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The map label for this gene is gltX [H]

Identifier: 158423823

GI number: 158423823

Start: 2511895

End: 2513349

Strand: Reverse

Name: gltX [H]

Synonym: AZC_2199

Alternate gene names: 158423823

Gene position: 2513349-2511895 (Counterclockwise)

Preceding gene: 158423825

Following gene: 158423822

Centisome position: 46.81

GC content: 68.04

Gene sequence:

>1455_bases
TTGCCCACCCGCTTTCCTCCCACGTCCGGCCTTTACGAGATGTCGCAGATCGTCACCCGCTTTGCTCCCTCCCCCACGGG
CTTTCTGCATATTGGCGGCGCCCGCACGGCGCTGTTCAACTGGCTCTATGCCAAGGCCAAGGGCGGCAAGATGCTGCTGC
GGATCGAGGACACCGACCGCCAGCGCTCCACCAAGGAGGCCATCGACGCCATTCTGGAGGGCCTGACCTGGCTCGGCATC
GATTGGGACGGGGACGTCATCTACCAGTTCGCCCGTGCCGAGCGGCACCGGCAGGCGGTGGAGGAGATGCTGTCCCGCGG
CAATGCCTATCCCTGCTATGCGACGCCCGAGGAGCTCGACGAGATGCGCGAGCTCGCCCGCAAGGAGGGCCGCCCACCGC
GCTATGACGGCCGCTGGCGCGACCGCCCGGCCTCGGAGCGCCCCACCGACCGCAAGCCGGTCATCCGCCTGCGCGCCCCG
CAGGAGGGCGTCACGGTGATCGAGGATCAGGTGCAGGGCACCGTCACCTTCCCGAACAAGGATCTCGACGATCTCGTCCT
GCTGCGCTCGGACGGCACGCCCACTTATATGCTGGCGGTGGTGGTGGACGACCATGACATGGGCGTCACCAACATCATCC
GCGGCGACGACCATCTCACCAACGCCGCCCGCCAGACGCAGATCTACAATGCGCTCGGCTGGGACGTGCCGCGCATGGCG
CACATTCCGCTCATCCATGGGCCGGACGGCGCCAAGCTCTCCAAGCGCCACGGCGCGCTGGGCGTCGATGCCTATCGCGA
CATGGGCTATCTGCCCGCTGCCCTGCGCAACTATCTGGTCCGCCTCGGCTGGAGCCATGGCGATCAGGAAATCTTCTCCA
CGGAGGAGATGGTCGAGCATTTCGATCTGGATCGCGTCGGCCGCTCCGCCGCCCGCTTCGACTTCCAGAAGCTTGAGAAT
ATCAACGGCCATTACATGCGCGCCAGCTCCAACGCAGAGCTGTTCGACGCGGTGATGGGCCTGATTCCGCACCTGCCTGA
CGCCGAGCACCGCCTCGCCCGGCTGACGGACGCGCGCATGGAACAGCTGCGCGCGGCCCTTCCGGGGCTCAAGGAGCGCG
CCAAGACGCTGACCGAGCTGCTGGACGGTGCCGAGTTCATCTTCGCCACCCGGCCGCTGCTGCTGGAGGAGAAGGCGCAA
GCCGTGCTCACGGCGGAGGCGCGCGCCCATATCGCCGCCATGATCCCGCAACTTGAGGCGGCCGACTGGTCCGCCGCCGG
CACCGAGACCGTGGTGCGGGCCTATGCGGAGCAGGCAGGCGTGAAGCTCGGTGCCGTGGCGCAGCCGCTGCGCGCCGCCC
TCACCGGCCGCACCACCTCTCCGCCCGTCTTCGACGTCTTCGCCGTTCTCGGCCGCGAGGAAAGCCTCGCCCGCCTCAAG
GACCAGACCGCATGA

Upstream 100 bases:

>100_bases
CGGCCGTGTTCCTTCGCTCCCGCGCCTATGCTACACGGGCGCCTCGCATGCGCCACGGCTTCGTGCGCGTCGGCCTGCTG
GCAGGCCTCACAGGGGCGTC

Downstream 100 bases:

>100_bases
GGAGCGCGCTTCGCATGGTGGACAGCCCGAACAAGGATCGCGCGCCCGGTTTCGGCCGGCGCACCCCGGCCGCCGATCCG
GTGACCGGCGAGCTCTATCT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MPTRFPPTSGLYEMSQIVTRFAPSPTGFLHIGGARTALFNWLYAKAKGGKMLLRIEDTDRQRSTKEAIDAILEGLTWLGI
DWDGDVIYQFARAERHRQAVEEMLSRGNAYPCYATPEELDEMRELARKEGRPPRYDGRWRDRPASERPTDRKPVIRLRAP
QEGVTVIEDQVQGTVTFPNKDLDDLVLLRSDGTPTYMLAVVVDDHDMGVTNIIRGDDHLTNAARQTQIYNALGWDVPRMA
HIPLIHGPDGAKLSKRHGALGVDAYRDMGYLPAALRNYLVRLGWSHGDQEIFSTEEMVEHFDLDRVGRSAARFDFQKLEN
INGHYMRASSNAELFDAVMGLIPHLPDAEHRLARLTDARMEQLRAALPGLKERAKTLTELLDGAEFIFATRPLLLEEKAQ
AVLTAEARAHIAAMIPQLEAADWSAAGTETVVRAYAEQAGVKLGAVAQPLRAALTGRTTSPPVFDVFAVLGREESLARLK
DQTA

Sequences:

>Translated_484_residues
MPTRFPPTSGLYEMSQIVTRFAPSPTGFLHIGGARTALFNWLYAKAKGGKMLLRIEDTDRQRSTKEAIDAILEGLTWLGI
DWDGDVIYQFARAERHRQAVEEMLSRGNAYPCYATPEELDEMRELARKEGRPPRYDGRWRDRPASERPTDRKPVIRLRAP
QEGVTVIEDQVQGTVTFPNKDLDDLVLLRSDGTPTYMLAVVVDDHDMGVTNIIRGDDHLTNAARQTQIYNALGWDVPRMA
HIPLIHGPDGAKLSKRHGALGVDAYRDMGYLPAALRNYLVRLGWSHGDQEIFSTEEMVEHFDLDRVGRSAARFDFQKLEN
INGHYMRASSNAELFDAVMGLIPHLPDAEHRLARLTDARMEQLRAALPGLKERAKTLTELLDGAEFIFATRPLLLEEKAQ
AVLTAEARAHIAAMIPQLEAADWSAAGTETVVRAYAEQAGVKLGAVAQPLRAALTGRTTSPPVFDVFAVLGREESLARLK
DQTA
>Mature_483_residues
PTRFPPTSGLYEMSQIVTRFAPSPTGFLHIGGARTALFNWLYAKAKGGKMLLRIEDTDRQRSTKEAIDAILEGLTWLGID
WDGDVIYQFARAERHRQAVEEMLSRGNAYPCYATPEELDEMRELARKEGRPPRYDGRWRDRPASERPTDRKPVIRLRAPQ
EGVTVIEDQVQGTVTFPNKDLDDLVLLRSDGTPTYMLAVVVDDHDMGVTNIIRGDDHLTNAARQTQIYNALGWDVPRMAH
IPLIHGPDGAKLSKRHGALGVDAYRDMGYLPAALRNYLVRLGWSHGDQEIFSTEEMVEHFDLDRVGRSAARFDFQKLENI
NGHYMRASSNAELFDAVMGLIPHLPDAEHRLARLTDARMEQLRAALPGLKERAKTLTELLDGAEFIFATRPLLLEEKAQA
VLTAEARAHIAAMIPQLEAADWSAAGTETVVRAYAEQAGVKLGAVAQPLRAALTGRTTSPPVFDVFAVLGREESLARLKD
QTA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=493, Percent_Identity=32.657200811359, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI4826960, Length=131, Percent_Identity=35.1145038167939, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788743, Length=472, Percent_Identity=43.4322033898305, Blast_Score=390, Evalue=1e-109,
Organism=Escherichia coli, GI226510925, Length=258, Percent_Identity=37.984496124031, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1786895, Length=132, Percent_Identity=31.8181818181818, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71993394, Length=488, Percent_Identity=31.3524590163934, Blast_Score=208, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI17543332, Length=132, Percent_Identity=31.0606060606061, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324540, Length=490, Percent_Identity=32.2448979591837, Blast_Score=236, Evalue=8e-63,
Organism=Saccharomyces cerevisiae, GI6321192, Length=253, Percent_Identity=30.4347826086957, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6324742, Length=130, Percent_Identity=31.5384615384615, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24665321, Length=492, Percent_Identity=33.5365853658537, Blast_Score=251, Evalue=6e-67,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 54012; Mature: 53881

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTRFPPTSGLYEMSQIVTRFAPSPTGFLHIGGARTALFNWLYAKAKGGKMLLRIEDTDR
CCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCH
QRSTKEAIDAILEGLTWLGIDWDGDVIYQFARAERHRQAVEEMLSRGNAYPCYATPEELD
HHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
EMRELARKEGRPPRYDGRWRDRPASERPTDRKPVIRLRAPQEGVTVIEDQVQGTVTFPNK
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHEECCCCEEEECCCC
DLDDLVLLRSDGTPTYMLAVVVDDHDMGVTNIIRGDDHLTNAARQTQIYNALGWDVPRMA
CCCCEEEEECCCCCEEEEEEEEECCCCCHHHEECCCHHHHHHHHHHHHHHHHCCCCCCCC
HIPLIHGPDGAKLSKRHGALGVDAYRDMGYLPAALRNYLVRLGWSHGDQEIFSTEEMVEH
CCCEEECCCCCCHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FDLDRVGRSAARFDFQKLENINGHYMRASSNAELFDAVMGLIPHLPDAEHRLARLTDARM
HCHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EQLRAALPGLKERAKTLTELLDGAEFIFATRPLLLEEKAQAVLTAEARAHIAAMIPQLEA
HHHHHHCCCHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ADWSAAGTETVVRAYAEQAGVKLGAVAQPLRAALTGRTTSPPVFDVFAVLGREESLARLK
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHH
DQTA
CCCC
>Mature Secondary Structure 
PTRFPPTSGLYEMSQIVTRFAPSPTGFLHIGGARTALFNWLYAKAKGGKMLLRIEDTDR
CCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCH
QRSTKEAIDAILEGLTWLGIDWDGDVIYQFARAERHRQAVEEMLSRGNAYPCYATPEELD
HHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
EMRELARKEGRPPRYDGRWRDRPASERPTDRKPVIRLRAPQEGVTVIEDQVQGTVTFPNK
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHEECCCCEEEECCCC
DLDDLVLLRSDGTPTYMLAVVVDDHDMGVTNIIRGDDHLTNAARQTQIYNALGWDVPRMA
CCCCEEEEECCCCCEEEEEEEEECCCCCHHHEECCCHHHHHHHHHHHHHHHHCCCCCCCC
HIPLIHGPDGAKLSKRHGALGVDAYRDMGYLPAALRNYLVRLGWSHGDQEIFSTEEMVEH
CCCEEECCCCCCHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FDLDRVGRSAARFDFQKLENINGHYMRASSNAELFDAVMGLIPHLPDAEHRLARLTDARM
HCHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EQLRAALPGLKERAKTLTELLDGAEFIFATRPLLLEEKAQAVLTAEARAHIAAMIPQLEA
HHHHHHCCCHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ADWSAAGTETVVRAYAEQAGVKLGAVAQPLRAALTGRTTSPPVFDVFAVLGREESLARLK
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHH
DQTA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA