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The map label for this gene is siaP [H]

Identifier: 158424040

GI number: 158424040

Start: 2744114

End: 2745115

Strand: Direct

Name: siaP [H]

Synonym: AZC_2416

Alternate gene names: 158424040

Gene position: 2744114-2745115 (Clockwise)

Preceding gene: 158424038

Following gene: 158424041

Centisome position: 51.1

GC content: 62.97

Gene sequence:

>1002_bases
ATGTCCCTGTTTTCGCGCGGCCTCGCCGCGGCCTGCCTTGCTGCGTCAGCGCTCATCCTGTCGCCGCTGGCGGCCGATCC
CGCGGCCGCACAGACCAAGCTCAAGTGGGCTCACGTCTACGAGCCGTCCGAGCCGTTCCACACCGCCTCGGTCTGGGCCG
CCGGCGAGATCGCCAAGCGCACCAACGGCCGCTACCAGATCGACGTCTATCCCTCCTCGCAGCTCGGCAAGGAAAGCGAC
CTCAATCAGGGGCTGACGCTCGGCACCGTGGACATGATCATTTCCGGTTCGAGCTTCGCCGGAAAGGCCTATCCGCCCAT
CGGCGTGACCTATTATCCGTATACCTTCCGCGATGCCGACCATCTGCTCGCTTATGCCAAGAGCGACGTCTTCAAGGATC
TCGCCAAGGGCTATGAGGACAAGACGGGCAACCACATCGTGGCCGTCACCTATTACGGCGTGCGGCAGGCGACCTCGAAC
AAGCTGTTCAAGACCTGTGCGGAGATGAAGGGCCTCAAGATCCGCGTGCCCGATGCGCCCGCCTATCTCGCCATGCCCAA
GGCCTGCGGCGCCAACATCACCCCCATCGCCTTCGCCGAGGTCTATCTCGCGCTCCAGAACGGCACCGTGGACGCGCAGG
AAAACCCGCTGACCACCATCGAAGCCAAGAAGTTCTTCGAGGTGCAGAAGAACATCATCCTCACCGGTCACATCGTGGAC
CATCTGAACACGGTGCTCTCGCCCTCGCTCTGGGCCAAGCTCTCCCCCGAGGACCGCAAGATCTTCACCGACGTGGCGCA
GGAAGCGGCCGCCAAGGCCAGCAAGGAAGTGGCCGCCCGCGAGAAGGAACTGATCGCGGTCTTCAAGGAGAAGGGCCTCA
CCATCGTCGAGGTGGACACCAACGACTTCCGCGACACGGTGATGAAGAACATCCCGTTCGAGAGCTTCGGCTACCGCAAG
GCGGACTGGGAACGCATCCAGGCGCTCAAGAGCAGCATGTGA

Upstream 100 bases:

>100_bases
GGCATGGGCTGAGTACGATGGCCGCATTGACGGCGCGGCACGTCCGTCGATCAAAACGATTTAACCGGCTGCCAAGGCCG
GGCTCATCTGGAGGAAGCCC

Downstream 100 bases:

>100_bases
GACCGGCGGGCCGCGCCATTCGCGCGGCCGCCCTGCCCTGCCCCCCGGCGGACCTCAGGAGCCGCCGGGTCCTCTCCTGC
CTGACTGCCCGGAGTTCCGA

Product: C4-dicarboxylate ABC transporter substrate-binding protein

Products: NA

Alternate protein names: Extracytoplasmic solute receptor protein siaP; N-acetylneuraminic-binding protein; Neu5Ac-binding protein [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MSLFSRGLAAACLAASALILSPLAADPAAAQTKLKWAHVYEPSEPFHTASVWAAGEIAKRTNGRYQIDVYPSSQLGKESD
LNQGLTLGTVDMIISGSSFAGKAYPPIGVTYYPYTFRDADHLLAYAKSDVFKDLAKGYEDKTGNHIVAVTYYGVRQATSN
KLFKTCAEMKGLKIRVPDAPAYLAMPKACGANITPIAFAEVYLALQNGTVDAQENPLTTIEAKKFFEVQKNIILTGHIVD
HLNTVLSPSLWAKLSPEDRKIFTDVAQEAAAKASKEVAAREKELIAVFKEKGLTIVEVDTNDFRDTVMKNIPFESFGYRK
ADWERIQALKSSM

Sequences:

>Translated_333_residues
MSLFSRGLAAACLAASALILSPLAADPAAAQTKLKWAHVYEPSEPFHTASVWAAGEIAKRTNGRYQIDVYPSSQLGKESD
LNQGLTLGTVDMIISGSSFAGKAYPPIGVTYYPYTFRDADHLLAYAKSDVFKDLAKGYEDKTGNHIVAVTYYGVRQATSN
KLFKTCAEMKGLKIRVPDAPAYLAMPKACGANITPIAFAEVYLALQNGTVDAQENPLTTIEAKKFFEVQKNIILTGHIVD
HLNTVLSPSLWAKLSPEDRKIFTDVAQEAAAKASKEVAAREKELIAVFKEKGLTIVEVDTNDFRDTVMKNIPFESFGYRK
ADWERIQALKSSM
>Mature_332_residues
SLFSRGLAAACLAASALILSPLAADPAAAQTKLKWAHVYEPSEPFHTASVWAAGEIAKRTNGRYQIDVYPSSQLGKESDL
NQGLTLGTVDMIISGSSFAGKAYPPIGVTYYPYTFRDADHLLAYAKSDVFKDLAKGYEDKTGNHIVAVTYYGVRQATSNK
LFKTCAEMKGLKIRVPDAPAYLAMPKACGANITPIAFAEVYLALQNGTVDAQENPLTTIEAKKFFEVQKNIILTGHIVDH
LNTVLSPSLWAKLSPEDRKIFTDVAQEAAAKASKEVAAREKELIAVFKEKGLTIVEVDTNDFRDTVMKNIPFESFGYRKA
DWERIQALKSSM

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system siaPT involved in the uptake of sialic acid. This protein specifically binds sialic acid [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=310, Percent_Identity=34.1935483870968, Blast_Score=147, Evalue=7e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 36470; Mature: 36339

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFSRGLAAACLAASALILSPLAADPAAAQTKLKWAHVYEPSEPFHTASVWAAGEIAKR
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHH
TNGRYQIDVYPSSQLGKESDLNQGLTLGTVDMIISGSSFAGKAYPPIGVTYYPYTFRDAD
CCCEEEEEECCCHHCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEECCEEECCHH
HLLAYAKSDVFKDLAKGYEDKTGNHIVAVTYYGVRQATSNKLFKTCAEMKGLKIRVPDAP
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHCCCEEECCCCC
AYLAMPKACGANITPIAFAEVYLALQNGTVDAQENPLTTIEAKKFFEVQKNIILTGHIVD
CCEECCHHCCCCCCHHHHHHEEEEEECCCCCCCCCCCEEHHHHHHHHHHHCEEEEHHHHH
HLNTVLSPSLWAKLSPEDRKIFTDVAQEAAAKASKEVAAREKELIAVFKEKGLTIVEVDT
HHHHHHCCHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
NDFRDTVMKNIPFESFGYRKADWERIQALKSSM
CHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SLFSRGLAAACLAASALILSPLAADPAAAQTKLKWAHVYEPSEPFHTASVWAAGEIAKR
CHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHH
TNGRYQIDVYPSSQLGKESDLNQGLTLGTVDMIISGSSFAGKAYPPIGVTYYPYTFRDAD
CCCEEEEEECCCHHCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEECCEEECCHH
HLLAYAKSDVFKDLAKGYEDKTGNHIVAVTYYGVRQATSNKLFKTCAEMKGLKIRVPDAP
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHCCCEEECCCCC
AYLAMPKACGANITPIAFAEVYLALQNGTVDAQENPLTTIEAKKFFEVQKNIILTGHIVD
CCEECCHHCCCCCCHHHHHHEEEEEECCCCCCCCCCCEEHHHHHHHHHHHCEEEEHHHHH
HLNTVLSPSLWAKLSPEDRKIFTDVAQEAAAKASKEVAAREKELIAVFKEKGLTIVEVDT
HHHHHHCCHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
NDFRDTVMKNIPFESFGYRKADWERIQALKSSM
CHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA