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The map label for this gene is acyI [H]

Identifier: 158423496

GI number: 158423496

Start: 2144365

End: 2146122

Strand: Reverse

Name: acyI [H]

Synonym: AZC_1872

Alternate gene names: 158423496

Gene position: 2146122-2144365 (Counterclockwise)

Preceding gene: 158423497

Following gene: 158423495

Centisome position: 39.97

GC content: 64.79

Gene sequence:

>1758_bases
GTGTGCCGTCGAACTGCGAGGGGTCTTCCCCCGGGGCGGTGGCTGGAATCAGGCGGACCGAAAATGGATTGGCGTAGCCG
CGCGGGGACTTCCTTCATTTGCGAGAAGACAGCCGTCACTGCCAGCCGGGGTATGGCGGTCACCAACCACCCCTTGGCCT
CCGCCGCCGCGGTCGAGATGATGGCGGCCGGGGGCAATGCAGTGGATGCCGCCATCGCAGCGTTATTCACGCTCACGGTG
GTCGAGCCGATGATGGTTGGCATCTTTGGAGGTGGAGCGGCCCTGATCCGTCTCGCTGACGGCCGGGAAGTCGTGCTTGA
TGGTCTCTCGACGGCCCCCGCGGCTGCGCGTTCGGATAGCTTTACGCCAATCTCCGACACCTGGCCCGATTACATGGAGG
CGAAGGGCCGCGCCAACCGCGTTGGCCCCAAAGCGGTGGCGGTTCCCGGAACGCTGAAAGCGTGGTGTGAGGCGGCTGGA
CAGTTCGGCCGGCTGCCACTGTCCGATCTCATTGCGCCGGCTATCCGCCACGCCGAGCGGGGTTTTCGCCTTTCTCCCTA
TCTCGCCACCTGCATCGCGGAAATTGCCCCGGACCTCGCGCTCGACGCAGCCATCGCCGCTGTTTTCCTGCCGAATGGCC
GCCCACTCGGCGCCGGAGACCTTCTGATCCAGAAAGACTATGCCAATACGCTGCGCCTCATTGCTGAAGAAGGGCCACAG
GTGCTTTACGGCGGTGAGCTTGGTCGCCGCGTCGCAGATTATTTGGCCGGTGCCGGTTCCTTCCTTCGCTTCGAGGACTT
GGAGAGATACCGGACGGTCACGCGTGAACCCGTCCGGGGCACCTATCGCGGCTTTGAGATCGTCGGACCGCCGCCGCCTT
GTGCAGGCGGCGTACATACGCTGCAGATCCTTAATCTCCTTGAGGCCTACAATATCGCCGATTCCGGCTTCGGGACCCGC
GAAACGCTGCACCTCGTCCTCGAAGCGCTGAAGATCGCGGCAGCTGACAGGAGGGCTGTCACCGCGGACCCCGCCTTTGT
GGATGTGCCTGTCGAACGGCTGATCTCGAAGGCCTATGCTGACCAGCGTCGAATGGAGATCGACGTCGCACGGGCGAGCA
CGTTCGAGGCCCGGATTCGTTCAAACGAGTCCGCCAACACGACCCATGTCACGGTGGCTGACGCCGAGGGCAATATCGTT
ACGTCGACCCAGACCATCAACAGCCTGTTCGGCGCCCGCATCATCATCCCCGGCACGGGCATCATTCCGAACAACTACAT
GTACCTGTTCGATCCCCATCCCGGAAATGCGCTCTCGCTGGAACCGGGCAAGCGGATCACCAGCGGCATCACGGCCCTCA
TCGGGAAGAAGGAGGGACGTCCGGTCTTCGCGCTGGGCCTGCCGGGAGCGCACCGGATCCCCGCCTCGGCCATGCAGGCC
GTTTTGAACCTCATCGACCATGGAATGGACGTTCAGGAGGCGGTGGAAGCGCCCCGCGTCTTCACCTGGGGCCAAGAGGT
GGAGATCGAGACCAGCGTTCCGGTCGAGATCCGTGACACGCTCGCGACCATGGGGCATCGCGTCGTCGGCGTGGACCACG
TGGCAGGTGGCATGTGCGCGATCGGGTTTTCAGACGATGGTCGGATGACAGGCGCGGCCTGCTGGCGCGCCGACGGCGTC
CCCATGGGGCTGGGCGGCGGCAGCGCCCGGGCCGGCACCTCGTTCTGGCCGGATCCCCGTCGTGGACGGGTCTACTGA

Upstream 100 bases:

>100_bases
TGGACTGATGGCTGAAACTGCGCCGATAATCTCCAGACCAACAAAGGTGCAAGTCGGCGCTCCAAGCCGGGCGGACGACC
CACTGCGCACATAACCAGAG

Downstream 100 bases:

>100_bases
ACAAATAAGGCCGGCGGCCACCGAGGGGAATCATCATGCAGATCAACCGACGCGAAGCATTGATATGGTCCGCCGCCACG
TTGCTGGCGACCACCCGCTC

Product: gamma-glutamyltranspeptidase

Products: NA

Alternate protein names: Cephalosporin acylase; GL-7ACA acylase; Gamma-glutamyltranspeptidase; GGT [H]

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MCRRTARGLPPGRWLESGGPKMDWRSRAGTSFICEKTAVTASRGMAVTNHPLASAAAVEMMAAGGNAVDAAIAALFTLTV
VEPMMVGIFGGGAALIRLADGREVVLDGLSTAPAAARSDSFTPISDTWPDYMEAKGRANRVGPKAVAVPGTLKAWCEAAG
QFGRLPLSDLIAPAIRHAERGFRLSPYLATCIAEIAPDLALDAAIAAVFLPNGRPLGAGDLLIQKDYANTLRLIAEEGPQ
VLYGGELGRRVADYLAGAGSFLRFEDLERYRTVTREPVRGTYRGFEIVGPPPPCAGGVHTLQILNLLEAYNIADSGFGTR
ETLHLVLEALKIAAADRRAVTADPAFVDVPVERLISKAYADQRRMEIDVARASTFEARIRSNESANTTHVTVADAEGNIV
TSTQTINSLFGARIIIPGTGIIPNNYMYLFDPHPGNALSLEPGKRITSGITALIGKKEGRPVFALGLPGAHRIPASAMQA
VLNLIDHGMDVQEAVEAPRVFTWGQEVEIETSVPVEIRDTLATMGHRVVGVDHVAGGMCAIGFSDDGRMTGAACWRADGV
PMGLGGGSARAGTSFWPDPRRGRVY

Sequences:

>Translated_585_residues
MCRRTARGLPPGRWLESGGPKMDWRSRAGTSFICEKTAVTASRGMAVTNHPLASAAAVEMMAAGGNAVDAAIAALFTLTV
VEPMMVGIFGGGAALIRLADGREVVLDGLSTAPAAARSDSFTPISDTWPDYMEAKGRANRVGPKAVAVPGTLKAWCEAAG
QFGRLPLSDLIAPAIRHAERGFRLSPYLATCIAEIAPDLALDAAIAAVFLPNGRPLGAGDLLIQKDYANTLRLIAEEGPQ
VLYGGELGRRVADYLAGAGSFLRFEDLERYRTVTREPVRGTYRGFEIVGPPPPCAGGVHTLQILNLLEAYNIADSGFGTR
ETLHLVLEALKIAAADRRAVTADPAFVDVPVERLISKAYADQRRMEIDVARASTFEARIRSNESANTTHVTVADAEGNIV
TSTQTINSLFGARIIIPGTGIIPNNYMYLFDPHPGNALSLEPGKRITSGITALIGKKEGRPVFALGLPGAHRIPASAMQA
VLNLIDHGMDVQEAVEAPRVFTWGQEVEIETSVPVEIRDTLATMGHRVVGVDHVAGGMCAIGFSDDGRMTGAACWRADGV
PMGLGGGSARAGTSFWPDPRRGRVY
>Mature_585_residues
MCRRTARGLPPGRWLESGGPKMDWRSRAGTSFICEKTAVTASRGMAVTNHPLASAAAVEMMAAGGNAVDAAIAALFTLTV
VEPMMVGIFGGGAALIRLADGREVVLDGLSTAPAAARSDSFTPISDTWPDYMEAKGRANRVGPKAVAVPGTLKAWCEAAG
QFGRLPLSDLIAPAIRHAERGFRLSPYLATCIAEIAPDLALDAAIAAVFLPNGRPLGAGDLLIQKDYANTLRLIAEEGPQ
VLYGGELGRRVADYLAGAGSFLRFEDLERYRTVTREPVRGTYRGFEIVGPPPPCAGGVHTLQILNLLEAYNIADSGFGTR
ETLHLVLEALKIAAADRRAVTADPAFVDVPVERLISKAYADQRRMEIDVARASTFEARIRSNESANTTHVTVADAEGNIV
TSTQTINSLFGARIIIPGTGIIPNNYMYLFDPHPGNALSLEPGKRITSGITALIGKKEGRPVFALGLPGAHRIPASAMQA
VLNLIDHGMDVQEAVEAPRVFTWGQEVEIETSVPVEIRDTLATMGHRVVGVDHVAGGMCAIGFSDDGRMTGAACWRADGV
PMGLGGGSARAGTSFWPDPRRGRVY

Specific function: Besides the cephalosporin acylase I activity which converts GL-7ACA into 7-ACA; this enzyme displays some gamma glutamyltranspeptidase activity [H]

COG id: COG0405

COG function: function code E; Gamma-glutamyltransferase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI73915092, Length=461, Percent_Identity=29.2841648590022, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI73915090, Length=461, Percent_Identity=29.2841648590022, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI73915096, Length=461, Percent_Identity=29.2841648590022, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI73915094, Length=461, Percent_Identity=29.2841648590022, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI113429324, Length=459, Percent_Identity=28.322440087146, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI113429326, Length=464, Percent_Identity=28.2327586206897, Blast_Score=146, Evalue=7e-35,
Organism=Homo sapiens, GI109148539, Length=510, Percent_Identity=28.4313725490196, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI153266885, Length=504, Percent_Identity=26.7857142857143, Blast_Score=137, Evalue=4e-32,
Organism=Homo sapiens, GI153266901, Length=505, Percent_Identity=26.7326732673267, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI153266907, Length=501, Percent_Identity=25.9481037924152, Blast_Score=123, Evalue=4e-28,
Organism=Escherichia coli, GI1789856, Length=539, Percent_Identity=33.5807050092764, Blast_Score=288, Evalue=5e-79,
Organism=Caenorhabditis elegans, GI17506189, Length=544, Percent_Identity=28.125, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI193208247, Length=541, Percent_Identity=26.0628465804067, Blast_Score=148, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI193208245, Length=541, Percent_Identity=26.0628465804067, Blast_Score=148, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI212646519, Length=502, Percent_Identity=28.8844621513944, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI72000294, Length=502, Percent_Identity=28.8844621513944, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17542894, Length=511, Percent_Identity=27.2015655577299, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17564448, Length=428, Percent_Identity=25.9345794392523, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323330, Length=511, Percent_Identity=23.4833659491194, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI161077880, Length=471, Percent_Identity=28.8747346072187, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI45551474, Length=471, Percent_Identity=28.6624203821656, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24642670, Length=471, Percent_Identity=28.6624203821656, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24642666, Length=471, Percent_Identity=28.6624203821656, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI161077878, Length=471, Percent_Identity=28.8747346072187, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI18859893, Length=520, Percent_Identity=29.8076923076923, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI161077496, Length=511, Percent_Identity=26.4187866927593, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI18859741, Length=532, Percent_Identity=24.0601503759398, Blast_Score=106, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000101 [H]

Pfam domain/function: PF01019 G_glu_transpept [H]

EC number: =2.3.2.2 [H]

Molecular weight: Translated: 62059; Mature: 62059

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00462 G_GLU_TRANSPEPTIDASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRRTARGLPPGRWLESGGPKMDWRSRAGTSFICEKTAVTASRGMAVTNHPLASAAAVEM
CCCCCCCCCCCCCHHHCCCCCCCCHHCCCCEEEEHHHHHHHHCCEEEECCCCHHHHHHHH
MAAGGNAVDAAIAALFTLTVVEPMMVGIFGGGAALIRLADGREVVLDGLSTAPAAARSDS
HHCCCCHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCEEEECCCCCCCCCCCCCC
FTPISDTWPDYMEAKGRANRVGPKAVAVPGTLKAWCEAAGQFGRLPLSDLIAPAIRHAER
CCCCCCCCHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
GFRLSPYLATCIAEIAPDLALDAAIAAVFLPNGRPLGAGDLLIQKDYANTLRLIAEEGPQ
CCEECHHHHHHHHHHCCHHHHHHHHHEEECCCCCCCCCCCEEEEHHHHHHHHHHHHCCCC
VLYGGELGRRVADYLAGAGSFLRFEDLERYRTVTREPVRGTYRGFEIVGPPPPCAGGVHT
EEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHH
LQILNLLEAYNIADSGFGTRETLHLVLEALKIAAADRRAVTADPAFVDVPVERLISKAYA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCEEEHHHHHHHHHHHH
DQRRMEIDVARASTFEARIRSNESANTTHVTVADAEGNIVTSTQTINSLFGARIIIPGTG
HHHHEEEEEHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEHHHHHHHHHCCEEEECCCC
IIPNNYMYLFDPHPGNALSLEPGKRITSGITALIGKKEGRPVFALGLPGAHRIPASAMQA
CCCCCEEEEEECCCCCEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHH
VLNLIDHGMDVQEAVEAPRVFTWGQEVEIETSVPVEIRDTLATMGHRVVGVDHVAGGMCA
HHHHHHCCCCHHHHHCCCCEEECCCEEEEECCCCCHHHHHHHHHCCEEEEEHHCCCCEEE
IGFSDDGRMTGAACWRADGVPMGLGGGSARAGTSFWPDPRRGRVY
EEECCCCCCCCHHEEECCCCEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCRRTARGLPPGRWLESGGPKMDWRSRAGTSFICEKTAVTASRGMAVTNHPLASAAAVEM
CCCCCCCCCCCCCHHHCCCCCCCCHHCCCCEEEEHHHHHHHHCCEEEECCCCHHHHHHHH
MAAGGNAVDAAIAALFTLTVVEPMMVGIFGGGAALIRLADGREVVLDGLSTAPAAARSDS
HHCCCCHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCEEEECCCCCCCCCCCCCC
FTPISDTWPDYMEAKGRANRVGPKAVAVPGTLKAWCEAAGQFGRLPLSDLIAPAIRHAER
CCCCCCCCHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
GFRLSPYLATCIAEIAPDLALDAAIAAVFLPNGRPLGAGDLLIQKDYANTLRLIAEEGPQ
CCEECHHHHHHHHHHCCHHHHHHHHHEEECCCCCCCCCCCEEEEHHHHHHHHHHHHCCCC
VLYGGELGRRVADYLAGAGSFLRFEDLERYRTVTREPVRGTYRGFEIVGPPPPCAGGVHT
EEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHH
LQILNLLEAYNIADSGFGTRETLHLVLEALKIAAADRRAVTADPAFVDVPVERLISKAYA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCEEEHHHHHHHHHHHH
DQRRMEIDVARASTFEARIRSNESANTTHVTVADAEGNIVTSTQTINSLFGARIIIPGTG
HHHHEEEEEHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEHHHHHHHHHCCEEEECCCC
IIPNNYMYLFDPHPGNALSLEPGKRITSGITALIGKKEGRPVFALGLPGAHRIPASAMQA
CCCCCEEEEEECCCCCEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHH
VLNLIDHGMDVQEAVEAPRVFTWGQEVEIETSVPVEIRDTLATMGHRVVGVDHVAGGMCA
HHHHHHCCCCHHHHHCCCCEEECCCEEEEECCCCCHHHHHHHHHCCEEEEEHHCCCCEEE
IGFSDDGRMTGAACWRADGVPMGLGGGSARAGTSFWPDPRRGRVY
EEECCCCCCCCHHEEECCCCEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3680178 [H]