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The map label for this gene is lexA [H]

Identifier: 158423825

GI number: 158423825

Start: 2516378

End: 2517088

Strand: Reverse

Name: lexA [H]

Synonym: AZC_2201

Alternate gene names: 158423825

Gene position: 2517088-2516378 (Counterclockwise)

Preceding gene: 158423826

Following gene: 158423823

Centisome position: 46.88

GC content: 68.21

Gene sequence:

>711_bases
ATGCTCACCCGCAAGCAGTATGATCTTCTGCGCTTCATCCATGAGCGCCTGAAGGAAACGGGCGTGCCTCCCTCCTTCGA
CGAGATGAAGGAGGCATTGGATCTGCGCTCCAAATCCGGCATCCATCGCCTCATCACGGCCCTTGAGGAACGCGGCTTCA
TCCGCCGCCTGCCGAACCGCGCGCGGGCGCTGGAAGTGGTGCGCCTGCCAGATAGCGCGGCGCCGGGTCTCGCGGCCGCC
CGCAGCGGCGGGCGCGGCTTCTCGCCGAGCGTCATCGAAGGCAGTCTCGGGCGCGTGCGCCCGGTGGTGGATGACGAGGA
ACCGGCCGCGGTGGTGGCCGTGCCGGTGATGGGCCGCATCGCGGCCGGCTCGCCCATCTCGGCCATCCAGACTCGCAGCA
ACACGCTCAACCTGCCTCCCGAAATGCTCGGCACGGGCGAGCATTTCGCCCTTGAGGTGCGTGGCGACAGCATGATCGAG
GCCGGCATTCTCGACGGCGACACGGTGCTCATCCGCAAGTGCGACACGGCCGACACCGGCGACATCATCGTGGCGCTGGT
GGATGACGAGGAAGCCACCCTCAAACGCCTGCGCCGCAAGGGCGCCTCCATCGCCCTTGAGGCGGCCAATCCCGCCTATG
AGACGCGCATCTTCGGCCCCGACCGCGTGCGCATCCAGGGGCGGCTCGTGGGGCTCATCCGCCGCTACTGA

Upstream 100 bases:

>100_bases
ACAAGAAACAGGTACATGATGTTCCTGATTTGTTTCGCAGGGCGCGGAGCGGATCGTCTCCCGGTCCGATGGGCCTGGTC
GCAAGGGGCGGAACCACACG

Downstream 100 bases:

>100_bases
GGGCGCGTTCGGAAGCGCGGTTTCCGAGAGCCGGATTGTTTTGGGACAGTGAGATGCAGCGGTCCGACGGGCGGACCATC
AGGGTTCCTCCTGCGCATCG

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MLTRKQYDLLRFIHERLKETGVPPSFDEMKEALDLRSKSGIHRLITALEERGFIRRLPNRARALEVVRLPDSAAPGLAAA
RSGGRGFSPSVIEGSLGRVRPVVDDEEPAAVVAVPVMGRIAAGSPISAIQTRSNTLNLPPEMLGTGEHFALEVRGDSMIE
AGILDGDTVLIRKCDTADTGDIIVALVDDEEATLKRLRRKGASIALEAANPAYETRIFGPDRVRIQGRLVGLIRRY

Sequences:

>Translated_236_residues
MLTRKQYDLLRFIHERLKETGVPPSFDEMKEALDLRSKSGIHRLITALEERGFIRRLPNRARALEVVRLPDSAAPGLAAA
RSGGRGFSPSVIEGSLGRVRPVVDDEEPAAVVAVPVMGRIAAGSPISAIQTRSNTLNLPPEMLGTGEHFALEVRGDSMIE
AGILDGDTVLIRKCDTADTGDIIVALVDDEEATLKRLRRKGASIALEAANPAYETRIFGPDRVRIQGRLVGLIRRY
>Mature_236_residues
MLTRKQYDLLRFIHERLKETGVPPSFDEMKEALDLRSKSGIHRLITALEERGFIRRLPNRARALEVVRLPDSAAPGLAAA
RSGGRGFSPSVIEGSLGRVRPVVDDEEPAAVVAVPVMGRIAAGSPISAIQTRSNTLNLPPEMLGTGEHFALEVRGDSMIE
AGILDGDTVLIRKCDTADTGDIIVALVDDEEATLKRLRRKGASIALEAANPAYETRIFGPDRVRIQGRLVGLIRRY

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family [H]

Homologues:

Organism=Escherichia coli, GI1790476, Length=235, Percent_Identity=25.9574468085106, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24 [H]

EC number: =3.4.21.88 [H]

Molecular weight: Translated: 25740; Mature: 25740

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRKQYDLLRFIHERLKETGVPPSFDEMKEALDLRSKSGIHRLITALEERGFIRRLPNR
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHCCCH
ARALEVVRLPDSAAPGLAAARSGGRGFSPSVIEGSLGRVRPVVDDEEPAAVVAVPVMGRI
HHEEEEEECCCCCCCCCHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEEECCCCCC
AAGSPISAIQTRSNTLNLPPEMLGTGEHFALEVRGDSMIEAGILDGDTVLIRKCDTADTG
CCCCCHHHHHCCCCCCCCCHHHHCCCCEEEEEECCCCEEEECCCCCCEEEEEECCCCCCC
DIIVALVDDEEATLKRLRRKGASIALEAANPAYETRIFGPDRVRIQGRLVGLIRRY
CEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCEEEECCCCEEEEHHHHHHHHHCC
>Mature Secondary Structure
MLTRKQYDLLRFIHERLKETGVPPSFDEMKEALDLRSKSGIHRLITALEERGFIRRLPNR
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHCCCH
ARALEVVRLPDSAAPGLAAARSGGRGFSPSVIEGSLGRVRPVVDDEEPAAVVAVPVMGRI
HHEEEEEECCCCCCCCCHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEEECCCCCC
AAGSPISAIQTRSNTLNLPPEMLGTGEHFALEVRGDSMIEAGILDGDTVLIRKCDTADTG
CCCCCHHHHHCCCCCCCCCHHHHCCCCEEEEEECCCCEEEECCCCCCEEEEEECCCCCCC
DIIVALVDDEEATLKRLRRKGASIALEAANPAYETRIFGPDRVRIQGRLVGLIRRY
CEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCEEEECCCCEEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA