Click here to switch to the map view.

The map label for this gene is ycjG [H]

Identifier: 158423836

GI number: 158423836

Start: 2527546

End: 2528523

Strand: Reverse

Name: ycjG [H]

Synonym: AZC_2212

Alternate gene names: 158423836

Gene position: 2528523-2527546 (Counterclockwise)

Preceding gene: 158423841

Following gene: 158423833

Centisome position: 47.09

GC content: 68.92

Gene sequence:

>978_bases
ATGCCCCAACTCAGTGTCCGCGTCGAACGCTTTCCCATCGCCGGAACCTTCACCATTGCCCGCGGCTCCAAGACCGAAGC
GGTCGTGGTGGTGGCCCGGCTGGAGGCGGACGGCCGCGCCGGCGAGGGCGAGTGCGTGCCCTATGCCCGCTATGGCGAGA
CCGTCGAAGGGACGGTCAAGACCTTGGAGGATTTGGCGAAAGCGGTATCTGACGGCCTTGATCGGACGGCCCTGCAGGGC
GCCGTTCCCGCCGGTGCCGCCCGAAACGCACTCGACTGCGCCTTCTGGGATCTGGAGGCCAAGACGCACGGCACTCCTGT
CGCGAGCCGCCTCGGCCTCGTCCCGCCCGCCCCCTTGGTGACCGCTTATACGCTCAGCCTTGATTCGCCCGAGGCCATGG
CCCGTGCGGCCAGGGCGGCCGGCCGCCCCCTGCTCAAGCTGAAGCTCGGAGCGGAAGGCGACGAAGAGCGACTTGCCGCG
ATCCGCGCAGCCGTGCCGCAGGCGCGCCTCATCGTCGATGCCAATGAGGGCTGGTCGCCGGATACGCTGGAGGCGCGCAT
GGCGGCCTGCGCCCGCTTCGGGGTCGAACTCATCGAGCAGCCTTTGCCCGCCGACAATGATGCGATGTTGGCGCAGGTGA
AGCGCCCCGTCACCATATGCGCCGACGAGAGCGTGCACGACCGTCACGGCCTTGCCGCCCTGCGCGATCGCTATGATGCC
ATCAACATCAAGCTCGACAAGGCGGGCGGCCTCACGGAAGCGCTTGCATTGGCGCAGGAGGGCGAGGCGCTCGGCTTCAA
ACTGATGGTCGGCTGCATGCTGGCAACATCTCTCGCCATGGCGCCGGCTCTCCTGTTGGCCCAGAAGGCGCAGGTGGTGG
ACCTCGACGGGCCTCTGCTGCTCGCCCGCGACCGGCAGCCGGGCATCCGATACGACGGCGCCCTCGCCTACCCCCCGACG
CCCGACCTCTGGGGCTGA

Upstream 100 bases:

>100_bases
CCATGCTCATACTCGCCCGGCCTTCCGGCGTCGAGATGCTGCGGCTGCGCAGCACGCAGCGTCAGCCCGTTTCTTTCAGT
GTGAACAGCGCTTTAACCAA

Downstream 100 bases:

>100_bases
AGCGCGGCGGAGGCAAGCGCCGCCACCAGGCCCACAGCACCGAGCGCCACAGAGAGATAAAATGCGTTCGCCCCGAAGGC
CTGGAAGAGCGGCCCTGACG

Product: L-Ala-D/L-Glu epimerase

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MPQLSVRVERFPIAGTFTIARGSKTEAVVVVARLEADGRAGEGECVPYARYGETVEGTVKTLEDLAKAVSDGLDRTALQG
AVPAGAARNALDCAFWDLEAKTHGTPVASRLGLVPPAPLVTAYTLSLDSPEAMARAARAAGRPLLKLKLGAEGDEERLAA
IRAAVPQARLIVDANEGWSPDTLEARMAACARFGVELIEQPLPADNDAMLAQVKRPVTICADESVHDRHGLAALRDRYDA
INIKLDKAGGLTEALALAQEGEALGFKLMVGCMLATSLAMAPALLLAQKAQVVDLDGPLLLARDRQPGIRYDGALAYPPT
PDLWG

Sequences:

>Translated_325_residues
MPQLSVRVERFPIAGTFTIARGSKTEAVVVVARLEADGRAGEGECVPYARYGETVEGTVKTLEDLAKAVSDGLDRTALQG
AVPAGAARNALDCAFWDLEAKTHGTPVASRLGLVPPAPLVTAYTLSLDSPEAMARAARAAGRPLLKLKLGAEGDEERLAA
IRAAVPQARLIVDANEGWSPDTLEARMAACARFGVELIEQPLPADNDAMLAQVKRPVTICADESVHDRHGLAALRDRYDA
INIKLDKAGGLTEALALAQEGEALGFKLMVGCMLATSLAMAPALLLAQKAQVVDLDGPLLLARDRQPGIRYDGALAYPPT
PDLWG
>Mature_324_residues
PQLSVRVERFPIAGTFTIARGSKTEAVVVVARLEADGRAGEGECVPYARYGETVEGTVKTLEDLAKAVSDGLDRTALQGA
VPAGAARNALDCAFWDLEAKTHGTPVASRLGLVPPAPLVTAYTLSLDSPEAMARAARAAGRPLLKLKLGAEGDEERLAAI
RAAVPQARLIVDANEGWSPDTLEARMAACARFGVELIEQPLPADNDAMLAQVKRPVTICADESVHDRHGLAALRDRYDAI
NIKLDKAGGLTEALALAQEGEALGFKLMVGCMLATSLAMAPALLLAQKAQVVDLDGPLLLARDRQPGIRYDGALAYPPTP
DLWG

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=314, Percent_Identity=48.7261146496815, Blast_Score=271, Evalue=5e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 34304; Mature: 34173

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQLSVRVERFPIAGTFTIARGSKTEAVVVVARLEADGRAGEGECVPYARYGETVEGTVK
CCCCEEEEEEECCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCHHHCCCCHHHHHH
TLEDLAKAVSDGLDRTALQGAVPAGAARNALDCAFWDLEAKTHGTPVASRLGLVPPAPLV
HHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCCCCCCE
TAYTLSLDSPEAMARAARAAGRPLLKLKLGAEGDEERLAAIRAAVPQARLIVDANEGWSP
EEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCC
DTLEARMAACARFGVELIEQPLPADNDAMLAQVKRPVTICADESVHDRHGLAALRDRYDA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCE
INIKLDKAGGLTEALALAQEGEALGFKLMVGCMLATSLAMAPALLLAQKAQVVDLDGPLL
EEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEE
LARDRQPGIRYDGALAYPPTPDLWG
EEECCCCCCCCCCEECCCCCCCCCC
>Mature Secondary Structure 
PQLSVRVERFPIAGTFTIARGSKTEAVVVVARLEADGRAGEGECVPYARYGETVEGTVK
CCCEEEEEEECCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCHHHCCCCHHHHHH
TLEDLAKAVSDGLDRTALQGAVPAGAARNALDCAFWDLEAKTHGTPVASRLGLVPPAPLV
HHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCCCCCCE
TAYTLSLDSPEAMARAARAAGRPLLKLKLGAEGDEERLAAIRAAVPQARLIVDANEGWSP
EEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCC
DTLEARMAACARFGVELIEQPLPADNDAMLAQVKRPVTICADESVHDRHGLAALRDRYDA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCE
INIKLDKAGGLTEALALAQEGEALGFKLMVGCMLATSLAMAPALLLAQKAQVVDLDGPLL
EEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEE
LARDRQPGIRYDGALAYPPTPDLWG
EEECCCCCCCCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]