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The map label for this gene is potH [H]

Identifier: 158424080

GI number: 158424080

Start: 2791271

End: 2792128

Strand: Reverse

Name: potH [H]

Synonym: AZC_2456

Alternate gene names: 158424080

Gene position: 2792128-2791271 (Counterclockwise)

Preceding gene: 158424081

Following gene: 158424079

Centisome position: 52.0

GC content: 66.78

Gene sequence:

>858_bases
ATGGCGCTCCGCACCGGCCGGCGGATCTTCTGGTGGGGGCTCATTGCCCCCACCTTCGTCCTCTATGCCCTCATCTTCTT
CGTGCCGCTTGGCATCCTCGGCTCCGAGAGCCTGCATGCCGGCGCCGGCGGCGGGCTGACCTCCGCCAATTACGCCGCCT
TCTTTACGGATGGCGTCACGCTTTCCATCTTCTGGCGCACGGTGCGGCTGTCGCTGCTGATCACCGCAGCGTGCATCCTG
TTTGGCTATCCGCTGGCGCTGTTCATGCGCCGCGCCGGGCCCGTCCTGCGGCTCGTGGCCCTCACCATCGTCATCTCGCC
GCTGCTCACCAGCGTCGTGGTGCGCAACGTGGCCTGGCTGCTGGTGCTGGGGCGCGAGGGCATGGTGAATGCCCTGCTGC
GCCAGAGCGGTCTCATCGATGGCCCGCTGCCGCTCCTCTACAACACGTTCGGCGTTGTCGTGGGCGTGACCCACGTCTAT
CTCGCCTTTCTGGTGATGCCGGTCTTCTCCTCGCTTCTGTCCATCGACCCGTCCGTCGAGGATGCGGCGGCGAGCCTTGG
CGCCTCGCGCACCACGGTCTTCTGGCGCGTGACCTTTCCCTTGAGCCTTCCGGGCCTCATCGCGGGGACGACGCTCGTCT
TCGTTCTGTCCATGGGCGTCTATCTGACGCCGGTGATCATGGGCGGCAGCTTCGTCACCACTTTGCCGATGGTCATGACC
GACCTCGTGCGCAACCAGTTCGACTGGAGCCGGGCGGCGGCCTTCGCCATCGTGCTCCTGGCCTTCATCCTCATCGTCCT
CGGCCTGTCGAGCCGGGCGGAGAAGCGGATCGAGCGCATGCGCGGGGAGGGCGCCTGA

Upstream 100 bases:

>100_bases
GCCCGCGCGGTGCCGGAGGATTACGAGGCTATCGTCGACAATCTGCCCGAATGGCGCCGGCGCTGGGATGCGTGGAAGCA
GAGCTGAGCGGAGATCATCC

Downstream 100 bases:

>100_bases
TGACCGACGCCAAGCGGCCCCTGGGCCTCCTCGTCAGCATCGTCGCCGTGGCGGTGATGATCTTCCTCATCCTGCCGCAG
GTGATCCTGCTCATTCAGTC

Product: ABC transporter permease

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MALRTGRRIFWWGLIAPTFVLYALIFFVPLGILGSESLHAGAGGGLTSANYAAFFTDGVTLSIFWRTVRLSLLITAACIL
FGYPLALFMRRAGPVLRLVALTIVISPLLTSVVVRNVAWLLVLGREGMVNALLRQSGLIDGPLPLLYNTFGVVVGVTHVY
LAFLVMPVFSSLLSIDPSVEDAAASLGASRTTVFWRVTFPLSLPGLIAGTTLVFVLSMGVYLTPVIMGGSFVTTLPMVMT
DLVRNQFDWSRAAAFAIVLLAFILIVLGLSSRAEKRIERMRGEGA

Sequences:

>Translated_285_residues
MALRTGRRIFWWGLIAPTFVLYALIFFVPLGILGSESLHAGAGGGLTSANYAAFFTDGVTLSIFWRTVRLSLLITAACIL
FGYPLALFMRRAGPVLRLVALTIVISPLLTSVVVRNVAWLLVLGREGMVNALLRQSGLIDGPLPLLYNTFGVVVGVTHVY
LAFLVMPVFSSLLSIDPSVEDAAASLGASRTTVFWRVTFPLSLPGLIAGTTLVFVLSMGVYLTPVIMGGSFVTTLPMVMT
DLVRNQFDWSRAAAFAIVLLAFILIVLGLSSRAEKRIERMRGEGA
>Mature_284_residues
ALRTGRRIFWWGLIAPTFVLYALIFFVPLGILGSESLHAGAGGGLTSANYAAFFTDGVTLSIFWRTVRLSLLITAACILF
GYPLALFMRRAGPVLRLVALTIVISPLLTSVVVRNVAWLLVLGREGMVNALLRQSGLIDGPLPLLYNTFGVVVGVTHVYL
AFLVMPVFSSLLSIDPSVEDAAASLGASRTTVFWRVTFPLSLPGLIAGTTLVFVLSMGVYLTPVIMGGSFVTTLPMVMTD
LVRNQFDWSRAAAFAIVLLAFILIVLGLSSRAEKRIERMRGEGA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=231, Percent_Identity=30.7359307359307, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787369, Length=240, Percent_Identity=30.4166666666667, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1787713, Length=244, Percent_Identity=28.2786885245902, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30771; Mature: 30640

Theoretical pI: Translated: 10.84; Mature: 10.84

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRTGRRIFWWGLIAPTFVLYALIFFVPLGILGSESLHAGAGGGLTSANYAAFFTDGVT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCH
LSIFWRTVRLSLLITAACILFGYPLALFMRRAGPVLRLVALTIVISPLLTSVVVRNVAWL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVLGREGMVNALLRQSGLIDGPLPLLYNTFGVVVGVTHVYLAFLVMPVFSSLLSIDPSVE
HHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
DAAASLGASRTTVFWRVTFPLSLPGLIAGTTLVFVLSMGVYLTPVIMGGSFVTTLPMVMT
HHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
DLVRNQFDWSRAAAFAIVLLAFILIVLGLSSRAEKRIERMRGEGA
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ALRTGRRIFWWGLIAPTFVLYALIFFVPLGILGSESLHAGAGGGLTSANYAAFFTDGVT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCH
LSIFWRTVRLSLLITAACILFGYPLALFMRRAGPVLRLVALTIVISPLLTSVVVRNVAWL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVLGREGMVNALLRQSGLIDGPLPLLYNTFGVVVGVTHVYLAFLVMPVFSSLLSIDPSVE
HHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
DAAASLGASRTTVFWRVTFPLSLPGLIAGTTLVFVLSMGVYLTPVIMGGSFVTTLPMVMT
HHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
DLVRNQFDWSRAAAFAIVLLAFILIVLGLSSRAEKRIERMRGEGA
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]