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The map label for this gene is lysR [H]

Identifier: 158423497

GI number: 158423497

Start: 2146249

End: 2147214

Strand: Reverse

Name: lysR [H]

Synonym: AZC_1873

Alternate gene names: 158423497

Gene position: 2147214-2146249 (Counterclockwise)

Preceding gene: 158423503

Following gene: 158423496

Centisome position: 39.99

GC content: 63.87

Gene sequence:

>966_bases
ATGTCATTCCAAGGCAGGCAGGGCGCCGAACAGATCGAGCTGCGCCACCTACGTTATTTCCTCGCCACCGCCGAGGAGCT
GAACTACAGGCGGGCCGCCGAACGGCTGCGCATCGCCCAGCCCGGCCTCAGCCAGCAGATCAAGAAGCTGGAGGAGATCG
TCGGCACGCCTCTGTTTGACCGCACCCGCCGCACGGTTCGATTGACCCTCGCGGGAGAGCTTTTCATGCACGAGGCGCGC
AAGACACTGGCCCATGCCGAGAGCGCCATGCTCGTGGCCCGCCGCGCCGGGCGCGGCGAGGTCGGACGGATCGCCATCGG
CTATGTCGGGTCGGCGGCCTATACTGGGGTTCTTACGACCATGGTCGGGAAGTTTCGGTCGAGCTATCCGGAGGTGGAGC
TGCAGATTTCCGAAATGGAAATGCAGCAGCAGCTCGATCAACTCGACCAGGGACGGCTGGATATCGGCTTTATCCGTCCG
CCGGTGCCACTTCCAGTCGGCATCGGTACCATCCCGGTCCTGTTCGAGGATATCGTTCTGGCTCTGCCGGAGGCTCACCC
ACAGGGCGCGAGCGAGCAGGTCGCACTCGCCTCGCTCAATGAGGACATTTTCATAACGCCACACCATCCGCCGGAAGTCA
GTTTTCTCAAGCACACCACTTCTGCGTGCCGAATGGCAGGTTTCTTTCCCAGGCTCGGTCCTCAGGGGCGCGACTTCGTC
ACAATAGCGAGCATGGTTGCGGTTGGGCTCGGCGTGGCCTTGGTGCCCCAATCGGTCCGCTGCATTCAGGTTCCAGGCCT
CCAATATCGGCCGATCGCCGATGCCAAGGTGTTGGCGGAGCTTGCCGTAGCATTCCGGCGGAGCGAGCCGTCACCCGTCG
CACGGGCCTTCGCGGCCCACGCCCGGCGTTCTGCCTTCTCTGGATCCGAGCCGTCCCCGGTGCGCGGGCGGTCTAAGAAG
TCGTGA

Upstream 100 bases:

>100_bases
GCGTCGATCGATGAAAGCTGTCCATCAATATTGCTGCCCGAGGTTGGATCAGTCCAATATGCAGCCGGCGGCCTTTTGAG
AACCCAGGGTTATTAGACAC

Downstream 100 bases:

>100_bases
TTATCAAGAAAGCCAAAACCGCATATTGGACTGATGGCTGAAACTGCGCCGATAATCTCCAGACCAACAAAGGTGCAAGT
CGGCGCTCCAAGCCGGGCGG

Product: regulatory protein

Products: NA

Alternate protein names: Als operon regulatory protein [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSFQGRQGAEQIELRHLRYFLATAEELNYRRAAERLRIAQPGLSQQIKKLEEIVGTPLFDRTRRTVRLTLAGELFMHEAR
KTLAHAESAMLVARRAGRGEVGRIAIGYVGSAAYTGVLTTMVGKFRSSYPEVELQISEMEMQQQLDQLDQGRLDIGFIRP
PVPLPVGIGTIPVLFEDIVLALPEAHPQGASEQVALASLNEDIFITPHHPPEVSFLKHTTSACRMAGFFPRLGPQGRDFV
TIASMVAVGLGVALVPQSVRCIQVPGLQYRPIADAKVLAELAVAFRRSEPSPVARAFAAHARRSAFSGSEPSPVRGRSKK
S

Sequences:

>Translated_321_residues
MSFQGRQGAEQIELRHLRYFLATAEELNYRRAAERLRIAQPGLSQQIKKLEEIVGTPLFDRTRRTVRLTLAGELFMHEAR
KTLAHAESAMLVARRAGRGEVGRIAIGYVGSAAYTGVLTTMVGKFRSSYPEVELQISEMEMQQQLDQLDQGRLDIGFIRP
PVPLPVGIGTIPVLFEDIVLALPEAHPQGASEQVALASLNEDIFITPHHPPEVSFLKHTTSACRMAGFFPRLGPQGRDFV
TIASMVAVGLGVALVPQSVRCIQVPGLQYRPIADAKVLAELAVAFRRSEPSPVARAFAAHARRSAFSGSEPSPVRGRSKK
S
>Mature_320_residues
SFQGRQGAEQIELRHLRYFLATAEELNYRRAAERLRIAQPGLSQQIKKLEEIVGTPLFDRTRRTVRLTLAGELFMHEARK
TLAHAESAMLVARRAGRGEVGRIAIGYVGSAAYTGVLTTMVGKFRSSYPEVELQISEMEMQQQLDQLDQGRLDIGFIRPP
VPLPVGIGTIPVLFEDIVLALPEAHPQGASEQVALASLNEDIFITPHHPPEVSFLKHTTSACRMAGFFPRLGPQGRDFVT
IASMVAVGLGVALVPQSVRCIQVPGLQYRPIADAKVLAELAVAFRRSEPSPVARAFAAHARRSAFSGSEPSPVRGRSKKS

Specific function: Regulates the expression of the alsSD operon for acetoin biosynthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=293, Percent_Identity=35.4948805460751, Blast_Score=177, Evalue=9e-46,
Organism=Escherichia coli, GI2367136, Length=280, Percent_Identity=33.2142857142857, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1788887, Length=288, Percent_Identity=33.3333333333333, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI157672245, Length=288, Percent_Identity=32.6388888888889, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1790399, Length=247, Percent_Identity=29.9595141700405, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788297, Length=263, Percent_Identity=26.9961977186312, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081904, Length=180, Percent_Identity=32.7777777777778, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI145693105, Length=187, Percent_Identity=28.8770053475936, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790208, Length=255, Percent_Identity=29.0196078431373, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787806, Length=290, Percent_Identity=24.8275862068966, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787601, Length=240, Percent_Identity=27.5, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787530, Length=280, Percent_Identity=23.5714285714286, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790262, Length=301, Percent_Identity=26.578073089701, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788748, Length=151, Percent_Identity=25.8278145695364, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787589, Length=125, Percent_Identity=36.8, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35197; Mature: 35066

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFQGRQGAEQIELRHLRYFLATAEELNYRRAAERLRIAQPGLSQQIKKLEEIVGTPLFD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHH
RTRRTVRLTLAGELFMHEARKTLAHAESAMLVARRAGRGEVGRIAIGYVGSAAYTGVLTT
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHH
MVGKFRSSYPEVELQISEMEMQQQLDQLDQGRLDIGFIRPPVPLPVGIGTIPVLFEDIVL
HHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
ALPEAHPQGASEQVALASLNEDIFITPHHPPEVSFLKHTTSACRMAGFFPRLGPQGRDFV
HCCCCCCCCCCCCEEHEECCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHH
TIASMVAVGLGVALVPQSVRCIQVPGLQYRPIADAKVLAELAVAFRRSEPSPVARAFAAH
HHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ARRSAFSGSEPSPVRGRSKKS
HHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SFQGRQGAEQIELRHLRYFLATAEELNYRRAAERLRIAQPGLSQQIKKLEEIVGTPLFD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHH
RTRRTVRLTLAGELFMHEARKTLAHAESAMLVARRAGRGEVGRIAIGYVGSAAYTGVLTT
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHH
MVGKFRSSYPEVELQISEMEMQQQLDQLDQGRLDIGFIRPPVPLPVGIGTIPVLFEDIVL
HHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
ALPEAHPQGASEQVALASLNEDIFITPHHPPEVSFLKHTTSACRMAGFFPRLGPQGRDFV
HCCCCCCCCCCCCEEHEECCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHH
TIASMVAVGLGVALVPQSVRCIQVPGLQYRPIADAKVLAELAVAFRRSEPSPVARAFAAH
HHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ARRSAFSGSEPSPVRGRSKKS
HHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377 [H]