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The map label for this gene is hipO [H]

Identifier: 158423699

GI number: 158423699

Start: 2387785

End: 2388948

Strand: Reverse

Name: hipO [H]

Synonym: AZC_2075

Alternate gene names: 158423699

Gene position: 2388948-2387785 (Counterclockwise)

Preceding gene: 158423700

Following gene: 158423698

Centisome position: 44.49

GC content: 66.84

Gene sequence:

>1164_bases
ATGCCTGCATTGCCCGAAATCGAAGCTTTCGCCGACGATCTCGTCGCCATCCGCCGCGACATCCATGCCCATCCGGAAAT
CGGCTTCGAGGAAGTGCGCACCTCCGCGCTGGTGGCCGAGAAGCTCGCCTCCTGGGGCGTCGAGGTGCATCGTGGCATCG
GCAAGACCGGCGTCGTCGGCGTGCTGCACGGCGCCAAGGGTCCGGGCCGTCGCATCGGCCTGCGCGCCGACATGGACGCC
CTGCCCATGGACGAAGCGACCAACCTGCCTTTCTCCTCCAAATATCCCGGCAAATTCCACGGCTGCGGCCATGACGGCCA
CACCACCATGCTGCTGGGCGCAGCCCGCTATCTTGCCAGCAACAACGACTTTGCCGGCACCGTCCATTTCATCTTCCAGC
CGGCCGAGGAGGGTCTCGGCGGCGCCCGCGCCATGCTGGCTGACAAGCTGTTCGAGCGCTTCCCCTGTGATGAAGTCTAT
GGCCTGCACAATGCGCCGAACATCGAGGCCGGCAAGGTGCGCATCTTCGCCGGCCCGGCCCAGGCCGGGGCCGACTTCTT
CGACATCAAGATCACGGGCCGCGGCTCCCATGGCGCGCGCCCGGAGGTCTCCCGCGACCCGGTGATCGCCGCGACCGCTT
TGGTGCAGGCGCTGCAATCCATCGTCAGCCGAAATGCCGATCCCCAGCACGCCGCCGTGCTGTCGGTGACCAAGATCCAT
TCCGGTTCGGCCTATAACGTCATTCCCCAGGAAGCGACGCTCGGCGGCACCACCCGCTTCTTCGATGCGGCCACCGGCGA
ACTCATGCGCCAGCGCATGCGCACCGTCGCCGCCGGCATCGCCGCCACCTTCGAGATGGAGATCGACGTGGAGATCCGCT
CCATCTTCGACGTGCTGGTGAATCACGAGGCCCAGAGCGACGCCGCCGCCAAGAGCATCGCCAAGATCGTCGGTGCCGAG
AACCTGCTCACCGACGTGGCCCCGATCATGGGCAGCGAGGACTTCGCCGATATGCTGCGGGCTGTCCCCGGTGCCTATTT
CTGGGTCGGCCATGGTGGCGACGTGCCGGTGCACAATCCTGGCTTCGTCCTGGACGAGGCCATCCTGCCCATCGGCTCCA
GCCTGCTGGCGACGGTCGCCACCGACCGCCTCGCGGCGGCCTGA

Upstream 100 bases:

>100_bases
CAGCCTGCCGTGGAAGCATCTGCGCCCCGGCGTGCGACTGGAACCCAACGGCCCGGCCTTCGCCTGACCTTCTCCCCCAA
AGCTGACCTGAGACGGACCC

Downstream 100 bases:

>100_bases
GGCACGCCATGGACCTCTCTGCCCTGCCGTTCGACACGACATCCATGCTGGCGCGCCTGCGCCCCTGGATCGAGTGCGAG
AGCCCTACCTATGACCCGGC

Product: amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MPALPEIEAFADDLVAIRRDIHAHPEIGFEEVRTSALVAEKLASWGVEVHRGIGKTGVVGVLHGAKGPGRRIGLRADMDA
LPMDEATNLPFSSKYPGKFHGCGHDGHTTMLLGAARYLASNNDFAGTVHFIFQPAEEGLGGARAMLADKLFERFPCDEVY
GLHNAPNIEAGKVRIFAGPAQAGADFFDIKITGRGSHGARPEVSRDPVIAATALVQALQSIVSRNADPQHAAVLSVTKIH
SGSAYNVIPQEATLGGTTRFFDAATGELMRQRMRTVAAGIAATFEMEIDVEIRSIFDVLVNHEAQSDAAAKSIAKIVGAE
NLLTDVAPIMGSEDFADMLRAVPGAYFWVGHGGDVPVHNPGFVLDEAILPIGSSLLATVATDRLAAA

Sequences:

>Translated_387_residues
MPALPEIEAFADDLVAIRRDIHAHPEIGFEEVRTSALVAEKLASWGVEVHRGIGKTGVVGVLHGAKGPGRRIGLRADMDA
LPMDEATNLPFSSKYPGKFHGCGHDGHTTMLLGAARYLASNNDFAGTVHFIFQPAEEGLGGARAMLADKLFERFPCDEVY
GLHNAPNIEAGKVRIFAGPAQAGADFFDIKITGRGSHGARPEVSRDPVIAATALVQALQSIVSRNADPQHAAVLSVTKIH
SGSAYNVIPQEATLGGTTRFFDAATGELMRQRMRTVAAGIAATFEMEIDVEIRSIFDVLVNHEAQSDAAAKSIAKIVGAE
NLLTDVAPIMGSEDFADMLRAVPGAYFWVGHGGDVPVHNPGFVLDEAILPIGSSLLATVATDRLAAA
>Mature_386_residues
PALPEIEAFADDLVAIRRDIHAHPEIGFEEVRTSALVAEKLASWGVEVHRGIGKTGVVGVLHGAKGPGRRIGLRADMDAL
PMDEATNLPFSSKYPGKFHGCGHDGHTTMLLGAARYLASNNDFAGTVHFIFQPAEEGLGGARAMLADKLFERFPCDEVYG
LHNAPNIEAGKVRIFAGPAQAGADFFDIKITGRGSHGARPEVSRDPVIAATALVQALQSIVSRNADPQHAAVLSVTKIHS
GSAYNVIPQEATLGGTTRFFDAATGELMRQRMRTVAAGIAATFEMEIDVEIRSIFDVLVNHEAQSDAAAKSIAKIVGAEN
LLTDVAPIMGSEDFADMLRAVPGAYFWVGHGGDVPVHNPGFVLDEAILPIGSSLLATVATDRLAAA

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=429, Percent_Identity=27.039627039627, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 40971; Mature: 40840

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPALPEIEAFADDLVAIRRDIHAHPEIGFEEVRTSALVAEKLASWGVEVHRGIGKTGVVG
CCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEE
VLHGAKGPGRRIGLRADMDALPMDEATNLPFSSKYPGKFHGCGHDGHTTMLLGAARYLAS
EEECCCCCCCEECEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
NNDFAGTVHFIFQPAEEGLGGARAMLADKLFERFPCDEVYGLHNAPNIEAGKVRIFAGPA
CCCCCEEEEEEEECHHHCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEEEECCC
QAGADFFDIKITGRGSHGARPEVSRDPVIAATALVQALQSIVSRNADPQHAAVLSVTKIH
CCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEEC
SGSAYNVIPQEATLGGTTRFFDAATGELMRQRMRTVAAGIAATFEMEIDVEIRSIFDVLV
CCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHHH
NHEAQSDAAAKSIAKIVGAENLLTDVAPIMGSEDFADMLRAVPGAYFWVGHGGDVPVHNP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCCC
GFVLDEAILPIGSSLLATVATDRLAAA
CCHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PALPEIEAFADDLVAIRRDIHAHPEIGFEEVRTSALVAEKLASWGVEVHRGIGKTGVVG
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEE
VLHGAKGPGRRIGLRADMDALPMDEATNLPFSSKYPGKFHGCGHDGHTTMLLGAARYLAS
EEECCCCCCCEECEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
NNDFAGTVHFIFQPAEEGLGGARAMLADKLFERFPCDEVYGLHNAPNIEAGKVRIFAGPA
CCCCCEEEEEEEECHHHCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEEEECCC
QAGADFFDIKITGRGSHGARPEVSRDPVIAATALVQALQSIVSRNADPQHAAVLSVTKIH
CCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEEC
SGSAYNVIPQEATLGGTTRFFDAATGELMRQRMRTVAAGIAATFEMEIDVEIRSIFDVLV
CCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHHH
NHEAQSDAAAKSIAKIVGAENLLTDVAPIMGSEDFADMLRAVPGAYFWVGHGGDVPVHNP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCCC
GFVLDEAILPIGSSLLATVATDRLAAA
CCHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]