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The map label for this gene is gltJ [H]

Identifier: 158423550

GI number: 158423550

Start: 2228425

End: 2229192

Strand: Reverse

Name: gltJ [H]

Synonym: AZC_1926

Alternate gene names: 158423550

Gene position: 2229192-2228425 (Counterclockwise)

Preceding gene: 158423551

Following gene: 158423549

Centisome position: 41.51

GC content: 64.84

Gene sequence:

>768_bases
ATGAAGTATGACTGGAGCTGGAGCGTCTTTCTCGACCTCTCGTCGGATGGCGTTCACAGCTATCTCTATAACCTCGGCCT
TGGTGTGTTGTGGACACTGGGCCTCACCGGCGCGACGGCGGCGGGGGCCCTTGTGTTCGGCTCCATTCTGGGCGTCTTCC
GCACCACCCGGGCGCCGGTGCTGAATGCCATCGGCGCCACCTATGTGGAGATCTTCCGCAACATCCCGCTGCTGGTGCAG
ATGTTCCTTTGGTACTTCGTGCTGCCCGAGGTGCTGCCCAAGGCGATCGGTCAGGCCATCAAAGGGATGCCGGCTCCCTG
GAACGCCTTCGTCCCCGCCTTGCTGTGCCTCACCTTCTACGGGTCGGCCCGGATCGGGGAACAAATGCGCTCAGGGATCC
AGTCGTTGCCGCGCGGCCAGCTTCAGGCGGCGCTGGCGCTGGGGCTGACGCCGGTAATGGCCTATCGGCATGTGGTCCTG
CCCGAAGCCTTCCGCCTCATGATCCCGCCGCTCACCTCCGAGTTCATGGCGATCGTGAAATATTCCTCCGTCGCCATGAC
CATCGGCCTGCTGGAGCTGACCGGCCAGGCGCGCGCGATGCAGGAAGCGAGCTTCCATATCTTCGAGGCCTTCAGCGCCG
CGACCGTGGGTTATCTGGTGATCAACGGCTGCATCGTGCTGGCCATGCGCTGGCTCGAACGCCGCCTCGCCGTGCCCGGC
CTCATCGGTGCTGCGGCCAGCAGTCCGGCGCGGGTGGCGCAGTCATGA

Upstream 100 bases:

>100_bases
GCGGGCCCTTGCCAAGCCGACGGATTCCGGAGATCCGGCCGACTATCAGTGAGCGCGGTCCGCGCTCCACACCCCGAGCG
GCATCTGGCGGATCCGGGCG

Downstream 100 bases:

>100_bases
ACGGGTCCTTCGACTTCGGCGCCATCGCGGCCGCCCTGCCCTACCTGCTCGGCGACGGGCTCGGTCTCACCTTCGGCCTC
ACGGTGCTTGCGGGCGCCAT

Product: glutamate/aspartate transporter permease 2

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKYDWSWSVFLDLSSDGVHSYLYNLGLGVLWTLGLTGATAAGALVFGSILGVFRTTRAPVLNAIGATYVEIFRNIPLLVQ
MFLWYFVLPEVLPKAIGQAIKGMPAPWNAFVPALLCLTFYGSARIGEQMRSGIQSLPRGQLQAALALGLTPVMAYRHVVL
PEAFRLMIPPLTSEFMAIVKYSSVAMTIGLLELTGQARAMQEASFHIFEAFSAATVGYLVINGCIVLAMRWLERRLAVPG
LIGAAASSPARVAQS

Sequences:

>Translated_255_residues
MKYDWSWSVFLDLSSDGVHSYLYNLGLGVLWTLGLTGATAAGALVFGSILGVFRTTRAPVLNAIGATYVEIFRNIPLLVQ
MFLWYFVLPEVLPKAIGQAIKGMPAPWNAFVPALLCLTFYGSARIGEQMRSGIQSLPRGQLQAALALGLTPVMAYRHVVL
PEAFRLMIPPLTSEFMAIVKYSSVAMTIGLLELTGQARAMQEASFHIFEAFSAATVGYLVINGCIVLAMRWLERRLAVPG
LIGAAASSPARVAQS
>Mature_255_residues
MKYDWSWSVFLDLSSDGVHSYLYNLGLGVLWTLGLTGATAAGALVFGSILGVFRTTRAPVLNAIGATYVEIFRNIPLLVQ
MFLWYFVLPEVLPKAIGQAIKGMPAPWNAFVPALLCLTFYGSARIGEQMRSGIQSLPRGQLQAALALGLTPVMAYRHVVL
PEAFRLMIPPLTSEFMAIVKYSSVAMTIGLLELTGQARAMQEASFHIFEAFSAATVGYLVINGCIVLAMRWLERRLAVPG
LIGAAASSPARVAQS

Specific function: Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786874, Length=244, Percent_Identity=43.8524590163934, Blast_Score=207, Evalue=4e-55,
Organism=Escherichia coli, GI1787030, Length=240, Percent_Identity=30.8333333333333, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788226, Length=209, Percent_Identity=29.1866028708134, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786873, Length=210, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787086, Length=220, Percent_Identity=30.9090909090909, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI48994930, Length=125, Percent_Identity=39.2, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082239, Length=162, Percent_Identity=32.0987654320988, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788645, Length=173, Percent_Identity=29.4797687861272, Blast_Score=74, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 27615; Mature: 27615

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYDWSWSVFLDLSSDGVHSYLYNLGLGVLWTLGLTGATAAGALVFGSILGVFRTTRAPV
CCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LNAIGATYVEIFRNIPLLVQMFLWYFVLPEVLPKAIGQAIKGMPAPWNAFVPALLCLTFY
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GSARIGEQMRSGIQSLPRGQLQAALALGLTPVMAYRHVVLPEAFRLMIPPLTSEFMAIVK
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
YSSVAMTIGLLELTGQARAMQEASFHIFEAFSAATVGYLVINGCIVLAMRWLERRLAVPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LIGAAASSPARVAQS
HHHHCCCCCHHHCCC
>Mature Secondary Structure
MKYDWSWSVFLDLSSDGVHSYLYNLGLGVLWTLGLTGATAAGALVFGSILGVFRTTRAPV
CCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LNAIGATYVEIFRNIPLLVQMFLWYFVLPEVLPKAIGQAIKGMPAPWNAFVPALLCLTFY
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GSARIGEQMRSGIQSLPRGQLQAALALGLTPVMAYRHVVLPEAFRLMIPPLTSEFMAIVK
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
YSSVAMTIGLLELTGQARAMQEASFHIFEAFSAATVGYLVINGCIVLAMRWLERRLAVPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LIGAAASSPARVAQS
HHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]