCTTG
CCACCCTCTGGACGACCGGCGCGCAGGCGGAGCAACTGATCCGCTCGGGCGAGGTTTCGCTCATCGCCGTGTGGAACGGG
CGCGTCTATCCGCTGAAGGATCAGGGCGTGCCGATCGATTTCGTCAGCCCGCAGGAAGGTTTCTTCACGCGCGCCGATCC
CTTCTGCATCCCGCGCGGCGCCCGCAATCCGGATCTCGCCAAGGCCTTCATCAACTTCTCCTGCACCGTCGCCCCGCAGC
AGGCGATGGCCGAGAAGCTGTTCTACGCCTCGCCCAACAAGAAGGTCGTCTATTCGCCGGAGATCGCCAAGCGCGTGGTC
GTCTCGACGCCCGAGGAACTCGCCCGCGCGGTGCCGGAGGATTACGAGGCTATCGTCGACAATCTGCCCGAATGGCGCCG
GCGCTGGGATGCGTGGAAGCAGAGCTGA

Upstream 100 bases:

>100_bases
CGCCGGCCGCCTGATCCGCCGCCGGTTTCCAACCCCAACGATACCAGAACGCGCCTCAAGGCGCGCAGGCCTTCGCTCAA
CTCAAGACGGGGAACAGACA

Downstream 100 bases:

>100_bases
GCGGAGATCATCCATGGCGCTCCGCACCGGCCGGCGGATCTTCTGGTGGGGGCTCATTGCCCCCACCTTCGTCCTCTATG
CCCTCATCTTCTTCGTGCCG

Product: twin-arginine translocation pathway signal precursor

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MTDKIRLSRRSALKGLGALAAAPLAAPAVITRSLAAGETLVVYNFDGLLGKVIKDHWIDPFTQKTGVKVEVLTMQGSSPP
MAKIKAQVDAGRPDADVIPMQMTDYVFGVRNNLFQTIDAKDMPEYANLYPSYITPHGPKLVLWSYGIGYNTEKVKTPPTK
WADLWDPQYKGKVALNEALFDQALEMVNLVVKGKPLPVDDATFAALTKLRPNLATLWTTGAQAEQLIRSGEVSLIAVWNG
RVYPLKDQGVPIDFVSPQEGFFTRADPFCIPRGARNPDLAKAFINFSCTVAPQQAMAEKLFYASPNKKVVYSPEIAKRVV
VSTPEELARAVPEDYEAIVDNLPEWRRRWDAWKQS

Sequences:

>Translated_355_residues
MTDKIRLSRRSALKGLGALAAAPLAAPAVITRSLAAGETLVVYNFDGLLGKVIKDHWIDPFTQKTGVKVEVLTMQGSSPP
MAKIKAQVDAGRPDADVIPMQMTDYVFGVRNNLFQTIDAKDMPEYANLYPSYITPHGPKLVLWSYGIGYNTEKVKTPPTK
WADLWDPQYKGKVALNEALFDQALEMVNLVVKGKPLPVDDATFAALTKLRPNLATLWTTGAQAEQLIRSGEVSLIAVWNG
RVYPLKDQGVPIDFVSPQEGFFTRADPFCIPRGARNPDLAKAFINFSCTVAPQQAMAEKLFYASPNKKVVYSPEIAKRVV
VSTPEELARAVPEDYEAIVDNLPEWRRRWDAWKQS
>Mature_354_residues
TDKIRLSRRSALKGLGALAAAPLAAPAVITRSLAAGETLVVYNFDGLLGKVIKDHWIDPFTQKTGVKVEVLTMQGSSPPM
AKIKAQVDAGRPDADVIPMQMTDYVFGVRNNLFQTIDAKDMPEYANLYPSYITPHGPKLVLWSYGIGYNTEKVKTPPTKW
ADLWDPQYKGKVALNEALFDQALEMVNLVVKGKPLPVDDATFAALTKLRPNLATLWTTGAQAEQLIRSGEVSLIAVWNGR
VYPLKDQGVPIDFVSPQEGFFTRADPFCIPRGARNPDLAKAFINFSCTVAPQQAMAEKLFYASPNKKVVYSPEIAKRVVV
STPEELARAVPEDYEAIVDNLPEWRRRWDAWKQS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=273, Percent_Identity=31.1355311355311, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787078, Length=327, Percent_Identity=27.5229357798165, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39351; Mature: 39219

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKIRLSRRSALKGLGALAAAPLAAPAVITRSLAAGETLVVYNFDGLLGKVIKDHWIDP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCC
FTQKTGVKVEVLTMQGSSPPMAKIKAQVDAGRPDADVIPMQMTDYVFGVRNNLFQTIDAK
CHHCCCCEEEEEEECCCCCCHHHEEEEECCCCCCCCEECHHHHHHHHHHHHHHHHHCCCC
DMPEYANLYPSYITPHGPKLVLWSYGIGYNTEKVKTPPTKWADLWDPQYKGKVALNEALF
CCCHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHCCCCCCCCCEEHHHHHH
DQALEMVNLVVKGKPLPVDDATFAALTKLRPNLATLWTTGAQAEQLIRSGEVSLIAVWNG
HHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHEEEECCHHHHHHHHCCCEEEEEEECC
RVYPLKDQGVPIDFVSPQEGFFTRADPFCIPRGARNPDLAKAFINFSCTVAPQQAMAEKL
EEEEECCCCCCEEEECCCCCCEECCCCCCCCCCCCCHHHHHHHHCCEEEECCHHHHHHHH
FYASPNKKVVYSPEIAKRVVVSTPEELARAVPEDYEAIVDNLPEWRRRWDAWKQS
HCCCCCCEEEECHHHHHHHHCCCHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TDKIRLSRRSALKGLGALAAAPLAAPAVITRSLAAGETLVVYNFDGLLGKVIKDHWIDP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCC
FTQKTGVKVEVLTMQGSSPPMAKIKAQVDAGRPDADVIPMQMTDYVFGVRNNLFQTIDAK
CHHCCCCEEEEEEECCCCCCHHHEEEEECCCCCCCCEECHHHHHHHHHHHHHHHHHCCCC
DMPEYANLYPSYITPHGPKLVLWSYGIGYNTEKVKTPPTKWADLWDPQYKGKVALNEALF
CCCHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHCCCCCCCCCEEHHHHHH
DQALEMVNLVVKGKPLPVDDATFAALTKLRPNLATLWTTGAQAEQLIRSGEVSLIAVWNG
HHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHEEEECCHHHHHHHHCCCEEEEEEECC
RVYPLKDQGVPIDFVSPQEGFFTRADPFCIPRGARNPDLAKAFINFSCTVAPQQAMAEKL
EEEEECCCCCCEEEECCCCCCEECCCCCCCCCCCCCHHHHHHHHCCEEEECCHHHHHHHH
FYASPNKKVVYSPEIAKRVVVSTPEELARAVPEDYEAIVDNLPEWRRRWDAWKQS
HCCCCCCEEEECHHHHHHHHCCCHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]